• Applied Biological Chemistry
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• 제40권6호
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• pp.495-500
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• 1997

Cyclodextrin을 합성하는 효소 CGTase를 호염기성 Bacillus sp. E1으로부터 분리하기 위하여 PCR을 실시하였다. PCR을 위하여 합성한 primer의 염기서열은 현재까지 보고된 CGTase 유전자의 염기서열을 비교 분석하여 가장 높게 보존된 영역을 찾아내어 선택하였다. PCR 증폭 결과 1.2 kbp 크기의 DNA 절편을 얻을 수 있었고 이를 molecular probe로 이용하여 Southern blot 분석을 실시하였다. Southern blot 분석결과 CGTase 유전자는 염색체 DNA를 제한효소 XbaI으로 절단한 5.3 kbp 절편내에 존재한다는 사실을 알아내었다. CGTase 유전자를 분리하기 위하여 유전자 은행을 제조한 후 선별작업을 실시하여 genomic clone인 pCGTE1을 얻을 수 있었다. pCGTEl의 염기서열을 결정한 결과 분리한 CGTase 유전자는 2109 bp의 open reading frame을 가지며 이는 703개의 아미노산으로 구성된 단백질을 coding하는 것으로 나타났다. 아미노산 서열의 유사성을 비교한 결과 Bacillus sp. KC201의 CGTase 와 가장 높은 94.3% 동질성을 나타내었다.

• 한국토양비료학회지
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• 제33권3호
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• pp.153-159
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• 2000

토성분석방법으로 사용되고 있는 표준비중계법 (Standard Method ; SM)과 개선비중계법(Simplified for Soil Texture Analysis ; SSTA)및 간이계산법(Simplified Clay Fraction Piocedure;SCFP)간의 $2{\mu}m$ 점토함량을 비교하고자 본 시험을 수행하였다. 사용된 시료는 점토함량이 2~40% 내외의 토양 28점이었다. 현재 국내사용 표준방법은 particle size Distribution을 위한 것이지, 토성판정을 위한 $2{\mu}m$ 점토함량을 계산하는데는 약간의 문제가 있는 것으로 판단되었으며 표준분석법과의 상관을 볼 때는 개선비중계법과 간이계산법 모두 1.0에 가까운 상관계수를 보였으나, 간이계산법의 경우 표준분석 법보다는 $2{\mu}m$ 점토함량이 0~2%정도 높게 평가 되었다. 또한 분석한 시료의 토성판정결과 표준분석법과 개선비중계법은 28점 모두 동일한 결과를 얻었으나, 표준분석법과 비교할 때 간이계산법은 28점중 4점이 다른 토성으로 판정되었다. 그러므로 표준분석법 방법보다는 개선비중계법 방법이 $2{\mu}m$ 점토함량을 측정하는데 더 신속하고 정확한 방법이 될 수 있을 것으로 판단되었다.

• 인문언어
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• 제2권2호
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• pp.85-107
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• 2002

The word play on h(e)art-hunting has become a virtual commonplace in criticism of Chaucer′s Book of the Duchess. Less widely discussed is the third meaning of ME herte, "hurt." The "hart"/ "heart" pun is, however, only implicit in the poem, while the rhyme of "heart" and "hurt" in lines 883-84 makes clear the close association of the terms for Chaucer. Earlier commentators insisted that this was in fact an instance of rime riche or "identical rhyme," but if it is so it is striking that it is the unique instance of the rhyme in Chaucer, whose works are full of occasions for hurt hearts. The essay argues that this is, instead, an instance of near-rhyme and that the confusion in scribal spellings of ME hurten(with ′u,′ ′0,′ ′i,′ ′y,′ and ′e′ ) suggests uncertainties about its root vowel that modem linguistic study has not clarified completely. If the rhyme of herte ("hurt") with herte ("heart") is, however, established by these lines in BD, then it is probably reasonable to ask about all the occasions where characters in the poem are hurt by emotional or physical distress. In the cases of A1cyone and the Man in Blak, the hurt is revealed plainly as the death of a loved one, and Alcyone′s death and the Man in Blak′s return "homwarde" offer contrasting responses to the realization and acknowledgement of their loss. In the case of the Narrator, however, the exact nature of his "hurt" is nowhere made clear and the questions this Jack of clarity raises for the reader remain unanswered when the poem declares its "hert-huntyng" done. Further examination of the Narrator′s character and his role in the poem may reveal him to be a physician himself in need of healing, and this reading of his character may identify him as an ancestor as much of Chaucer′s Pardoner as of the Pilgrim Narrator of Canterbury Tales.

• 건축역사연구
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• 제7권3호
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• pp.151-170
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• 1998

This study is about Le Corbusier's early years of loarning and training at La Chaux-de-Fonds. It is an attempt to show how Le Corbusier's groundwork was laid which characterize his work throughout the life. Charles Edouard Jeanneret was trained as a watch engraver and wished to be a painter. C. L'Eplattenier was to play a decisive role in shaping the young introspective boy's future. He encouraged Jeanneret's habit of the close study and observation of nature. Jeanneret was not the product of an established school, but instead made the unusual choice of educating himself. He found two indispensible sources of inspiration in study the past and in contemplating nature. His four years of self-education consisted of extensive reading, summer travels and winter layovers in larger cities-Vienna, Paris, Berlin-while sketching in museums or apprenticing local architects-Pellet, Behrens-. All these impressions then blended together to become part of a comprehensive source book of knowledge and imagination of the later Le Corbusier. A largely self-taught man, he never stopped making notes, drawing and writing, always aspiring to a clearer understanding of the meaning and underlying principles of objects and architecture. Jeanneret's five villas in La Chaux-de-Fonds are barometers which show the sequences of his development and change as an architect. In 1917, being thirty, he uprooted himself from his hometown to get a wider range of opportunities and moved to Paris. By that time Jeanneret was almost ready to blossom into Le Corbusier It was during this formative years of his life that Le Corbusier established the working method, mind-set and philosophical basis that determined the course of an architect in the making.

• 한국항행학회논문지
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• 제17권1호
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• pp.39-46
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• 2013

비정상적인 시스템 종료로 인해 파일 시스템이 손상되었을 경우 시스템은 fsch을 이용하여 일관성 검사를 수행하며 이는 오랜 시간이 소요된다. 특히 대용량의 파일 시스템인 경우에는 상당한 시간이 걸린다. 저널링 기법을 이용한 저널링 파일 시스템은 메타 데이터를 이용하기 때문에 복구 시간을 상당히 단축시킬 수 있으며, 복구시 복구 확률도 높일 수 있다. 본 논문의 목적은 현재 리눅스에서 사용되고 있는 저널링 파일 시스템을 커널 기반의 벤치 마킹 도구인 Iozone을 사용하여 파일의 read, write 중심으로 성능을 비교평가하는 것이다. 본 논문에서는 현재 리눅스의 기본 파일 시스템인 Ext4 파일 시스템이 파일 읽기 성능이 경우 XFS 파일 시스템보다 1.28배, 파일의 쓰기 성능의 경우 Ext3 보다 1.22배 빠르게 전송되었다.

• 한국전자파학회논문지
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• 제20권9호
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• pp.823-828
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• 2009

열쇠 관리용 철재 캐비닛 안에 장착되는 UHF 대역의 열쇠고리형 소형 태그 안테나를 설계 제작하였다. 열쇠 고리형 태그 안테나는 인접한 태그 안테나, 열쇠고리의 금속 부분, 그리고 캐비닛 등과의 상호 간섭 작용을 고려하여 소형으로($66\;mm{\times}6\;mm$) 제작하였다. 제작된 태그 안테나는 T-matching 방법을 사용하여 정합하였고, 미엔더(Meander) 형태를 이용하여 크기를 줄여서 유전체(FR-4) 기판을 사용하여 제작하였다. 먼저 인접한 태그 안테나들과의 상호 간섭을 감안하여 태그 안테나를 설계하였고, 캐비닛의 철재 근처에 장착되는 태그 안테나용으로 또 다른 설계를 제안하였다. 인접한 태그 안테나들의 개수를 변화시켜가며 태그 안테나의 특성과 인식 거리에 미치는 영향을 분석하고 측정하였다. 그 결과 캐비닛에 장착되는 총 360개의 태그 안테나를 모두 인식할 수 있었다.

• 한국방사선학회논문지
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• 제11권2호
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• pp.69-77
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• 2017

본 연구는 영상의 노이즈 감소를 위한 복합필터 제안과 간 초음파영상에서 노이즈 제거를 통한 영상품질의 향상으로 정확한 영상 해석에 도움을 주고자 하였다. 실험은 ATS-539 다목적용 초음파팬텀을 이용하여 Gaussian blurring filter, Sharpening filter, Median filter의 조합으로 상위 7개의 복합필터를 선정하고 이를 간 초음파영상에 적용하였다. 그 결과 상위 7개 복합필터 적용하였을 때 SNR, CNR, MSR 값이 모두 증가하는 것으로 나타났다. 이 중 복합필터 1과 2가 가장 높은 값을 나타내었다. 또한 PSNR 30 dB이상, SSIM 1에 가까운 결과값을 나타내어 영상의 손실률이 적음을 알 수 있었다. 따라서 본 연구에서 제안한 복합필터의 적절한 적용은 정확한 영상의 판독과 해석에 도움을 줄 수 있을 것이다.

• 미생물학회지
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• 제38권4호
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• pp.230-230
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• 2002

Class Ⅲ chitin synthases in filamentous fungi are important for hyphal growth and differentiation of several filamentous fungi. A genomic clone containing the full gene encoding Chs4, a class Ⅲ chitin synthase in Penicillium chrysogenum, was cloned by PCR screening and colony hybridization from the genomic library. Nucleotide sequence analysis and transcript mapping of chs4 revealed an open reading frame (ORF) that consisted of 5 exons and 4 introns and encoded a putative protein of 915 amino acids. Nucleotide sequence analysis of the 5′flanking region of the ORF revealed a potential TATA box and several binding sites for transcription activators. The putative transcription initiation site at -716 position was identified by primer extension and the expression of the chs4 during the vegetative growth was confirmed by Northern blot analysis. Amino acid sequence analysis of the Chs4 revealed at least 5 transmembrane helices and several sites for past-transnational modifications. Comparison of the amino acid sequence of Chs4 with those of other fungi showed a close relationship between P chrysogenum and genus Aspergillus.

• Journal of Microbiology and Biotechnology
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• 제27권11호
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• pp.1983-1993
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• 2017

Salmonella enterica subsp. enterica serovar Typhimurium, one of the most common foodborne pathogens, is transmitted mainly through contaminated food derived from infected animals. In this study, S. Typhimurium ST1120, an isolate from pig feces in Korea, was subjected to whole-genome analysis to understand its genomic features associated with virulence. The genome of ST1120 was found to have a circular chromosome of 4,855,001 bp (GC content 52.2%) and a plasmid of 6,863 bp (GC content 46.0%). This chromosome was predicted to have 4,558 open reading frames (ORFs), 17 pseudogenes, 22 rRNA genes, and 86 tRNA genes. Its plasmid was predicted to have three ORFs. Comparative genome analysis revealed that ST1120 was phylogenetically close to S. Typhimurium U288, a critical isolate in piggery farms and food chains in Europe. In silico functional analysis predicted that the ST1120 genome harbored multiple genes associated with virulence and stress resistance, including Salmonella pathogenicity islands (SPIs containing SPI-1 to SPI-5, SPI-13, and SPI-14), C63PI locus, ST104 prophage locus, and various antibiotic resistance genes. In accordance with these analysis results, ST1120 showed competence in invasion and survival abilities when it was added to host cells. It also exhibited robust resistance against antibiotics in comparison with other S. Typhimurium strains. This is the first report of the complete genome sequence of S. Typhimurium isolated from swine in Korea. Comparative genome analysis between ST1120 and other Salmonella strains would provide fruitful information toward understanding Salmonella host specificity and developing control measures against S. Typhimurium infection.

• Child Health Nursing Research
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• 제8권4호
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• pp.469-481
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• 2002

The purpose of this study was to explore the parents and child relationship in traditional Korean society through the Korean classic novels. The Data were analyzed through latent content analysis in focusing on the parents and child relationship. Forty-six Korean classic novels written in Chosun Dynasty (from 1392 to 1910 AD) were chosen to analyze and they were represented the characteristic Confucian ideas. The sentences containing the expression of child-care and parent-child relationship were selected during intensive reading line by line and analyzed. Sixty-nine meaningful concepts were drawn out from 258 significant sentences by the similar meaning and common theme and classified as 3 categories and 11 sub-categories. The important parent-child relationship expressed in Korean traditional society were as follows: From the category of the Meaning of Child, 4 sub-categories were drawn; gift of heaven, successor of the family line, driving power of life, and rewards from the offspring. From the category of attitude to the child, 3 sub-categories were drawn; a notion of preferring a son to a daughter, close maternal-child relation, and expectation to the future of child. From the category of nurturing behavior, 4 subjects were drawn; Tae-Gyo(fetal education), affective behavior, socialization, and health management behavior. Above result helps to improve the basic understanding the relationship of parent and child in modern society. And by the understanding of child in the family, unhealthy relationship of parent and child can be prevented and furthermore family centered child health promotion can be achieved.