• 한국동물위생학회지
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• 제35권1호
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• pp.1-8
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• 2012

To detect the virulence genes (invA and spvC) and antimicrobial resistance genes, polymerase chain reaction (PCR) was carried out using total 67 strains of S. Schwarzengrund isolated from pigs. As results, invA was detected from all 67 strains of S. Schwarzengrund, however, spvC was not at all. All 12 strains with ampicillin resistance, 15 strains with chloramphenicol resistance, 9 strains with kanamycin resistance, 1 strain with sulfamethoxazole/trimethoprim resistance, and 66 (98.5%) of 67 strains with tetracycline resistance carried TEM (${\beta}$-lactamase $bla_{TEM}$), cmlA (nonenzymatic chloramphenicol resistance), aphA1-Iab (aminoglycoside phosphotransferase), sulII (dihydropteroate synthase), and tetA (class A tetracycline resistance), respectively. All 63 strains with streptomycin resistance carried 3 aminoglycoside resistance genes, including aadA (aminoglycoside adenyltransferase), strA, and strB (streptomycin phosphotransferase). With respect to prevalence of antibiotic resistance genes occurred in S. Schwarzengrund, genes for strB (46.0%); strA and strB (30.2%); aadA, strA, and strB (9.5%); strA (7.9%); aadA and strB (3.2%); and aadA (3.2%) were detected by PCR.

• Restorative Dentistry and Endodontics
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• 제22권1호
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• pp.447-463
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• 1997

The purpose of this study was to evaluate the effectiveness of copal varnish and five bonding agents(Scotchbond Multi-Purpose Plus Dental Adhesive System, Panavia 21, All Bond 2, Superbond D-Liner II plus, Fuji Duet) in reducing microleakage under amalgam restorations. Class V cavity were prepared on both buccal and lingual surface of sixty extracted human molars with cementum margin and were filled with different kinds of liners and amalgam. All teeth were stored at $37^{\circ}C$ in physiologic saline solution. After 1 week they were thermally stressed for 500 cycles between 5 and $55^{\circ}C$ in baths containing 0.5 % basic fuchsin dye. The dye penetrations were observed with a stereomicroscope. Scanning electron micrographs were taken of representative tooth sections from each group to assess the nature of the tooth/liner/amalgam interface. The statistical test were applied to the results using a one way analysis variance (ANOVA) and Duncan's multiple range test. The results were as follows ; 1. In all groups, the enamel margin showed significantly lower leakage value than the cementum margin (p<0.05). 2. At the dentin and enamel margins, the leakage value of Copalite-lined group showed significantly higher than that of no liner group (p<0.05), but showed significantly lower than that of bonding agent lined-groups (p<0.05). 3. There was no significant difference between the bonding agent lined-groups (p>0.05). 4. On the backscattered scanning electron microscopy observation, discontinuous gaps were observed between amalgam and dentin in the bonding agent-lined amalgam restorations and the bonding agents appeared to fill the gap space and were mixed with amalgam particles. At the amalgam/tooth interface, unsealed dentin and continuous gaps were found in both unlined and Copalite-lined restorations.

• 한국수정란이식학회지
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• 제33권2호
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• pp.85-97
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• 2018

The trans-differentiation potential of mesenchymal stem cells (MSCs) is employed, but there is little understanding of the cell source-dependent trans-differentiation potential of MSCs into corneal epithelial cells. In the present study, we induced trans-differentiation of MSCs derived from umbilical cord matrix (UCM-MSCs) and from dental tissue (D-MSCs), and we comparatively evaluated the in vitro trans-differentiation properties of both MSCs into corneal epithelial-like cells. Specific cell surface markers of MSC (CD44, CD73, CD90, and CD105) were detected in both UCM-MSCs and D-MSCs, but MHCII and CD119 were significantly lower (P < 0.05) in UCM-MSCs than in D-MSCs. In UCM-MSCs, not only expression levels of Oct3/4 and Nanog but also proliferation ability were significantly higher (P < 0.05) than in D-MSCs. In vitro differentiation abilities into adipocytes and osteocytes were confirmed for both MSCs. UCM-MSCs and D-MSCs were successfully trans-differentiated into corneal epithelial cells, and expression of lineage-specific markers (Cytokeratin-3, -8, and -12) were confirmed in both MSCs using immunofluorescence staining and qRT-PCR analysis. In particular, the differentiation capacity of UCM-MSCs into corneal epithelial cells was significantly higher (P < 0.05) than that of D-MSCs. In conclusion, UCM-MSCs have higher differentiation potential into corneal epithelial-like cells and have lower expression of CD119 and MHC class II than D-MSCs, which makes them a better source for the treatment of corneal opacity.

• Genomics & Informatics
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• 제13권4호
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• pp.126-131
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• 2015

Fulminant type 1 diabetes (T1DM) is a distinct subtype of T1DM that is characterized by rapid onset hyperglycemia, ketoacidosis, absolute insulin deficiency, and near normal levels of glycated hemoglobin at initial presentation. Although it has been reported that class II human leukocyte antigen (HLA) genotype is associated with fulminant T1DM, the genetic predisposition is not fully understood. In this study we investigated the HLA genotype and haplotype in 11 Korean cases of fulminant T1DM using imputation of whole exome sequencing data and compared its frequencies with 413 participants of the Korean Reference Panel. The $HLA-DRB1^*04:05-HLA-DQB1^*04:01$ haplotype was significantly associated with increased risk of fulminant T1DM in Fisher's exact test (odds ratio [OR], 4.11; 95% confidence interval [CI], 1.56 to 10.86; p = 0.009). A histidine residue at $HLA-DR{\beta}1$ position 13 was marginally associated with increased risk of fulminant T1DM (OR, 2.45; 95% CI, 1.01 to 5.94; p = 0.054). Although we had limited statistical power, we provide evidence that HLA haplotype and amino acid change can be a genetic risk factor of fulminant T1DM in Koreans. Further large-scale research is required to confirm these findings.

• Imaging Science in Dentistry
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• 제41권4호
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• pp.143-150
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• 2011

Purpose : The aim of this study was to compare the diagnostic accuracy of cone-beam computed tomography (CBCT) images and bitewing images in detection of secondary caries. Materials and Methods : One hundred and twenty proximal slots of Class II cavities were randomly prepared on human premolar and molar teeth, and restored with amalgam (n=60) and composite resin (n=60). Then, artificial secondary caries lesions were randomly created using round steel No. 4 bur. The teeth were radiographed with a conventional bitewing technique and two CBCT systems; Pax-500ECT and Promax 3D. All images were evaluated by five observers. The area under the receiver operating characteristic (ROC) curve ($A_z$) was used to evaluate the diagnostic accuracy. Significant difference was tested using the Friedman test (p value<0.05). Results : The mean $A_z$ values for bitewing, Pax-500ECT, and Promax 3D imaging systems were 0.882, 0.995, and 0.978, respectively. Significant differences were found between the two CBCT systems and film (p=0.007). For CBCT systems, the axial plane showed the greatest $A_z$ value. Conclusion : Based on the design of this study, CBCT images were better than bitewing radiographs in detection of secondary caries.

• 대한소아치과학회지
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• 제25권2호
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• pp.323-334
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• 1998

The purpose of this study was to compare the microleakage of the amalgam and the gallium alloy when several lining materials were applied. The ANA 2000, high copper lathecut type amalgam(AM group) and Gallium Alloy GF II (GF group) were used. The fifty extracted sound molars were randomly assigned to AM group and GF group, and the buccal and lingual class V cavities with a size $3{\times}2{\times}2mm$ were prepared. The prepared cavities were randomly assigned to group 1 : no liner used control, group 2 : Copalite, group 3 : Panavia 21, group 4 : All-bond 2, and group 5 : Superbond C&B. After liner placement and amalgam filling, the specimens were stored in $37^{\circ}C$ normal saline for 24 hours and then thermocycled from $5^{\circ}C$ to $55^{\circ}C$ thousand times. The specimens were stored in the 1% methylene blue solution for 24 hours and sectioned and examined by stereomicroscope. The results obtained from this study can be summarized as follows : 1. In the GF group, microleakage values of group 2, 3, 4, 5 were significantly lower than that of group 1 (p<0.05). 2. In the AM group, microleakage values of group 3 and 4 were significantly lower than that of group 1 (p<0.05), but microleakage values of group 2 and 5 did not differ from that of group 1 (p>0.05). 3. The GF group was similar(group, 1 3, 4) or superior(group 2, 5) to the AM group in the aspect of the microleakage.

• 구강회복응용과학지
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• 제30권2호
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• pp.192-198
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• 2014

본 증례는 심미적인 개선을 위해 내원한 30대 여성에서 도재 라미네이트 비니어(porcelain laminate veneer: PLV)와 전 부도재관을 이용하여 치료를 시행한 증례로서, 환자는 앞니가 튀어나오고 고르지 않다는 주소로 내원하였고 최소의 치아 삭제를 원하였다. 환자는 골격성 II급 부정교합이었으며, 진단 납형 제작 시 이를 고려하기 위해 체크 바이트법을 이용하여 교합기에 마운팅 하였고, 진단 납형 제작한 상태의 mock-up을 이용하여 치아 삭제를 시행한 후 삭제량을 인덱스로 확인하였다. 보철물의 전체적인 색조의 조화를 위해 도재 라미네이트 비니어를 먼저 수복하였고, 전부도재관의 지대치 색상을 고려한 뒤 수복하여 만족스러운 심미성의 개선을 얻을 수 있었다.

• 대한소아치과학회지
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• 제45권1호
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• pp.21-31
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• 2018

본 연구는 7세에서 11세 사이의 혼합치열기 아동의 전치 경사도와 입술 경사도 사이의 연관성을 알아보고자 하였다. 353명의 측모두부방사선 사진을 분석하여, 골격성 부정교합의 분류에 따른 상악 전치 경사도, 하악 전치 경사도, 하-비순각, 이순각을 평가하였다. 측정된 수치는 Kruskal-Wallis test와 피어슨 상관분석 및 다중회귀분석을 통해 평가하였다. 모든 골격성 부정교합에서 상악 전치 경사도와 상순 경사도 사이에는 유의한 음의 상관관계가 확인되었고, 하악 전치 경사도와 하순 경사도 사이에는 음의 상관관계가 확인되었으나 2급 부정교합에서는 유의할만한 양의 상관관계가 확인되었다. 본 연구를 통해 입술 경사도에 영향을 미치는 요인들을 확인하고 그 연관성을 확인할 수 있었다.

• 대한치과교정학회지
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• 제39권1호
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• pp.54-65
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• 2009

본 증례보고는 성인형 치주염으로 인해 전반적인 치조골 소실을 보이고 양악 전돌을 동반한 II급 부정교합으로 진단된 50세 10개월 된 여자환자의 치료를 소개하고자 한다. 치주 치료를 진행한 후 양악 전돌을 해소하기 위해 양악 제1소구치를 발치하고, 상악 전치부는 피질골 절단술 시행 후 악정형적 견인을, 하악 전치부는 6전치의 전방부 분절골 절단술[Anterior segment osteotomy(ASO)]을 국소마취하에 시행하였다. 총 치료기간은 9개월이 소요되었고 안정적인 교합관계와 안모의 개선이 이루어졌다. 하지만 치료 후에 하악 전치부에 약간의 치근 흡수 소견이 관찰되었다. 치료 27개월 후에도 안정적인 치료결과가 유지되었다.

• 대한치과교정학회지
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• 제48권3호
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• pp.189-199
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• 2018

For patients with bimaxillary protrusion, significant retraction and intrusion of the anterior teeth are sometimes essential to improve the facial profile. However, severe root resorption of the maxillary incisors occasionally occurs after treatment because of various factors. For instance, it has been reported that approximation or invasion of the incisive canal by the anterior tooth roots during retraction may cause apical root damage. Thus, determination of the position of the maxillary incisors is key for orthodontic diagnosis and treatment planning in such cases. Cone-beam computed tomography (CBCT) may be useful for simulating the post-treatment position of the maxillary incisors and surrounding structures in order to ensure safe teeth movement. Here, we present a case of Class II malocclusion with bimaxillary protrusion, wherein apical root damage due to treatment was minimized by pretreatment evaluation of the anatomical structures and simulation of the maxillary central incisor movement using CBCT. Considerable retraction and intrusion of the maxillary incisors, which resulted in a significant improvement in the facial profile and smile, were achieved without severe root resorption. Our findings suggest that CBCT-based diagnosis and treatment simulation may facilitate safe and dynamic orthodontic tooth movement, particularly in patients requiring maximum anterior tooth retraction.