• 한국식품저장유통학회지
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• 제11권4호
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• pp.491-495
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• 2004

천연 저 칼로리 감미료로써 stevia의 소비가 증가하고 있는 시점에서 재배 및 기능성 식품소재로 이용하기 위하여 수확시기별 특성을 조사한 결과는 다음과 같다. 가용성 고형분 함량과 추출수율은 성장 기간이 길어질수록 증가하였고, pH는 감소하는 경향을 보였다. 색도와 투과율은 큰 변화가 없었으나, 성장 시기가 길어질수록 L, a, b값은 유사한 감소 경향을 나타내었고, 갈색도는 증가하였다. 성장기간 중 stevioside와 rebaudioside A의 비율은 stevioside는 $68.97\%$에서 $61.09\%$까지 감소하고, rebaudioside A는 $20.83\%$에서 $27.52\%$까지 증가하였다. Stevia 잎의 감미성분함량은 성장함에 따라 일정한 수준으로 증가하여 최고치는 8월말이며 함량은 $12.80\%$였고, 9월말에는 함량이 $12.10\%$로 감소하는 경향을 보였다. 최고 감미도는 25 - 30배였으며 감미질은 5월말에 채취한 시료가 가장 우수하였다.

• 대한한방부인과학회지
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• 제19권2호
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• pp.92-106
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• 2006

Purpose : To address the ability of Olibanum to induce cell death, we investigated the effect of olibanum on cell apoptosis. Twenty-four hours later, apoptosis occurred following olibanum exposure in a dose-dependent manner. Methods : We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below $37^{\circ}C$, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : The treatment of BAPTA-AM regulated olibanum-induced apoptosis in HeLa human cervical carcinoma cells. The 24 hr-earlier -thapsigargin-pretreated cell showed the resistance against olibanum-induced apoptosis and the Ru360-mitochondrial uniporter-inhibited olibanum-induced apoptosis, too. It means that olibanum leads to the accumulation of calcium and the resultant apoptosis in HeLa cells. Immunoblotting data also shows that the expression of GRP78, ER stress marker protein, was induced by the olibanum. Bcl-2, anti-apototic protein, was decreased and that the expression of Bax, pro-apoptotic protein, was increased by the addition of olibanum. Interestingly, the olibanum increased the activity of caspase-8 as well as calpain cysteine pretense in HeLa cervical carcinoma cells. Calpain inhibitor-calpastatin as well as caspase-8C/A expression abrogated olibanum-induced apoptosis in the carcinoma cells. The inhibition of caspase-8 regulated olibanum-induced calpain activation but the inhibition of calpain did not have any effect on the caspase-8 activation in HeLa human cervical carcinoma cells. Conclusion : We conclude that olibanum induces the accumulation of calcium and the resultant apoptosis in which caspase-8 and calpain are involved.

• 대한한방부인과학회지
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• 제19권2호
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• pp.77-91
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• 2006

Purpose : To address the ability of Dohaekseungkitang (DST: a commonly used herb formulation in Korea, Japan and China to have anti-cancer effect on cervical carcinoma), we investigated the effects of DST on programmed cell death (apoptosis) in HeLa human cervical carcinoma cells. Methods : We cultured HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below $37^{\circ}C$, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : After the treatment of DST for 48 hours, apoptosis occurred in a dose-dependent manner. In this study, we have shown that DST induces calpain and the associated caspase-8 and -9 activations. Apoptosis was prevented by pre-incubation of the cells with the calcium cHeLator-BAPTA-AM, calcium channel blocker-Nif edipine or Ryonidine agonist-Ryonidine peptide, implicating calcium in the apoptotic process. Ubiquitous calpains (mu- and m-calpain) have been repeatedly implicated in apoptosis, especially in calcium-related apoptosis. However this study showed 1hat either calpain inhibitor-calpastin or caspase-3 inhibitor-DEVD- did not blocked the herb formulation-induced apoptosis in HeLa human cervical carcinoma cells. D ST initiates a cell death pathway that is partially dependent of caspases. DST-induced apoptosis requires caspase-independent mechanism. Conclusion : We conclude that DST-induced calpain activation triggers the intrinsic apoptotic pathway in which caspase-independent mechanism is also involved.

• 한국미생물·생명공학회지
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• 제19권5호
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• pp.456-463
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• 1991

Bif. longum KCTC 3215에 의한 Beta-galactosidase의 최적생성조건은 탄소원으로 lactose 1.0, 초기 pH 7.0, 배양온도 $37^{\circ}C$ 및 배양시간 17시간 후였다. 이 효소는 protamine sulfate, ammonium sulfate, DEAE-Sephadex A-50 ion exchange chromatography 및 Sephadex G-150 gel filtration 등 4단계 정제과정을 거쳐 9.25배 정제되었다.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.241-247
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• 2016

Natamycin is a widely used antifungal antibiotic. For natamycin biosynthesis, the gene pimE encodes cholesterol oxidase, which acts as a signalling protein. To confirm the positive effect of the gene pimE on natamycin biosynthesis, an additional copy of the gene pimE was inserted into the genome of Streptomyces gilvosporeus 712 under the control of the ermE* promoter (p_ermE*) using intergeneric conjugation. Overexpression of the target protein engendered 72% and 81% increases in the natamycin production and cell productivity, respectively, compared with the control strain. Further improvement in the antibiotic production was achieved in a 1 L fermenter to 7.0 g/l, which was a 153% improvement after 120 h cultivation. Exconjugants highly expressing pimE and pimM were constructed to investigate the effects of both genes on the increase of natamycin production. However, the co-effect of pimE and pimM did not enhance the antibiotic production obviously, compared with the exconjugants highly expressing pimE only. These results suggest not only a new application of cholesterol oxidase but also a useful strategy to genetically engineer natamycin production.

• Journal of Microbiology and Biotechnology
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• 제22권5호
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• pp.668-673
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• 2012

Biohydrogen production from organic wastewater by anaerobically activated sludge fermentation has already been extensively investigated, and it is known that hydrogen can be produced by glucose fermentation through three metabolic pathways, including the oxidative decarboxylation of pyruvic acid to acetyl-CoA, oxidation of NADH to $NAD^+$, and acetogenesis by hydrogen-producing acetogens. However, the exact or dominant pathways of hydrogen production in the anaerobically activated sludge fermentation process have not yet been identified. Thus, a continuous stirred-tank reactor (CSTR) was introduced and a specifically acclimated acidogenic fermentative microflora obtained under certain operation conditions. The hydrogen production activity and potential hydrogen-producing pathways in the acidogenic fermentative microflora were then investigated using batch cultures in Erlenmeyer flasks with a working volume of 500 ml. Based on an initial glucose concentration of 10 g/l, pH 6.0, and a biomass of 1.01 g/l of a mixed liquid volatile suspended solid (MLVSS), 247.7 ml of hydrogen was obtained after a 68 h cultivation period at $35{\pm}1^{\circ}C$. Further tests indicated that 69% of the hydrogen was produced from the oxidative decarboxylation of pyruvic acid, whereas the remaining 31% was from the oxidation of NADH to $NAD^+$. There were no hydrogen-producing acetogens or they were unable to work effectively in the anaerobically activated sludge with a hydraulic retention time (HRT) of less than 8 h.

• 대한한방부인과학회지
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• 제18권4호
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• pp.10-23
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• 2005

Purpose : This study is to examine the ability of Rhizoma Scirpi (RS) to induce HeLa cell viability. Methods : We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below $37^{\circ}C$, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : 1. RS induces mitochondria membrane potential collapse. 2. P38 MAPK is involved in RS-induced death in HeLa cells. 3. P38 MAPK is involved in RS-induced apoptosis in HeLa cells. 4. P38 MAPK reguates RS-induced caspase-3, -8 and -9 activation in HeLa cells. 5. The inhibition of caspase regulates RS-induced cell death in HeLa cells. 6. RS induces mitochondria membrane potential collapse in HeLa cells. 7. P38 MPK is involved in the regulation of Bcl-2 and Bfu in HeLa cells.8. RS regulates the expression of Bcl-2 and Bax in HeLa cells. 9. SR induces p38 MAPK activation in HeLa cells. Conclusion : RS induces apoptosis in HeLa cells via p38 MAPK activation.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2015년도 추계학술대회
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• pp.623-625
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• 2015

최근 발전소 온배수가 수열에너지로서 신재생에너지의무할당제(RPS)에 포함됨으로써 농업 및 수산업에 온배수를 활용하려는 움직임이 커지고 있는 상황이다. 특히 농업 및 수산업에서 에너지가 차지하는 비용이 큼으로써 에너지 효율에 대한 관심이 증대하고 있다. 네델란드 같은 선진국에서는 시설원예에서 효율적인 에너지 사용과 재배작물별 에너지 소요량등과 같은 데이터를 ICT 기반의 진단 시스템을 개발하여 농업 종사자에게 배포하고 있다. 국내에서도 향후 발전소 온배수를 이용한 시설농업의 확산에 대비하여 해외 농업 선진국의 에너지 활용 사례(Greenergy) 와 발전소 온배수 활용에 대한 에너지관리시스템(EMS : Energy Management System)을 제안하고자 한다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권2호
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• pp.144-149
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• 2001

For the production and purification of a single chain human insulin precursor, four types of fusion peptides $\beta$-galactosidase (LacZ), maltose binding protein (MBP), glutathione-S-transferase (GST), and (His)(sub)6-tagged sequence (HTS) were investigated. Recombinant E. coli harboring hybrid genes was cultivated at 37$\^{C}$ for 1h, and gene induction occurred when 0.2mM of isopropyl-D-thiogalactoside (IPTG) was added to the culture broth, except for E. coli BL21 (DE3) pLysS harboring a pET-BA cultivation with 1.0mM IPTG, followed by a longer than 4h batch fermentation respectively. DEAE-Sphacel and Sephadex G-200 gel filtration chromatography, amylose affinity chromatography, glutathione-sepharose 4B affinity chromatography, and a nickel chelating affinity chromatography system as a kind of immobilized metal ion affinity chromatography (IMAC) were all employed for the purification of a single chain human insulin precursor. The recovery yields of the HTS-fused, GST-fused, MBP-fused, and LacZ-fused single chain human insulin precursors resulted in 47%, 20%, 20%, and 18% as the total protein amounts respectively. These results show that a higher recovery yield of the finally purified recombinant peptides was achieved when affinity column chromatography was employed and when the fused peptide had a smaller molecular weight. In addition the pET expression system gave the highest productivity of a fused insulin precursor due to a two-step regulation of the gene expression, and the HTS-fused system provided the highest recovery of a fused insulin precursor based on a simple and specific separation using the IMAC technique.

• 농업과학연구
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• 제21권1호
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• pp.60-66
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• 1994

하수 및 토양시료로부터 다수의 LAS분해세균을 분리하고 고농도 (LAS 200ppm)의 액체 배지에서 생육하면서 분해력이 높은 KL-3, SH-2, EN-1등 3개 균주를 선발하였다. 이들의 형태적, 생리적 및 배양적 성질에 따라 KL-3은 Klebsiella속, SH-2는 Shigella속, EN-1은 Enterobacter속 균주로 각각 동정되었다. 실험실용 발효조에서의 LAS의 분해양상은 배양초기에 분해속도가 빠르고 (1 일후의 분해율: KL-3 및 SH-2, 50%; EN-1, 20%), 배양 1-2일사이에서 그 속도가 크게 지연되다가 다시 분해가 빠르게 진행되며 3, 4일후에는 다시 늦어지는 경향을 나타내었다 (7일후의 분해율: KL-3, 85%; SH-2, 82%; EN-1, 75%). 균체의 생육은 배양 2일 후에 $10^8cfu/mL$수준의 정지기에 도달하였으며 pH는 최초 7.0에서 6.2~6.7의 범위로 저하되어 서서히 변화하는 경향이었다. 균체에 의한 LAS의 흡착량은 Shigella sp. SH-2균주의 경우가 가장 많았으며 Freund1ich흡착등식 Y= 0.030X + 0.95를 나타내었다.