• Korean Journal of Microbiology
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• v.54 no.3
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• pp.200-207
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• 2018

At high salt concentration, glycine betaine is transported into Bacillus subtilis and growing rate of the cell is not suppressed. Also according to recent studies, cell growth is maintained normal growth rate at low temperature. Low temperature results in a stress response of Bacillus subtilis that is characterized by strong repression of major metabolic activities such as translation machinery and membrane transport. In this regards, genes showing cold sensitive phenotype are cold-induced DEAD box RNA helicases (ydbR, yqfR) and fatty acid desaturases (bkdR, des). Therefore to understand the effect of glycine betaine on cold growth of Bacillus subtilis, we investigated the effect of glycine betaine on growth rate of these deletion mutants showing cold sensitive phenotype. Glycine betaine strongly stimulated growth of wild type Bacillus subtilis JH642 and deletion mutants of ydbR and yqfR at $20^{\circ}C$ (190~686 min $T_d$ difference). On the other hands, glycine betaine does not show growth promoting effects on deletion mutants of bkdR, and des at cold conditions. Same cold protectant growth results were shown with the precursor choline instead of glycine betaine. We investigated the effects of detergents on the cell membrane in bkdR and des deficient strains associated with cell membrane. It was identified that bkdR deficient strain shows retarded growth with detergent such as Triton X-100 or N-lauryl sarcosine compared with wild type cell. Thus, it is possible that deletion mutation of bkdR modifies membrane structure and effects on transport of glycine betaine.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2001.02a
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• pp.273-279
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• 2001

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2008.10a
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• pp.382-385
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• 2008

In the present study, the effect of cold reduction ratio on the spheroidization rate of SK85 high carbon steel sheet was investigated. High carbon steel sheet fabricated by POSCO was soaked at $850^{\circ}C$ for 2 hr in a box furnace and then treated at $570^{\circ}C$ and $670^{\circ}C$ for 10 min in a salt bath furnace followed by water quenching to obtain a fine pearlite structure and coarse pearlite structure. Cold rolling was conducted on the sheets by reduction ratios of 20, 30, and 40 % and heat treatment for spheroidization was carried out at $720^{\circ}C$ for the various time intervals from 1 to 32 hrs. Area fraction of spheroidized cementite was measured with an image analyzer as a function of cold reduction ratios and duration times.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.5
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• pp.670-680
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• 2022

Owing to the lack of a cold-chain distribution system, most seafood is generally distributed under room temperature conditions. However the degradation of freshness during the distribution process can lead to disputes between sellers and consumers. The most widely used method for low-temperature distribution for seafood includes packaging it with styrofoam boxes and cold packs. In this study, vacuum-packed frozen fillets of four fish species of [white meat (Paralichthys olivaceus and Sebastes schlegelii) and red meat (Scomber japonicus and Scomberomorus niphonius)] were placed in styrofoam boxes with cold packs. Thereafter, changes in chemical (including pH, volatile basic nitrogen, and trimethylamine), physical (odor intensity, hardness, and chewiness), and microbial (viable cell count) characteristics of the fillets were measured during storage at 25℃. To identify the suitable method of determining freshness during the room-temperature distribution, several factors were considered, which included significant difference verification, correlation coefficients, and economic efficiency (experimental cost and time). Volatile basic nitrogen, pH, odor intensity, and viable cell count are the most rapid and accurate freshness indicators for determining freshness of frozen fish fillets during.

• Journal of the Korea Safety Management & Science
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• v.24 no.3
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• pp.29-37
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• 2022

The development of technology related to the Fourth Industrial Revolution and the growth of the online market due to pandemic are continuing the growth of the logistics market for product delivery. If it is difficult to deliver the product directly to the customer during delivery, storage and delivery using the unmanned courier box are being carried out. However, existing storage boxes are not actively used due to lack of usability even though they have the advantage of storing goods and delivering non-face-to-face. In addition, existing courier boxes are not prepared for cold chain transportation. The unmanned delivery storage device with ICT cold chain technology should be developed to prepare for the transition to non-face-to-face society, to improve logistics efficiency and meet user's requirements. Also, it is necessary to consider the measures to reduce the safety problems that may occur during the use and maintenance of the automatic system. This study conducted a model-based analysis for the development of unmanned delivery storage devices with ICT cold chain technology, and conducted a study to derive the system development specifications that meet the requirements and secure safety and apply them to the development process.

• 한국신재생에너지학회:학술대회논문집
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• 2008.05a
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• pp.57-60
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• 2008

KEPRI has studied planar type SOFC stacks using anode-supported single cells and kW class co-generation systems for residential power generation. A 1kW class SOFC system consisted of a hot box part, a cold BOP part and a water reservoir. A hot box part contains a SOFC stack made up of 48 single cells and ferritic stainless steel interconnectors, a fuel reformer, a catalytic combustor and heat exchangers. Thermal management and insulation system were especially designed for self-sustainable operation. A cold BOP part was composed of blowers, pumps, a water trap and system control units. When a 1kW class SOFC system was operated at $750^{\circ}C$ with hydrogen after pre-treatment process, the stack power was 1.2kW at 30 A and 1.6kW at 50A. Turning off an electric furnace, the SOFC system was operated using hydrogen and city gas without any external heat source. Under self-sustainable operation conditions, the stack power was about 1.3kW with hydrogen and 1.2kW with city gas respectively. The system also recuperated heat of about 1.1kW by making hot water.

• Steel and Composite Structures
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• v.14 no.2
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• pp.105-120
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• 2013

Flexural behavior of thin walled steel-concrete composite sections as cross sections for beams is investigated by conducting an experimental study supported by applicable analytical predictions. The experimental study consists of testing up to failure, simply supported beams of effective span 1440 mm under two point loading. The test specimens consisted of composite box and channel (with lip placed on tension side and compression side) sections, the behavior of which was compared with companion empty sections. To understand the role of shear connectors in developing the composite action, some of the composite sections were provided with novel simple bar type and conventional bolt type shear connectors in the shear zone of beams. Two RCC beams having equivalent ultimate moment carrying capacities as that of composite channel and box sections were also considered in the study. The study showed that the strength to weight ratio of composite beams is much higher than RCC beams and ductility index is also more than RCC and empty beams. The analytical predictions were found to compare fairly well with the experimental results, thereby validating the applicability of rigid plastic theory to cold-formed steel concrete composite beams.

• Transactions of the Korean hydrogen and new energy society
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• v.21 no.5
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• pp.405-411
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• 2010

A 5kW class SOFC cogeneration system consisted of a hot box part, a cold BOP (balance of plant) part, and a hot water reservoir. The hot box part contained a stack, a fuel reformer, a catalytic combustor, and heat exchangers. A cold BOP part was composed of blowers, pumps, a water trap, and system control units. A 5kW stack was designed to integrate 2 sub-modules. In this paper, the 5kW class SOFC system was operated using 2 short stacks connected in parallel to test the sub-module and the system. A short stack had 15 cells with $15{\times}15 cm^2$ area. When a natural gas was used, the total power was about 1.38 kW at 120A. Because the sub-modules were connected in parallel and current was loaded using a DC load, voltages of sub-modules were same and the currents were distributed according to the resistance of sub-modules. The voltage of the first stack was 11.46 V at 61A and the voltage of the second stack was 11.49V at 59A.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.7 no.2
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• pp.43-51
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• 2001

This study was carried out to analyze changes of the physical properties of corrugated fiberboard boxes for fruits packaging by various moisture and temperature changes. There were significant decrease in burst strength with increased relative humidity. And also compressive strength(ring crush test) of corrugated fiberboard and box were reduced with moisture content. The decreasing amounts of single wall(SW) corrugated fiberboard was bigger than that of double wall(DW). The physical properties reductions of corrugated fiberboard and box were mainly affected by relative humidity. Therefore, it is suggested that development of the water-resistant corrugated fiberboard and box be useful under the condition of low temperature and high relative humidity such as the cold chain system.

• Korean Chemical Engineering Research
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• v.46 no.3
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• pp.604-609
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• 2008

This study is about the modeling and simulation of heat transfer in the box for packaging and shipping of vaccines. Comparison of the simulation results with experimental data revealed that a one-dimensional model (a spherical model of using a radius equivalent to the rectangular geometry of box) showed good agreement with experimental data during cooling process but did not successfully simulate heating process. It is considered that a rigorous boundary condition is not properly applied for outer surface of the box. However, we could successfully develop a basic algorithm for simulating heat transfer through multi-slabs combined with different materials including phase change material.