• Journal of Life Science
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• v.20 no.4
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• pp.632-638
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• 2010

The genetic diversity among 48 persimmon (Diospyros kaki Thunb.) accessions, indigenous in Korea and introduced from Japan and China, was evaluated by using simple sequence repeat (SSR) markers. From 20 SSR primer sets, a total of 114 polymorphic markers were detected among 12 pollination-constant non-astringent (PCNA), 13 pollination-variant non-astringent (PVNA), 15 pollination-variant astringent (PVA), and 8 pollination-constant astringent (PCA) cultivars. Analysis of pair-wise genetic similarity coefficient (Nei-Li) and unweighted pair-group method with arithmetic averaging (UPGMA) clustering revealed two main clusters and four subclusters for cluster I. The subclustering pattern was in accordance with the classification of persimmon cultivars based on the nature of astringency loss. Phenetic relationships among the subclusters showed a closer relatedness of the PCNA group with the PVNA group, and the PVA with the PCA group. Genetic similarity co-efficiency was 0.499 on average and the highest (0.954) similarity was observed between 'Cheongdo-Bansi' and 'Haman-Bansi'. The similarity was lowest (0.192) between 'Damopan'and 'Atago'. Identification of each cultivar with the execption of 'Cheongdo-Bansi' and 'Gyeongsan-Bansi' was possible based on the SSR fingerprints, suggesting that these SSR markers are a useful tool for protecting intellectual property on newly developed cultivars.

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.105-110
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• 2015

BACKGROUND: Soy isoflavones, structurally similar to endogenous estrogens, may affect human body through both hormonally mediated and non-hormonally related mechanisms. Heat processing could change chemical compositions. The effects of different thermal processes, boiling and HTHP(high temperature and high pressure) on the composition of isoflavone compounds and total amount of domestic soybeans were investigated in this study. METHOD AND RESULTS: Three different kinds of soybean samples were collected from RDA-Genebank. The samples were extracted using methanol, distilled water, and formic acid based solvent. Also the same solvents were used for mobile phase in UPLC/ToF/MS. All of the isoflavone compounds were analyzed based on the aglycone type of external standard for quantification. The standard calibration curve presented linearity with the correlation coefficient R2 > 0.98, analysed from 1 to 50 ppm concentration. The total isoflavone contents does not change by treatment within the same breed. While "boiling" and "HTHP" processes tend to increase the contents of aglycone and ${beta}$-glucosides, "fresh" soybeans retained the high concentration of malonylglucosides. CONCLUSION: These results have to be considered while developing an effective functional food, from the health while point of view using soybeans.

• Journal of Crop Science and Biotechnology
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• v.11 no.2
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• pp.141-150
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• 2008

The response of grain yield(GY) and milled-rice protein content(PC) to crop growth status and nitrogen(N) rates at panicle initiation stage(PIS) is critical information for prescribing topdress N rate at PIS(Npi) for target GY and PC. Three split-split-plot experiments including various N treatments and rice cultivars were conducted in Experimental Farm, Seoul National University, Korea in 2003-2005. Shoot N density(SND, g N in shoot $m^{-2}$) and canopy reflectance were measured before N application at PIS, and GY, PC, and SND were measured at harvest. Data from the first two years(2003-2004) were used for calibrating the predictive models for GY, PC, and SND accumulated from PIS to harvest using SND at PIS and Npi by multiple stepwise regression. After that the calibrated models were used for calculating N requirement at PIS for each of nine plots based on the target PC of 6.8% and the values of SND at PIS that was estimated by canopy reflectance method in the 2005 experiment. The result showed that SND at PIS in combination with Npi were successful to predict GY, PC, and SND from PIS to harvest in the calibration dataset with the coefficients of determination ($R^2$) of 0.87, 0.73, and 0.82 and the relative errors in prediction(REP, %) of 5.5, 4.3, and 21.1%, respectively. In general, the calibrated model equations showed a little lower performance in calculating GY, PC, and SND in the validation dataset(data from 2005) but REP ranging from 3.3% for PC and 13.9% for SND accumulated from PIS to harvest was acceptable. Nitrogen rate prescription treatment(PRT) for the target PC of 6.8% reduced the coefficient of variation in PC from 4.6% in the fixed rate treatment(FRT, 3.6g N $m^{-2}$) to 2.4% in PRT and the average PC of PRT was 6.78%, being very close to the target PC of 6.8%. In addition, PRT increased GY by 42.1 $gm^{-2}$ while Npi increased by 0.63 $gm^{-2}$ compared to the FRT, resulting in high agronomic N-use efficiency of 68.8 kg grain from additional kg N. The high agronomic N-use efficiency might have resulted from the higher response of grain yield to the applied N in the prescribed N rate treatment because N rate was prescribed based on the crop growth and N status of each plot.

• Journal of Life Science
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• v.24 no.11
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• pp.1174-1179
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• 2014

This research was carried out to evaluate whether gamma ray is a useful tool for breeding new strains of mushrooms. For this research, 5 mutant groups, 20 strains of Hypsizigus marmoreus, 2 strains of Lyophyllum decastes, and 1 strain of Lyophyllum shimeji were used. Monokaryon spores from one variety of H. marmoreus were irradiated with 50~2,000 Gy of gamma ray. The propriety dose was 50~200 Gy for mutagenesis. Mutant monokaryon mycelia crossed each order to become dikaryon mycelia. The internal transcribed spacer (ITS) regions of rDNA were amplified using PCR, and the products were sequenced. The sequences of the ITS regions (16 partial rDNA, complete ITS1, 5.8 rDNA and partial rDNA) were analyzed by PCR, and strains of H. marmoreus, L. decastes, and L. shimeji were auto-sequenced. The lengths of the sequenced ITSs were 1,052~1,143 nucleotides. Genetic matrices were calculated using Nei-Li's genetic distance coefficient based on ITS sequence. The dissimilarities were 0~3.35% in strains of H. Hypsizigus. In addition, a phylogenetic tree was constructed based on ITS sequences using the neighbor-joining (NJ) method. The phylogenetic tree revealed that 23 strains and 5 mutant groups were divided into 12 clusters; the mutant groups fell into different clusters. These results show that mushroom spores were mutated effectively by gamma ray; therefore, gamma ray could be a useful tool for breeding new strains of mushrooms.

• Korean Journal of Remote Sensing
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• v.33 no.6_1
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• pp.961-970
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• 2017

Despite of large demand for high spatial resolution products of aerosol properties from satellite remote sensing, it has been very difficult due to the weak signal by a single pixel and higher noise from clouds. In this study, aerosol retrieval algorithm with the high spatial resolution ($500m{\times}500m$) was developed using Geostationary Ocean Color Imager (GOCI) data during the Korea-US Air Quality (KORUS-AQ) period in May-June, 2016.Currently, conventional GOCI Yonsei aerosol retrieval(YAER) algorithm provides $6km{\times}6km$ spatial resolution product. The algorithm was tested for its best possible resolution of 500 m product based on GOCI YAER version 2 algorithm. With the new additional cloud masking, aerosol optical depth (AOD) is retrieved using the inversion method, aerosol model, and lookup table as in the GOCI YAER algorithm. In some cases, 500 m AOD shows consistent horizontal distribution and magnitude of AOD compared to the 6 km AOD. However, the 500 m AOD has more retrieved pixels than 6 km AOD because of its higher spatial resolution. As a result, the 500 m AOD exists around small clouds and shows finer features of AOD. To validate the accuracy of 500 m AOD, we used dataset from ground-based Aerosol Robotic Network (AERONET) sunphotometer over Korea. Even with the spatial resolution of 500 m, 500 m AOD shows the correlation coefficient of 0.76 against AERONET, and the ratio within Expected Error (EE) of 51.1%, which are comparable to the results of 6 km AOD.

• Journal of Wetlands Research
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• v.13 no.3
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• pp.633-641
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• 2011

The Philippines is known for its abundant water resources such as the rainfall, where it has a mean annual rainfall range from 965 to 4,100mm. Due to the rapid urbanization of the country, the population in Metro Manila has been continuously increasing hence, the demand for a potable water supply also increases. To mitigate the scarcity of potable water supply, utilization of the water resources should be practiced. Rainwater harvesting is one way to utilize the rainfall runoff. This study analyzedthe potentiality of the rainwater harvesting on residential areas in Metro Manila. A water balance method based spreadsheet was used with input parameters including daily rainfall, catchment area, runoff coefficient, population and the water demand. The efficiency of the domestic water tank was analyzedusing the three different climatic conditions (i.e., minimum, median andmaximum annual rainfalls) and three different types of toilets (i.e., inefficient, conventional and dual-flush toilets). Furthermore, the overflow volume was used to determine which size of rainwater storage was more appropriate for the study area. The results of the study showed that for the three types of rainfall years, only the conventional and dual-flush toilets were suitable for the utilization of rainwater harvesting. The utilization of the $60m^3$ storage tank was sufficient for supplying the demandsof the 90 houses only for a small period of time, 3 months. Based from this study, to fully sustain the long-term water demand of the houses, the enlargement of the tank size having a capacity of 1,100 to $2,500m^3$ is ideal.

• Journal of Korea Water Resources Association
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• v.44 no.2
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• pp.157-167
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• 2011

A numerical model was developed to predict the stage-discharge curve and lateral distribution of unit discharge in open channels with nonuniform cross section or compound open-channels. The governing equation is the one-dimensional momentum equation based on assumptions of the steady and uniform flow conditions in the longitudinal direction and the uniform water surface elevation in a cross section. Vegetative drag force term was included in governing equation in order to reflect the effect of floodplain vegetation on the flow characteristics. Finite element method was applied to obtain the numerical solution of the governing equation. Stage-discharge curve and lateral distribution of unit discharge for a given water surface are calculated based on input data, such as the cross sectional geometry, Manning's roughness coefficient, vegetative information and longitudinal slope of channel bed. The developed model was verified by comparing the calculated results with the observed data and the results of Darby and Thorne's(1996) model and the nonlinear k-$\epsilon$ model. The verified model was applied to estimate the upstream boundary conditions in two-dimensional flow model. The numerical results using laterally distributed unit discharge were compared with those obtained using uniformly distributed unit discharge in two-dimensional flow model.

• Journal of Korea Water Resources Association
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• v.45 no.11
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• pp.1157-1168
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• 2012

In this study, we assess changes in water quality trends over time based on certain control measurements in order to identify and analyze the cause of the trend in water quality. The current water pollution in the Nakdong River was analyzed, as it suggests that the significant changes in water quality have occurred in between 2006 and 2010. Based on monthly average data, we have examined for trends of the Nakdong River watershed in water temperature, Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Nitrogen (TN), and Total Phosphorus (TP). Moreover, we have investigated seasonal variation of water quality of sites within the Nakdong River Basin by implementing further analyses such as, Correlation Coefficient, Regression Analysis, Hierarchical Clustering Method, and Time Series Analysis on SPSS. Geology and topography of the watershed, controlled by various conditions such as, climate, vegetation, topography, soil, and rain medium, have been affected by the non-homogeneity. Our study suggests that such variables could possibly cause eutrophication problems in the river. One possible way to overcome this particular problem is to lay up a ship on the river by increasing the nasal flow measurement of the Nakdong River during rainy season. Moreover, the water management requires arranging the measurement of the flow in order to secure the river while the numerous construction projects need to be continuously observed. However, the water is not flowing tributary of the reason for the timing to be flowing in a natural state of river water and industrial water intake because agriculture. Therefore, ongoing research is needed in addition to configuration of all observations.

• Journal of Korean Home Economics Education Association
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• v.23 no.3
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• pp.121-138
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• 2011

This research examined the amounts and methods change of recommended energy intake(REI) from 1962's recommended dietary intakes for Korean to 2010's dietary reference intakes for Koreans. REI is composed of 3 factors, such basal metabolic rate(or Resting Energy Expenditure, REE), activity energy, and thermogenic effect of foods. The first 1962 calculation formula of REI was weight based formula, that of 95's was the weight based REE multiplied by activity coefficient, and the recent one of 2005's(Estimated Energy Requirement: EER) was age, height. weight, and the activity level applying formula derived from the energy expenditure data by doubly labeled water technique(DLW). During the 50 years or so, REIs were reduced in all age groups, according to the activity(labor) strength and hour were reduced. The individual REI calculation method was introduced in 1995, and individual REI calculation was recommended since to prevent obesity. For the better REI estimation for Koreans, REI calculation formula derived from our peoples' DLW energy expenditure data is required.

• Journal of Wetlands Research
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• v.19 no.4
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• pp.491-500
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• 2017

Stream water quantity is the most basic, fundamental and important element for stream water quality and for conservation of ecological environment. This study aims to analyze causes of changes in stream water quantity based on the percentage of impervious surface area (%ISA) in urban streams of Gyeonggi-do, and also to present a method to secure water quantity of urban streams in Gyeonggi-do and data to be applied to River Management Policy. For this purpose, the Anyangcheon watershed, the Tancheon watershed, and the Osancheon watershed were selected as samples of the urban streams. In addition, the stream water quantity and the changes in stream water quality which were based on the amount of ISA, and methods to directly and indirectly secure stream water quantity were investigated. The results are as follows. The amounts of ISA of the Anyangcheon watershed, of the Tancheon watershed, and of the Osancheon watershed showed a 5.32%, 6.32%, and 7.22% increase, respectively, from 2014 which was approximately 10 years ago. The runoff coefficient generally increased as the amount of ISA was increased. Water reuse quantity of stream in the Tanchon watershed had a positive effect on securing stream water quantity, but both in the Anyangcheon watershed and in the Osancheon watershed, it did not have a positive effect on that. However, water reuse quantity of stream improved the water quality of each stream.