• 생약학회지
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• 제25권2호
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• pp.140-143
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• 1994

During the serial attempts to identify the chemical and biological characteristics of Ailanthi Cortex Radicis, the root bark of Ailanthus altissima (Simaroubaceae), we find out the serious toxic effect on kidney by chloroform fraction of the methanolic extract of the herb drug. The toxicities were revealed as the increase of urea nitrogen amount in blood and lactate dehydrogenase and ${\gamma}-glutamyltransferase$ activities in urine and the decrease of the concentration of glutathione and both of protein bound and non-protein bound -SH in kidney tissue.

• 한국식품조리과학회지
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• 제14권5호
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• pp.577-583
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• 1998

Methanol extracts of Commelina communis, a Korean wild edible plant showing hypoglycemic effect on diebetic rats were fractionated systematically by hexane, chloroform, butanol, and water, and these four fractions were administered orally to streptozotocin-induced diabetic rats to evaluate the hypoglycemic effect. The butanol and water fractions of Commelina communis lowered blood glucose levels of diebetic rats at a dose of 500 mg/kg/day. Administration of each fraction except that of chloroform increased the plasma protein levels. Liver protein levels were significantly higher in the butanol- and water fraction-fed groups. The results suggest that the butanol fraction of Commelina communis has hypoglycemic effect on diabetic rats and the intake of Commelina communis may be beneficial for the management of diabetes mellitus.

• Natural Product Sciences
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• 제9권1호
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• pp.1-3
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• 2003

Hemidesmus indicus var. indicus belonging to the family Asclepiadaceae was screened for its activity against Helicobacter pylori(Hp) human isolates. Flowering and vegetative period samples were analysed. Aqueous (hot and cold) and solvent extracts (acetone, chloroform and methanol) were screened. Among them chloroform extract was observed to recover bioactive principles with low MIC and MLC. MIC was $75\;{\mu}g$ in both seasons and MLC was $100\;{\mu}g$ for vegetative and $75\;{\mu}g$ for flowering periods respectively. Extracts from samples collected during flowering period was better than that of vegetative period.

• Archives of Pharmacal Research
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• 제26권10호
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• pp.816-820
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• 2003

The chloroform extract of the aerial part of Limnophila geoffrayi showed antimycobacterial and antioxidant activities. Bioassay-guided fractionation has led to the isolation of the flavones nevadensin (5,7-dihydroxy-6,8,4'-trimethoxyflavone, 1) and isothymusin (6,7-dimethoxy-5,8,4'-trihydroxyflavone, 2). Both compounds 1 and 2 exhibited inhibition activity against Mycobacterium tuberculosis, with equal MIC value of $200{\;}\mu\textrm{g}/mL$. Only compound 2 exhibited antioxidant activity against the radical scavenging ability of DPPH, with the $IC_{50}$ value of $7.7{\;}\mu\textrm{g}/mL$. The crude hexane, chloroform and methanol extracts as well as the pure compounds 1 and 2 did not exhibit mutagenic activity in the Bacillus subtilis recassay.

• 약학회지
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• 제43권6호
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• pp.771-775
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• 1999

The antimutagenic activities of the total extract and several fractions of starfish, Asterina pectinifera (Asteriidae), were investigated in vitro by SOS chromotest with Escherichia coli PQ37 and Ames test with Salmonella typhimurium TA100. When various fractions was tested, the chloroform and butanol fractions showed low induction factors, which means both fractions increased antigenotoxicity against the base substitution mutagen MNNG. Even though higher antigenotoxic effect of the chloroform fraction, no effective result of Ames test was found in revertant formation of S. typhimurium TA100. The most effective antigenotoxic and antimutagenic fraction was a butanol one: i.e., When 0.5 mg/tube of butanol fraction was applied, the induction factor was 0.68. As the concentration of the fraction was increased the formation of revertants of S. typhimurium TA100 by about 81%. There was no cytotoxic effect of butanol fraction against S.typhimurium TA100. This result might be useful for further study to search a possible anticancer agent from the starfish, Asterina pectinifera.

• 한국식품영양과학회지
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• 제28권3호
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• pp.593-598
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• 1999

This study was designed to investigate the possible utilization of Zizyphus jujuba leaves as a source of functional ingredients. The physiological activity of different solvent fractions prepared from ethanol extract of Zizyphus jujuba leaves were analyzed. Xanthine oxidase inhibitory effect was very high in all fractions except chloroform fraction. The very high electron donating ability was observed in the ethylacetate fraction and the effect was similar to 0.1% tocopherol. Nitrite scavenging effect of all fractions was more than 40% even at low concentration of 1mg/ml and was increased with increasing concentration. Angiotensin I converting enzyme inhibitory activity was appeared in ethyl acetate and chloroform fractions only at high concentraton.

• 한국식품영양과학회지
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• 제39권7호
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• pp.933-940
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• 2010

흑마늘의 기능성을 분석하기 위하여 용매별 계통 분획물을 만들어 100, 250, 500 및 $1,000\;{\mu}g/mL$ 농도에서 항산화 활성을 측정하였다. 총 페놀 및 플라보노이드 화합물의 함량은 chloroform, ethyl acetate 및 hexane 분획물에서 유의적으로 높아 butanol, methanol 및 물 분획물에 비하여 5.5~11.6배 더 많았다. 흑마늘 계통 분획물의 항산화 활성은 시료의 첨가농도가 증가함에 따라 그 활성도 증가하였으며, chloroform과 ethyl acetate 분획물의 활성이 유의적으로 높았다. DPPH 라디칼 소거능은 $1,000\;{\mu}g/mL$ 농도에서는 butanol과 물 분획물을 제외한 모든 시료 모두에서 50% 이상의 활성을 나타내었다. ABTS 라디칼 소거능의 경우 hexane, chloroform, ethyl acetate 분획물에서 비교적 활성이 높아, $1,000\;{\mu}g/mL$ 농도에서는 70% 이상의 소거능을 보였다. $1,000\;{\mu}g/mL$ 농도에서 hydroxyl radical 소거능은 50.27~81.02%의 범위로 높은 반면 SOD 유사활성은 26.73~47.64%로 비교적 그 활성이 낮았다. Linoleic acid 반응계에서 항산화 활성을 측정한 결과 저장기간이 길수록, 시료의 첨가 농도가 높을수록 항산화 활성이 더 높았으며, 또 극성 용매보다는 비극성용매 분획물의 활성이 유의적으로 높았다. 아질산염 소거능의 경우 butanol 분획물을 제외한 모든 시료에서 비교적 그 활성이 높아 $100\;{\mu}g/mL$ 농도에서도 50% 이상의 소거능을 보였다.

• 한국식생활문화학회지
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• 제19권2호
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• pp.170-176
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• 2004

천연첨가물검색의 일환으로 초피의 용매 추출물의 항산화성과 항균성을 실험하고, 소비자의 수용도를 검토하기 위하여 초피를 첨가한 육원전의 관능 검사를 실시한 결과는 다음과 같았다. 1. 초피 용매 추출물들의 옥수수유에 대한 항산화 효과는 가장 많이 사용되는 합성항산화 제인 BHT과 같은 농도에서 BUex는 월등히 높았으며, MEex, EAex는 유사한 경향을 나타냈다. 2. 초피의 용매 추출물의 중 CHex와 BUex가 Escheria coli, Salmonella typhimurium, Psudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus 및 Listeria monocytogenes 모두에 대하여 항균효과를 보였다. 3. 초피 가루를 식품에 첨가하여 제조한 육완자전에 대한 소비자의 기호도를 검사한 결과는 0.1%를 첨가한 경우는 초피를 첨가하지 않은 본래의 육완자전과 같은 수준의 기호도를 나타내므로써(P<0.05) 초피 가루에 의한 이미(異味)가 없어 육와자전 제조에 초피가루를 사용 할 수 있을 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권10호
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• pp.4631-4635
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• 2016

Background: The plant Ecballium elaterium (L.) A. Rich, belongs to the Cucurbitaceae family which occupies an important position in traditional medicine prescriptions. It has been reported that a freeze-dried aqueous extract of E. elaterium fruits has cytotoxic effects on the AGS human stomach adenocarcinoma cell line. We here focused on anticancer effects of the main chemicals purified from E. elaterium fruits. Materials and Methods: We isolated cucurbitacins D, E, and I from chloroform, and ethyl acetate fractions of a methanolic extract of E. elaterium fruits and assessed their cytotoxic effects on the AGS cell line by MTT assay. The methanolic extract was fractionated to petroleum ether, chloroform, and ethyl acetate fractions. The compounds isolated by column chromatography were identified by NMR spectroscopy. Results: After 24 h of incubation with AGS cells, the IC50 values were 0.3, 0.1, and $0.5{\mu}g/ml$ for cucurbitacins D, E, and I respectively. Conclusions: This finding suggests that because of its cucurbitacins, E. elaterium fruit may have some cytotoxic effects on gastric cancer cells. Also, compared with D and I, cucurbitacin E showed greater potency in this regard.

• 한국식품영양과학회지
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• 제35권2호
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• pp.182-186
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• 2006

본 연구에서는 연잎 추출분획물의 항산화 활성을 비교, 검토하고자 하였다. $70\%$ ethanol로 추출한 추출수율은 $8.16\%$였으며 이를 hexane, chloroform, ethyl acetate, butanol, water로 순차 분획한 수율은 butanol층이 $36.12\%$로 가장 높은 수율을 보였으나 대체적으로 극성이 높을수록 높은 분획수율을 나타내었다. 분회물간 총 페놀함량 측정결과 ethyl acetate 분획물의 총 페놀함량이 가장 높게 나왔으며 전자공여능 측정결과 또한 ethyl acetate 분획물에서 가장 우수한 결과를 나타내었다. Ethyl acetate 분획물의 전자공여능은 BHA, BHT와 비교한 결과 BHA와 유사한 결과를 나타내었으며 BHT보다 우수한 값을 나타내었다. 항산화 활성이 높게 나타난 ethyl acetate 분획물을 취하여 TBA가에 의한 지질과산화를 측정한 결과 BHA가 가장 우수한 활성을 보여 주었으며 ethyl acetate 분획물은 BHT보다도 활성이 떨어졌으나 이는 ethyl acetate 분획물이 단일물질로 분리정제가 이루어지지 않은 것으로 malonaldehyde 이외 다른 성분이 BHA와 반응하여 발색한 것으로 추측되어 졌다. AV와 POV 측정 결과 BHA가 가장 우수한 활성을 보여주었으나 ethyl acetate 분획물과 유의적인 차이는 없었으며 BHT에 비해서는 우수한 활성을 나타내었다.