• The Korean Journal of Mycology
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• v.16 no.3
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• pp.157-161
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• 1988

The vegetative nuclear divisions in hyphae and chromosome numbers were studied with the aid of Giemsa-HCl techniques from 10 strains of Fusarium oxysporum. The entire nuclear division process occurred within an intact nuclear envelope like other fungus. The results confirmed that 2 strains(F. oxysporum S Hongchun D2, F. oxysporum S Jinyang 4) were n=4; 3 strains(F. oxysporum f. sp. lini KFCC 32585, F. oxysporum f. sp. melongenae KFCC 34743 and F. oxysporum f. sp. raphani) n=5; 2 strains(F. oxysporum f. sp. vasinfectum, and F. oxysporum f. sp. mori KFCC 34742) n=6; 3 strains(F. oxysporum f. sp. cucumerium, F. oxysporum f. sp.niveum, and F. oxysporum f. sp. pisi) n=7.

• Korean Journal of Ichthyology
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• v.18 no.3
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• pp.155-162
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• 2006

The chromosome numbers of five species in two families of Korea are investigated: Odontobutis platycephala, O. interrupta, and O. obscura in Odontobutidae, and Cottus koreanus and C. hangiongensis in Cottidae. In Odontobutis species, the three species showed the diploid chromosome number, 2n=44 (NF=44) telocentric chromosomes. In Cottus species, the mitotic chromosomes from 24 groups with two chromosomes each indicated that it is a diploid. The karyotype of C. koreanus and C. hangiongensis is 2n=48 and NF=52. These species is the first report on the chromosomes and the karyotype analysis except O. platycephala. Our findings provide cytotaxonomic evidence for the species distinctness of these five species whose descriptions were based primarily on external morphology.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.4
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• pp.446-451
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• 1997

A Giemsa C-banding method was used for the identification of somatic chromosomes and heterochromatic knob positions in Korean indigenous maize(Zea mays L.). Total of 10 inbred stocks were examined and their knob numbers ranged from 6 to 12. In comparison of homologous chromosomes of two stocks of Waesungri and PI 213749, arm ratios and relative length of chromosomes were different between genotypes. In comparison of arm ratios, all the homologous chromosomes except chromosome 2 were different each other. In comparison of relative length of chromosomes, that of chromosome 1 in Waesungri and PI213749 was 223.22 and 192.03 respectively. The relative length of homologous chromosomes in Waesungri were generally lager than those of PI213749. A C-banded diagram showing knob positions, arm ratios and relative length of chromosome could be used as a good tool to compare the characteristics of chromosomes of Korean indigenous maize stocks.

• Korean Journal of Animal Reproduction
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• v.27 no.1
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• pp.53-59
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• 2003

For somatic cell nuclear transfer in Hanwoo, fetal fibroblast cell lines were established from 35, 50, 70 and 90-day fetuses of Korean native cattle. The sex of these fetal fibroblast cells were analyzed by PCR using Y-specific primers and confirmed that two cell lines were female and the other two cell lines were male. Karyotyping of these cell lines indicates that the chromosome numbers of fetal fibroblast cells were not affected by passage number and more than 80% of fetal fibroblast cells have normal chromosome number. To evaluate Go stage in cell cycle of fetal fibroblast cells, Western blotting was performed to detect the expression level of PCNA which is known to be expressed in all cell cycle stages except G$_{0}$ stage. Following serum starvation or confluent culture for 7 days, fetal fibroblast cells were effectively reached to G$_{0}$ stage. The cell cycle was resumed after culture of these Go stage-fetal fibroblast cells with normal medium. These results indicates that fetal fibroblast cells originated from Hanwoo were successfully isolated and culture system and induction of cell cycle of these cells were established for somatic cell nuclear transfer in Hanwoo.woo.

• Korean Journal of Ichthyology
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• v.1 no.1_2
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• pp.35-41
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• 1989

An examination of the karyotypes, DNA values and nuclear sizes of three scups was undertaken as part of the study of cytogenetical evolution of order Perciformes. The chromosome number 2n=48 was the same in all three species but the numbers of chromosome arm were not identical. The distribution of genome size and nuclear volumes among species was continuous ranging from 1.287 pg and $20.78\;{\mu}m^3$ for Pagrus major down to 1.237 pg and $20.56\;{\mu}m^3$ for Acanthopagrus schlegeli. Above results indicate the possible role of pericentric inversions in the karyotypic evolution of these species.

• Korean Journal of Plant Taxonomy
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• v.35 no.2
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• pp.99-114
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• 2005

Chromosome number, morphological variation and RAPD analysis were investigated to study on the speciation of Indigofera in Korea. Chromosome numbers of I. kirilowii (2n=16) and I. koreana (2n=32) are consistent with the previous reports. In this study tetraploid (2n=32) and hexaploid (2n=48) of I. grandiflora are newly observed. Indigofera grandiflora is distributed around Mt. Kaya area together with I. kirilowii and I. koreana. The former species has the larger sizes in plant height, leaves and flowers than the latter two and shows intermediate form between the two species in hairs on leaves and flowers which are one of the most important taxonomic characters in this group. In the RAPD analysis, I. grandiflora is similar to I. koreana than I. kirilowii but RAPD band patterns revealed difference between tetra- and hexaploid of the species. These results suggested that Korean endemic species of I. grandiflora (2n=16, 32, 48) might has multiple origin through polyploidization and/or hybridization between I. kirilowii (2n=16) and I. koreana (2n=32) around Mt. Kaya area where the latter two grow together.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.223-227
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• 2008

In rice, tillering is an important trait determining yield. To study tillering at the agricultural and molecular aspects, we have examined a spontaneous rice mutant that showed reduction in the number of culms. The mutant was derived from a $F^6$ line of the cross of Junambyeo*4 / IR72. It could produce, on average, 4 tillers per hill in the paddy field while wild-type plants usually have 15. Except the reduced culm numbers, they also show pale green phenotypes. The phenotypes of this mutant were co-segregated as the monogenic Mendelian ratio (${\chi}^b=0.002$, p=0.969). In order to locate a gene responsible for the rcn phenotype, the mutant with the japonica genetic background was crossed with Milyang21 of the indica background. Bulked segregant analysis was used for rapid determination of chromosomal location. Three SSR markers (RM551, RM8213, and RM16467) on chromosome 4 were genetically associated with the mutant phenotype. Each of the 217 $F_2$ plants was genotyped with simple sequence length polymorphisms. The data showed that RM16572 on chromosome 4 was the closest marker that showed perfect co-segregation among the $F_2$ population. We suggest the new rcn gene studied here name as $rcn10^t$ because there was no report which exhibit a rcn phenotype with a pleiotropic effect of pale green (chlorophyll deficiency), and mapped at same position on chromosome 4.

• Journal of Korean Society of Forest Science
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• v.80 no.4
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• pp.383-392
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• 1991

The genetic variation of Pinus densiflora and Pinus thunbergii by Giemsa C-banding was investigated and the results were as follows : 1. From Karyotype analysis of P. densiflora and P. thunbergii by Giemsa C-banding, somatic chromosome numbers of both species were 2n=24. 2. Chromosome of P. densiflora was M-type in arm ratio and they were no variation among individuals but variation in number and position of the secondary constriction and telomere banding among individuals. 3. P. thunbergii showed also M-type in arm ratio of chromosome, however, there was no variation in both number and position of the secondary constriction among individuals. 4. From chromosome C-banding, bands were appeared in the position of centromere and the secondary constriction in both P. densiflora and P. thunbergii. 5. In P. densiflora, the bands were shown on the secondary-constriction in chromosome No. 3, 4 and 7 of all individuals and the bands of the secondary constriction in chromosome No. 1, 2 and 5 showed variation among individuals. In chromosome No. 9, 10 and 11, the bands were shown in telomere and showed variation among individuals. 6. In P. thunbergii, the bands were shown on the secondary constriction in chromosome No. 2, 3, 7 and 8, and were shown no variation among individuals. There was no band on telomere. 7. The genetic variation by C-banding were shown in P. densiflora among individuals but no in P. thunbergii, and were shown on the secondary constriction in chromosome No. 4 of Pinus densiflora and in clnromosome No. 8 of Pinus thunbergii. These are the difference between the two species by C-banding.

• Journal of Life Science
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• v.18 no.7
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• pp.1019-1022
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• 2008

The chromosome numbers and compositions were investigated in four cultivated species of Cucurbitaceae; Cucumis sativus L., Citrullus lanatus (Thunb.) Matsum. et Nakai, Cucumis melo L., Luffa cylindrica (L.) Roemer. through general aceto-orcein staining method. The chromosome compositions of four species were diploids of 2n=22, 2n=24 and 2n=26 respectively. The chromosomes were relatively small and showed gradual length degradation from $2.50\;{\mu}m$ to $2.16\;{\mu}m$ in Cucumis sativus, $3.71\;{\mu}m$ to $2.11\;{\mu}m$ in Cucumis melo, $3.20\;{\mu}m$ to $2.40\;{\mu}m$ in Citrullus lanatus and $3.17\;{\mu}m$ to $1.97\;{\mu}m$ in Luffa cylindrica. The chromosome types consisted of all metacentrics in Cucumis sativus, seven pairs submetacentrics and five pairs metacertrics in C. melo, four pairs of submetacentrics and seven pairs metacertrics in Citrullus lanatus, and two pairs submetacentrics and eleven pairs metacentrics in Luffa cylindrica (L.) Roemer.. The satellites were found in a pair of chromosomes in C. melo and two pairs in Luffa cylindrica. The chromosome compositions in these four species showed species-specific patterns and seemed to provide useful informations for breeding and molecular cytogenetic works on Cucurbitaceae.

• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.1-12
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• 2003

The present study was carried out to establish the standard karyotype of the Korean Native Chicken and to find their chromosomal band markers using high-resolution banding technique. Chromosome analysis was performed on early chick embryos following in vitro culture of fertilized eggs of the yellow-brown and the red-brown lines of the Korean Native Chicken which had been established at National Livestock Research Institute. The high-resolution banding of the chromosome was achieved by treating the embryos with ethidium bromide and colchicine during culture. On GTG-banding, the Korean Native Chicken exhibited a typical chick banding pattern in all the macrochromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Chicken were virtually identical to those of White Leghorn and International System for Standardized Avian Karyotypes(ISSAK). However, the lengths and G-band numbers of the Korean Native Chicken macrochromosomes were greater than those of White Leghorn and ISSAK. Especially in chromosomes 1 and Z, the Korean Native Chicken exhibited more separated bands in compared with ISSAK. In C-banding patterns, although a lot of observed cells had C-band polymorphic patterns, almost the Korean Native Chicken macrochromosomes had heterochromatic C-band on centromeres and/or near terminal part. However, the heterochromatic C-band was constantly observed at the end of q-arm of Z chromosomes and on the whole W chromosome. In addition, the Korean Native Chicken exhibited distinctive heteromorphic patterns of C-bands on the centromere of chromosome 3 and at the end of q-arm of Z chromosome between homologous chromosomes.