• Title/Summary/Keyword: chlorophyll degradation

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Effect of Limiting Factors for Hydrogen Production in Sulfur Deprived Chlamydomonas Reinhardtii (황결핍 된 Chlamydomonas Reinhardtii 배양액에서 수소생산을 위한 제한 인자들의 영향)

  • Kim, Jun-Pyo;Sim, Sang-Jun
    • Transactions of the Korean hydrogen and new energy society
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    • v.17 no.3
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    • pp.286-292
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    • 2006
  • Chlamydomonas reinhardtii is a green algae that can use light energy and water to produce hydrogen under anaerobic condition. This work reports the effect of limiting factors on hydrogen production in sulfur deprived anaerobic C. reinhardtii culture. In order to confirm the relationship between hydrogen production and limiting factors such as residual PSII activity and endogenic substrate degradation, the increase in chlorophyll concentration and the decrease in starch concentration was investigated during sulfur deprivation. The overall hydrogen production increased depending on cell density in range of $0.4{\sim}0.96\;g$ DCW/l. At this time, the increase in chlorophyll concentration during 24 h after sulfur deprivation increased in proportion to hydrogen production, however, the decrease in starch concentration was not proportional to that. Therefore, hydrogen production under sulfur deprivation using green alga was closely associated with the residual PSII activity than the endogenic substrate degradation.

Phytoremediation potential of indigenous Ghanaian grass and grass-like species grown on used motor oil contaminated soils

  • Akutam, Abednego;Pappoe, Alexander Nii Moi;Armah, Frederick Ato;Enu-Kwesi, Lewis
    • Journal of Ecology and Environment
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    • v.37 no.2
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    • pp.41-51
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    • 2014
  • This study investigated the ability of Bothriochloa bladhii (Retz.) S.T. Blake (Poaceae), Cyperus ligularis L. (Cyperaceae), Commelina erecta L. (Commelinaceae), Mariscus umbellatus (Rottb.) Vahl (Cyperaceae), Fimbistylis miliacea L. (Cyperaceae) and Torulinium odoratum L. (Cyperaceae) to clean up various levels of used motor oil (UMO) contaminated soils. The plants were grown in 2 kg garden soils treated to 0%, 1%, 5% and 10% levels of UMO contamination. The plant growth parameters, chlorophyll contents and dry weight of test plants were measured. The phytoremediation ability of these test plants were assessed by measuring the uptake of hydrocarbons in terms of total hydrocarbon content (THC) as well as their percentage degradation values. There was significant (P < 0.05) reduction in leaf chlorophyll contents and dry weights of the test plant species planted in UMO contaminated soils. THC as well as the percentage uptake (or degradation) of hydrocarbons were both lowest in C. ligularis but highest in T. odoratum in all cases. The phytoremediation potential of test plants was highest in soils contaminated with 5% UMO. Based on the results of this study, all test plants with the exception of C. ligularis were potentially capable of undertaking phytoremediation. However, B. bladhii and T. odoratum proved most effective in the uptake and degradation of UMO.

Changes in Chlorophylls and Carotenoids of Mustard Leaf Kimchi during Fermentation and Their Antioxidative Activities on the Lipid Oxidation (갓김치의 발효과정 중 Chlorophylls 및 Carotenoids의 변화와 동획분의 항산화성)

  • 송은승;전영수;최홍식
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.4
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    • pp.563-568
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    • 1997
  • Changes of chlorophylls and carotenoids and antioxidative characteristics of crude chlorophylls and carotenoids(CCC) of mustard leaf kimchi(MLK) were investigated during the fermentation at 15$^{\circ}C$ for 25 days. At the beginning of fermentation chlorophylls were decreased rapidly, however, all chlorophylls and chlorophyllides were converted to pheophytins and pheophorbides at the final stage of fermentation. Total chlorophyll content remained constantly during the fermentation but 43.7% destruction of carotenoids was observed at 25 days of fermentation. Antioxidative activities of CCC from MLK against autoxidation of linoleic acid were significant(p<0.05) and much higher than those of $\alpha$-tocopherol. Fermentation period did not affect antioxidative activities of CCC of MLK. When the CCC of MLK was added to the autoxidation system of linoleic acid, degradation of total chlorophylls were observed to be slower than that of total carotenoids.

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An Effective Defensive Response in Thai Aromatic Rice Varieties(Oryza sativa L. spp. indica) to Salinity

  • Cha-um, Suriyan;Vejchasarn, Phanchita;Kirdmanee, Chalermpol
    • Journal of Crop Science and Biotechnology
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    • v.10 no.4
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    • pp.257-264
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    • 2007
  • Rice is one of the world's staple crops and is a major source of carbohydrate. Rice is exported from several countries, providing a major source of income. There are many documents reporting that rice is a salt-sensitive crop in its developmental stages. The objective of this investigation is to evaluate the effective salt-tolerance defense mechanisms in aromatic rice varieties. Pathumthani 1(PT1), Jasmine(KDML105), and Homjan(HJ) aromatic rice varieties were chosen as plant materials. Rice seedlings photoautotrophically grown in-vitro were treated with 0, 85, 171, 256, 342, and 427 mM NaCl in the media. Data, including sodium ion$(Na^+)$ and potassium ion$(K^+)$ accumulation, osmolarity, chlorophyll pigment concentration, and the fresh and dry weights of seedlings were collected after salt-treatment for 5 days. $Na^+$ in salt-stressed seedlings gradually accumulated, while $K^+$ decreased, especially in the 342-427 mM NaCl salt treatments. The $Na^+$ accumulation in both salt-stressed root and leaf tissues was positively related to osmolarity, leading to chlorophyll degradation. In the case of the different rice varieties, the results showed that the HJ variety was identified as being salt-tolerant, maintaining root and shoot osmolarities as well as pigment stabilization when exposed to salt stress or $Na^+$ enrichment in the cells. On the other hand, PT1 and KDML105 varieties were classified as salt-sensitive, determined by chlorophyll degradation using Hierarchical cluster analysis. In conclusion, the HJ-salt tolerant variety should be further utilized as a parental line or genetic resource in breeding programs because of the osmoregulation defensive response to salt-stress.

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Glyphosate Toxicity: I. Long Term Analysis of Shikimic Acid Accumulation and Chlorophyll Degradation in Tomato Plant (Glyphosate 독성(毒性): I. Glyphosate 처리(處理)가 토마토의 Shikimic Acid의 축적(蓄積)과 엽록소(葉綠素)의 분해(分解)에 미치는 영향(影響))

  • Kim, Tae-Wan;Amrhein, Nikolaus
    • Korean Journal of Weed Science
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    • v.15 no.2
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    • pp.141-147
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    • 1995
  • Glyphosate(N-[phosphonomethyl]glycine) applied to the assimilate-exporting leaves or sprayed to the whole plants of tomato(Lycopersicon esculentum Mil var. Moneymaker). Glyphosate induced the rapid accumulation of shikimic acid within 24 h. The accumulation of shikimic acid companied with chlorophyll loss in meristematic leaves, i.e. apical leaves. The chlorosis was acropetal in apical region of young growing leaf. The degradation of chlorophyll seems to be a secondary or tertiary effect of glyphosate. However, the level of shikimic acid accumulated was reduced except for roots and apical leaves from 5 days after treatment. The accumulating levels are considerably differed through the applicated regions. The level of shikimic acid is highest at the apical meristem 4 days after the application to 3rd old leaf.

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HPLC Analysis of Biomass and Community Composition of Microphytobenthos in the Saemankeum Tidal flat, West Coast of Korea (한국 서해 새만금 갯벌에서 저서미세조류의 생체량과 군집조성에 대한 HPLC 분석)

  • OH Seung-Jin;MOON Chang-Ho;PARK Mi-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.3
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    • pp.215-225
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    • 2004
  • Biomass and community composition of microphytobenthos in the Saemankeum tidal flat were studied by HPLC analysis of the photosynthetic pigments from November 2001 to November 2002. The environmental factors of sediment were also investigated to examine the relationship between microphytobenthos biomass and sedimentary environments. The detected photosynthetic pigments of microphytobenthos were chlorophyll a, b, c, fucoxanthin, 19'-hexanoyloxyfucoxanthin, violaxanthin, diadinoxanthin, alloxanthin, diatoxanthin, zeaxanthin+lutein, peridinin and beta-carotene. Pheophytin a, the degradation product of chlorophyll a, was also detected. The results of pigmen analysis suggest the presence of diatom (fucoxanthin), euglenophytes (chlorophyll b), chlorophytes (chlorophyll b + lutein), cyanobacteria (zeaxanthin), cryptophytes (alloxanthin), chrysophytes (fucoxanthin + violaxanthin), prymnesiophytes (19'-hexanoyloxyfucoxanthin) and dinoflagellates (peridinin). Chlorophyll a concentration in the top 0.5 cm of sediment was in the range of $0.24\;mg{\cdot}m\^{-2}\;-32.11\;mg{\cdot}m\^{-2}$ in the study area. The increase of chlorophyll a concentration in the spring indicates the occurrence of a microphytobenthic bloom. In the summer, there was a sharp decrease of the chlorophyll a concentration which was probably due to high grazing activity by macrobenthos. The annual mean chlorophyll a concentration in the study area was low compared to that in most of other tidal flat areas probably due to active resuspension of microphytobenthos and high grazing activity by macrobenthos. There was no clear relationship between microphytobenthos biomass and sedimentary environments because of a large variety of physical, chemical and biological factors, Pigment analysis indicated that while diatoms were dominated in the microphytobenthic community of the Geojon tidal flat, euglenophytes and/or chlorophytes coexisted with diatoms in the Mangyung River tidal flat.

Effect of Storage Conditions on Quality Stability of Dried Laver(Porphyra tenera) (건조김의 품질 안정성에 미치는 저장 조건의 영향)

  • 조길석
    • Food Science and Preservation
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    • v.10 no.1
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    • pp.32-36
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    • 2003
  • Quality stability of the dried layer Porphyra tenera depending on various light sources, water activities, packaging materials and storage temperatures were investigated by peroxide value and chlorophyll degradation. Major fatty acids of dried layer were 45.7% eicosapentaenoic acid and 13.6% palmitic acid. Quality stability was increased in order of darkness, incandescent and fluorescent increased sharply with the decrease of water activity and temperature, and also improved by the packaging material with strong barriers of water vapor, oxygen and light.

Assessment of environmental impacts of LID technologies on vegetation

  • Choi, Hyeseon;Hong, Jungsun;Geronimo, F.K.F.;Kim, Lee-Hyung
    • Membrane and Water Treatment
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    • v.10 no.1
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    • pp.39-44
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    • 2019
  • LID facilities do not consider environmental factors, and due to inappropriate vegetation planting causing degradation in efficiency due to plant damage and difficulty in maintenance. Therefore, in this study, assessment of impact environmental factor by seasonal variation of chlorophyll and growth of vegetation planted in LID technologies and change of pollutant reduction were conducted. In the case of B-SJ and B-RI, growth rate decreased after summer (August), and B-MG showed steady growth until autumn (September). Chlorophyll was found to increase during spring season while it decreased during autumn season. The chlorophyll concentration was found to affect the plant growth pattern. TN reduction efficiency was highest with greater than 80% efficiency in summer, and it was analyzed that plants were identified as the main factor affecting the seasonal reduction efficiency of TN. Also, temperature and relative humidity were analyzed to affect plant growth, activity and pollutant removal efficiency. Plant type and growth pattern are considered as factors to be considered in selection of appropriate plant types in LID technologies.

Role of Plant Hormones in the Senescing Detached Leaves of Phaseolus vulgaris (강남콩 잎의 노화에 있어서 식물 호르몬의 역할)

  • 이동희;서영희;김영상
    • Journal of Life Science
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    • v.7 no.4
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    • pp.297-308
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    • 1997
  • Role of plant hormones on the leaf senescence of Phaseolus vulgaris were investigated by measuring the disassembly of chlorophyll-protein complexes in detached leaves treated with NAA, GA$_{3}$ , or BA. The loss of chlorophyll that was characteristic of leaf senescence induced disassembly of chlorophyll-protein complexed. During dark-induced senescence, PSI complex was rapidly degraded after the early stage, whereas RC-Core3 was slightly increased until the middle stage and slowly decreased thereafter. And gradual degradation of trimeric LCHII progressed after the late stage of senescence. Exogenous application of NAA and GA$_{3}$ had little or no effect in protecting disassembly of chlorophyll-protein complexes during leaf senescence compared to control. However exogenous BA application strongly leaves. In the simultaneous treatment of plant hormones and light, BA application under illumination of light was most effective in the stability of chlorophyll-protein complexes, particularly PSI, LHCII, RC-Core2, RC-Core3 and SC-1. these results suggest, therefore, that simultaneous application of BA and light induced synergistic effect on the stability off chlorophyll-protein complexes during leaf senescence.

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Influence of Maturity on the Plastid Pigments and Chromatic Characteristics of Paddy Tobacco (연초 수확엽의 숙도가 색채 및 색소물질 함량에 미치는 영향)

  • Lee, Chul-Hwan;Jin, Jeong-Eui;Han, Chul-Soo
    • Journal of the Korean Society of Tobacco Science
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    • v.18 no.1
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    • pp.5-11
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    • 1996
  • Field experiment was conducted to get the basic information about the difference in color and plastid pigments at the different stage of harvest of flue-cured tobacco cultivated in paddy field, and compared to upland ones. All tested crops were primed in lots of 3 to 4 at each priming, beginning at the bottom of the plant. Leaf pigments and chromatic values of tobacco leaves were determined in samples collected at weekly intervals, and according to analyses obtained from 4 stalk positions. By the degree of maturity, leaves harvested were separated with visual characters into four classes of immature, mature, ripe and mellow. regardless of stalk position, chlorophyll contents was higher in the order of immature > mature > ripe > mellow, respectively. In fresh leaves, chlorophyll levels was higher with ascending stalk position. chlorophyll a and b in cured leaves were less than 10% of the amount present at harvest, and it was estimated that maturity condition also influenced the rates of degradation of plastid pigments. Fully mature leaves of upland diverted from paddy field showed slightly higher in chlorophyll contents than in those of upland tobacco ones but somewhat lowered in $L^*$ and $b^*$ values, and there was no difference in $a^*$ value.

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