• Title/Summary/Keyword: co-cultivation

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Effects of Bio-degradable Mulches on the Yield of Maize and the Density of Soil Microbe

  • Lim, Soo-Jeong;Lee, Min-Bum;Kim, Se-Won;Kim, Jang-Su;Heo, Su-Jeong;Choi, Seung-Chul;Yoon, Byeong-Sung;Kim, In-Jong
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.4
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    • pp.375-380
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    • 2016
  • The use of polyethylene film has a problem such as increasing rural environmental contamination, collection costs and farmers' workload. The objective of this study was to evaluate bio-degradable films in terms of yield of maize and soil environment. Treatments were bio-degradable film A (BDF A), bio-degradable film B (BDF B), high density polyethylene (HDPE), and non-mulched (NM) soil. Daily mean values of soil temperature (10 cm depth) under BDF A, BDF B, and HDPE were higher than in NM soil by 2.2, 2.8, $3.1^{\circ}C$ respectively. In the mulching cultivation of maize, bio-degradable film began to degrade from 50~60days after the planting. The degradation was much progressed in the harvest time and almost decomposed in the following spring. The weight of ear of maize was not shown significantly by mulching treatments. There were little changes of soil chemical properties for the bio-degradable film mulching. After using bio-degradable films, the contents of biomass-C and dehydrogenase activity increased from 92 to $137{\sim}147mg\;kg^{-1}$, and from 87 to $123{\sim}168mg\;kg^{-1}$ respectively.

Physiological Studies on the Formation of Hairy Root by the A. rhizogenes. III. Attachment of A. rhizogenes strain A4 to Carrot(Daucus carota L.) Cells (Agrobacterium rhizogense에 Hairy Root 형성에 대한 생리학적 연구. III. 당근 세포에의 A. rhizogenes의 부착)

  • Hwang, B.;Hwang, S. J.;Ann, J. C.;Jo, H. S.
    • KSBB Journal
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    • v.4 no.2
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    • pp.94-98
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    • 1989
  • In vitro attachment experiments of bacteria to surface of host plant cell were carried out using C14 labeled cells of A. rhizogenes strain A4 and carrot protoplasts isolated from suspension culture of cells. Protoplasts were cocultivated with A. rhizogenes at various times after their isolation. Attachment kinetics showed that adherence of bacteria to protoplasts attained a maximum level within 120mins of co-cultivation. Maximum attachment occured at pH 6.0 and 24-35$^{\circ}C$. Bacterial attachment was observed at botg carrot cells with and without primary cell wall. The inhibition of transformation on the carrot root discs by A. rhizogenes was observed when non-related strain and heat inactivated bacterial strain cells were pretreated.

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Growth Characteristics of Variety of Oyster Mushroom (Pleurotus ostreatus) as Affected by Number of Air Exchanges (느타리버섯의 품종별 환기횟수에 따른 생육특성)

  • Jang, Myoung-Jun;Ha, Tae-Moon;Lee, Yun-Hae;Ju, Young-Cheol
    • Journal of Bio-Environment Control
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    • v.18 no.3
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    • pp.208-214
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    • 2009
  • In this study, we investigated the effects of the number of air exchanges (NAE) on shape fruit body in two oyster mushrooms, Chunchu 2# and Suhan 2#. The suitable NAEs of Chunchu 2# were $1/10h^{-1}$ at primordial induction, $1/6h^{-1}$ at early stage of growth, $1/4h^{-1}$ at middle stage of growth, $1/4h^{-1}$ at late stage of growth and those of Suhan 2# were $1/6h^{-1}$ at primordial induction, $1/6h^{-1}$ at early stage of growth, $1/4h^{-1}$ at middle stage of growth, and $1/2h^{-1}$ at late stage of growth. In those conditions, the fruit bodies grew well. $CO_2$ concentration hardly affected the primordial formation of both mushrooms. However there were ventilation disturbances over 1500ppm. For instance, the end of pileus rolled up etc. As a result, Suhan 2# required higher ventilation compared with Chunchu 2# and the lower NAE was favorable for growth.

The Extracting Methods of the Seeds of Yew(Taxus cuspidata Sieb) by Solvent Extraction and Its Efficacy (주목(朱木)씨앗 추출물의 제조방법 및 효능효과)

  • Zhoh, Choon-Koo;Kim, In-Young
    • Korean Journal of Pharmacognosy
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    • v.33 no.4 s.131
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    • pp.364-371
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    • 2002
  • Yew (Taxus cuspidata Sibe) selected cultivation as drug, food and decorative plant in Gyong-gi province in Korea. To extract the water soluble active ingredients, as a extracting method, there was extracted with 20g of dried seeds with each 20g of butylene glycol(BG) and propylene glycol(PG), and 40 mL of water mixing 72 hours at $40{\pm}5^{\circ}C$, and then they were filtrated by 400 mesh. Appearance of extract of seeds was pale brown, $pH=4.5{\pm}0.5$, $gravity=1.013{\pm}0.05$, a reflective $index=1.373{\pm}0.05$, and yield=75%. Also, to extract the high purity oil from seeds, it minutely pulverized the dried seeds and added the hexane, mixing 2 hours at $20{\pm}57^{\circ}C$. And then, this filtrated it with 400-mesh. It got the purified oil through evaporating them at $55^{\circ}C$ during under vacuum. As the results, appearance was slightly brown, gravity=0.922 acid value=0.12, saponification value=192, and it should be obtained the $40{\pm}5%$ of yield. As the efficacy evalution of cosmetic field, the antioxidative activities by NBT method were stronger 86.0% from extract of talus seeds than 52.0% from green tea extract and 35.0% from skullcap extract as well as the antioxidative activities by DPPH method were stronger 93.7% from extract of seed than 60.3% from extract of green tea and 27.1% from extract of skullcap. These are more effective than other plant extracts. The collagen synthesis rate on the activating fibroblast for Taxus cuspidata Sibe extract showed 35.43%. As the activity of the skin elasticity, PPE(porcine pancreatic elastase)-inhibitory activities of talus extract was 50.8%. Anti-inflammatory activity was more effective to be taken 41.1% of taxus seed oil than 24.2% of steady glycyrrhetinate (SG) as a control.

Study on bibliography of "Zhouyi cantong qi(周易參同契)" and the Book of Annotation ("주역참동계(周易參同契)"와 주석서에 대한 서지학적(書誌學的) 연구)

  • Im, Myung-Jin;Kim, Byung-Soo;Kang, Jung-Soo
    • Journal of Haehwa Medicine
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    • v.19 no.2
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    • pp.25-33
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    • 2011
  • Daoism is a very important subject that consists of oriental medicine(traditional east asia medicine). Among the many scriptures, The Zhouyi cantong qi (周易參同契, Token for Joining the Three in Accordance with the Book of Changes) is the main Chinese alchemical scripture. This book is composed with three kinds of subject, Zhouyi(周易, the Book of Changes), the Huanglao(黃老) Tradition and alchemy(鍊金, 爐火). The author's name is not signed but is concealed in the text. According to the traditional account, the legendary Han immortal from Guiji (會稽, in present-day Zhejiang, 古 浙江), Wei Boyang(魏伯陽), wrote it in the period between Emperor Shun and Emperor Huan of the Eastern Han (126-127 BC), after reading the Longhu jing (龍虎經, Scripture of the Dragon and Tiger). Later he transmitted it to Xu Congshi(徐從事), who appended a commentary, and to Chunyu Shutong(淳于叔通), who first circulated it in the world. While some features of this account provide significant details - especially about the reputed date of the text and about its formation having taken place in stages - the received Cantong qi(參同契) actually is not the product of a single generation of authors, but the result of several centuries of textual accretions as well as theory of three co-authorship by Wei Boyang(魏伯陽), Xu Congshi(徐從事), Chunyu Shutong(淳于叔通). It has over 6000 characters in four-word or five-word verses. Some parts of the book are in styles of prose and poem. Many scholars explain the title "cantongqi(參同契)", saying that "Can(參)" means three, "Tong(同)" means correspondence, and "Qi(契)" means unification. Through images of hexagrams of the Book of Changes, the book illustrates the thought of the Huanglao(黃老) Tradition and alchemy. Wei Boyang(魏伯陽) theorizes his own experience unifying the way of intercourse of Yin and Yang in the Book of Changes, the cultivation of spirit through spontaneity of the Huanglao(黃老) Tradition and the elixir refining of alchemy.

Optimal Culture Conditions for Production of Yellow Pigments from Monascus purpureus in Liquid Culture (액체 배양에 의한 Monascus purpureus가 생산하는 황색색소의 최적 생산조건)

  • Choi Chung-Sig;Jeon Chun-Pyo;Lee Jung-Bok;Lee Oh-Seuk;Rhee Chang-Ho;Kwon Gi-Seok
    • Food Science and Preservation
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    • v.13 no.2
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    • pp.192-197
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    • 2006
  • The optimum cultural conditions for production of yellow pigment by Monascus purpureus MMK2 were investigated in liquid culture. Monascus purpureus MMK2 was shown to give the maximum production of yellow pigment in the medium containing of 3.0% wheat flour, 0.15% $NaNO_3$, 0.25% $Na_2HPO_4\;12H_2O\;and\;0.15%\;MgSO_4\;7H_2O$. The optimal culture conditions for temperature and initial pH were $30^{\circ}C$ and 6.5, respectively. The yellow pigment production reached a maximum level at the 7th day of cultivation.

Evaluation for anaerobic germinability of rice germplasm for direct-seeding cultivation under submerged conditions

  • Rauf, Muhammad;Choi, Yu-Mi;Lee, Sukyeung;Lee, Myung-Chul;Oh, Sejong;Hyun, Do Yoon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.71-71
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    • 2017
  • Stable stand establishment is pre-requisite in direct rice seeding system for obtaining optimal yield of rice crop in rain-fed and waterlogged areas. Anaerobic condition on waterlogged soil causes low germination which significantly reduces crop yield. Due to low availability of tolerant genetic material for anaerobic germination, there is urgent need to evaluate rice germplasm for better germinability under anaerobic conditions. Seeds of the 185 rice accessions were evaluated for germination vigor and coleoptile length under anaerobic conditions. The variation among germplasm was tested for significance using analysis of variance and various multivariate components. Significant level of variation was observed among all accessions for germination vigor and coleoptiles length. Although highest mean values for coleoptiles length (2.1cm) and germination rate (60%) were observed in japonica accessions but maximum coleoptile length (4.68cm) and germination rate (96%) was found in indica genotype CO18. A highly significant and positive correlation was also observed between germination vigor and coleoptiles length, which signify the importance of elongated coleoptile under anaerobic conditions. The PCA analysis illustrated that 97.24% variation was accounted by PC1 while PC2 and PC3 explained 2.54% and 0.24% variation for germination vigor and coleoptile length. PCA scattered plot divided the accessions in four various groups. All AG tolerant accessions were included in group I. Likewise in the case of cluster analysis, two major clades (I and II) were formed. All accessions showing >40% germination rate were included in clade I, whereas all other accessions with <40% germination rate were grouped in clade II. Further more highly tolerant accessions (>80% germination) were grouped in sub-cluster IA.

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Comparison of Growth Characteristics of Ganoderma lucidum Based on Media and Environmental Conditions (배지 및 환경조건에 따른 영지버섯의 생육특성 비교)

  • Yu, Young-Jin;Choi, Kyu-Hwan;Seo, Sang-Young;Kang, Chan-Ho;Lee, Gi-Kwon;Kim, Hee-Jun
    • Journal of Mushroom
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    • v.15 no.3
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    • pp.104-110
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    • 2017
  • In order to determine the possibility of artificial cultivation of Ganoderma lucidum, a study was carried out to optimize the medium composition used for bed culture. Sawdust spawn of medium composition for optimal growth was found to be the combination of 75% oak-sawdust, 10% cotton-seed meal, and 15% beet pulp. The optimal conditions for the formation of fruiting bodies were shown to be a temperature of $28-30^{\circ}C$, light intensity of 1,000-1,500 lux, and $CO_2$ concentration of 1%.

Optimal Culture Condition for Production of Water-soluble Red Pigments by Monascus Purpureus (Monascus Purpureus의 수용성 적색색소 최적 배양조건)

  • Jeon, Chun-Pyo;Lee, Jung-Bok;Choi, Sung-Yeon;Shin, Ji-Won;Lee, Oh-Seuk;Choi, Chung-Sig;Rhee, Chang-Ho;Kwon, Gi-Seok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.4
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    • pp.493-498
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    • 2006
  • The optimal culture conditions of Monascus purpureus MMK2 for production of red pigment were investigated in submerged culture. Monascus purpureus MMK2 showed a maximal production of red pigment in the medium containing of 3.0% wheat flour, $0.15%\;NaNO_3,\;0.25%\;Na_2HPO_4\;12H_2O$ and $0.15%\;MgSO_4\;7H_2O$. The optimal culture conditions of temperature and initial pH were $30^{\circ}C$ and 6.5, respectively. The red pigment production reached to a maximal level at 7th day of cultivation.

The Effects of Jwa Kum-Whan and Soo Ryeon-Whan on the Hyperlipidemia in Rats (좌금환(左金丸)과 수련환(茱連丸)이 고지혈증(高脂血症)에 미치는 영향)

  • Kim, Yi-Heon;Seong, Nak-Sul;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.20 no.2
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    • pp.91-102
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    • 2005
  • Objectives : By examining the effects of Jwa Kum-Whan composed of Coptidis Rhizoma and Evodiae Fructus by the ratio of 6:1 the effects of Soo Ryeon-Whan and composed of Coptidis Rhizoma and Evodiae Fructus by the ratio of 1:1 on hyperlipidemia, the present study attempted to reveal the change of effects based on the ratio of combination. Methods : Jwa Kum-Whan and Soo Ryeon-Whan were injected to rats suffered from induced hyperlipidemia, and then its influence on lipid. During the cultivation of hepatocytes, Jwa Kum-Whan and Soo Ryeon-Whan were added to culture media, and the expression of the enzymes relevant to fat metabolism of hepatocytes was examined. Results : 1. Jwa Kum-Whan significantly decreased total cholesterol(Tc), triglyceride(TG), and LDL-cholesterol(LDLc) of rats suffering from hyperlipidemia induced by high cholesterol diet. Soo Ryeon-Whan decreased LDLc, but had no significant on Tc and TG. 2. Jwa Kum-Whan increased the expression of cholesterol esterase, LDL-receptor, diacylglycerol acyltransferase (DGAT), acylCoA-cholesterol-acyltransferase (ACAT), peroxisome proliferator activated receptor gamma $(PPAR{\gamma})$, peroxisome proliferator activated receptor alpha $(PPAR{\alpha})$ of cultivated hepatocytes. In addition, Soo Ryeon-Whan increased the expression of cholesterol esterase, LDL-Receptor, DGAT, $PPAR{\gamma},\;PPAR{\alpha}$ of cultivated hepatocytes, but had no significant effects on the expression of ACAT. Conclusion : Both Jwa Kum-Whan and Soo Ryeon-Whan were composed of Coptidis Rhizoma and Evodiae Fructus, but the fonner is more effective in hyperlipidemia.

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