• Journal of Acupuncture Research
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• 제24권5호
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• pp.171-183
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• 2007

Objectives : The purpose of this study was to analyze the effects of Erythrinae Cortex herbal-acupuncture solution(EC-HAS) at the Joksamni($ST_{36}$) of mice with collagen II-induced arthritis(CIA). Methods : The author performed several experimental procedures to observe the effects of the EC-HAS at the arthritis. The severity of arthritis, changes of cytokine level and antibody level, histological changes of the CIA mouse joint were analyzed. Results : 1. The incidence of arthritis and arthritis index were significantly decreased in the cases which were treated with the EC-HA. 2. Cartilage destruction and synovial cell proliferation were reduced, and the expression of the collagen fibers was similar with that of the normal group. 3. The levels of IL-6, $IFN-{\gamma}$, $TNF-{\alpha}$, $IL-1{\beta}$ in serum of the CIA mice which were treated with the EC-HA were significantly decreased compared with those of the normal group. 4. The levels of IgG, IgM and anti-collagen II in serum of CIA mice when they treated with the EC-HA were significantly decreased compared with those of the normal group. 5. The expression ratio of $CD4^+$ to $CD8^+$ cells in the EC-HA treated mice were maintained as much as the normal group of the lymph nodes in the CIA mice. 6. The $CD3e^+CD69^+$ and $CD11b^+Gr-1^+$ cell populations in the knee joint were significantly decreased in the EC-HA treated group. Conclusions : These results suggest that the EC-HA at the ST36 may be responsible roles to control on the synovial cell proliferation and to prevent the cartilage destruction in rheumatoid arthritis. These results will be important supporting evidence for the practical use of the EC-HA at rheumatoid arthritis clinic in the future.

• 한국융합학회논문지
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• 제7권4호
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• pp.67-73
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• 2016

본 연구는 야관문 추출물이 아토피 피부염에 미치는 효과를 알아보고자 시행하였다. 실험은 5개 군으로 분류하여 정상군, 아토피유발군(AD), 아토피 유발 후 LC 농도별 투여군(AD-LC: 100 mg/Kg, 300 mg/Kg, 500 mg/Kg)으로 구분하였다. AD군과 AD-LC군은 제모한 부위에 1% DNCB 용액 $1200{\mu}{\ell}$를 2주간 도포하고, 그 이후로는 2일 간격으로 0.1% DNCB 용액 $150{\mu}{\ell}$를 4 주간 도포하였다. 피부 두께는 아토피 피부염 증상을 나타내는 특징으로 AD군이 피부의 두께가 가장 두꺼웠으며, AD-LC 500군은 정상군과 같았다. 비만세포는 염증반응을 나타내는 세포로 AD군의 비만세포의 수가 가장 많았다. 콜라겐섬유와 탄력섬유는 정상적인 피부에서 많이 관찰되는데 AD-LC 500군에서 치밀하고 균일하게 나타났다. 이상의 연구결과로 보아 야관문 추출물이 피부조직의 염증에 의한 각화, 습진, 가려움증을 개선하는 항아토피 효능이 있다고 사료된다.

• 치과방사선
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• 제10권1호
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• pp.9-14
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• 1980

The author studied on the effects of X-ray irradiation to the development of periodontal ligament in gestation rats. They were irradiated in their abdomen with 100, 200 and 300 rads respectively in one shot irradiation with deep radiation therapy equipment (MAXIMAR 250-Ⅲ), In 7th, 14th, 21th and 28th day after delivery, those new born rats were respectively sacrificed with ether anesthesia and removed of their mandibles. After removal, those mandibles were fixed in 10% neutral buffer formalin, decalcified with 5% trichloroacetic acid for 5 days and embedded with paraffine. Staining was performed with H-E, Van Gieson, Mallory azan, Bielshowsky-Gomori silver stain and Halmi's oxytalan fiber stain. The results were as follows: 1. Before tooth eruption, all the fiber: components in dental sac were almost always oriented near the outer enamel epithelial \layer. But in irradiated new born rats, those collagen fiber orientation was more irregular than those of control groups, and this phenomenon was more severe in proportion to the amount of irradiation in the gestation period. 2. Before tooth eruption, the connective tissue fibers in periodontal ligament were stained with lighter in the irradiated groups than those of control groups. Oxytalan fibers of irradiated groups were thin and splitting pattern of their fiber morphology to compare with those of control groups. 3. After tooth eruption, the periodontal ligament fibers of irradiated groups were oriented functionally and their morphology was thick, fine and heavy staining. Oxytalan fibers were revealed with oblique parallel arrangement in the periodontal ligament of irradiated groups.

• 대한한의학회지
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• 제23권4호
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• pp.98-104
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• 2002

Objectives: Calpain, a calcium-dependent cysteine proteinase, may be one of the proteolytic enzymes that mediate cartilage degradation associated with rheumatoid arthritis. The object of this study is to ascertain immunohistochemically whether calpain is present in the inflamed joints of collagen-induced arthritis of rats, and examine the effect of Cortex Acanthopanacis Senticosi on the expression of calpain. Methods: Male Lewis rats, around 200g of body weight, were immunized with bovine type II collagen. After 3 weeks from first immunization, rats were divided into arthritic control (n=6) group and Cortex Acanthopanacis Senticosi-treated (n=6) group. Non-immunized rats served as the normal (n=6) group. All animals were sacrificed at 15 days post-treatment and tibiotarsal joints were removed. Calpain immunohistochemistry was performed on the midsagittal section of the tibiotarsal joint. Results: All animals of the control and treated groups showed ankylosing osteoarthritis. However, the animals of the treated group showed alleviation in the fibrous ankylosis, destruction of articular cartilage and destruction of subchondral bony tissue compared with the animals of the control group. Calpain was expressed in the chondrocyte lacunae of growing articular cartilage, in the skeletal muscle fibers, in the peripheral nerves, and in the vessel walls around the joints of all groups. In the control and treated groups, calpain was also expressed in proliferating synovial epithelia, subsynovial stroma cells, surface of articular cartilage, and fibrous pannus around destructive subchondral bony tissue. However, the expression density of calpain in the treated group was diminished compared with the control group, especially in surface of articular cartilage and fibrous pannus. Conclusions: These observations indicated that calpain plays an important role in the destruction of cartilage and bone in collagen-induced arthritis of rats, and also indicated that Cortex Acanthopanacis Senticosi inhibits the development of arthritis by decreasing the expression of calpain.

• Journal of Periodontal and Implant Science
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• 제26권1호
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• pp.68-79
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• 1996

To investigate the efficiency of a fibrous collagen membrane(FCM) composed of bovine skin type I atelocollagen as a carrier for BMP, partially purified bovine BMP/FCM($0.3mg/10{\times}5{\times}1mm$) composites were implanted into the dorsal subcutaneous tissue of rats. FCM alone was also implanted as a control. The implants were harvested at 1, 2, 3, and 10 weeks after implantation, then prepared for routine light microscopic observation. The FCM alone did not induce osteogenesis and revealed no specific foreign body reaction nor was there any definite resorptive evidence for 10 weeks after implantation, while the BMP/FCM composites induced favorable bone formation in a process that resembled an endochondral and direct ossification mode. At 10 weeks, the well formed bone confined to embedded collagen fibers revealed hematopoietic marrow between bony trabeculae without evidence of resorptive or degenerative changes . These findings support the suggestion that BMP may induce undifferentiated mesenchymal cells into either chondroblasts or osteoblasts or both independantly according to the chemico- physical characteristics of the carrier, which develops the endochondral and/or direct bone formation process, and suggest that the FCM may be a favorable carrier for BMP.

• Applied Microscopy
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• 제38권2호
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• pp.135-140
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• 2008

본 연구에서 분만 중에 사망한 여성미라의 자궁 내에 있는 태아미라의 두피를 주사전자현미경과 투과전자현미경을 사용하여 관찰하였다. 주사전자현미경상에서 태아 두피의 표면은 잘 보존된 상태로 관찰되었으며, 각질층에 각화세포도 모양을 그대로 유지하고 있었다. 또한 땀샘과 모발도 원형 그대로 유지하고 있었으며 일부 두피표면에 모발이 빠져서 형성된 모공들이 다수 관찰되었다. 채취한 두피의 횡단면 두께는 약 1mm로 나타났으며 표피와 진피 및 피하조직이 뚜렷하게 존재하였다. 투과전자현미 경상에서 상피세포의 구조나 형태는 관찰되지 않았으며 또한, 세포의 형태를 그대로 유지하고 있는 어떠한 세포들도 전혀 관찰할 수 없었다. 그러나 진피의 교원섬유들은 잘 보존되어 있었고 이들 사이에 Clostridium 속으로 추정되는 부패세균들이 다수 관찰되었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권6호
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• pp.492-495
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• 2005

The role of cultured bone marrow stromal cells (BMSCs) in peripheral nerve regeneration was examined using an established rabbit peroneal nerve regeneration model. A 15-mm peroneal nerve defect was bridged with a vein filled with BMSCs $(1{\times}10^6)$, which had been embedded in collagen gel. On the contralateral side, the defect was bridged with a vein filled with collagen gel alone. When the regenerated tissue was examined 4, 8 and 12 weeks after grafting, the number and diameter of the myelinated fibers in the side with the BMSCs were significantly higher than in the control side without the BMSCs. This demonstrates the potential of using cultured BMSCs in peripheral nerve regeneration.

• 대한임상검사과학회지
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• 제37권1호
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• pp.35-40
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• 2005

This paper reports the morphological nature of the remodelled interface process between implants and surrounding bone after 1, 4, 6, 8 and 12 weeks of implantation of smooth machined implants into rat tibias. After 4 weeks of implantation, histochemical analysis showed that the new bone was growing in direct contact with the implant. In the forming process, the activatived osteoblast cells migrated toward the interface and colonized the surface at the contact areas. This immature woven bone, rich in osteocyte lacunae, was deposited directly onto the implant surface. Osteoblast activity was found to continue ill 12 weeks of implantation The osteoblasts in lacunar areas developed numerous processes and synthesized bone matrix, after all, surrounded by secreting matrix. At the 12th week, the amount of newly formed bone matrix between bone and implant increased in mineralization. The mineralized mature bone contained well organized collagen fibers with characteristic banding pattern bone tissue formation around the implant.

• Animal cells and systems
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• 제11권2호
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• pp.155-160
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• 2007

The skin of the Korean bullheads, Pseudobagrus brevicorpus and P. koreanus, is composed of epidermis, dermis and subcutis. The epidermis has three layers, the outermost layer, middle layer and stratum germinativum. The epidermis consists of two types of gland cells, an unicellular mucous cell of sulfomucin and a large club cell having sometimes two nuclei. The epidermis has numerous intraepithelial blood vessels in P. brevicorpus but not at all in P. koreanus. Lymphatic spaces containing lymphocytes are well developed in mainly the stratum germinativum. The dermis lacks scales and consists mostly of bundles of coarse collagen fibers. The collagen bundles are arranged in parallel to each other in the dorsum and lateral region toward the dorsum, but vertically at intervals in the abdomen and lateral region toward the abdomen. Considering this unique skin structure, the two species are likely to exercise cutaneous respiration as a dual respiratory system to overcome hypoxic conditions which frequently occurs in their habitats.

• 대한치과교정학회지
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• 제26권4호
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• pp.455-467
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• 1996

견인력에 의한 치아 이동시 전반적인 치주 조직의 변화를 관찰하고 특히 제1형 교원질의 발현 정도 및 분포의 변화를 알아보고자, Sprague-Dawley계 백서 21마리를 대상으로 대조군(3마리)과 실험군(18마리)으로 나누었으며, 실험군은 양 중절치 사이에 견인력(75g)을 가한 후 12시간, 1일, 4일, 7일, 14일, 28일째에 각각 3마리씩 희생시켜, 시간에 따른 제1형 교원질의 발현과 조직학적 변화를 면역조직화학적 및 조직병리학적으로 관찰한 바 다음과 같은 결과를 얻었다. 견인력을 가한 후 28일째까지, 견인측의 치주인대 섬유는 신장되어 있었고, 압박측의 치주인대 섬유는 압축되어 있었으며 치주인대 섬유의 배열은 완전히 회복되지 않았다. 대조군에서의 제1형 교원질 발현은 구강 상피, 전상아질, 치수와 치주인대에서 경미하였으나, 치조골에 연한 조골세포, 치근단의 무세포성 백악질, 조백악세포와 악간봉합 부위에서 약양성의 발현을 보였다. 실험군에서의 제1형 교원질의 발현은, 무세포성 백악질에서 치아 이동 1일째에 중등도, 7일째부터 강양성의 발현을 보였으며, 정중 구개 봉합 부위에서는 치아 이동 1일째에 중등도, 14일째부터 강양성 발현을 보였다. 치주인대에서는 치아 이동 4일째에서 견인측이 압박측보다 제1형 교원질의 발현이 많아져 7일째에서 최고조에 달하다가 14일째부터는 측간의 차이가 없었다. 골조직에서는 조골세포가 붙어 있는 골 주변부에서 강하게 반응을 보여 대조군과 구별되었으며 특히 7일째 이후에 많은 것으로 관찰되어 골개조 현상과 관련하여 주목되었다. 이러한 관찰로 교정적 치아 이동 후 골재형성 과정은 조직 형성 세포의 분화와 이들 세포에서 형성되는 제1형 교원질과 밀접한 관계가 있음을 확인할 수 있었다.