• Journal of Sericultural and Entomological Science
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• v.27 no.1
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• pp.31-36
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• 1985

Using sexual differences between female and male as the indication of the degree of the physiological distrubance in the sex-limited larval marking strains or sex-limited egg colour strains, it was investigated whether the physiological distrubance caused y the translocated autosome fragment on the W-sex determination chromosome, have an effect on expression of the three quantitative characters; cocoon weight, cocoon shell weight, and cocoon shell percentage in the female silkworm. The mean values of the ratios of female to male the above two experimental groups were 127%, 107%, 85%, in cocoon shell weight, and cocoon shell percentage, respectively. On the other hand, those from the three normal stains, namely the basic stock strains, white egg strains, and hybrids were 129% for cocoon weight, 110% for cocoon shell weight, and 85% for cocoon shell percentage, respectively. From the results, it comes to the conclusion that the translocated autosome fragment on the W-chromosome has no influences on the expression of the quantitative characters of the female silkworm because sexual differences of the sex-limited strains were very similar to those of the three normal strains.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.107-112
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• 2004

The combining abilities in the 5 newly evolved thermo tolerant breeds viz., SR6, SR7, SR8 SR9 and SR10 of silkworm Bombyx mori L. and their 15 hybrids were made in a line${\times}$tester crossing programme. Data were analysed for seven quantitative traits i.e., pupation rate, cocoon yield, cocoon weight, cocoon shell weight, cocoon shell ratio, filament length and raw silk percentage under optimum room temperature $(25{\pm}{1^{\circ}C})$ conditions (In case of high temperature $(36{\pm}{1^{\circ}C})$ stress conditions five economic traits except filament length and raw silk percentage) with 3 widely adapted testers i.e., KA, CSR2 and CC1 as lines (females) and testers (males) respectively. The performance at high temperature and low humidity conditions aye only taken into consideration for selecting the best lines/hybrids. Among the lines SR6 exhibited positive General combining ability (GCA) effects for pupation rate, cocoon yield, cocoon weight and cocoon shell ratio traits, followed by SR7 for pupation rate, cocoon yield and cocoon shell weight and cocoon shell ratio. Among testers, KA exhibited positive GCA effects for two quantitative traits cocoon yield, cocoon weight and CSR2 for cocoon shell weight and cocoon shell ratio under adverse temperature conditions. The hybrid SR6${\times}$CC1 and SR7${\times}$CSR2 exhibited significant positive Specific combining ability (SCA) effects for majority of the traits in high temperature stress conditions of rearing. The better parent value of heterosis(Heterobeltiosis) was exhibited by the hybrid SR6${\times}$CC1 for pupation rate, cocoon yield, cocoon weight and cocoon shell weight and SR7${\times}$CSR2 for all the trails evaluated under high temperature conditions. Based on the results, the lines SR6 and SR7 was judged as best combiners and the hybrids SR6${\times}$CC1 and SR7${\times}$CSR2 can be selected for commercial exploitation in tropical climate.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.2
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• pp.205-208
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• 2012

Antheraea pernyi silkworm is a well known wild silkworm to produce a valuable silk fiber. A. pernyi silkworm was reared in Korean oak field and examined the cocoon characteristics, such as cocoon weight, cocoon shell weight, and percentage of cocoon shell weight. Degumming loss was also measured after alkali degumming process. A. pernyi silkworm spins tawny color cocoon in oval shape. Cocoon shell weight of A. pernyi silkworm, 0.78 g, was heavier than that of B. mori silkworm, 0.51 to 0.63 g. Cocoon shell percentage of A. pernyi silkworm, 32.8%, was higher than that of B. mori silkworm, 23.4 to 25.2%. Degumming loss percentage of A. pernyi silkworm, 17.1%, was lower than that of B. mori silkworm, 25.0%. SEM showed that the surface of the cocoon filament was coarse and oriented with longitudinal direction.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.1
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• pp.15-20
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• 2003

The present investigation was carried out to study the possible cause for reciprocal difference in silkworm hybrids. By utilising the polyvoltine race Pure Mysore (PM) and newly evolved breeds (CSR2, CSR5, CSR16 and CSR17), the direct and reciprocal crosses of polyvoltine ${\times}$ bivoltine and also bivoltine hybrids were studied. The hybrids of polyvoltine ${\times}$ bivoltine (direct) are superior to their reciprocal crosses in respect of cocoon yield, cocoon weight and filament length. The reciprocal crosses of polyvoltine ${\times}$ bivoltine are superior to their direct crosses in respect of fecundity and short larval duration. No significant differences were observed in the characters like cocoon shell ratio, raw silk percentage, denier, reelability and neatness in both polyvoltine ${\times}$ bivoltine direct crosses and their reciprocals. The expression of cocoon characters as a function of sex revealed that direct crosses (polyvoltine ${\times}$ bivoltine) showed higher cocoon weight, pupal weight, shell weight and longer filament length in females than the reciprocal crosses (bivoltine ${\times}$ polyvoltine), where as these characters in males were almost the same in both direct and reciprocal crosses, indicating that the sex-linked genetic factor played a more important role. it was clear that difference in cocoon yield observed in reciprocal crosses of polyvoltine ${\times}$ bivoltine was due to the low cocoon and shell weight in females which was turn due to presence of early maturity genes (Lme) linked with sex-chromosome (X) which effect on larvae period of the silkworm. In bivoltine hybrids, i.e., both direct and their reciprocals crosses, all the characters viz., hatching percentage, larval duration, survival, cocoon weight, cocoon shell weight, cocoon shell ratio, raw silk percentage, filament length, denier, reelability and neatness did not show any significant difference (except number of eggs laid by moth) which could account for presence of same maturity genes (Lm) in both direct and reciprocal crosses. it was clear that reciprocal differences occur when the hybrids are prepared from the parental strains with different voltinism.

• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.1
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• pp.135-139
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• 2002

By means of correlation analysis and path coefficient analysis, 18 characteristics of 325 conserved silkworm races were studied for the selecting efficiency on main silk quality characteristics. The result showed that selecting lines with heavier cocoon weight are beneficial to raise the weight of have. Choosing strains with higher cocoon shell percentage, longer filament length and better reelability percentage is good for increasing non-broken filament length. Selecting lines with medium daily weight of have produced in the fifth instar, medium cocoon shell percentage and simultaneously selecting heavier cocoon shell weight are favorable to promote reelability percentage. Choosing strains with heavier cocoon weight, higher cocoon shell percentage, heavier daily weight of bave produced in the fifth instar and heavier weight of bave but shorter filament length may hopefully breed out a race with thick filament size. And selecting strains with heavier weight of have and fine filament size are beneficial to increase filament length. Selecting lines with higher cocoon shell percentage and heavier weight of bave are favorable to raise raw silk percentage of cocoon. There are no significant direct or indirect correlation coefficient between viability and major characteristics of silk quality. It is, therefore, feasible to breed a silkworm race with excellent vitality and good silk quality as well.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.2
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• pp.149-153
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• 2002

Induction of non-diapause eggs and manifestation of quantitative characters were studied in different seasons by low temperature incubation of eggs of a low yielding diapause with coloured cocoons silkworm breed, RDI of Bombyx mori. Hundred percent of nandiapause eggs were induced when the egg incubation was carried out at 15$^{\circ}C$ followed by incubation initially at $25^{\circ}C$ and then at $15^{\circ}C$. The diapause-nondia-pause relation was found to be reversible. Analysis of variance study demonstrated significant variation in all the quantitative characters whereas significant variation due to different incubation treatments was observed for larval span, fifth instar larval span, cocoon yield/10,000 larvae by weight, cocoon weight, cocoon shell weight, and cocoon shell ratio. study on hybrid vigour skewed significant heterosis over mid parent value for four economic characters viz., cocoon weight, cocoon shell weight, cocoon shell ratio and filament length in a Fl hybrid between RD1 with diapause eggs and Japanese type bivoltine $NB_{4} D_{2}$.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.1
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• pp.23-30
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• 2002

The general and specific combining abilities and hybrid vigour of seven polyvoltine breeds [BL24, BL62, BL6S, BL67, BL68, BL69, and Pure Mysore (PM)] with five bivoltine breeds (NB4D2, CSR2, CSR5, CSR18 and CSR19) were evaluated in a line ${times}$ tester design by analysing six quantitative traits viz., fecundity, yield 110,000 larvae both by number and weight, cocoon weight, shell weight and cocoon shell ratio. ANOVA estimates showed significant effects for all the traits indicating the role of both additive and non- additive gene actions. Estimation of GCA revealed that the breed BL67 exhibited significant positive effects toy all the six traits followed by BL68 for four important economic traits viz., fecundity, cocoon weight, shell weight and cocoon shell ratio. Among testers, CSR5 showed significant GCA effects for five traits whereas CSR2 exhibited for shell weight and shell ratio. Three hybrids viz., BL67${times}$ NB4D2, BL67 ${times}$CSR5 and BL67${times}$ CSR19 excelled in four quantitative traits for SCA. Majority of the hybrids expressed positive hybrid vigour for cocoon yield/10,000 larvae by weight, cocoon weight and shell weight. Four hybrids namely PM ${times}$ NB4D2, BL67 ${times}$NB4D2, BL67${times}$ CSR5 and BL67${times}$ CSR19 manifesting positive hybrid vigour for five economic traits were adjudicated as best Heterotic hybrids and recommended for commercial exploitation to increase the silk yield.

• Journal of Sericultural and Entomological Science
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• v.39 no.2
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• pp.205-209
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• 1997

In a line X tester crossing programme (24 lines 2 testers) the general combining ability (GCA) and specific combining ability (SCA) effects were analyzed for five economic characters in the bivoltine silkworm, Bombyx mori L. The results showed desired GCA effects in 934D1 (9500), 934B (9789) and 934A1 (9855) for cocoon yield per 10,000 larvae brushed by number. Likewise, the lines found to be superior based on GCA effects for other characters were as follows; 931D (14.040 Kgs), 935E (17.023 Kga.), 934D1 (15.643 Kgs.) and 934B (15.687 Kgs.) for cocoon yield by weight: 931D (1.717 g) and 930E (1.796 g) for single cocoon weight; 932B (0.330 g) for single shell weight: 931D (1.717 g) and 930e (1.796 g) for single cocoon weight; 932B (0.330 g) for single shell weight; 932B (18.7%), 933A (18.86%) and 935A (19.89%) for shell ratio. SCA effects showed the superiority of 932D$\times$KA (9822 cocoon yield per 10,000 larvae brushed by number); 932A$\times$NB4D2 (16.933 Kgs. cocoon yield per 10,000 larvae brushed by weight); 931C$\times$KA (1.911 g single cocoon weight); 934$\times$NB4D2 (0.371 g single shell weight and 21.0% shell ratio). The analysis indicated non-additive gene action for all the five characters.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.1
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• pp.59-63
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• 2003

In the present study, hybrid vigour in terms of heterosis and over dominance of newly evolved bivoltine hybrids and their reciprocals raised from the promising newly evolved bivoltine breeds were calculated for the important parameters like pupation, cocoon yield, cocoon weight, shell weight, shell ratio, raw silk percentage, filament length and neatness. Through there was no significant difference among the hybrids for the characters pupation, cocoon yield and cocoon weight, the magnitude of heterosis and over dominance were varied significantly for these characters. However, significant differences were observed between new hybrids and control hybrid for the characters like cocoon shell weight, cocoon shell ratio, filament length and raw silk percentage. The results indicate that majority of new hybrids have shown higher heterosis and over dominance than control hybrid. The data also indicate that highest mean heterosis of all new hybrids was recorded for cocoon yield followed by filament length. Whereas highest mean over dominance was recorded for cocoon yield followed by cocoon weight. Negative mean heterosis and over dominance were recorded for the trait cocoon shell ratio. Based on the results, the importance of three hybrids viz., CSR2 ${\times}$ CSR4, CSR2 ${\times}$ CSR5 and CSR3 ${\times}$ CSR6 and their reciprocals (CSR4 ${\times}$ CSR2, CSR5 ${\times}$ CSR2 and CSR6 ${\times}$ CSR3) which exhibited significant heterosis and over dominance, for commercial exploitation was discussed.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.1
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• pp.75-78
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• 2005

Inheritance of cocoon and shell weight characters in silkworm crosses between sex-limited at larva stage lines, including three (NIG$_{1}$ $\times$ TV$_{3/2}$ ,NIG$_{1}$ $\times$ TBV$_{2/24}$ and NIG$_{1}$ $\times$ B$_{2/6}$ ) from Japanese type and three (NIG$_{2}$ $\times$ B$_{2/6}$ ,TV$_{3/2}$ $\times$ NIG$_{1}$ and NIG$_{2}$ $\times$ TBV$_{2/24}$ ) from Chinese type was studied at Regional Centre for Scientific­Applied Service - Vratza during the period of 2000 ­2002. Inheritance of tested characters in F I was deter­mined through genetic parameters, additiveness (a), dominance (d) and their ratio (d/a). Degree and frequency of transgressions in F$_{2}$ were calculated also. Over dominance (d/a(>) 1) with bigger contribution of the parent with higher value was determined at inheritance tested characters. Transgressions obtained at cocoon and shell weight characters were positive and varied in wide limits. The productivity of parent's lines had defined the variability of genetic parameters for degree and frequency of transgressions. Combination of positive transgressions at cocoon and shell weight characters in F2 hybrid populations from sex-limited at larva stage allow us to select individuals with high productive potential for further use in selection programs.