• 한국동물생명공학회지
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• 제36권4호
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• pp.253-260
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• 2021

Organ transplantation is currently the most fundamental treatment for organ failure, but there is a shortage of organ supply compared to those in need. Regenerative medicine has recently developed a decellularization technique that overcomes the limitations of conventional organ transplantation and attempts to reconstruct damaged tissues or organs to their normal state. Several decellularization methods have been suggested. In this experiment, the decellularization methods were used to find effective decellularization methods for humanlike porcine placenta. The optimal conditions for decellular support are low DNA content and high glycos amino glycans (GAGs) and collagen content. In order to satisfy this condition, SDS and Triton X-100 and SDS + Triton X-100 were used as the detergent used for decellularization in this experiment. The contents were compared according to the decellularization time (0, 12, 24, 48 and 72 hours), and the concentrations of SDS (0.2, 0.5, 0.7 and 1.0%) were mixed in 1.0% Triton X-100 to analyze the contents. When decellularized using SDS and Triton X-100, respectively, it was confirmed that the contents of DNA and GAGs were opposite to each other. And decellularization treatment for 24 hours at 0.5% SDS was able to obtain an effective decellular support. If decellularization studies of various detergents can be obtained an effective decellular support, and furthermore, cell culture experiments can confirm the effect on the cells.

• 한국가금학회지
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• 제29권1호
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• pp.51-57
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• 2002

본 연구는 국내 유통 닭고기의 육질 특성을 구명하기 위한 기초자료를 수집하기 위하여, 일일 도계량이 50,000수 이상 되는 대형 도계장에서 구입한 닭고기의 부분육에 대한 화학적 분석을 실시하였다. 일반성분의 수분함량은 봉과 넓적다리에서 각각 73.37, 73.19%를 나타낸 반면 북채와 날개는 75.65, 75.76%를 나타내었다. 조단백질은 가슴살 및 안심이 23.17, 22.96%으로 비교적 높은 반면 북채와 넓적다리에서 18.74, 18.77%를 나타내어 가슴살이 비교적 높은 단백질 함량을 나타내었다. 반면에 조지방은 가슴살 및 안심의 평균이 0.73%를 나타낸 반면, 넓적다리에서는 6.86%를 나타내 가슴살 부위가 타 부위에 비해 낮은 지방 함량을 나타내었다. 무기물 함량에서 Fe는 안심에서 4.0ppm을 나타낸 반면, 넓적다리에서 7.3ppm으로 1.8배이상 높은 것으로 나타났다. Zn도 안심에서 6.0ppm을 나타낸 반면, 봉에서는 15.0ppm으로 2배 이상의 함량을 나타내었다. 아미노산 중에서 맛과 관련이 있는 glutamic acid의 함량은 안심에서 4.02%으로 북채의 2.42%보다 1.7배정도 높게 나타났고, methionine의 함량은 안심이 0.56%, 북채가 0.49%으로 안심이 약간 많이 함유된 경향을 나타내었다. 콜라겐함량에서 북채는 3.97mg/g과 넓적다리 3.24mg/g으로 가슴부위보다 많은 함량을 나타냈다. 날개부위의 콜라겐 함량은 윙이 2.64mg/g와 봉은 3.70mg/g을 함유하고 있었다.

• 유기물자원화
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• 제5권2호
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• pp.47-56
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• 1997

피혁 제조시 발생되는 크롬을 함유한 피혁 고형폐기물인 shaving scraps의 단백질 자원화 가능성을 검토하기 위하여 알카리제로 MgO를 농도별로 처리하여 가수분해시켜 회수한 hydrolyzed protein의 특성은 다음과 같다. MgO 처리에 의한 shaving scrap의 알카리 가수분해방법에서 크롬이 불용화 되는 pH 8.0 이상을 유지하기 위해서는 MgO를 5% 이상 처리해야 하는 것으로 나타났으며, MgO 처리에 따른 alkaline condition하에서 shaving scrap의 용해도는 약 60% 수준으로서 낮았다. MgO 처리에 의해 shaving scraps으로 부터 회수한 hydrolyzed protein의 평균 분자량은 약 80~100 KD이었고, collagen protein을 구성하고 있는 glycine, alanine 및 proline이 대부분을 차지하고 있었으며, 산성 아미노산인 aspartic acid와 glutamic acid도 상당량 함유되어 있는 것으로 나타났다. 무기성분중 Mg, Ca 및 Na는 액체비료의 무기양분으로서는 다소 많은 양이 함유되어 있었으며, Cr은 약 30~40ppm으로서 원시료 (40,000~42,000ppm)에 비해 크게 감소되었다.

• 한국축산식품학회지
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• 제32권5호
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• pp.591-597
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• 2012

This study was conducted to compare meat quality traits related to the tenderness of longissimus muscles obtained from domestic and imported steers. A total of 12 steers from three breeds were slaughtered, and were graded as quality grade 2. They were composed of 4 Hanwoo and 4 Holstein steers (domestic) as well as 4 Angus steers (imported from Australia and gained for six months in Korea until slaughtered). The longissimus lumborum muscles were separated and were stored at $4^{\circ}C$ for 7 and 14 d. Sarcomere length of Hanwoo was significantly shorter than Holstein and Angus at storage day 14 (p<0.05). The myofibrillar index was significantly lower on Hanwoo than Angus at ageing day 7, and was significantly lower than Holstein and Angus steers at storage day 14 (p<0.05). Total collagen contents of Hanwoo and Angus steers were significantly higher than Holstein on storage day 7 (p<0.05), whereas soluble collagen contents of Holstein were significantly higher than Hanwoo and Angus on storage days 7 and 14 (p<0.05). There was no significant difference in the fatty acid composition of the three breeds (p>0.05). Glutamic acid contents of Hanwoo and Angus steers were higher than those of Holstein steers at ageing day 7 and 14 (p<0.05). The results of this study have shown that there were no dramatic differences between beef from the three breeds that were fattened for 6 months under equal conditions.

• 한국축산식품학회지
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• 제27권4호
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• pp.463-468
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• 2007

본 연구는 시중에 유통중인 한우 사골의 과학적인 거래기준을 제시하고자 성숙도 2(수소), 성숙도 2(거세), 성숙도 3-9(암소)의 한우 반도체 각 4두에서 사골(상완골, 전완골, 대퇴골, 하퇴골)을 구입하여 총 4회 추출한 사골 용출액의 탁도와 색도(명도), 콜라겐과 단백질 함량, 칼로리 및 콘드로이친 황산 함량을 분석하였다. 성숙도 2에 해당하는 한우 수소와 거세우 사골 용출액의 탁도와 명도가 성숙도 3부터 9에 해당하는 한우 암소 사골용출액 보다 유의적으로 높게 나타났다(p<0.05). 추출횟수에 따른 탁도와 명도의 변화는 전체 성숙도에서 2차 추출까지 증가하다가 이후 유의적으로 감소하였다(p<0.05). 성숙도 2에 해당하는 한우 수소와 거세우 사골 용출액의 콜라겐과 단백질 함량이 가장 높게 나타났다(p<0.05). 성숙도 2에 해당하는 한우 수소와 거세우 사골 용출액의 칼로리와 콘드로이친 황산 함량이 성숙도 3부터 9에 해당하는 한우 암소 사골용출액 보다 유의적으로 높게 나타났다(p<0.05). 또한 추출횟수에 따른 콘드로이친 황산 함량 변화는 전체 성숙도에서 추출횟수가 증가 할수록 유의적으로 감소하였다(p<0.05). 이상의 결과를 종합하면, 전체 실험항목에서 성숙도 2(수소와 거세우) 사골용출액과 성숙도 3-9(암소) 사골용출액 간에는 차이가 있었으나, 전체 성숙도에서의 차이는 뚜렷하게 나타나지 않아 성숙도가 사골거래기준으로 적합한지는 보완적인 연구가 추가적으로 수행되어야 할 것으로 사료된다.

• Journal of Animal Science and Technology
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• 제47권3호
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• pp.429-438
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• 2005

This experiment was conducted to determine the effects of feeding CLA and coumarin on the changes of biochemical composition of velvet antler and blood serum in spotted deer (Cervus nippon). There were no significant differences between treatments in crude protein, crude fiber, and crude ash contents of velvet antler. The content of fatty acid did not differ between treatments, although there were significant differences (P<0.05) in C16:0 and C20:0. The content of collagen was significantly higher (P<0.05) in control than those in CLA and coumarin treatments. There were no significant differences between treatments in the contents of amino acids. Some constituents of blood showed much differences in comparison of pre and post-experiment for all treatments. Feeding of CLA and coumarin was not influential on the chemical composition of velvet antler and blood serum.

• Asian-Australasian Journal of Animal Sciences
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• 제11권6호
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• pp.648-654
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• 1998

Twenty-four Javanese thin-tail ewes (11, 9, and 4 ewes giving birth to 1, 2, and 3 lambs, respectively) with similar body weight and age at breeding were used to study serum progesterone concentrations during pregnancy, milk production during lactation, and mammary gland indices at the end of lactation (3 months postpartum). The results of the experiment showed that averages serum progesterone concentrations during pregnancy in the ewes giving birth to twin and triplet lambs were higher (p < 0.01) than those giving birth to a single lamb. Ewes giving birth to 3 lambs had higher (p < 0.01) mammary dry fat-free tissue (DFFT) (by 31 and 34%), DNA concentration (by 25 and 16%) and RNA concentration (by 29 and 16%) at the end of lactation than those giving birth to 1 and 2 lambs. There was no difference in mammary collagen, protein and glycogen concentrations at the end of lactation among litter sizes. Ewes giving birth to 3 lambs had higher (p < 0.01) total mammary DNA content (by 64 and 61%) and RNA content (by 69 and 53%) at the end of lactation than those giving birth to 1 and 2 lambs. There was no difference in total mammary collagen, protein and glycogen contents at the end of lactation among litter sizes. Even though ewes with higher litter size had numerically higher milk production, there was no significant difference in milk production per 4 h among litter sizes. The results of the experiment indicated that ewes having higher litter size had greater mammary cell number and synthetic activities at the end of lactation. The results suggested that ewes with higher progesterone concentrations and better developed mammary glands during pregnancy could maintain higher cell number and activities throughout lactation.

• 대한한의학회지
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• 제32권4호
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• pp.83-99
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• 2011

Objective: This study aimed to evaluate the protective effects of Socheongryong-tang (SCRT) on elastase-induced lung injury. Materials and Methods: The extract of SCRT was treated to A549 cells and elastase-induced COPD mice model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed. Results: SCRT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. SCRT treatment also revealed the protective effect on elastase-induced COPD mice model. This effect was evidenced via histopathological findings including immunofluoresence stains against elastin, collagen, and caspase 3, and protein level of Cdc2, cyclin B1, and phospho-Erk1/2 in lung tissue. Conclusion: These data suggest that SCRT has pharmaceutical properties on COPD. This study provides scientific evidence for the efficacy of SCRT for clinical application to patients with COPD.

• 대한한의학회지
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• 제32권2호
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• pp.63-78
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• 2011

Objective: This study aimed to evaluate the protective effects of Maekmundong-tang (MMDT) on an elastase-induced COPD model. Materials and Methods: The extract of MMDT was treated to A549 cells and elastase-induced COPD mice model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed. Results: MMDT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, protein level of Cdk1, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. MMDT treatment also revealed a protective effect on the elastase-induced COPD mice model. This effect was evidenced via histopathological finding including immunofluorescence stains against elastin, collagen, and caspase 3, and protein level of Cdc2 in lung tissue. Conclusion: These data suggest that MMDT has pharmaceutical properties on COPD. This study can provide scientific evidence for the efficacy of MMDT for clinical application to patients with COPD.

• Biomolecules & Therapeutics
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• 제12권3호
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• pp.157-164
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• 2004

DA-3711 is a novel anti-wrinkle agent containing growth factors derived from culture medium of artificial human skin. Photoprotective effect by DA-3711 against chronic UVB (ultraviolet B)-induced skin damage was investigated in hairless mice model. Methods: After hairless mice were irradiated to induce photodamage for 8 weeks with UVB, grouped mice were treated topically once a day with lotion base, DA-3711 (30% or l5%), Cylasphere retinol$^{\circed{R}}$ (2500 I.U.), NouriCel$^{\circed{R}}$ along with concomitant exposure to UVB for further 8 weeks. Then mice were sacrificed to assess photodamage-protective effect by replica analysis, biochemistry and histology. DA-3711 of 30% lotion significantly reduced UVB radiation-induced wrinkling, histological alterations and increased collagen contents. Whereas DA-3711 of l5% lotion and NouriCel$^{\circed{R}}$ treatment showed a partial protective effect on skin wrinkle, epidermal and dermal thickness, and collagen content, Cylasphere retinol$^{\circed{R}}$ showed no protective effects. These results demonstrate that topical application of DA-3711 can alleviate UVB-induced photodamage and potentially be used for reduction of UVB-induced photodamage.