• Title/Summary/Keyword: cluster analyses

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Genetic Diversity Among Waxy Corn Accessions in Korea Revealed by Microsatellite Markers

  • Park, Jun-Seong;Park, Jong-Yeol;Park, Ki-Jin;Lee, Ju-Kyong
    • Korean Journal of Breeding Science
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    • v.40 no.3
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    • pp.250-257
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    • 2008
  • Knowledge of genetic diversity and of the genetic relationships among elite breeding materials has had a significant impact on the improvement of crops. In maize, this information is particularly useful in i) planning crosses for hybrid and line development, ii) in assigning lines to heterotic groups and iii) in plant variety protection. We have used the SSR technique to study the genetic diversity and genetic relationships among 76 Korean waxy corn accessions, representing a diverse collection from throughout Korea. Assessment of genetic diversity among members of this group was conducted using 30 microsatellite markers. Among these 30 microsatellite markers, we identified a total of 127 alleles (with an average of 4.2 and a range of between 2 and 9 alleles per locus). Gene diversity at these 30 microsatellite loci varied from 0.125 to 0.795 with an average of 0.507. The cluster tree generated with the described microsatellite markers recognized two major groups with 36.5% genetic similarity. Group I includes 63 inbred lines, with similarity coefficients of between 0.365 and 0.99. Group II includes 13 inbred lines, with similarity coefficients of between 0.45 and 0.85. The present study indicates that the 30 microsatellite loci chosen for this analysis are effective molecular markers for the assessment of genetic diversity and genetic relationships between Korean waxy corn accessions. Specifically, this study's assessment of genetic diversity and relationships between a set of 76 Korean waxy corn inbred lines will be helpful for such activities as planning crosses for hybrid and line development and association mapping analyses of maize breeding programs in Korea.

Genetic diversity analysis of fourteen geese breeds based on microsatellite genotyping technique

  • Moniem, Hebatallah Abdel;Zong, Yang Yao;Abdallah, Alwasella;Chen, Guo-hong
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.11
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    • pp.1664-1672
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    • 2019
  • Objective: This study aimed to measure genetic diversity and to determine the relationships among fourteen goose breeds. Methods: Microsatellite markers were isolated from the genomic DNA of geese based on previous literature. The DNA segments, including short tandem repeats, were tested for their diversity among fourteen populations of geese. The diversity was tested on both breeds and loci level and by mean of unweighted pair group method with arithmetic mean and structure program, phylogenetic tree and population structure were tested. Results: A total of 108 distinct alleles (1%) were observed across the fourteen breeds, with 36 out of the 108 alleles (33.2%) being unique to only one breed. Genetic parameters were measured per the 14 breeds and the 9 loci. Medium to high heterozygosity was reported with high effective numbers of alleles (Ne). Polymorphic information contents (PIC) of the screened loci was found to be highly polymorphic for eleven breeds; while 3 breeds were reported moderately polymorphic. Breeding coefficient ($F_{IS}$) ranged from -0.033 to 0.358, and the pair wise genetic differentiation ($F_{ST}$) ranged from 0.01 to 0.36 across the fourteen breeds; for the 9 loci observed and expected heterozygosity, and Ne were same as the breeds parameters, PIC of the screened loci reported 6 loci highly polymorphic and 3 loci to be medium polymorphic, and $F_{IS}$ ranged from -0.113 to 0.368. In addition, genetic distance estimate revealed a close genetic distance between Canada goose and Hortobagy goose breeds by 0.04, and the highest distance was between Taihu goose and Graylag goose (anser anser) breed by 0.54. Conclusion: Cluster analyses were made, and they revealed that goose breeds had hybridized frequently, resulting in a loss of genetic distinctiveness for some breeds.

Effects of Teacher's Commnunicative Behaviors on Instructor and Class Evaluations: by Student Personalty Traits and Communication Characteristics (교수자 커뮤니케이션 행동의 차별적 효과에 대한 연구: 학습자의 성격요인과 커뮤니케이션 특성에 따른 집단간 차이)

  • Ahn, Horim
    • Journal of Digital Convergence
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    • v.19 no.1
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    • pp.361-371
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    • 2021
  • Communication education research has focused on the effects of instructor characteristics on learning. It is necessary to consider student characteristics as well to develop an effective teaching strategy. The current study tries to classify students into multiple distinct groups depending on their personality traits and communication characteristics and investigates differences in effects of teacher characteristics between those groups. A survey on unversity students was conducted to collect data. A cluster analysis was carried out to identify distint sub-groups of respondents by their personality traits and communication characteristics. The analysis yielded two distinct groups. The two groups were named stable-reticent group', and 'neurotic-talkative group' respectivley. Series of multiple regression were carried out in order to investigate differences in the effects of instructor characteristics. The analyses found different patterns of effects between the two sub-groups. The findings suggest that it is necessary to adopt different communication styles and teaching strategies depending on student types.

Geochemical Characteristics and Pollution Assessment of Surface Sediments in the Nakdong River Estuary (낙동강 하구 표층 퇴적물의 지화학적 특성 및 오염도 평가)

  • Jeon, Hye-Lyn;Lee, Hye-Yun;Yang, Deuk-Seok;Kim, Shin
    • Journal of Environmental Science International
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    • v.30 no.6
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    • pp.487-500
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    • 2021
  • To evaluate the geochemical characteristics and assess the pollution in surface sediments of the Nakdong River estuary, two sites adjacent to the estuary bank (Hh1 and Hh2) and one site at the upper part of the estuary bank (Hh3) were investigated. The surface sediments were analyzed for their contents of metals (Cu, Pb, Ni, Cr, Zn, and Al), organic matter (IL, COD, TOC, and TN), and grain size from 2018 to 2020. As a result of the pollution assessment, there was little anthropogenic contamination by most of the metals. The surface sediments in Hh2 had comparatively abundant silt and clay, whereas the other sites were mainly composed of sand. The organic index and contents of organic matter were highest at Hh2. Multivariate statistical analyses (cluster analysis and Pearson correlation analysis) showed that the contents of organic matter and pollution were associated with fine sediment. These results suggest that the geochemical characteristics were changed by the estuary bank built in the research area and that the increase in fine sediment attributable to the low-energy environment resulted in an increase in organic matter pollution.

Study on the Development of Road Safety Judgment Indicators to Establish of Installation Criteria of Safety Facility (안전시설 설치 기준 마련을 위한 도로안전 판단지표 개발연구)

  • Kim, Do Kyeong;Hwang, Jae Seong;Lee, Jae Hyeong;Lee, Cheol Ki
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.20 no.6
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    • pp.192-202
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    • 2021
  • In the past, various studies have been conducted on safety facility installation standards and road safety indices. But there are limitations in applying them to the field, such as using many survey items and variables that are difficult to use. Therefore, this study attempted to develop road safety judgment indicators considering the applicability of the research results and to prepare criteria for installing safety facilities. As part of the study, data of related systems were reviewed, and the use of variables already in use was figured out. Furthermore, the road safety judgment indicators reflecting traffic, road, and accident factors were developed through correlation and factor analysis. Later, the criteria score for determining the installation of safety facilities was derived through cluster analysis. The analyses suggested, that the installation judgment criterion score at the intersection was lower than that of the single road(crosswalk), and the road risk at the intersection was higher.

Bioinformatics Analysis of Autophagy and Mitophagy Markers Associated with Delayed Cerebral Ischemia Following Subarachnoid Hemorrhage

  • Youn, Dong Hyuk;Kim, Bong Jun;Hong, Eun Pyo;Jeon, Jin Pyeong
    • Journal of Korean Neurosurgical Society
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    • v.65 no.2
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    • pp.236-244
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    • 2022
  • Objective : To evaluate the interactions among differentially expressed autophagy and mitophagy markers in subarachnoid hemorrhage (SAH) patients with delayed cerebral ischemia (DCI). Methods : The expression data of autophagy and mitophagy-related makers in the cerebrospinal fluid (CSF) cells was analyzed by real-time reverse transcription-polymerase chain reaction and Western blotting. The markers included death-associated protein kinase (DAPK)-1, BCL2 interacting protein 3 like (BNIP3L), Bcl-1 antagonist X, phosphatase and tensin homolog-induced kinase (PINK), Unc-51 like autophagy activating kinase 1, nuclear dot protein 52, and p62. In silico functional analyses including gene ontology enrichment and the protein-protein interaction network were performed. Results : A total of 56 SAH patients were included and 22 (38.6%) of them experienced DCI. The DCI patients had significantly increased mRNA levels of DAPK1, BNIP3L, and PINK1, and increased expression of BECN1 compared to the non-DCI patients. The most enriched biological process was the positive regulation of autophagy, followed by the response to mitochondrial depolarization. The molecular functions ubiquitin-like protein ligase binding and ubiquitin-protein ligase binding were enriched. In the cluster of cellular components, Lewy bodies and the phagophore assembly site were enriched. BECN1 was the most connected gene among the differentially expressed markers related to autophagy and mitophagy in the development of DCI. Conclusion : Our study may provide novel insight into mitochondrial dysfunction in DCI pathogenesis.

Spatial distribution of halophytes and environment factors in salt marshes along the eastern Yellow Sea

  • Chung, Jaesang;Kim, Jae Hyun;Lee, Eun Ju
    • Journal of Ecology and Environment
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    • v.45 no.4
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    • pp.264-276
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    • 2021
  • Background: Salt marshes provide a variety of ecosystem services; however, they are vulnerable to human activity, water level fluctuations, and climate change. Analyses of the relationships between plant communities and environmental conditions in salt marshes are expected to provide useful information for the prediction of changes during climate change. In this study, relationships between the current vegetation structure and environmental factors were evaluated in the tidal flat at the southern tip of Ganghwa, Korea, where salt marshes are well-developed. Results: The vegetation structure in Ganghwa salt marshes was divided into three groups by cluster analysis: group A, dominated by Phragmites communis; group B, dominated by Suaeda japonica; and group C, dominated by other taxa. As determined by PERMANOVA, the groups showed significant differences with respect to altitude, soil moisture, soil organic matter, salinity, sand, clay, and silt ratios. A canonical correspondence analysis based on the percent cover of each species in the quadrats showed that the proportion of sand increased as the altitude increased and S. japonica appeared in soil with a relatively high silt proportion, while P. communis was distributed in soil with low salinity. Conclusions: The distributions of three halophyte groups differed depending on the altitude, soil moisture, salinity, and soil organic matter, sand, silt, and clay contents. Pioneer species, such as S. japonica, appeared in soil with a relatively high silt content. The P. communis community survived under a wider range of soil textures than previously reported in the literature; the species was distributed in soils with relatively low salinity, with a range expansion toward the sea in areas with freshwater influx. The observed spatial distribution patterns may provide a basis for conservation under declining salt marshes.

Variovorax terrae sp. nov. Isolated from Soil with Potential Antioxidant Activity

  • Woo, Chae Yung;Kim, Jaisoo
    • Journal of Microbiology and Biotechnology
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    • v.32 no.7
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    • pp.855-861
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    • 2022
  • A white-pigmented, non-motile, gram-negative, and rod-shaped bacterium, designated CYS-02T, was isolated from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 20-28℃ and hydrolyzed Tween 40. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CYS-02T formed a lineage within the family Comamonadaceae and clustered as members of the genus Variovorax. The closest members were Variovorax guangxiensis DSM 27352T (98.6% sequence similarity), Variovorax paradoxus NBRC 15149T (98.5%), and Variovorax gossypii JM-310T (98.3%). The principal respiratory quinone was Q-8 and the major polar lipids contain phosphatidylethanolamine (PE), phosphatidylethanolamine (PG), and diphosphatidylglycerol (DPG). The predominant cellular fatty acids were C16:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c) and summed feature 8 (C18:1ω7c and/or C18:1ω6c). The DNA GC content was 67.7 mol%. The ANI and dDDH values between strain CYS-02T and the closest members in the genus Variovorax were ≤ 79.0 and 22.4%, respectively, and the AAI and POCP values between CYS-02T and the other related species in the family Comamonadaceae were > 70% and > 50%, respectively. The genome of strain CYS-02T showed a putative terpene biosynthetic cluster responsible for antioxidant activity which was supported by DPPH radical scavenging activity test. Based on genomic, phenotypic and chemotaxonomic analyses, strain CYS-02T was classified into a novel species in the genus Variovorax, for which the name Variovorax terrae sp. nov., has been proposed. The type strain is CYS-02T (= KACC 22656T = NBRC 00115645T).

A Study on Port Improvement with the Activation of Cross-Border E-Commerce: A Study of Pyeongtaek Port

  • Choi, Hyuk-Jun;Jung, Hyun-Jae;Lee, Dong-Hyon
    • Journal of Korea Trade
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    • v.23 no.7
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    • pp.34-44
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    • 2019
  • Purpose - The purpose of this study is to present what the port of Pyeongtaek, the hub of Korean trade with China, should improve in the current situation, wherein the e-commerce trade volume between Korea and China is increasing due to the development of online technology. Design/methodology - In this study, through prior research and expert interviews on e-commerce and port activation between borders, we derived the main improvement factors for 1) Administration and Systems, 2) Facilities, 3) Transport, and 4) Manpower, and selected 12 detailed variables for the major improvement factors. To identify the relative importance of the major improvement factors, the Analytic Hierarchy Process (AHP) method was applied, and a survey was conducted among 15 related experts. Findings - As a result, among the 12 detailed variables, Composition of Association (0.267) was the first factor to be improved, followed by Incentive Support (0.143) and E-Commerce Cluster (0.131). Based on these analyses, the main implications of this study are, first, in the current situation where the cross-border e-commerce market is growing, Pyeongtaek Port needs to form a consultative body among the government, local governments, and related businesses in connection with cross-border e-commerce and develop various support policies for the e-commerce market. Second, it will have to be able to provide differentiated services from competing ports by establishing e-commerce market-oriented clusters. Originality/value - In existing related studies, various improvements were presented to revitalize trade in line with the growth of the cross-border e-commerce market. However, with regard to most cross-border e-commerce businesses, one-dimensional improvement measures, such as improvement of payment systems, improvement of customs clearance services, and promotion of human resources, are presented in a piecemeal manner. In other words, none of the studies have proposed the importance and priority of each measure in terms of both the forward-looking and efficient allocation of resources, which is the purpose of this study. Therefore, this study contributed politically, practically, and academically by presenting countermeasures for ports to revitalize cross-border e-commerce and presenting strategic priorities using quantitative analysis methods.

Intellectual structure and research trends of The Research Journal of the Costume Culture - Bibliometric quantitative and qualitative semantic network approaches - (<복식문화연구>의 지적구조와 연구동향 - 계량정보학적 양적 접근과 의미연결망의 질적 접근 -)

  • Choi, Yeong-Hyeon;Choi, Mi-Hwa
    • The Research Journal of the Costume Culture
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    • v.30 no.4
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    • pp.608-630
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    • 2022
  • The purpose of this study is to examine the relationships between citations and the research trends of The Research Journal of the Costume Culture (RJCC) using bibliometric and network analyses. The results are as follows. First, the RJCC has been cited by a greater number of journals and high-reputation journals today. The RJCC has been mentioned in global academic journals in various fields, and it has been noted the most in environmental science. Second, because of examining the articles published in the RJCC over the past three years (2019 - 2021), it was found that the number of topics was evenly distributed in various subfields of the clothing and textiles sector. The RJCC principally deals with traditional clothing, ethics and sustainability, and technology, which means that the RJCC reflects the past, present, and future. As a result of conducting a cluster analysis using the Wakita-Tsurumi algorithm, the subjects of ethical fashion and sustainability were derived from the subdivisions of the RJCC. This suggests that the RJCC is a journal specialized in ethical fashion and sustainability sectors such as environmental, animal, and labor ethics. This study outlined the current status and future direction of academic journals in the field of clothing through an analysis of the RJCC's influence change and the relationship between citations. In addition, it is academically significant because it identifies research trends and knowledge-structure changes in the apparel science field by identifying changes in research keywords and significant research topics by sector.