• Title/Summary/Keyword: citrus juice

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Optimization of Culture Condition of Gluconacetobacter hansenii TF-2 for Cellulose Gel Production (Gluconacetobacter hansenii TF-2를 이용한 감귤과즙으로부터의 셀루로스 겔 생산의 최적화)

  • 최경호;정지숙;문철호;김미림
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.1
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    • pp.176-181
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    • 2004
  • Gluconacetobacter hansenii TF-2, an isolate from black tea fungus, was statically cultivated to ferment cellulose gel from citrus juice. The juice prepared by press filtering of peeled citrus fruit contained 135.5 mg of total sugar/mL, 1.23% of total acid, and average pH of the juice was 3.98. The bacterium produced cellulose gel optimally on the surface of culture broth containing 17% of citrus juice and 10$^{\circ}$Brix of total sugar. The optimum temperature was 3$0^{\circ}C$ for producing acetic acid and gel formation. The bacterium could not produce acetic acid on gel formation at 4$0^{\circ}C$. The optimum pH was 3.0∼4.0 but was not significantly different between pH 3.0∼4.0. The cultivation for 18 days under optimal conditions produced gel as 14.2$\pm$0.6 mm of thickness and acids equivalent to 1.90$\pm$0.22% of acetic acid. The pH of culture broth was stabilized at 2.6∼2.8 during the cultivation. Remaining sugar content was 27.1$\pm$4.2 mg/mL of total sugar and 6.9 mg/mL of reducing sugar. The gel productivity was 137.8$\pm$9.7 g/L.

Development of Fermented Beverage Using the Sea Tangle Extract, and Quality Characteristics Thereof (다시마추출액을 이용한 발효음료개발 및 품질특성)

  • Kim, Mi-Lim;Choi, Mi-Ae;Jeong, Ji-Suk
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.21-29
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    • 2008
  • This study investigated the fermentation and quality characteristics of a fermented beverage, prepared by semi-anaerobic culture, using sea tangle extract. A central composit design using alcohol(0, 0.5, 1.0, 1.5, 2.0% [all v/v] ), sugar(0, 5, 10, 15, 20% [all w/v] ) and $65^{\circ}Brix$ citrus juice(0, 1.0, 2.0, 3.0, 4.0 % [all v/v] ) was used to find the optimal mix for fermentation. Sensory characteristics, such as color, flavor, taste, sweetness, saltiness, sourness and overall quality, were measured using a response surface methodology computer program. The optimal conditions that produced the highest acidity of 0.94 were 2.0 % ethanol, 10.17 % sucrose and 1.99 % citrus juice. The optimal conditions that produced gel 20.13 nun in thickness were 1.98% alcohol, 10.94% sucrose and 1.62% citrus juice. The overall optimal conditions that satisfied all the sensory requirements for a sea tangle beverage were 1.0% alcohol, 10.0% sucrose and 4.05% citrus juice.

Deacidification of Mandarin Orange Juice by Electrodialysis Combined with Ultrafiltration

  • Kang, Yeung-Joo;Rhee, Khee-Choon
    • Preventive Nutrition and Food Science
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    • v.7 no.4
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    • pp.411-416
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    • 2002
  • The effects of electrodialysis (ED) alone or ED plus ultrafiltration (UF) on deacidification of mandarin orange juice were studied by using a commercial ED stack with ion exchange membranes. ED processing, reduced the total acidity of the juices by 30% (0.6~0.7% as total acidity) after 50 min and by about 60~70% (0.23~0.4% as total acidity) after 100 min, as compared to the control juice. However, the acidity reduction after 50 min of ED was determined to be suitable, when considering total acidity (0.6~0.7%, w/w) and current efficiency. There was no color change in the juices following ED, and the pH and Brix were only slightly decreased. Furthermore, ascorbic acid and citric acid concentrations showed only minor decreases, and amino-N, free sugar, and flavonoid contents remained almost unchanged. Therefore, we concluded that the nutritional integrity of the juice was maintained. ED combined with UF may be effective, not only in preventing membrane fouling, but also in preserving the nutrients, such as ascorbic acid, in citrus juice.

Characteristics of 'Hongrou Taoye', a Grafted Chimera in Sweet Orange and Satsuma Mandarin

  • Zhang, Min;Xie, Zongzhou;Deng, Xiuxin;Liao, Shengcai;Song, Wenhua;Tan, Yong
    • Horticultural Science & Technology
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    • v.33 no.3
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    • pp.390-395
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    • 2015
  • The synthesis of chimeras is a breeding approach for horticultural crops. In our breeding program, a new diploid citrus chimera, named 'Hongrou Taoye' (Citrus sinensis [L.] Osbeck + Citrus unshiu Marc.), was found arising at the junction where a 'Taoye' sweet orange (C. sinensis) scion was grafted onto Satsuma mandarin (C. unshiu). As an artificial chimera, its fruit traits derived from the L1 cell layer, with juice color and carotenoid complement, in which ${\beta}$-cryptoxanthin accumulated predominantly, similar to those of Satsuma mandarin. By contrast, traits originating from the L2/L3 cell layer, including pollen, seed, and rind aroma characteristics, were the same as those of 'Taoye' sweet orange (the scion). SSR and cpSSR analyses showed that both nuclear and chloroplast genomes of the chimera were a combination of both donor parents. 'Hongrou Taoye' thus combined the valuable traits of both donor plants, and therefore has good potential in citrus fresh market.

Carotenoid Pigment of Citrus Fruits Cultivated in Korea (한국산 감귤의 Carotenoid계 색소)

  • Whang, Hea-Jeng;Yoon, Kwang-Ro
    • Korean Journal of Food Science and Technology
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    • v.27 no.6
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    • pp.950-957
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    • 1995
  • The carotenoids of 14 Korean Citrus were analyzed by HPLC and spectrophotometry. Selected Citrus varieties were Satsuma mandarins, Orange, Ponkan, Marumeru and Iyeagam. The amount of carotenoid in peel and juice of Citrus is $0.82{\sim}10.64\;mg%$ and $0.23{\sim}3.38\;mg%$, respectively. Among the 14 peaks obtained from each samples, ${\alpha}-carotene$, ${\beta}-carotene$, Iycopene and lutein were identified by the direct comparison with authentics. Eigth peaks were assumed to neoxanthin, violaxanthin, antheraxanthin, lutein-5,6-epoxide, isolutein, cryptox-diepoxide, cryptox-5,6-epoxide and ${\beta}-cryptoxanthin$ through reference $t_R$ and two peaks remained unknown.

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Physicochemical Properties and Chemical Compositions of Citrus Fruits Produced in Cheju (제주산 감귤류 성분과 그 특성)

  • Kim, Seong-Hak;Koh, Jeong-Sam
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.541-545
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    • 1995
  • Physicochemical properties and chemical compositions of Citrus fruits produced in Cheju were investigated. Soluble solids, total sugar and acid content of Citrus unshiu were $10.4{\sim}11.0^{\circ},\;8.24{\sim}8.79%\;and\;1.04{\sim}1.20%$, respectively. Vitamin C ranging from $41.19{\sim}46.55\;mg/100\;g$ was higher on medium type than on early type of Citrus unshiu. In case of inorganic elements in Citrus unshiu, potassium content was the highest, in the range of $108.66{\sim}132.65\;mg/100\;g$, and it was higher on C. platymamma than on Citrus unshiu. however, the kinds and contents of carbohydrate in citrus juice were somewhat different among citrus varieties. Sucrose was $46.8{\sim}64.6%$ and others were glucose and fructose, $18.4{\sim}26.9%\;and\;15.2{\sim}30.2%$, respectively. Citric acid content was $75.7{\sim}96.2%$, and others were malic acid, oxalic acid, and fumaric acid in the decreasing order.

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Physicochemical Properties of Citrus Hallabong Granules (한라봉 감귤 과립의 물리화학적 특성)

  • Lee, Chung-Woo;Kim, Mi-Bo;Oh, Young-Ju;Lim, Sang-Bin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.537-543
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    • 2014
  • Three different granule powders of Citrus Hallabong were prepared with different ratios of fresh juice and citric acid (J10C3=10.0:3.08, J08C4=8.75:4.33, J07C5=7.5:5.58) at fixed quantities of juice powder (70%), pressed cake powder (10%), and additives (6.92%), and their physicochemical properties were measured and compared with targeted commercial product (CP). The moisture content of Hallabong granules (HG) was 5.08~5.44% (w/w), which was two times higher than that of CP. Titratable acidities increased with higher citric acid content and were 1.7~2.3 times lower in HG compared to that of CP. Soluble solids of HG increased with higher citrus juice content ($90{\sim}98^{\circ}Brix$) and were slightly lower than that of CP. Vitamin C contents of HG increased with higher citrus juice content and were 5.8~7.6 times lower than that of CP. In terms of color difference, L and b values of HG were higher while a value was lower than those of CP. Bulk and compaction densities of HG were 0.541~0.660 g/mL and 0.561~0.689 g/mL, respectively and were similar to those of CP. Water solubility and swelling capacity were 66.6~72.0% and 3.84~6.40 g/g, respectively, and were similar to those of CP. Hygroscopicity of HG after an hour of elapsed time was 1.6~1.8 times higher than that of CP. Sensory evaluation test showed that color, sweetness, and overall acceptance of HG were not significantly different from those of CP, whereas flavor and bitterness of HG were lower than those of CP. In conclusion, convenient food granules could be made with Citrus Hallabong juice and pressed cake, which is similar to the commercial product.

Analysis of Expression Pattern of the Limonoid UDP-glucosyltransferase Gene as an Indicator for Delayed Bitterness from the Citrus Species Endemic in Jeju (재래귤의 성숙시기별 리모노이드 쓴맛 표시자로서 limonoid UDP-glucosyltransferase 발현 분석)

  • Kim, Young-Mee;Lee, Do-Seung;Jeon, Deok-Hyoen;Song, Yeon-Woo;Lee, Dong-Sun;Ryu, Key-Zung;Cho, Moon-Jae;Lee, Dong-Hoon;KimCho, So-Mi
    • Food Science and Preservation
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    • v.18 no.2
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    • pp.184-190
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    • 2011
  • Limonoid UDP-glucosyltransferase (LUGT) is an enzyme that converts limonoids into their corresponding glucosides and ultimately ameliorates limonoid bitterness in Citrus species. In this paper, the LUGT gene was cloned via PCR from 10 Jeju Citrus species. All the deduced glucosyltransferase proteins harbored a highly conserved plant secondary product glucosyltransferase (PSPG) motif within the C terminal region. Phylogenetic analysis based on the amino acid sequence comparison of the LUGT proteins from 10 Citrus species generated three distinct types. The expression patterns of LUGT gene in three representative species from each type were quite different with that of C. unshiu Marc. cv. Miyagawawase(Gungcheon), which his without distinctive juice delayed bitterness. Ourresultssho wth at some Citrus speciessuchas Citrusleiocarpa HORT(Bingul), Citruserythrosa HORT (Dongjunggul), and Citrustachibana TANAKA(Honggul) end emicin Jeju maybe susceptible to intense juice delayed bitterness due to delay inexpression of LUGT.

Changes of Components of Citrus Sudachi Juice Heated at Various Temperatures (가열온도에 따른 영귤 과즙의 성분 변화)

  • Kim, Young-Dong;Lee, Young-Chul;Oh, Young-Ju;Kang, Young-Joo
    • Korean Journal of Food Science and Technology
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    • v.33 no.2
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    • pp.238-244
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    • 2001
  • The study was performed to investigate the changes of components and volatiles in citrus sudachi juice heated at 40, 50, 60, 70, 80 and $90^{\circ}C$. Total acidity, $^{\circ}Brix$, pH, organic acids, free amino acids, vitamin C, naringin, hesperidine, neohesperidin and volatiles were analyzed in fresh and heated citrus sudachi juices. The major organic acids were citric, malic and oxalic acids and their total contents were 5.27-5.48%. Citric acid content exceeded 92%, malic and oxalic acids were 3.6 and 3.2% in total orgainc acids. The organic acids decreased as heating temperature increased, but the their decreasing contents were 0.3% of total oraganic acids. Sixteen kinds of free amino acids presented in citrus sudachi juice. Major free amino acids were alanine, threonine, proline, aspargine, aspartic acid, serine, tyrosine, and trytophane and minor free amino acids were arginine, valine, glycine, lisoluecine, leucine and histidine. Free amino acids contents decreased as heating temperature increased. Vitamin C contents also decreased from 21.3 mg% to 17.3 mg% as heating temperature increased. Naringin, hesperidine and neohesperidin also slightly decreased from 304 mg% to 297.0 mg% as heating temperature increased. In the fresh and heated juices, a total of 50 volatiles were separated, of which 31 were identified. Limonene dominated in volatiles, followed by ${\gamma}-terpinene,\;{\alpha}-phellandrene$, myrcene and ${\alpha}-pinene$. ${\alpha}-Thujene$ presented in the fresh jucie but did not present in the heated juice above $50^{\circ}C$. However, ${\alpha}-Terpinolene$, terpinene-1-ol, ${\beta}-terpineol$, $cis-{\beta}-terpineol$, ${\alpha}-muurolene$, bicyclo(3.2.0)hept-6-ene, and mentha-1.4.8-triene did not presented in the fresh jucie but newly formed in the juice heated at $90^{\circ}C$.

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Quality Characteristics of Satuma Mandarin by Harvest Areas and Sea Level Altitude in Cheju (생산지역과 고도별 궁천조생 온주밀감의 품질특성)

  • 고정삼;강창희;좌창숙
    • Food Science and Preservation
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    • v.7 no.1
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    • pp.51-56
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    • 2000
  • Quality characteristics of Satsuma mandarin (Citrus unshiu Marc.var.miyagawa) harvest on early December according to production areas and sea level altitude of Cheju were investigated. Soluble solids and flesh ratio were distributed 9.32∼12.50, 75.95%-81.3 in citrus fruits harvested in orchards located below 10m sea level altitude (lower area), and was 10.23-12.17 , 77.35%-80.29% on those over 150m sea level altitude )higher area), respectively. Acid content of citrus juice was 0.95%-1.26% in fruits harvested inlower area, and was 1.02%-1.39% in higher area. In proportion to late harvesting , Brix/acid ratio and fruits quality was improved. Soluble solids of fruits harvested in higher area were higher than those of fruits from higher area, but acid contents were on the contrary . In sensory evaluation on fruit appearance, the differences between two altitudes were not clear.

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