• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.6135-6140
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• 2013

Background: Breast cancer is a common malignant tumor which affects health of women and multidrug resistance (MDR) is one of the main factors leading to failure of chemotherapy. This study was conducted to establish paclitaxel-resistant breast cancer cell line and nude mice models to explore underlying mechanisms of MDR. Methods: The breast cancer drug-sensitive cell line MCF-7 (MCF-7/S) was exposed in stepwise escalating paclitaxel (TAX) to induce a resistant cell line MCF-7/TAX. Cell sensitivity to drugs and growth curves were measured by MTT assay. Changes of cell morphology and ultrastructure were examined by optical and electron microscopy. The cell cycle distribution was determined by flow cytometry. Furthermore, expression of proteins related to breast cancer occurrence and MDR was tested by immunocytochemistry. In Vivo, nude mice were injected with MCF-7/S and MCF-7/TAX cells and weights and tumor sizes were observed after paclitaxel treatment. In addition, proteins involved breast cancer and MDR were detected by immunohistochemistry. Results: Compared to MCF-7/S, MCF-7/TAX cells had a higher resistance to paclitaxel, cross-resistance and prolonged doubling time. Moreover, MCF-7/TAX showed obvious alterations of ultrastructure. Estrogen receptor (ER) expression was low in drug resistant cells and tumors while expression of human epidermal growth factor receptor 2 (HER2) and Ki-67 was up-regulated. P-glycoprotein (P-gp), lung resistance-related protein (LRP) and glutathione-S-transferase-${\pi}$ (GST-${\pi}$) involved in the MDR phenotype of resistant cells and tumors were all overexpressed. Conclusion: The underlying MDR mechanism of breast cancer may involve increased expression of P-gp, LRP and GST-${\pi}$.

• Ocean and Polar Research
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• v.37 no.2
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• pp.127-140
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• 2015

A continuous series of 60 snow samples was collected at a 2.5-cm interval from a 1.5-m snow pit at a site on the Styx Glacier Plateau in Victoria Land, Antarctica, during the 2011/2012 austral summer season. Various chemical components (${\delta}D$, ${\delta}^{18}O$, $Na^+$, $K^+$, $Mg^{2+}$, $Ca^{2+}$, $Cl^-$, $SO_4{^2-}$, $NO_3{^-}$, $F^-$, $CH_3SO_3{^-}$, $CH_3CO_2{^-}$ and $HCO_2{^-}$) were determined to understand the highly resolved seasonal variations of these species in the coastal atmosphere near the Antarctic Jang Bogo station. Based on vertical profiles of ${\delta}^{18}O$, $NO_3{^-}$and MSA, which showed prominent seasonal changes in concentrations, the snow samples were dated to cover the time period from 2009 austral winter to 2012 austral summer with a mean accumulation rate of $226kgH_2Om^{-2}yr^{-1}$. Our snow profiles show pronounced seasonal variations for all the measured chemical species with a different pattern between different species. The distinctive feature of the occurrence patterns of the seasonal variations is clearly linked to changes in the relative strength of contributions from various natural sources (sea salt spray, volcanoes, crust-derived dust, and marine biogenic activities) during different short-term periods. The results allow us to understand the transport pathways and input mechanisms for each species and provide valuable information that will be useful for investigating long-term (decades to century scale periods) climate and environmental changes that can be deduced from an ice core to be retrieved from the Styx Glacier Plateau in the near future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.44-52
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• 2019

Industrial accidents differ considerably according to the industry and the scale of these industries. These industrial disasters have been analyzed in various ways. Therefore, a technique was developed to evaluate the disaster level among companies using the ANP method and the data derived from the disaster statistics over the past five years(2013~2017), as well as the safety information characteristics through questionnaires. The objective of the study was to determine the disaster level and safety level of a company. In this paper, six factors that are considered important for assessing the disaster level of the enterprise among the disaster statistics were selected. These factors included the industry type and size, age of employee, duration of treatment, duration of employment, and typical occurrence. Most companies use the accident rate for their disaster level to compare only with other companies. In this study, the weighting factors of the company were derived taking the characteristics of each company into consideration. Therefore, the disaster level of each company can be evaluated using the disaster statistics from each company and the derived weighting factors.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.5
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• pp.471-476
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• 1985

In order to set up a predictive model for effective spat collection of Pacific oyster, Crassostrea gigas, the survival rate and time required at each developmental stage of drifting larvae were surveyed during the period from June 13 to October 16 in 1984 at the main oyster farming area, Hansan Bay, the southern part of Korea. The advent of D-shape larvae ca. $72{\times}61{\mu}m$ long had two peaks in that area : July 16 and September 20. Umbo-shape larvae ca. $189{\times}217{\mu}m$ and full grown larvae co. $303{\times}308{\mu}m$ long also showed two peaks: July 21 and September 25 for the former, and July 28 and September 30 for the latter. About five days were required for D-shape larvae to develop to umbo-shape larvae. The instantaneous death rate was 0.24 and the daily survival rate 0.78 with a mean survival rate of $29.38\%$, at this intermorphological stage. The turnover time of umbo to full grown larvae varied from five to seven days with a instantaneous death rate of 0.15, daily survival rate of 0.86 and mean survival rate of $38.54\%$. Ten to twelve days were required for each group of the D-shape larvae to reach a full grown stage, and their mean survival rate was $11.32\%$ during this developmental period.

• Research in Plant Disease
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• v.23 no.1
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• pp.75-81
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• 2017

In May 2016, 24 peach samples showing abnormal and virus like symptoms were collected in one of major peach producing area, Yeongcheon-si, Gyeongsangbuk-do, Korea. We performed RT-PCR diagnosis for confirmation of viral infection. The diagnostic targets are 17 species of viruses and viroids that quarantine and high risk pathogens when it occur. As a results, seven species of viruses and viroids, including an unreported (Apricot pseudo-chlorotic leaf spot virus, APCLSV) and a quarantine (Peach latent mosaic viroid, PLMVd) species in Korea, were detected. For the sequence analysis of unreported virus, APCLSV, the sequence of coat protein gene were amplified and cloned. The sequence showed 97% nucleotide identity with other APCLSV isolates and compared with other seven species of reported Trichoviruses. This virus was classified as APCLSV based on the sequence and phylogenetic analysis. This isolate was named Yeongcheon. As patterns of APCLSV occurrence, all samples that APCLSV detected were co-infected with Apple chlorotic leaf spot virus (ACLSV). As properties of ACLSV, APCLSV has high possibility of wide spread disease in fruit tree farms in Korea. Therefore, it is necessary to do related researches, such as infection route and influence of disease in commercial orchards.

• Korean Journal of Environmental Biology
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• v.17 no.1
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• pp.1-9
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• 1999

Numerous observations revealed strong evidence of increased middle ultraviolet radiation or UV-B (280 ~ 320 nm) at the earth's surface resulting from stratospheric ozone depletion. UV is the waveband of electromagnetic radiation which is strongly absorbed by nucleic acids and proteins, thus causing damage to living systems. It has been recorded in the East Sea, Korea that solar UV-B impinging on the ocean surface penetrates seawater to significant depths. Recent researches showed that exposure to UV-B for as short as 2h at the ambient level (2.0 Wm$^{-2}$) decreased macroalgal growth and photosynthesis and destroyed photosynthetic pigments. These may suggest that UV-B could be an important environmental factor to determine algal survival and distribution. Some adaptive mechanisms to protect macroalgae from UV-damage have been found, which include photoreactivation and formation of UV-absorbing pigments. Post-illumination of visible light mitigated UV-induced damage in laminarian young sporophytes with blue the most effective waveband. The existence of UV-B absorbing pigments has been recognized in the green alga, Ulva pertusa and the red alga, Pachymeniopsis sp., which is likely to exert protective function for photosynthetic pigments inside the thalli from UV-damage. Further studies are however needed to confirm that these mechanisms are of general occurrence in seaweeds. Macroalgae together with phytoplankton are the primary producers to incorporate about 100 Gt of carbons per year, and provide half of the total biomass on the earth. UV-driven reduction in macroalgal biomass, if any, would therefore cause deleterious effects on marine ecosystem. The ultimate impacts of increasing UV-B flux due to ozone destruction are still unknown, but the impression from UV studies made so far seems to highlight the importance of setting up long-term monitoring system for us to be able to predict and detect the onset of large -scale deterioration in aquatic ecosystem.

• Korean Journal of Remote Sensing
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• v.27 no.4
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• pp.435-444
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• 2011

The land cover map derived from spectral features of high resolution optical images has low spectral resolution and heterogeneity in the same land cover class. For this reason, despite the same land cover class, the land cover can be classified into various land cover classes especially in vegetation area. In order to overcome these problems, detailed vegetation classification is applied to optical satellite image and SAR(Synthetic Aperture Radar) integrated data in vegetation area which is the result of pre-classification from optical image. The pre-classification and vegetation classification were performed with MLC(Maximum Likelihood Classification) method. The hierarchical land cover classification was proposed from fusion of detailed vegetation classes and non-vegetation classes of pre-classification. We can verify the facts that the proposed method has higher accuracy than not only general SAR data and GLCM(Gray Level Co-occurrence Matrix) texture integrated methods but also hierarchical GLCM integrated method. Especially the proposed method has high accuracy with respect to both vegetation and non-vegetation classification.

• Toxicological Research
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• v.22 no.4
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• pp.357-364
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• 2006

This study was undertaken to develop a reliable mice model demonstrating similar immunologic phenomena as human atopic dermatitis characterized with predominance of type-2 immune response. BALB/C mice and NC/Nga mice were sensitized twice with $100{\mu}l$ of 1% 2,4-dinitrochlorobenzene (DNCB) or vehicle (acetone : olive oil=4:1 mixture) in a week and challenged twice with $100{\mu}l$ of 0.2% DNCB or the vehicle at the following week. Mice were sacrificed at 19 days following the second DNCB or vehicle challenge for NC/Nga mice and at 28 days following the second DNCB or vehicle challenge for BALB/c mice. Upregulation of plasma 1gE, a hallmark of atopic dermatitis occurrence, was evident in the plasma obtained 4 day after the second DNCB challenge from BALB/c mice (approximately 4-fold) and NC/Nga mice (approximately 6-fold) treated with DNCB in comparison with that of the vehicle treated-control mice, and remain higher $3{\sim}4$ week after the second challenge. Ratio of plasma IgG1 versus IgG2a concentration was significantly higher in the mice treated with DNCB than the control mice, which also implies the skewed type-2 reactivity in vivo. Ratio of interleukin-4 versus interferon gamma produced in the splenic T cell culture supernatants was approximately 3-fold higher in the both strains of mice treated with DNCB than their control mice, respectively. The DNCB-treated mice demonstrated atopic dermatitis-like skin legions characterized with erythma, scaling, and hemorrhage, which was not observed with the control mice. Scratching on face or dorsal area was significantly more frequent (approximately 25-fold) in the DNCB-treated mice than the control at next day of the second DNCB challenge, and scratching frequency remains higher (approximately 4-fold) in the mice treated with DNCB than the control at 14 day following the second DNCB challenge. Overall, the mice model developed through sensitization and challenge with DNCB may be useful for research on atopic dermatitis and development of treatment materials for atopic dermatitis.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.10-17
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• 2015

This study was conducted to investigate the occurrence of weed flora in pastures in Jeju island of Korea and to establish the basic data for controlling them occurred in the pastures. Weed flora was investigated twice, May and July on 2003. There were a total of 207 weed species including 49 families. Among them 67 species were annual, 39 species were biennial and 101 species were perennial. The compositions of major plant families, Asteraceae, Poaceae, Polygonaceae, Rosaceae, Fabaceae were 17, 15, 8, 7, 6%, respectively. Sixty-eight species of exotics were occurred, as well. Rumex obtusifolius was the highest in importance analysis and the followings were in order of Trifolium repens, Artemisia princeps, Plantago asiatica, Potentilla anemonefolia and Rumex acetosella etc. Among the exotic weeds, R. obtusifolius, T. repens, R. acetosella and C. holosteoides var. hallaisanense might be most problematic weeds species in pastures in Jeju island in the future. And at the boundary of some pastures and mountains Smilax china, Duchesnea indica, Rosa maximowicziana, etc. 17 shrub species were also occurred.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.1
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• pp.1-10
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• 2018

It is difficult to predict the early-age cracks of strain restrained concrete members due to environmentally sensitive parameters. A new method is proposed to predict the cracks by test of autogenous deformation and self-induced restrained stress of specimens which simulates early-age crack state by hydration heat of the'Wall-On-Foundation'members. For this purpose, thermal analysis of entire structure considering the environmental condition is performed at first, and the specimens are set up where hydration heat was electronically controlled according to the analysis results. By measuring free deformation and force to compensate the autogenous strain including relaxation, feasibility of cracks can be estimated. The proposed method can predict the occurrence of cracks better than the material test of the early age concrete which has large variance. The method of this study is particularly useful when it is used as a preliminary experiments to predict the crack more precisely before full-scale concrete placement in construction of large structures.