• International Journal of Industrial Entomology and Biomaterials
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• v.20 no.2
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• pp.61-67
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• 2010

The bivoltine tropical tasar ecoraces of Antheraea mylitta Drury produces cocoons for seed production under seed crop rearing (July-August with $22{\sim}30^{\circ}C$ temperature and 50~70% relative humidity) and for silk production under commercial crop rearing (September-December with $17{\sim}27^{\circ}C$ temperature and 60~80% relative humidity). To consider the impact of hybrid vigour on egg, larva, cocoon and silk related commercial traits, the $F_1$ hybrids made among Daba, Jata and Raily ecoraces were assessed successively for three years. The hybrid vigour in the $F_1$ hybrid of Daba$\times$Jata ($T_7$) was positive for egg fertility (+23.1%), shell weight (+25.6%), silk yield (+79.0%) and filament length (+68.1%), with filament of high denier (11.98d) and reduced larval span (-7.1%). The Jata$\times$Daba ($T_8$) hybrid has shown negative heterosis in egg fertility (-8.0%) compared to the other F1 hybrids, Daba$\times$Raily ($T_9$) and Raily$\times$Daba ($T_{10}$), when they rise simultaneously during the commercial crop season. The better performance of parental ecoraces ($T_4$ to $T_6$) in their commercial traits during commercial crop over parents of seed crop ($T_1$ to $T_3$) and the superior performance of $F_1$ hybrids ($T_7$ to $T_{10}$) over parents of commercial crop ($T_4$ to $T_6$) during commercial crop season indicates the apparent hybrid vigour in tasar $F_1$ hybrids. As the Daba$\times$Jata ($T_7$) and Jata$\times$Daba ($T_8$) $F_1$ hybrids have shown highest hybrid vigour, their rearing during commercial crop can optimize the silk productivity and commercial sustenance of the tasar silk industry.

• Current Research on Agriculture and Life Sciences
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• v.1
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• pp.67-83
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• 1983

The new microsporidia S80 isolated from, Bombyx mori L. in Korea showed ovoid in the morphology of the spores and the size were measured $2.9{\pm}0.28{\mu}$ in length and $1.7{\pm}0.29{\mu}$ width. No other microsporidian spore like this has not been so far isolated from Silkworm. The length of the polar filament extruded in hydrogen peroxide ($H_2O_2$) at $30^{\circ}C$ was $26{\mu}$ of a round cytoplasm on the top. The spores were partly stained with Giemsa, Safranin-O and Gram as the same staining properties as Nosema bombycis, Microsporidia K 79 and other microsporidian spores. The fine structures were observed under scanning eleceron microscope through ultrathin sectioning. The spore wall was composed of three layers ; the thin exospore of an electron dense rippled layer, the thick electron lucent endospore which was thinning considerably at the polar filament insertion point, and the inner limiting membrane. Polar cap present at the sporeapex, with a long polar filament of 12-13 coils, subtending angle of $60^{\circ}$ to spore axis, which is tubular made up of a multilayered and are a benes core, light ring structure enclosing the dance core, the dark ring structure enclosing the inner light ring structure and the other than and light ring structure bounded from cytoplasm. Lamellate polaroplast occupied the anterior part of the spore, and the two neclei with dense nucleoplasm bounded by a double nuclear envelope were cited in the slight downer middle portion of spore. From the characteristics of the shape, size and fine structures, it is certain to reason the Microsporidia S80 belong to the phylum Microspora, class Microspora, order Microsporida, order Microsporida. The shape of two nuclei cited seems to be genus Nosema, but in the classification for the suborder it should be defined wheather pansporoblasts be formed or not and for the genis especial attempts have been made to define the characters which distinguish the disporous genera in the life cycle. Survey through the infection of the bad cocoons during 1980 to 1982 in South Korea the areas contaminated with new microsporidia were revealed 5 provinces of Kyung-Gi, Kang-Won, Chung-Nam and Chun-Nam. Pathological effects inoculated per os at second instar larvae of silkworm, the LD 50 was $7.1{\times}10^7/ml$ as lower pathogenecity than that of Nosema bombycis Naegeli of $1.2{\times}10_7/ml$. While on the other hand the inoculation of the microsporidia at fourth instar larvae lowerd the whole cocoon weight and cocoon shell weight and significant at 1% level. The microsporidia S80 defined it can not be transmitted transovarially from the result of predictive and collective examination of 21 egg batches from the infected female moth.