• Title/Summary/Keyword: cold preservation

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Comparative Study of Prolonged Preservation Methods in Rabbit Heart for Transplantation (이식을 위한 가토심장의 장기 보존방법에 관한 비교 연구)

  • Jo, Hyeong-Gon;Kim, Su-Hyeon;Kim, Song-Myeong
    • Journal of Chest Surgery
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    • v.30 no.1
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    • pp.1-10
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    • 1997
  • The successful cardiac transplantation depends partly on the donor heart preservation by a solution that will ensure recovery of myocardial function. The purpose of this study was to perform the evaluation of various preservation solutions and to accumulate the data on the requisites for ideal preservation solution. The experimental setup was the constant pressure Langendorffs perfusion system. Isolated rabbit hearts were perfused for 20minutes with unarm Krebs-Henseleit solution, stored for 4 hours in cold preservation solution after cardioplegia, and then were reperfused for 20minutes. The 4 experimental groups were prepared Hartmann's solution group (group 1, control), modified Euro-collins solution group(group II. MEC), modified University of Wisconsin group (group n, MUW), and CK solution(made by the author) group (group W, CK). The parameters for assessing the preservation ability were levels of enzymes in freezed myocardial tissues (lactate, creatine kinase-MB and adenosine deaminase), coronary flow. left ventricular developing pressure and dpldt. In conclusion, the ability of preservation for isolated rabbit heart was excellent in CK solution and modified University of Wisconsin solution, and poor in modified Euro-collins solution, compared with Hartmann solution. CK solution has low potassium concentrations(34.2mEq/L) and includes various substrates to be salutary on myocardial preservation. This fact may indicates the necessity of further refinements in selection or composition of electrolytes and substrates.

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Physico-chemical Properties and Cold Storage of River Puffer (Takifugu obscurus) Milt (황복(Takifugu obscurus) 정액의 물리$\cdot$화학적 성상과 냉장보존)

  • CHANG Young Jin;LIM Han Kyu;CHANG Yun Jeong;KIM Hyung Sun;HUH Hyung Tack
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.3
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    • pp.243-246
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    • 1999
  • To obtain the basic data for the preservation of river puffer (Takifugu obscurus) sperm, experiments were carried out on the physico-chemical properties and cold storage of milt. The average number of sperm and spermatocrit in milt stripped were $1.13\pm0.34\times10^{10}/ml$ and 64.8$\pm$1.4, respectively. Osmolality of seminal fluid was 266$\pm$2 mOsm/kg, Total protein and total lipid from sperm were higer than that from seminal fluid. $Ca^{2+}$ and $Na^{+}$ concentrations were higher in the seminal fluid than in the sperm, while $Mg^{2+}$ and $K^{+}$ concentrations were lower in the seminal fluid. When sperm of river puffer were preserved in $0\pm0.5^{\circ}C$ with various diluents for 16 days, fertilization rate was $0\~0.7\%$. It suggested that cold storage of river puffer sperm was detrimental to sperm fertility.

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A Study on Green Space Management Planning Considering Urban Thermal Environment (도시 열환경을 고려한 녹지관리방안 수립 연구)

  • Joo, Chang-Hun;Kim, Jeong-Ho;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.23 no.7
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    • pp.1349-1358
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    • 2014
  • This study suggests plan of green space management based on the result of research apprehending the characteristic through sorting types of city thermal environment targeting summer which thermal pollution is the most serious. Considering anthropogenic heat, development level of wind road, thermal environment, as a result of types of thermal environment process, it is appeared 36 types, and 10 types is relevant of this research subject. Type I-1, size of building is large, artificial covering area is wide, and thermal load of anthropogenic heat is high, type II-1, development condition of wind road is incomplete as IIlevel, entering cold air is difficult and thermal management and improvement is needed area. Type III-1, scale is large and it is area of origin of cold air, development level of wind road is mostly favorable, type III-2 is revealed as smaller scale than III-1, and small area of origin of cold air. Type IV, anthropogenic heat is $81{\sim}150W/m^2$, average, but development function of wind road is very favorable. Type V, large area of thermal load and the origin of cold air are distributed as similar ratio, and level of development function of wind road is revealed as II level. According to standard of type classification of thermal environment, as a result of suggesting plan of green space management and biotops area ratio, type I-1 is buffer green space and waterway creation, goal biotops area ratio 35%, type II-1 afforestation in site and goal biotops area ratio 40%, type III-1, preservation plan to display the current function continuously is requested. Type IV suggests afforestation of stream current, and type V suggests quantitative increase of green space and goal biotops area ratio 45%.

Management Planning of Wind Corridor based on Mountain for Improving Urban Climate Environment - A Case Study of the Nakdong Jeongmaek - (도시환경개선을 위한 산림 기반 바람길 관리 계획 - 낙동정맥을 사례로 -)

  • Uk-Je SUNG;Jeong-Min SON;Jeong-Hee EUM;Jin-Kyu MIN
    • Journal of the Korean Association of Geographic Information Studies
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    • v.26 no.1
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    • pp.21-40
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    • 2023
  • This study analyzed the cold air characteristics of the Nakdong Jeongmaek, which is advantageous for the formation of cold air that can flow into the city, in order to suggest the wind ventilation corridor plans, which have recently been increasing interest as a way to improve the urban thermal environment. In addition, based on the watershed analysis, specific cold-air watershed areas were established and management plans were suggested to expand the cold air function of the Nakdong Jeongmaek. As a result of the analysis of cold air in the Nakdong Jeongaek, cold air was strongly generated in the northern forest of the Jeongamek, and flowed into nearby cities along the valley topography. On average, the speed of cold air was high in cities located to the east of the Jeongmaek, while the height of cold air layer was high in cities located to the west. By synthesizing these cold air characteristics and watershed analysis results, the cold-air watershed area was classified into 8 zones, And the plans were proposed to preserve and strengthen the temperature reduction of the Jeongmaek by designating the zones as 'Conservation area of Cold-air', 'Management area of Cold-air', and 'Intensive management area of Cold-air'. In addition, in order to verify the temperature reduction of cold air, the effect of night temperature reduction effect was compared with the cold air analysis using weather observation data. As a result, the temperature reduction of cold air was confirmed because the night temperature reduction was large at the observation station with strong cold air characteristics. This study is expected to be used as basic data in establishing a systematic preservation and management plan to expand the cold air function of the Nakdong Jeongmaek.

Fatty Acid Composition of Concentrated Phytoplanktons by Cold Storage and Their Effects on the Larval Survival of Pacific Oyster, Crassostrea gigas (냉장보관된 농축 먹이생물의 지방산 조성 변화와 농축먹이로 사육한 굴 유생의 생존율)

  • LIM Hyun Jeong;PARK Sung Real
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.4
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    • pp.567-573
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    • 1998
  • Cold storage of concentrated food phytoplanktons is a useful technique in supplying food organisms for artificial shellfish seed. One month after preservation at $4^{\circ}C$, we have measured survival rate of the concentrated food phytoplanktons, Pavlova lutheli, Isochrysis galbana, Isochrysis aff, galbana and Chaetoceros calcitrans. Thereafter we determined survival rate of oyster lavae fed fresh and concentrated diets and fatty acid compositions of the fresh and concentrated food phytoplanktons. Survival rate of concentrated planktons ranged from $23\%$ to $31\%$ after one month at $4^{\circ}C$. The survival rate of oyster larvae fed cold stored food appeared generally higher than those fed fresh harvested food. Especially, the highest survival rate were found in the larvae fed cold stored concentrated I. aff. galbana. EPA and DHA increased after cold storage and the highest level of DHA was detected in I. aff. galbana. As DHA can role as an important factor in determing nutritional value, it would be better to use concentrated I aff, gaibana kept in cold refrigerator for oyster seed production.

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Preservation Experiment of Hibernating Silkworm Eggs by 2 Year Cold Refrigeration (월년잠종의 2년간 냉장에 의한 보존 시험)

  • Sohn, Bong-Hee;Kang, Pil-Don;Lee, Sang-Uk;Kim, Yong-Soon
    • Journal of Sericultural and Entomological Science
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    • v.46 no.2
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    • pp.65-71
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    • 2004
  • Long term preservation experiment through refrigeration was conducted for 2 year on 300 lines of silkworm races preserved, as one method for the development of long term safe preservation technique. Experiment with 6 treatments was conducted for 680 days from July 1 st 2000 to May 1st 2002. Embryonic development was conducted to each treatment. There are no differences among treatments and races in 400 days preservation, the stage of whole embryonic development was Eul B and condition of eggs was good. In 650 days preservation experiment, differences were reveled among races and treatment, the level of whole embryonic development was Byeong A and condition of eggs was good. The order of embryonic development is European races >Tropical, Korean races >Japanese, Chinese races, thus European races showed fast embryonic development. Control(treatment A) and treatment C showed faster development than other treatments. And treatment D and F showed stable individual stage among all treatments. The test of shape characteristics and embryo which were conducted in hatching period showed 61% of high line succession possibility in average of 6 treatments. But treatment A and B showed no hatching, 3 lines of treatment C, 48 lines of treatment D, 1 line of treatment E, and 29 lines of treatment F showed slow development. And treatments D and F which showed stable embryo condition had highest possibility. The two treatments D and F showed good result among six treatments, and the preservation period of treatment D and F are 235 days and 310 days, and exposure period in $-2.5^{\circ}C$...was longer than other treatments. Numbers of hatched lines of treatment D and F are 48 and 29, and occupied 15.6% and 9.4% of all tested lines, respectively. Average hatching ratio of treatment D and F were 54.% and 71.6%, and average dead egg ratio were 12.6% and 2.4%, respectively. These results show that average ratio of hatching dead eggs in treatment D and F are higher than general line. Thus reconsideration of hatching condition on treatment D and F is needed.

Effect of Selenium Treatment on the Quality of 'Wonwhang' Pear Fruit (셀레늄 처리가 배 '원황' 과실 품질 특성에 미치는 영향)

  • Kim, Hyun-Ji;Kim, Wol-Soo;Choi, Hyun-Sug
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.838-842
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    • 2009
  • We explored how 'Wonwhang' pear fruit quality was affected by selenium (Se) application. The fruit skin color and fruit type differed between Se-treated trees and controls. Se-treated fruits had higher potassium and Se concentrations, more fruit soluble solids, and lower acidity, compared to the controls. All Se treatments improved fruit firmness during cold temperature storage compared to the controls. Also, Se-treated fruits had greater specific gravity and minuteness, which reduced fruit pithiness and rendered test fruits denser than the controls. Overall, Se foliar application improved fruits marketability compared to the Se fertilization or Se trunk injection.

The Medicinal Effects of Seafoods in ${\ulcorner}$Lim-Won-Sib-Rheuk-Ji${\lrcorner}$ ("임원십육지(林園十六誌)"에 수록된 어패류의 향약성(鄕藥性)에 관한 연구)

  • 최영진;백숙은
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.3
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    • pp.187-195
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    • 2004
  • This study was conducted to figure out the nutritional nature of seafoods categorized according to the medicinal effects and the usages introduced in Lim-Won-Sib-Rheuk.-Ji, one of the well-known encyclopedias published during the Chosun Dynasty. They were compared with those introduced in other cooking books in order to find out how they were used in the diet of real-life. The seafoods of the book were first categorized into the fish 20, the mollusk 5, the crustacea 4, and the shellfish 7, and reptile according to the biological taxonomy, and then each one was reviewed in terms of the taste and the food-nature. Generally, the fishes had the sweet taste; the crustacea and the shellfish the salty or sour in addition to the sweet taste; none of them were bitter or hot. Most of them had the mild nature; seven of them were regarded as warm; four as cool; one as cold from the standpoint of the traditional medicine. The food-nature of seafoods was closely related with the medicinal effects on the human body. These seafoods were characteristically used in daily dietary life as well as the medical applications; used as the side-dishes rather than the main-dishes. The cooking methods of boiling, steaming, and baking were preferred to drying and stewing. The preservation methods favored were the fermentation, drying and salting. These cooking and preservation methods were believed to reflect the nature of the seafoods to manifest the medicinal effects.

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Brain Death and Kidney Transplantation in Dogs (개의 뇌사와 신장이식)

  • 우흥명;권오경
    • Journal of Veterinary Clinics
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    • v.18 no.4
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    • pp.358-362
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    • 2001
  • Brain dead (BD) patients remain the largest source of solid organs for transplantation. BD has shown to decrease graft function and survival in rodent models. The aim of this study was to evaluate how brain death affects graft viability in the donor and kidney tolerance to cold preservation as assessed by survival in a canine transplantation. 13 Beagle dogs were used for the study. Brain death was induced by the sudden inflation of a subdural balloon catheter with continuous monitoring of arterial blood pressure and eletroencephalographic activity (n=3). Sixteen hours after conformation of brain death, kidney graft were retrieved (n=6). Non-BD donors served as controls (n=4). All kidneys were flushed with University of Wisconsin (UW) solution and preserved for 24 hours at 4$^{\circ}C$ before transplantation. Recipient survival rates, serum creatinine level were analyzed. Brain death induced the well-known Cushing reaction with a severe increase in blood pressure and tachycardia. Thereafter, cardiac function returned progressively to baseline within 8 hours and remained stable until the end of the experiment. All of dogs in both group transplanted were survived until 7 days (100%), and the kidneys showed functional early rejection at 8.3$\pm$0.5 days and 8.5$\pm$0.5 days after transplantation, in BD and allograft group, respectively. BD kidneys were functionally similar to control kidneys for 7 days after transplantated. Brain death has no deleterious effect on preservation injury and survival of dog kidney transplantation, although it induces changes in hemodynamic parameters. This study reveals that kidneys from BD donors do not exhibit more ischemia reperfusion injury, and support good early function and survival.

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Studies on the production and marketing of apple of Kyungpook region and strategies of its improvement (경북지역의 사과생산 및 유통에 관한 연구)

  • 류진춘
    • Food Science and Preservation
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    • v.3 no.1
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    • pp.61-75
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    • 1996
  • Apple is most favorable fruit in Korea, and apple farmland has been increased before and after the agreements of Uruguay Round and apple is considered as one of strategic agricultural products. Especially expansion of apple farmland is concentrated in Kyungpook region because of the suitable climate and its market share is about 70 percents in 1992. The marketing channels of apples of Kyungpook region are widely classified by merchant, agricultural or horticultural co-op and large scale farmer's. Among them market share of merchant's occupy over 65 percent. In marketing margins, commercial profit is higher than cost in total marketing margins and, assembler and retailer's margin is not less than wholesaler's. The fluctuation of the price of apple is high in year. The marketing problems of apples are several, first, complex marketing channels, secondly, the high percentage of market share by growing district assembler, thirdly, low rate of package and quality standardization, finally, concentration of shipment of apple because of the shortage of apple processing, storage and marketing facilities, of newly produced apples. In conclusion, to increase apple grower's income with the stabilization of supply and quality upgrade, the improvement measures of marketing system are as follows, first, government level's support in marketing facilities and mechanism, secondly, the increment of supply by grower's cooperatives, thirdly, the establishment of a serious of marketing system to increase the efficiency, fourthly, the establishment of cold-chain system and quality standardization of apple, finally, production of various kinds of apple processing goods.

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