• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.169-172
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• 2000

Studies on extraction of red pigment was performed to provide the basic information for the utilization of red pigment as s new source of natural food colorant. A bacterium isolated from marine resources were carried out the test for excretion of red pigment. One strain of a marine bacterium, KSR-97 showed a high production of red pigment on the medium of colloidal chitin, peptone-yeast extract with minerals. In physicochemical and sensory properties in aqueous solution of red pigment was investigated at various condition of pH, temperature, concentration of ethanol and stability of storage. Potent antioxidative of red pigment was separated by thin layer chromatograpy, silica gel chromatography and reverse high performance liquid chromatography using ODS hypersil column.

• 한국식품영양과학회지
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• 제24권5호
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• pp.695-701
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• 1995

The ethanol extract of leaf mustard(Brassica juncea) exhibiting high antimicrobial activities was fractionated in the order of hexane, chloroform, ethylacetate and butanol fractions to test antimicrobial activity. The highest antimicrobial activity for the bacteria tested was found in the ethylacetate fraction, but a lesser extent in the butanol fraction. In contrast to antimicrobial activity for the bacteria, both ethylacetate and butanol fractions showed weak antimicrobial activity for yeasts. Unknown compound A in the ethylacetate fraction which exhibited a strong antimicrobial activity was isolated by silica gel column chromatography and HPLC, and exhibited 9 times more antimicrobial activity than the ethylacetate fraction.

• Applied Biological Chemistry
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• 제30권1호
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• pp.54-59
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• 1987

미숙잠두 종자의 추출물을 silica gel chromatography, Sephadex LH-20 chromatography, TLC, 역, 순상의 HPLC 등에 의해 분획정제하여 BR 활성물질을 한국산 벼를 이용한 생물검정법에 의해 검색한 결과, 2종의 BR의 존재가 인정되었으며, BR 보다 분자량과 극성이 작은 물질의 존재가 시사되었다.

• Mass Spectrometry Letters
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• 제6권4호
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• pp.99-104
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• 2015

A liquid chromatography mass spectrometry (LC-MS) system was used to identify and distinguish oligostilbene diastereoisomers. A polyphenolic extract from Neobalanocarpus heimii known to be rich in oligostilbenes of various degrees of condensation was used as test material. Fourteen oligostilbenes were isolated from this extract on a fully automated semi-preparative HPLC system. Out of these, two pairs of dimers, one pair of trimers, two pairs of tetramers and a group of four tetramers with similar skeleton were identified as diastereoisomers. Their structures and configurations were established by spectroscopic methods. All isolated compounds were subjected to an LC-MS/MS to study their fragmentation patterns. The experiments were performed on a liquid chromatography-mass spectrometry (LC-MS) with electrospray-ionization (ESI) interface in positive mode. MS/MS spectra of each pure compound were recorded by direct infusion in identical conditions and their product ion spectra were analysed. Some subtle yet significant differences were observed between the spectra of oligostilbenes from the various diastereoisomeric series.

• 분석과학
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• 제13권3호
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• pp.399-402
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• 2000

To determine the concentrations of microcystins present in lake water or in tap water using high performance liquid chromatography, it is necessary to concentrate a large volume of water samples (about 20 L) into very small volume (0.1-0.3 mL). Concentration can be conveniently done when disc type solid phase extraction (SPE) apparatus is used. Using this apparatus we have investigated the recovery rates of three kinds of microcystins, RR, YR, LR. The recovery rates were relatively low and the reproducibilities were not good either. It is expected, however, that the appropriate selection of the disc conditioning and eluting solvents and reproducible reconcentration process after SPE will improve both the recovery rates and the reproducibilities.

• 오토저널
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• 제13권5호
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• pp.89-94
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• 1991

In the quantitative analysis of oxygenated exhaust emissions (unburned methanol, formal- dehyde) from methanol fueled vehicles, the oxygen contained in oxygenated exhaust gases lowers the FID (Flame Ionization Detector) response factor of conventional THC analyzer and leads to erroneous HC reading. For correct measurement of various HCs including oxygenated HCs emitted from FFV(Flexible Fuel Vehicle), first of all, the measurement technique of real HC emissions should be established. GC and HPLC-DNPH measuring methods specified by the EPA are used in this paper to analyze unburned methanol and formaldehyde components in the exhaust emissions. In emission test of FFV, unburned methanol and formaldehyde are emitted mostly during cold transient period, and it is shown that formaldehyde emission level is proportional to engine displacements. In view of the HC emission level, vehicle using M85 has 40% advantage over gasoline-fueled vehicle in OMHCE and has a good potential of a low emission vehicle.

• Journal of Dairy Science and Biotechnology
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• 제17권1호
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• pp.11-15
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• 1999

The objective of this study was to extract and purity the antibacterial agent from the fermented milk with Lactobacillus helveticus CH-1. The extraction and purification of antibacterial agent from the Lb. helveticus fermented milk were carried out by methanol extraction, acetone extraction, Sephadex G-200 gel filteration and thin layer chromatography and the results were as followings. The antibacterial activity of methanol-acetone extraction showed antibacterial activity against test organisms, B. subtilis, E. coli, Pseu. fluorescens, Sal. typhimurium, Shi. flexneri, and Sta. aureus. Sephadex G-200 gel chromatography showed only antibacterial activity from 33 to 37th fractions of 60 fractions. The agent purified from TLC plate confirmed the antibacterial activity by the means of bioautography.

• Journal of Applied Biological Chemistry
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• 제42권4호
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• pp.197-201
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• 1999

Antagonistic bacteria active against phytopathogenic fungi, Phytophthora capsici, Pythium ultimum, Rhizoctonia solani, Botrytis cinerea, and Fusarium oxysporum were isolated from greenhouse soils. An antifungal compound was extracted by ethyl acetate from acidified culture filtrate and purified through column chromatography and thin layer chromatography. Activity-guided bioassay was followed throughout the purification steps using Pythium ultimum as a test organism. The purified antifungal compound was identified as phenylacetic acid (PAA) based on the data obtained from IR, EI/MS, $^1H-NMR$, and $^{13}C-NMR$. Two different isolates, which had vast differences in differential characteristics except 16S rDNA sequence homology, produced the same compound, phenylacetic acid. $ED_{50}$ values of the phenylacetic acid against P. ultimum, P. capsici, R. solani, B. cinerea, and F. oxysporum were 45, 21, 318, 360, and 226 ppm, respectively.

• 한국균학회지
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• 제1권1호
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• pp.17-21
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• 1973

Fifty-eight strains of Aspergillus spp. isolated from various grains were examined for the screening of aflatoxins by the method of the Thin-Layer Chromatography (TLC), using the aflatoxin producing strain of Aspergillus flavus ATCC 15517 as a control. The results as follows: 1. Samples of Aspergillus spp. isolated from various grains were extracted with chloroform and chromatographied by the thin-layer chromatography method. 2. Three strains of Aspergillus spp. among the 58 strains can be found that the spots having the same Rf value as control with culture extract of Aspergillus flavus ATCC 15517. 3. The further prove studies of aflatoxins were proceed by the methods of in vivo and in vitro tests. And this methods considered to capable for the use of mass screening among the samples.

• 한국식품과학회지
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• 제5권2호
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• pp.78-83
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• 1973

선별된 Aspergillus 속의 한 균주인 S-1의 조(粗) naringin 분해효소의 정제에 관하여 검토한 결과 정제도의 관점에서 Sephadex G-200, starch gel electrophoresis, DEAE-Cellulose column chromatogram, 황산암모늄분획의 순위로 좋았으며 각 정제법에 따른 결과는 다음과 같다. 1) 조효소(粗酵素)를 starch gel electrophoresis 한 결과 단백 mg 당 naringinase 활성이 1,000 unit 로 정제되었다. 2. 단백 mg 당 0.37 unit, naringinase 활성인 조효소(粗酵素)를 황산암모늄분획한 결과 0.25포화 이하에서는 protein per mg 3 unit, 0.75포화 이하에서는 12 unit, 075포화 이상 완전포화 fraction 에서는 34 unit 로 정제되었으며 회수율로 볼때는 황산암모늄 0.75포화 이하에서 가장 좋았다. 3. Sephadex G-200에 의해 정제한 결과 protein per mg 1,337 unit 였으며 DEAE-Cellulose column chromatography 한 결과는 430 unit per protein mg 으로 정제되었다. 4. DEAE-Cellulose column chromatography 후 sephadex G-200에 의해 정제한 결과는 여지전기영동에 의해 단일 단백으로 나타났으며 이 단일 단백은 naringin 을 purunin 까지만 분해하였다.