• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1179-1188
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• 2023

Escherichia coli, particularly multidrug-resistant (MDR) strains, is a serious cause of healthcare-associated infections. Development of novel antimicrobial agents or restoration of drug efficiency is required to treat MDR bacteria, and the use of natural products to solve this problem is promising. We investigated the antimicrobial activity of dried green coffee (DGC) beans, coffee pulp (CP), and arabica leaf (AL) crude extracts against 28 isolated MDR E. coli strains and restoration of ampicillin (AMP) efficiency with a combination test. DGC, CP, and AL extracts were effective against all 28 strains, with a minimum inhibitory concentration (MIC) of 12.5-50 mg/ml and minimum bactericidal concentration of 25-100 mg/ml. The CP-AMP combination was more effective than CP or AMP alone, with a fractional inhibitory concentration index value of 0.01. In the combination, the MIC of CP was 0.2 mg/ml (compared to 25 mg/ml of CP alone) and that of AMP was 0.1 mg/ml (compared to 50 mg/ml of AMP alone), or a 125-fold and 500-fold reduction, respectively, against 13-drug resistant MDR E. coli strains. Time-kill kinetics showed that the bactericidal effect of the CP-AMP combination occurred within 3 h through disruption of membrane permeability and biofilm eradication, as verified by scanning electron microscopy. This is the first report indicating that CP-AMP combination therapy could be employed to treat MDR E. coli by repurposing AMP.

• 펄프종이기술
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• 제39권2호
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• pp.45-53
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• 2007

When micro flute corrugated paperboards are used for food packaging, they necessarily need to meet the requirements for the distribution, transportation, and storage of food. The requirements could vary ac-cording to the contents in the packaging boxes. Microflute corrugated packaging paperboard for hot foods such as just-made coffee and hamburger requires to have a decent resistance property against high temperature. Along with a recent trend for small-quantity-multi-item upgraded packaging, semiconductor products and consumer-electronic appliances become to be packed using the environmental friendly micro flute corrugated paperboard. In this case, the electric resistance property of the microflute corrugated paperboard becomes important. This study was carried out to investigate on the thermal conductivity and electric resistance properties of micro flute corrugated paperboard.

• Asian-Australasian Journal of Animal Sciences
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• 제5권3호
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• pp.455-459
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• 1992

Five type of formulated diet from agricultural by-products (ABP) were fed to four breedtype of cattle in feedlot. The ABP used are palm kernel cake (PKC), palm press fibre (PPF), palm oil mill effluent (POME), cocoa pod (COP), coffee pulp (COF) and pineapple waste (PAP). The formulated diets are PS (52% PKC, 15% PPF and 30% POME), PF (57% PKC, 20% PPF and 20% POME), PA (2% PKC and 55% PAP), CO (42% PKC and 55% COP) and CF (67% PKC and 30% COF) with 1% urea, 1% NaCl and 1% vitamins premix. The cattle breedtypes are Kedah-Kelantan (KK), Brahman-KK (BK), Hereford-KK (HK) and Sahiwal-Friesian (SF). The result showed that breedtype significantly affect all the carcass characteristic except dressing percentage. Each breedtype has it's specific carcass characteristics. HK cattle gave high marbling, BK has high % of carcass bone, KK has high % of carcass meat and low % of carcass fat (lean meat type) and SF has high % of carcass fat. Diet-type significantly affect the deposition of fat in the carcass. High moisture diets (PA and CO) produced significantly higher % carcass bone, the lowest % carcass fat and the highest % carcass meat (65.3%). PF, CF, PA and CO diets produced 63.4%, 59.9%, 55.3% and 54.1% carcass meat respectively.

• 한국산학기술학회논문지
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• 제18권7호
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• pp.73-83
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• 2017

본 연구는 반추동물 사료로서 한국에서 생산된 부존사료자원 15종의 화학적 조성, 소화율 그리고 에너지 가를 평가하기 위하여 실시하였다. 반추위 그리고 십이지장 캐뉼라가 장착된 거세한우 3두(평균체중 $520{\pm}20.20kg$)를 개별 계류식우사에 공시한 후 14일의 순치기간과 3일간의 시험기간을 두었다. 실험 방법으로는 화학조성 분석법, in situ 나일론백과 모바일백 방법을 이용하였다. 사료 내 화학적 조성을 분석한 결과, 엿밥, 깻묵, 장유박 및 두유박의 조단백질 함량은 30% 이상이었다. In situ 나일론백을 이용한 사료의 분해 특성을 분석한 결과, 비트펄프, 맥주박, 커피박, 엿밥, 수수겨, 임자박, 라면박 및 두유박의 반추위 미분해 단백질 함량은 50% 이상이었다. In situ 모바일백을 이용한 사료 내 가소화 영양소총량(TDN)의 분석은 비트펄프, 맥주박, 막걸리박, 수수겨, 깻묵, 라면박, 미강, 장유박, 비지, 두유박 및 밀기울이 50% 이상인 것으로 관찰되었다. 요약하자면, 상기 부존사료자원은 배합사료 또는 조사료를 대체할 수 있는 훌륭한 사료자원으로서의 높은 잠재적 가치를 가지고 있음을 시사하고 있다. 따라서 본 연구에서 얻은 부존사료자원의 화학적 조성, 소화율 및 에너지 가를 반추동물 섬유질배합사료 제조를 위한 기초 자료로 활용하고 이를 통해 사료효율 향상, 사료비용 절감 및 환경오염 감소에 기여하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1670-1679
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• 2020

The substantial use of fungal enzymes to degrade lignocellulosic plant biomass has widely been attributed to the extensive requirement of powerful enzyme-producing fungal strains. In this study, a two-step screening procedure for finding cellulolytic fungi, involving a miniaturized culture method with shake-flask fermentation, was proposed and demonstrated. We isolated 297 fungal strains from several cellulose-containing samples found in two different locations in Thailand. By using this screening strategy, we then selected 9 fungal strains based on their potential for cellulase production. Through sequence-based identification of these fungal isolates, 4 species in 4 genera were identified: Aspergillus terreus (3 strains: AG466, AG438 and AG499), Penicillium oxalicum (4 strains: AG452, AG496, AG498 and AG559), Talaromyces siamensis (1 strain: AG548) and Trichoderma afroharzianum (1 strain: AG500). After examining their lignocellulose degradation capacity, our data showed that P. oxalicum AG452 exhibited the highest glucose yield after saccharification of pretreated sugarcane trash, cassava pulp and coffee silverskin. In addition, Ta. siamensis AG548 produced the highest glucose yield after hydrolysis of pretreated sugarcane bagasse. Our study demonstrated that the proposed two-step screening strategy can be further applied for discovering potential cellulolytic fungi isolated from various environmental samples. Meanwhile, the fungal strains isolated in this study will prove useful in the bioconversion of agricultural lignocellulosic residues into valuable biotechnological products.