• Journal of Mushroom
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• v.21 no.3
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• pp.160-166
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• 2023

The effects of punching treatment on mycelial culture and fruiting body productivity were investigated in a new Lentinula edodes cultivar, "Jadam", in sawdust medium for the stable production of oak mushroom. As the punching volume and number increased, the weight loss rate and color difference increased and the L value decreased. After spawn inoculation, the sawdust medium temperature and CO₂ concentration reached their highest values at 33 and 19 days of incubation, respectively. The O₂ concentration showed the lowest value on the 14^th day of incubation, which was the opposite pattern to the CO₂ concentration. As the punching volume and the number increased, the medium temperature and O₂ concentration increased, and the CO₂ concentration decreased. Higher punching volumes and numbers resulted in higher temperatures and lower CO₂ concentrations. The best fruiting body yield was 5 × 70 mm - 30 (punching diameter × depth - number), and the total yield after three cycles was 644.7 g.

• Journal of the Korean Wood Science and Technology
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• v.27 no.2
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• pp.78-86
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• 1999

The two parts of chestnut peels, outer and inner layers, were collected and investigated for cultivation of various mushrooms, i.e., Pleurotus ostreatus, Lentinus edodes, Hericium ramosum and the others. The inner layer of chestnut peels was contaminated with the mycelia of the blue fungi when collected, consisted of 17~20 % holocellulose, being a good material for growths of the above fungi. The outer layer was considered to be poor materials for fungal growth, because of lower amounts of minerals. Both mushrooms of Pleurotus ostreatus and Hericium ramosum grow well, and were produced fruiting bodies on the solid media containing only inner layers of chestnut peels. The Imhyup No.6 and Imhyup No.7 varieties of Lentinus edodes grow well, but produced few fruiting bodies in the solid media containing only inner layers of chestnut peels. The growth patterns of P. ostreatus and L. edodes were evaluated by production of $CO_2$. and considered to be different according to fungi. The inner layers of chestnut peels as agricultural by-products were considered to be a good material for mushroom cultivation, and outer layers could be used as being added with the other additives. The chestnut peels, being the environmental pollutants in rural areas, was considered to be a good substrate for mushroom cultivation.

• Journal of Mushroom
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• v.11 no.4
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• pp.208-213
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• 2013

The contents of raw materials which are components of mixed substrate for mushroom cultivation were analyzed to optimize the composition. The pure protein(amino acid) level of soybean meal was the highest, 44.02% followed by those of soybean curd residue(31.5%) and cotton seeds meal(30.6%). The non protein nitrogen(NPN) contents in crude protein of main nitrogen materials were 2.4% for soybean meal and 5.6% for dried soybean curd residue, while those of wheat bran and rice bran used as the carbon source were relatively higher, 17.6% compared to that of nitrogen supplying media. Crude protein content per price was 6.0 for rapeseed meal, indicating that it is high crude protein content per price. Nitrogen-free extract(NFE) considering as an ingredient for mycelial growth were high in alphacorn(72.9%) and wheat bran B(57.2%). Acid detergent fiber(ADF) was high in corncob, 51.88%, its use for cultivation of brown rot fungi including Lentinus lepideus should pay attention because the fungi lack complete lignin degradation activity.

• Journal of Bio-Environment Control
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• v.28 no.1
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• pp.28-37
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• 2019

We designed a system that can automatically collect, convey, and control cool air of $15^{\circ}C-20^{\circ}C$ containing carbon dioxide from a mushroom cultivation house to a strawberry plastic house. We recorded the temperature at various positions from July to August 2017. The average temperature of the green house during day and at night was maintained at $33^{\circ}C$ and $26^{\circ}C$, respectively. In the moveable three-tier cylindrical bed, the average temperature around root was maintained at $26^{\circ}C$ and $21^{\circ}C$ during day and at night, respectively. On the high-bench in the green house, the temperature was maintained at $32^{\circ}C$ and $30^{\circ}C$ during day and at night, respectively. The carbon dioxide concentration was maintained around 800-1,600 ppm in the mushroom cultivation system and 400-800 ppm in the strawberry plastic house. The growth characteristics of the strawberry treated with moveable three-tier cylindrical bed were significantly different from those of the untreated high-bench bed. In addition, during the summer season, moveable three-tier cylindrical bed showed more tendency to increase in normal fruit number (NFN) and to decrease in defective fruit number (DFN) compare to the high-bench bed. Therefore, the moveable three-tier cylindrical bed showed a tendency to be more than 2 times higher yields than that of the high-bench bed. It was confirmed that ever-bearing strawberry cultivars could be cultivated in green house due to the cool air supply from the mushroom cultivation system in the summer season.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.2
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• pp.51-61
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• 2021

As a result of examining the germination rate between ginseng varieties, Jagyongjong varieties had the highest germination rate, and Yeonpung. had the lowest germination rate. In the ginseng seed germination rate experiment, the highest germination rate and growth condition were shown in artificial soil conditions of the ratio of Peatmoss 6.5: Pearlite 2: Masato 1.5. Good soil conditions require adequate soil moisture forces during the incubation period. The cultivation of ginseng medicinal crops requires optimal soil breathability, soil pH, and soil stabilization, which are important for root breathing. Microbial activity in the soil has a great influence on the growth of ginseng. The optimum pH of the soil for ginseng cultivation is 5.0-5.5 As a result of the experiment, the soil remained in an appropriate range after a month. In general, when the EC concentration value of the soil for ginseng cultivation is 0.2 mS/cm or more, growth deteriorates, and when the EC concentration value is 0.5 mS/cm or more, concentration obstacles such as root decay occur. As a result of the analysis, the higher the concentration value of EC, the more likely it is to interfere with ginseng growth.

• Journal of Mushroom
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• v.17 no.4
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• pp.211-217
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• 2019

Currently, cultivation of mushrooms using the Information and Communication Technology (ICT)-based smart farming technique is increasing rapidly. The main environmental factors for growth of mushrooms are temperature, humidity, carbon dioxide (CO₂), and light. Among all the mentioned factors, currently, only temperature has been maintained under automatic control. However, humidity and ventilation are controlled using a timer, based on technical experience.Therefore, in this study, a Pleurotus eryngii first-generation smart farm model was set up that can automatically control temperature, humidity, and ventilation. After installing the environmental control system and the monitoring device, the environmental condition of the mushroom cultivation room and the growth of the fruiting bodies were studied. The data thus obtained was compared to that obtained using the conventional cultivation method.In farm A, the temperature during the primordia formation stage was about 17℃, and was maintained at approximately 16℃ during the fruiting stage. The humidity was initially maintained at 95%, and the farm was not humidified after the primordia formation stage. There was no sensor for CO₂ management, and the system was ventilated as required by observing the shape of the pileus and the stipe. It was observed that, the concentration of CO₂ was between 700 and 2,500 ppm during the growth period. The average weight of the mushrooms produced in farm A was 125 g, and the quality was between that of the premium and the first grade.In farm B. The CO₂ sensor was in use for measurement purposes only; the system was ventilated as required by observing the shape of the pileus and the stipe. During the growth period, the CO₂ concentration was observed to be between 640 and 4,500 ppm. The average weight of the mushrooms produced in farm B was 102 g.These results indicate that the quality of the king oyster mushroom is determined by the environmental conditions, especially by the concentration of CO₂. Thus, the data obtained in this study can be used as an optimal smart farm model, where, by improving the environmental control method of farm A, better quality mushrooms were obtained.

• Journal of Bio-Environment Control
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• v.32 no.2
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• pp.89-96
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• 2023

This study was conducted to apply with an air duct for the cooling and a utilizing cultivating method that uses the fruiting node and the defoliation to the high-temperature vertical and hydroponic cultivation of the oriental melon. The lower fruiting node (LF) was to remove all third vines generated from 5 nodes of a secondary vine. The higher fruiting node (HF) was fruiting on the third vine generated from a first node of the third vine. The direction of the stem string; upward (UW), downward (DW). Four treatment conditions were applied with the LF-UW, LF-DW, HF-UW (control), and HF-DW. The leaf age of melon leaves was measured for photosynthesis at 3 days intervals, and the fruit characteristic was conducted on 79 fruits in each treatment. The photosynthesis rate steadily increased after leaf development, reaching 20.8 μmol CO₂·m^-2·s^-1 on the 10 days, gradually increasing to 21.3 μmol CO₂·m^-2·s^-1 on the 19 days, and reaching 23.4 μmol CO₂·m^-2·s^-1 on the 32 days. After that, it lowered to 16.8 μmol CO₂·m^-2·s^-1 on the 38 days and dropped significantly to 7.6 μmol CO₂·m^-2·s^-1 on the 47 days. As a result of the fruit characteristics by fruiting nodes, the treatments of the fruit length was 12.6-13.4 cm, respectively, which was significant, and the fruit width was 7.9- 8.6 cm, respectively, was not significant. The soluble content ranged from 12.9 to 15.7°Brix, and the significance of all treatments, and higher than of LF-DW and HF-UW. The photosynthesis rate of melon leaves was good until 32 days after leaf development, but after that, the rate decreased. As for fruit quality, it was conformed that melons can be cultivated at the LF because the fruit enlargement and soluble content dose not decrease even when set at the LF. Results indicated that those can be used for LF and defoliation in the development of vertical and hydroponic cultivation method in high-temperature season.

• Journal of Marine Bioscience and Biotechnology
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• v.16 no.1
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• pp.26-35
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• 2024

Chlorella has various biotechnological applications, including in the biomedical and pharmaceutical industries, because of its advantages, including rich nutrients, fast growth rate, easy cultivation, and high biomass. We used the Agrobacterium-mediated transformation method to express human GM-CSF and EGF proteins, which are widely used in regenerative medicine, cosmetics, and pharmaceutical materials in Chlorella. The codon-optimized hGM-CSF and hEGF genes were cloned into plant binary vectors and transformed into Chlorella vulgaris using the Agrobacterium-mediated coculture transformation method. After transformation, genomic DNA PCR was performed for each C. vulgaris line that was stably subcultured on an antibiotic-resistant solid medium to confirm the insertion of hGM-CSF and hEGF into the chromosome. Furthermore, PT-PCR and protein expression of hGM-CSF and hEGF in each transformed C. vulgaris were significantly increased compared to the untransformed Chlorella. This study suggests that high-value proteins, including hGM-CSF and hEGF, which are foreign genes of C. vulgaris, can be stably expressed through the Agrobacterium-mediated Chlorella transformation system.

• Journal of Life Science
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• v.14 no.1
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• pp.121-125
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• 2004

The synergistic effect of lowered incubation temperature and CroEL/ES expression on the production of soluble form of B. macerans cyclodextrin glucanotransferase (CGTase) was studied in recombinant E. coli. pTCGTl and pGroll carrying the cgt and groEL/ES genes under the control of T7 promoter and pzt-I promoter, respectively, were co-introduced. Tetracycline (10 ng/ml) and IPTG (1 mM) were added at the early-exponential phase (2 hr) and mid-exponential phase (3 hr). Low temperature cultivation at $25^{\circ}C$ with groEL/ES expression improved the activity of CGTase by two fold, compared to $37^{\circ}C$ cultivation without chaperones. SDS-PACE analysis revealed that about 69% of CGTase in the total CGTase protein was found in the soluble fraction by overexpression of GroEL/ES and cultivation at$25^{\circ}C$, whereas 20% of CGTase was detected in the soluble fraction when E. coli was cultivated at $37^{\circ}C$ without chaperone. The amount of soluble CGTase from $25^{\circ}C$ culture with chaperone was 3.5-fold higher than that of $37^{\circ}C$ culture without chaperone. Therefore the expression of CroEL/ES and low temperature cultivation greatly enhanced the soluble production of CGTase in E. coli.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7990-8000
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• 2015

Plant factories are plant cultivation systems which produce farm products uniformly under the controlled environmental condition regardless of seasons and places. Thermal flow in the plant factory is an important parameter in cultivating plants. In this research, we study thermal flow characteristics for a hybrid plant factory with multi-layer cultivation shelves using computer simulation techniques. In order to obtain numerical solutions for thermal flow characteristics, a finite volume method was applied. We consider a low-Reynolds-number ${\kappa}-{\epsilon}$ turbulence model, incompressible viscous flows, and pressure boundary conditions for numerical simulation. Commercial software Solid Works Flow Simulation is then used to investigate characteristics of thermal flows in the plant factory applying several different inflow air velocities and arrangements of cultivation shelves. From numerical analysis results, we found that temperatures in cultivation shelves were uniformly distributed for Case 3 when the inflow air velocity was 1.6 m/s by using a blower in the plant factory. However in Case 1 lower temperature distributions were observed in test beds, TB2 and TB3, which indicated that additional temperature control efforts would be required. Average shelf temperature increased by $3^{\circ}C$ using artificial light source (DYLED47) with 50% blue and 50% red LED ratios. Korea Academia-Industrial cooperation Society.