• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 공동 춘계학술대회
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• pp.56.2-56
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• 1996

No Abstract, See Full Text

• 한국응용곤충학회지
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• 제33권2호
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• pp.74-80
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• 1994

바퀴에 대한 dichlorvos(DDVP) 약제로 11세대 누대 도태한 계통에 대한 저항성 유발정도, 교차저항성 및 효소활성을 조사한 결과는 반수치사농도 비교시 감수성에 비하여 8.58배 증가하였고 교차정항성은 chlorpyrifos, propowur, fenvalerate 와 permethrin 약제에서 각각 3.35, 4.09, 2.83 및 2.00배의 저항성을 보였으며 효소활성은 filter paper testdptjrka수성 대비 33%가 증가하였고 전기영동시험에서는 4개의 band인 esterase-1,2,4 및 7 band 가 확인되었다.

• 한국응용곤충학회지
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• 제32권1호
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• pp.24-29
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• 1993

바퀴 (Blattella germanica L.) 의 효과적인 방제대책 수립을 위한 기초 자료로 활용하고자, 바퀴 방제에 사용되어지고 있는 6종의 살충제를 이용하여 생물검정 방법에 따른 살충력의 차이를 실험한 결과, Dry film법에 따른 약제의 살충력은 수컷 바퀴의 반수치사농도($LC_{50}$, ppm)가 chlorpyrifos 1.79, fenvalerate 1.87, propoxur 3.04, permethrin 4.37, DDVP 32.72, hydramethylnon 270.81로서 암컷 역시 같은 순위의 살충력을 보였다. 미량국소처리법에 따른 약제의 살충력은 수컷 바퀴의 반수치사약량( $LC_{50}$, $\mu$g/g)이 DDVP와 fenvalerate가 2.63, chlorpyrifos 3.79, permethrin 4.51, propoxur 6.73, hydramethylnon 44.99로써 암컷 역시 같은 순위의 살충력을 보였다. 식이법에 따른 약제의 살충력은 수컷 바퀴의 CT(Concentration % $\times$ $LC_{50}$)값의 비교에서 유기인계 살충제인 chlorpyrifos가 속효성이였고, 피레스로이드계 살충제인 hydramethyInon은 지효성이 좋은 살충제로 선발되었다.

• Parasites, Hosts and Diseases
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• 제58권5호
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• pp.537-542
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• 2020

Cockroaches inhabit various habitats, which will influence their microbiome. Although the microbiome can be influenced by the diet and environmental factors, it can also differ between species. Therefore, we conducted 16S rDNA-targeted high-throughput sequencing to evaluate the overall bacterial composition of the microbiomes of 3 cockroach species, Periplaneta americana, P. japonica, and P. fuliginosa, raised in laboratory for several generations under the same conditions. The experiments were conducted using male adult cockroaches. The number of operational taxonomic units (OTUs) was not significantly different among the 3 species. With regard to the Shannon and Pielou indexes, higher microbiome values were noted in P. americana than in P. japonica and P. fuliginosa. Microbiome composition was also evaluated, with endosymbionts accounting for over half of all OTUs in P. japonica and P. fuliginosa. Beta diversity analysis further showed that P. japonica and P. fuliginosa had similar microbiome composition, which differed from that of P. americana. However, we also identified that P. japonica and P. fuliginosa host distinct OTUs. Thus, although microbiome compositions may vary based on multiple conditions, it is possible to identify distinct microbiome compositions among different Periplaneta cockroach species, even when the individuals are reared under the same conditions.

• 한국응용곤충학회지
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• 제59권1호
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• pp.5-13
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• 2020

바퀴는 혐오감을 줄 뿐만 아니라 여러 종류의 병원체를 기계적으로 전파하는 주요한 위생곤충이며, 바퀴 방제를 위해 독먹이제를 가장 많이 사용한다. 본 연구에서는 4종류(chlorpyrifos, avermectin, hydramethylnon 및 fipronil)의 살충 주성분이 각각 함유된 국내 유통 독먹이제 3종(Korea Combat Ultra Slim (K-CUS), Korea Combat Power (K-CP) 및 Korea Zaps Dual Bait (K-ZDB))과 미국 유통 독먹이제 3종(US Combat Source Kill Max (US-CSKM), US Hot Shot MaxAttrax Roach Bait (US-HSM) 및 US Raid Double Control Small Roach Bait (US-RDC))의 독일바퀴(Blattella germanica)에 대한 살충효력을 검증하였다. 6종의 독먹이제 중 fipronil을 살충 주성분으로 사용하는 세 가지의 독먹이제(K-CUS, K-CP 및 US-CSKM)가 속효성 및 높은 살충효과를 나타내었다. K-CUS과 K-CP의 살충효력의 지속성을 검정하기 위해 두 제품을 개봉 후 6개월 및 12개월 이후에 추가로 생물검정을 실시한 결과, 두 제품 모두 시간이 지남에 따라 제품 개봉 직후에 비해 살충효과가 감소하는 경향을 보였지만, 40시간 이내 독일바퀴에 대한 사충률이 90%에 도달하는 것을 감안하면 적어도 이들 독먹이제의 옥내 설치 후 1년동안은 살충효과가 유지되는 것으로 판단된다.

• 농약과학회지
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• 제12권2호
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• pp.162-167
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• 2008

제한된 주거공간에서 바퀴의 방제효과를 높이기 위하여 한쪽에 기피성분(push)을 처리하고 반대쪽에 일반적인 유인성분(pull)이 포함된 독먹이제(bait)를 처리함으로서 행동자극에 의한 기피-유인 방제효과를 검증하였다. 먼저 상형장치를 이용한 mini-field 시험에서, 바퀴는 기피제형이 처리된 장소로부터 벗어나서 유인 먹이가 처리된 곳으로 몰려들었으며, 처리된 유인먹이와 독먹이의 소비량은 기피-유인 효과에 의해 밀집된 곳의 먹이에서 높게 나타났다. 따라서 바퀴에 대하여 기피 효과가 우수한 식물정유를 선별 적용함으로서 살충효과를 높이기 위하여 Citrus 속의 grapefruit, lemon, lime, orange, 그리고 petitgrain 오일에 대한 기피-유인 살충효과를 평가하였다. 동시 처리된 기피-유인 처리는 유인 독먹이만 처리된 대조구에 비해 더 빠르고 높은 살충효과를 나타냈으며, Citrus 속 오일의 기피에 의한 살충효과는 grapefruit > lemon > lime > orange > petitgrain 오일 순으로 나타났다. 따라서 본 실험은 제한된 공간에서 기피제로서 Citrus속 오일과 기피-유인 방법을 이용하는 효율적인 방제법을 제시한다.

• Parasites, Hosts and Diseases
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• 제61권1호
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• pp.60-71
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• 2023

Cockroaches can cause allergic sensitization in humans via contact with their feces or frass. Antibiotics can affect concentration of major allergen and total bacteria production in German cockroaches (Blattella germanica). This study examined the ability of antibiotic-treated German cockroaches to induce allergic airway inflammation and the effect of antibiotics on their lipopolysaccharide and Bla g1, 2, and 5 expression levels. Specifically, we measured the ability of German cockroach extract (with or without prior antibiotic exposure) to induce allergic inflammation in human bronchial epithelial cells and a mouse model of asthma. Bacterial 16S rRNA and lipopolysaccharide levels were lower in ampicillin-treated cockroaches than in the control group. The Bla g1, Bla g2, and Bla g5 expression in ampicillin-treated cockroaches decreased at both the protein and RNA levels. In human bronchial epithelial cell lines BEAS-2B exposed to the ampicillin-treated extract, expression levels of interleukin-6 and interleukin-8 were lower than that in the control group. The total cell count and eosinophil count in bronchoalveolar lavage fluid was also lower in mice exposed to the ampicillin-treated extract than in those exposed to normal cockroach extract. Mouse lung histopathology showed reduced immune cell infiltration and mucus production in the ampicillin group. Our results showed that ampicillin treatment reduced the symbiont bacterial population and major allergen levels in German cockroaches, leading to reduced airway inflammation in mice. These results can facilitate the preparation of protein extracts for immunotherapy or diagnostics applications.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2000년도 합동 춘계 학술발표회
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• pp.39-39
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• 2000

• Parasites, Hosts and Diseases
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• 제43권3호
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• pp.101-110
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• 2005

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863 bp open reading frame which encodes for 257 amino acids. The deduced amino acid sequence exhibited $42-57\%$ homology with the serine protease from dust mites, and consisted of a conserved catalytic domain (GOSGGPLV). bgtryp-1 was determined by both Northern and Southern analysis to be a 0.9 kb, single-copy gene. SDS-PAGE and Western blotting analyses of the recombinant protein (Bgtryp-1) over-expressed in Escherichia coli revealed that the molecular mass of the expressed protein was 35 kDa, and the expressed protein was capable of reacting with the sera of cock-roach allergy patients. We also discussed the possibility that trypsin excreted by the digestive system of the German cockroach not only functions as an allergen, but also may perform a vital role in the activation of PAR-2.

• Animal cells and systems
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• 제6권1호
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• pp.65-68
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• 2002

Female genital morphology of Cryptocercus Kyebangensis is described, focusing on the structural features of ovipositors at each developmental stage. Ovipositor valves were the first genital structures to appear in female nymphs. The caudal margin of the 9th sternum was nearly straight in the 1st instars of both sexes, with a slight median notch, but was deeply concave in the female nymphs of the End instar. The 1st valve of ovipositor budded off from membranous fold between the 8th 8nd 9th sternum and a pair of the 2nd valve came from the 9th sternum. Separation of styli occurred in the female nymphs of the 8th or 9th instar. Some parts of the female genitalia were sclerotized after the final molt. Spermathecae were the fork type and leaned a little to the right in the middle line of the genital chamber from ventro-posterior view. The trace sealed with sclerite materials existed on the spermathecal opening. The genital segments (segments Ⅷ, IX, and X) were concealed completely by the 7th tergum and sternum posteriorly expanded. Terminal lobes which exist in the Blattidae were not observed in C. Kyebangensis. Instead, the apicolateral emargination existed on the terminal abdominal segment of C. Kyebangensis females.