• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2005년도 제36차 추계 학술발표대회
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• pp.324-328
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• 2005

Lactic acid bacteria are dominant microflora in fermented kimchi. In this study, lactic acid bacteria were isolated from 5 kinds of fermented kimchi and determined their physiologic property. Total 64 of LAB were isolated from kimch samples. In this study, 8 strains of them were selected by pH and bile salt tolerance test. HFI 58, HFI 40, and Yeulmu E strain had significant cholesterol lowering effect in vitro test. These were made of special feed of chicken by WooJin B&G. A Diet was tested for 5 weeks. The feed of special material supplement HFI58 groups had significant lower cholesterol concentration in egg yolk.

• 한국식품과학회지
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• 제32권1호
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• pp.200-205
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• 2000

췌장에서 분비되는 cholesterol esterase(CEase)와 소장의 acyl coenzyme A: cholesterol acyltransferase(ACAT)는 흡수된 콜레스테롤을 다시 ester화하는데 관여한다. 한방재료 중 건위, 진토 및 수렴지사작용 등을 가지는 초두구(Alpiniae Katusumadaii Semen)의 에탄올 추출물이 in vitro에서 cholesterol esterase활성에 대하여 강한 저해작용을 보였으며 에탄올추출물의 용매분획 중 클로로포름분획이 다른 분획들, 즉 에틸 아세테이트분획, 부탄올분획 및 물 분획보다 강하였다. 초두구의 클로로포름분획이 고 콜레스테롤식이를 섭취시킨 rat에서 혈중 콜레스테롤치를 저하시키는 효과가 있는지 측정한 실험에서 chloroform분획을 100 mg/kg 및 150 mg/kg 섭취시켰을 경우 각각 총cholesterol 함량이 11.8% 및 20.8% 감소하였으며 HDL-cholesterol 함량 및 동맥경화지수는 정상군에 가깝게 회복된 것으로 나타났다. 또한 초두구 chloroform분획의 독성검사 결과 mouse에서 $LD_{50}$은 1,625mg/kg으로 산출되었다.

• 한국식품과학회지
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• 제38권5호
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• pp.674-678
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• 2006

발아현미의 섭취에 의한 흰쥐의 비만억제 및 콜레스테롤 저하 효과를 조사하기 위하여 대조군, 백미군, 현미군, 발아현미군의 4가지 처리군으로 나누어 흰쥐에 고지방, 고콜레스테롤 식이를 공급하면서 5주간 사육후 효과를 비교하였다. 체중의 경우 모든 처리군에서 유의적인 차이를 나타내지 않았으나 발아현미군에서 5주 경과후의 체중에서 $471.2{\pm}17.8\;g$으로 가장 작은 값을 나타내었다. 체중증가량과 식이효율의 경우에도 발아현미군이 각각 $310.6{\pm}14.7\;g$과 $41.8{\pm}2.0%$로 유의적인 수준은 아니지만 가장 낮은 값을 나타내었다. 혈중 중성지질 및 콜레스테롤의 경우 발아 현미군은 5주 경과시 각각 $54.0{\pm}23.4\;mg/dL$, $64.8{\pm}14.7\;mg/dL$로 백미군, 현미군에 비해 크게 낮을 뿐만 아니라 부고환지방, 신장 지방 중량에서도 낮은 값을 나타내었다. 간의 총 지질과 총 콜레스테롤에서 발아현미군은 $216.3{\pm}35.7\;mg/g$ liver 와 $16.5{\pm}0.7\;mg/g$ liver로 기타 처리군에 비해 낮은 값을 나타내었으며, 변의 총 지질, 총 콜레스테롤, 중성지질에서도 각각 $165.0{\pm}22.2\;mg/g$ feces, $26.5{\pm}2.7\;mg/g$ feces, 3.4 mg/g feces로 기타 처리군에 비해 유의적으로 높은 값을 나타내었다.

• 한국식품영양과학회지
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• 제30권1호
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• pp.107-111
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• 2001

This study was carried out to investigate the effect of Meristotheca papulosa on lipid concentration of serum and liver in rats fed high fat diet for 4 weeks. Experimental groups ere divided into normal group (5% corn oil), high fat diet group (5% corn oil+20% lard), high fat diet and 5% or 10% Meristotheca papulosa treated group. The weights of kidney, heart and tests were not significantly different among the group, but weight of liver was significantly decreased by supplement of Meristotheca papulosa (10%). The contents of serum total lipid, trigly-ceride, total cholesterol, free cholesterol, cholesterol ester and atherogenic index were decreased, while HDL-cholesterol was increased by Meristotheca papulosa supplementation compared to high fat diet group. Meristotheca papulosa supplement has obvious effects on lowering hepatic total lipid, triglyceride and total cholesterol. Our findings suggest that Meristotheca papulosa supplement can improve lipid metabolism in high fat fed rats.

• 생명과학회지
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• 제12권1호
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• pp.106-112
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• 2002

췌장에서 분비되는 콜레스테롤 에스터레이즈(pCEase)와 소장의 acyl coenzyme A:cholesterol acyltransferase (ACAT)는 흡수된 콜레스테롤을 다시 ester화하는데 관여한다. 한방재료 중 순환촉진작용 및 건위작용 등을 가지는 계피(Cinnamomi cortex)의 에탄올 추출물이 in vitro에서 pCEase 활성에 대하여 강한 저해작용을 보였으며 에탄올 추출물의 용매분획 중 클로로포름분획이 다른 분획들, 즉 에틸 아세테이트분획, 부탄올분획 및 물 분획보다 저해활성이 강하였다. 계피의 클로로포름분획이 고콜레스테롤식 이를 섭취시킨 rat에서 혈중 콜레스테롤치를 저하시키는 효과가 있는지 측정한 실험에서 클로로포름분획을 150 mg/kg 섭취시켰을 경우 고콜레스테롤식이만은 투여한 대조군에 비하여 총cholesterol 함량이 25.1% 감소하였으며, HDL-cholesterol 함량도 33.4$\pm$1.82 g/dl로 정상군 수준으로 회복되었고 동맥경화지수 역시 현저하게 감소된 것으로 나타났다. 콜레스테롤 생합성에 관여하는 효소인 HMG-CoA reductase 활성을 측정한 결과, 고콜레스테롤 식이에 의해 감소되었던 활성이 계피 클로로포름분획의 투여량을 증가시킬수록 증가하였으나 150 mg/kg 투여군 에서도 정상군에 비하여 유의적인 감소를 보였다. 또한 계피 클로로포름분획의 독성검사 결과, mouse에서 LD$_{50}$ 은 1,300 mg/kg으로 산출되었다.

• 한국식품영양과학회지
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• 제28권6호
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• pp.1412-1423
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• 1999

This review contains a discussion of the physiological activity of the components of Allium vegetables. Organosulfur compounds in Allium vegetables, such as ajoene, diallyl sulfides and S allylcysteine, have cancer preventive activity in chemically induced animal cancer models. They also have inhibitory effects on proliferation of cancer cells in vitro. Allium vegetables have lipid and cholesterol lowering effect, and platelet aggregation inhibitory activity that help the prevention of cardiovascular diseases. Sulfur con taining compounds, especially allicin and ajoene, have antimicrobial activities against gram negative, positive bacteria and fungi. Moreover, Allium organosulfur compounds such as S allylcysteine showed reducing effects on the senescence related symptoms including cognition. Allium organosulfur compounds have significant importance in food industry as both biologically active ingredients and savory.

• 한국식품영양과학회지
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• 제27권6호
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• pp.1211-1216
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• 1998

The study was conducted to investigate the effect of cholesterol and fiber on serum lipid metabolism in rats. Male Sprague Dawley rats were administrated 1% cholesterol and 0.25% sodium cholate to induce hypercholesterolemia and were fed on diet containing three concentrations(0%, 5%, 10%) of cellulose and pectin, respectively. The rats were sacrified after 5 weeks of feeding periods. Serum triglyceride concentration was significantly decreased in proportion to level of fiber. Phospholipid concentration was more decreased in 10% fiber groups than 5% fiber groups but did not show any difference by fiber types. Free cholesterol and cholesteryl ester concentrations in serum were significantly decreased by fiber supplement and pectin was more effective than cellulose in cholesterol lowering effect. Whereas HDL cholesterol concentration and HDL C/TC were increased in proportion to level of fiber and the effect of pectin was not more significant than that of cellulose. Serum chylomicron cholesterol concentration also showed significant decrease by pectin supplement and LDL , VLDL cholesterol concentrations were more significantly decreased in 10% fiber groups than those of 5% fiber groups. The results indicate that 10% pectin may have beneficial roles in hypercholesterolemia.

• Journal of Nutrition and Health
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• 제20권1호
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• pp.10-14
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• 1987

The effect of the addition of the white powder separated from Sogi (Gyrophora esculenta) by the extraction methods with ethanol and acetone was checked by the feeding experiment in rats fed with a high cholesterol diet. From this experiment, it was confirmed that this material, which was assumed to be the mixture of gyrophoric acid and the decomposition products, could lower both liver and plasma cholesterol levels.

• 한방재활의학과학회지
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• 제21권1호
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• pp.23-33
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• 2011

Objectives : The present study investigated effects of Artemisia Capillaris Thunberg ethanol extract(EtOH ext). on lowering lipid, anti-oxidation and concentration of plasma inflammatory mediators using rat fed on high oxidized fat. Methods : We divided fat sprague-dawley rats fed on high oxidized into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 4 weeks. We measured concentration of plasma free fatty acid(FFA), plasma triglyceride, plasma total cholesterol, and plasma low density lipoprotein-cholesterol(LDL-cholesterol), plasma high density lipoprotein-cholesterol(HDL-cholesterol), concentration of liver total cholesterol and liver triglyceride (TG), concentration of plasma thiobarbituric acid reactive substance(TBARS) and liver thiobarbituric acid reactive substance(TBARS), glutathione peroxidase (GSH-Px) activity, superoxide dismutase(SOD) activity and catalase(CAT) activity, plasma nitric oxide(NO), ceruloplasmin and ${\alpha}-glycoprotein$. Results : 1. The Artemisia Capillaris Thunberg EtOH ext. groups showed low concentration of plasma FFA, plasma triglyceride, plasma total cholesterol and plasma LDL-cholesterol compared to control group. However, concentration of plasma HDL-cholesterol was increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 2. Concentration of liver total cholesterol and liver TG showed a significantly decrement in all Artemisia Capillaris Thunberg EtOH ext. groups than that of control group. 3. The Artemisia Capillaris Thunberg EtOH ext. groups showed lower values in concentration of plasma TBARS and liver TBARS than that of control group. The values of GSH-Px activity, SOD activity and CAT activity were increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 4. The values of plasma NO, ceruloplasmin and ${\alpha}-glycoprotein$ were decreased in Artemisia Capillaris Thunberg EtOH ext. groups. Conclusions : Based on the results in this study, the Artemisia Capillaris Thunberg EtOH ext. showed a positive effect in lowering lipid, anti-oxidation and decrement of plasma inflammatory mediators.

• Preventive Nutrition and Food Science
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• 제8권1호
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• pp.13-18
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• 2003

In experiment 1, rats (n＝6) fed diet containing 10 g/kg cholesterol for 4 wk (control) with either no amaranth (control), amaranth grain (300 g/kg, AG) or amaranth oil (90 g/kg, AO). Both the AG and AO groups had lower concentration of serum and hepatic cholesterol and triglyceride than the controls (p < 0.05). Fecal excretions of cholesterol and bile acid in AO group increased about 4 fold and 2 fold, respectively, while AG affected only bile acid excretion (p < 0.05). In experiment 2, rats (n＝6) were fed the cholesterol diet for 4 wk and injected intraperitoneally with saline (control) or amaranth squalene (AS) for 7d. The hypolipidemic effect of AS was evident in both serum and liver. Fecal excretions of cholesterol and bile acid were greater (p < 0.05) in AS than control. HMG-CoA (3-hydroxy-3-methylglutaryl coenzyme A) reductase activity was reduced in AS group (11.6%, p=0.13). This study suggests that the cholesterol-lowering effect of AS is mediated by greater fecal elimination of steroids through interference with cholesterol absorption.