• The Journal of Korean Obstetrics and Gynecology
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• v.27 no.1
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• pp.41-64
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• 2014

Objectives: This study was to evaluate the effect of Jaeumkanghwa-tang (JEKHT) on the propylthiouracil (PTU)-induced rat hypothyroidism. Methods: Six groups, each of 8 rats per group were used in the present study - intact vehicle control, PTU control, Levothyroxine ($LT_4$), JEKHT 500, 250 and 125 mg/kg treated groups. JEKHT were administered once a day for 42 days as an oral dose of 500, 250 and 125 mg/kg, and hypothyroidism was induced by daily subcutaneous treatment of PTU 10 mg/kg for 28 days. The changes on the body and organ weight, serum hormone and lipid profiles, liver and testis antioxidant defense factors were observed with histopathology of organs. Results were compared with $LT_4$ 0.5 mg/kg intraperitoneally treated rats in this experiment. Results: PTU treatment, marked decrease of body weight, increases of thyroid weight, decreases of liver, testis, epididymis and prostate weights, decreases of serum Tri-iodothyronine ($T_3$), and Thyroxine ($T_4$) level with increase of serum Thyroid-stimulating hormone (TSH) level, decreases of serum testosterone and dihydrotestosterone (DHT) level with increases of serum Follicular stimulating hormone (FSH) level, increases of serum High density lipoprotein (HDL), decrease of triglyceride content, increase of serum Aspartate aminotransferase (AST) level, decreases of liver and testis antioxidant defense factors were observed. In addition, marked hyperplasia of follicular cells with decreases of follicular colloid contents and diameters was additionally demonstrated with the decrease of hepatocyte numbers per unit area due to hypertrophy of hepatocytes related to lipid droplet depositions, increase of a/oligospermatic epididymal tubules with epididymal atrophic changes, seminiferous tubular atrophy with decrease of stage I~II seminiferous tubules in testis, prostate tubular atrophic changes at histopathological inspections. However, these PTU induced hypothyroidism and related hepatic and male reproductive organ damages were favorably and dose-dependently inhibited by treatment of JEKHT 500, 250 and 125 mg/kg, and JEKHT also effectively regulated the PTU-induced abnormal antioxidant defense factor changes in the both liver and testis. Conclusions: JEKHT 500, 250 and 125 mg/kg dose-dependently inhibited PTU-induced hypothyroidism and related liver and male reproductive organ damages in rats.

• Applied Chemistry for Engineering
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• v.30 no.4
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• pp.493-498
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• 2019

In polymer exchange membrane fuel cells, it is crucial to fabricate a highly active and thin Pt catalyst layer for the smooth mass transport of dissolved oxygen and water. Although a highly loaded platinum (Pt) catalyst based on the hydrothermal synthesis has been reported in several studies, its growing behaviors and kinetics were yet to be understood. In this study, we investigated the growth of Pt crystal in suspension after the reduction step depending on a stirring time and evaluated the electrochemical activity. For only a couple of hours in the early stage, Pt colloids were adsorbed on the Pt-carbon catalyst and the Pt crystal was grown. After that, the small Pt colloid was formed by another nucleation step, which did not involve the growth of Pt crystal. We reveal that the Pt-Carbon catalyst with stirring for 6 h showed a high activity toward the oxygen reduction reaction.

• Journal of the Korean Applied Science and Technology
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• v.38 no.5
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• pp.1325-1334
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• 2021

This study reports the development of a manufacturing method of synthesizing colloidal gold using catalysts available for cosmetics and an anti-aging ampoule with skin improvement effects using it. Nano-colloidal gold was synthesized by using ascorbic acid and sodium borohydride as a reducing catalyst in hydrogen tetrachloroaurate tetrahydrate. It was confirmed that the particles became smaller as the mass of the content of ascorbic acid, which is a catalyst, increased. On the other hand, as the mass of sodium borohydride increased, the particle size tended to increase. In order to control the colloidal gold reaction rate, particles having 100 to 500 nm of a particle diameter distribution could be obtained using xanthan gum and hydroxyethylcellulose. The optimal synthesis conditions could be obtained by reacting for 1 to 4 hours at 18℃, a reduced pressure state of 20 to 75 mmHg, a stirring speed of 10~50 rpm. The synthesized colloidal gold had a unique smell of dark pink, pH = 5.5, specific gravity of 1.0032, and viscosity of 80 to 310 cps. As an application of skin care cosmetics, anti-aging ampoule has been developed, and it is expected to be used for various prescriptions and formulations using it.

• Journal of Yeungnam Medical Science
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• v.38 no.2
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• pp.136-141
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• 2021

Background: Postoperative acute kidney injury (AKI), which increases the risk of postoperative morbidity and mortality, poses a major concern to surgeons. We conducted this study to analyze the risk factors associated with the occurrence of AKI after orthopedic surgery. Methods: This was a retrospective study that included 351 patients who underwent total hip or knee replacement surgery at Inje University Haeundae Paik Hospital between January 2012 and December 2016. Results: AKI occurred in 13 (3.7%) of the 351 patients. The patients' preoperative estimated glomerular filtration rate (eGFR) was 66.66 ±34.02 mL/min/1.73 m² in the AKI group and 78.07±21.23 mL/min/1.73 m² in the non-AKI group. The hemoglobin levels were 11.21±1.65 g/dL in the AKI group and 12.39±1.52 g/dL in the non-AKI group. Hemoglobin level was related to increased risk of AKI (odds ratio [OR], 0.13; 95% confidence interval [CI], 0.02-0.68; p=0.016). Administration of crystalloid or colloid fluid alone and the perioperative amount of fluid did not show any significant relationship with AKI. Further analysis of the changes in eGFR was performed using a cutoff value of 7.54. The changes in eGFR were significantly related to decreased risk of AKI (OR, 0.74; 95% CI, 0.61-0.89; p=0.002). Conclusion: Renal function should be monitored closely after orthopedic surgery if patients have chronic kidney disease and low hemoglobin level. Predicting the likelihood of AKI occurrence, early treatment of high-risk patients, and monitoring perioperative laboratory test results, including eGFR, will help improve patient prognosis.

• Journal of the Korea Organic Resources Recycling Association
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• v.30 no.3
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• pp.5-12
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• 2022

This study suggests the effective recycling method of sludge waste from various industrial fields to synthesize uniform colloidal silica nanoparticles. In detail, polymers are removed from the sludge waste to attain sludge-extracted silica (s-SiO₂) micron-sized particles, and ammonia assisted sonication is applied to s-SiO₂, which has effectively extracted the silanol precursor. The nano-sized silica (n-SiO₂) particles are successfully synthesized by a typical sol-gel method using silanol precursor. Also, the yield amounts of n-SiO₂ are determined by the function of s-SiO₂ etching time. Finally, n-SiO₂-based slurry is synthesized for the practical CMP application. As a result, rough-surfaced semiconductor chip is successfully polished by the n-SiO₂-based slurry to exhibit the mirror-like clean surface. In this regard, sludge wastes are successfully prepared as valuable semicondutor grade materials.

• The Korea Journal of Herbology
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• v.34 no.3
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• pp.45-53
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• 2019

Objectives : In present study, we investigated a therapeutic effect of Astragali Radix on hypothyroidism rat model induced by 6-Propyl-2-thiouracil (PTU). Methods : Six-week-old male Sprague-Dawley rats were divided into five groups : Group one included the normal mice. Group two was administrated PTU. Group three and four were administrated the aqueous extract of Astragali Radix 150 and 300 mg/kg before start of PTU treatment. Group five (Positive control) was administrated with levothyroxine 0.5 mg/kg. During this moment the body weight, liver $H_2O_2$ and catalase (CAT) amount, serum thyroid hormone, serum asparte aminotransferase (AST) and alanine aminotransferase (ALT), gland weights were measured with histopathological changes of thyroid glands. These results were compared with levothyroxine 0.5 mg/kg treated rats. Results : The PTU treatment lead to marked decreases of body weight, level of thyroid hormone in serum and liver CAT activation. Also, PTU treatment increased thyroid gland weight, thyroid gland hormone TSH, liver $H_2O_2$ amount and level of AST in serum. On the other hands, the administration of Astragali Radix extract increased body weight gains and ameliorated histopathological changes of thyroid such as hyperplasia of follicular cells with of follicular colloid contents and sizes. In addition, the administration of Astragali Radix extract increased level of $T_4$ in serum, CAT activation in liver. Moreover, the administration of Astragali Radix extract decreased levels of TSH and AST in serum and $H_2O_2$ amount in liver Conclusions : This study suggests that Astragali Radix extract has therapeutic effects on hypothyroidism via promoting thyroid hormone production.

• Analytical Science and Technology
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• v.21 no.3
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• pp.237-243
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• 2008

Nano core shell structures of $TiO_2$ particles coated on surface of ZnO nanoparticles were prepared by the polymerized complex and sol-gel method. The average particle size of ZnO by the polymerized complex method showed 100 nm and the average particle size of $TiO_2$ by the sol-gel method showed below 10 nm. The average particle size of $ZnO@TiO_2$ nano core shell struture represented about 150 nm. The agglomeration between the ZnO particles using the polymerized complex method was highly controlled by the uniform absorption of $TiO_2$ colloid on the spherical ZnO surfaces. The driving force of heterogeneous bonding between ZnO and $TiO_2$ was induced by the Coulomb force. The ZnO and $TiO_2$ particles electrified with + and - charges, respectively, resulted in strong bonding by the difference of iso-electric point (IEP) when they laid neutrality pH area, depending on the heterogeneous surface electron electrified by the different zeta potential on the pH values.

• Journal of the Korea Organic Resources Recycling Association
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• v.17 no.4
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• pp.91-102
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• 2009

In this study, to estimate the transforming (runoff and leachate) rate of the organic fertilizer made of livestock resources to farm land, laboratory scale test was conducted and the results were obtained as follows: The runoff volume from farm land showed the tendency of increase according to the increase of rainfall intensity. The most rainfall leachated into the underground at the rainfall intensity of 20mm/hr, and rainfall of 5L or less leachated at the rainfall intensity of > 32.4 mm/hr. This shows that surface runoff largely depends on the rainfall intensity when soil characteristic and hardness are similar in each site. When liquid compost was fertilized, the surface runoff was similar with the results from the reactor fertilized by compost, and leachate flow was found to be lower than compost. The runoff ratio of contaminant parameters from farm land were BOD 0.00003,, $COD_{cr}$ 0.00006, TN 0.00056, TP 0.00011, TOC 0.00005, Especially, the runoff ratio of TN showed 10 folds higher than other parameters. On the other hand, the runoff ratio of SS showed higher value of 0.001, and colloid particles of soil caused this result rather than the leachate from compost fertilizer. At all ranges of rainfall intensity, fertilizer removal ratio by farm land was found to be 94.9~98.4% for compost and 85.8~98.1% for liquid compost in terms of BOD. For TN, it resulted in 96.6~98.4% for compost and 97.2~98.5% for liquid compost, and thus the most fertilizer from livestock resources were shown to be reduced through farm land application.

• The Korean Journal of Nuclear Medicine
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• v.30 no.3
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• pp.361-366
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• 1996

This study was to determine the effect of $Al^{3+}$ in $^{99m}Tc$ eluate from $^{99}Mo-^{99m}Tc$ generator on labeling efficiency and biodistribution of $^{99m}Tc$-MDP. The chromatographic analysis of $^{99m}Tc$-MDP preparations containing $Al^{3+}(0-62.5{\mu}g/ml)$ showed decreased labeling efficiency $^{99m}Tc$ pertechnetate and hydrolyzed reduced $^{99m}Tc$ fraction increased with increasing concentrations of aluminum. However, the chromatography system could not discern between hydrolyzed reduced $^{99m}Tc$ and $^{99m}Tc$ labeled colloid. $^{99m}Tc$-MDP preparations containing aluminum were relatively stable. Chromatographic analysis also confirmed that no significant differences were observed in the radiochemical purity of the filtered and the unfiltered $^{99m}Tc$-MDP preparations containing aluminum by $0.22{\mu}m$ syringe filter. In biodistribution data of ICR-mice, blood and heart uptake were increasing with increasing concentrations of aluminum, because of decreasing labeling efficiency of $^{99m}Tc$-MDP and increasing of $^{99m}Tc$ pertechnetate. However, liver and bone uptake were not significantly increased. In rat images no difference were observed at $5{\mu}g/ml\;Al^{3+}$ compare with at $0{\mu}g/ml\;Al^{3+}$, but at $10{\mu}g/ml\;Al^{3+}$ lumbar uptake was increased. As a practical conclusion, a concentration below $10{\mu}g/ml\;Al^{3+}$($10{\mu}g/ml\;Al^{3+}$ is the maximum allowed in pertechnetate eluate from $^{99}Mo-^{99m}Tc$ generator by USP.) in $^{99m}Tc$-MDP radiopharmaceutical result in low labeling efficiency. Radiochemical purity 90% of $^{99m}Tc$-MDP is the minimum allowed by USP. Therefore, when soft tissue uptake is observed in $^{99m}Tc$-MDP bone scan and labeling efficiency is above 90%, we can expect that $Al^{3+}$ in pertechnetated eluate is not the cause of soft tissue uptake.

• The Korean Journal of Nuclear Medicine Technology
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• v.14 no.1
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• pp.35-39
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• 2010

Purpose: The aim of this study was to investigate distribution of particle size in phytate kit and compare filtered method with non-filtered method using 200 nm filter for sentinel lymphoscintigraphy (SLS). Materials and Methods: Five phytate kit of having the same available period was measured by particle size analyzer. For in-vivo experiment, $^{99m}Tc$-phytate was injected intradermally at both foot to perform lymphoscintigraphy. Imaging was acquired at 1hour after injection. Region of interest (ROI) was drawn in inguinal and background area for analysis. RAW 264.7 cells (Murine macrophage cell) were prepared for measurement of celluar uptake as a representative of macrophages. Paired t-test was performed using SPSS (SPSS Inc, USA) for statistical analysis. Results: The size of most particle in Techne phytate kit was distributed in 130~650 nm(90.5 %). In-vivo study, the ROI analysis showed similar result between filtered and non-filtered sample, and the numerical value of count/pixel were $58.3{\pm}5.97$ and $60.2{\pm}4.88$. In-vitro study, cellular uptake study also showed no difference between filtered and non-filtered sample by gamma counting. Conclusion: The present study demonstrates that there was no meaning of 200 nm filtered method for SLS using $^{99m}Tc$-phytate.