• Journal of Microbiology and Biotechnology
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• v.18 no.6
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• pp.1076-1080
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• 2008

Glucagon, a peptide hormone produced by alpha-cells of Langerhans islets, is a physiological antagonist of insulin and stimulator of its secretion. In order to improve its bioactivity, we modified its structure at the C-terminus by amidation catalyzed by a recombinant amidase in bacterial cells. The human gene coding for glucagon-gly was PCR amplified using three overlapping primers and cloned together with a rat ${\alpha}$-amidase gene in plasmid pMGA. Both genes were expressed under control of the strong constitutive promoter of aph and secretion signal melC1 in Streptomyces lividans. With Phenyl-Sepharose 6 FF, Q-Sepharose FF, SP-Sepharose FF chromatographies and HPLC, the peptide was purified to about 93.4% purity. The molecular mass of the peptide is 3.494 kDa as analyzed by MALDI TOF, which agrees with the theoretical mass value of the C-terminal amidated glucagon. The N-terminal sequence of the peptide was also determined, confirming its identity with human glucagon at the N-terminal part. ELISA showed that the purified peptide amide is bioactive in reacting with glucagon antibodies.

• Journal of Haehwa Medicine
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• v.12 no.2
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• pp.11-40
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• 2004

This study analyzed the contents of the research papers concerning the vascular dementia(VD) recorded in Chinese medical journal published over the period between 1998 and 2000. As a result, the following conclusion was drawn. The Chinese medical category of VD includes Sun-Mang(善忘), Chi-Mae, Jung-Pung-Chi-Mae and so on, and the Major mechanism of the VD is Bon-Heo- Pyo-Sil(state of deficient vital essence and excessive pestilential vapor;本虛標實), Bon(本) includes Sin-Jeong-Heo-Son(腎精虧損), Ki-Hyeol-Hyu-Heo(氣血虧虛) and Pyo(標) includes Dam-Chae-Eo-Jo(痰滯瘀阻), Ki-Chae-Hyeol-Eo(氣滯血瘀), Gan-Yang-Sang-Hang(肝陽上亢). It is mainly used DSM and evidence of brain-vascular disease in brain CT or MRI as the criterion for diagnosis in Western medicine and MMSE, HDS, ADL is used as assistant diagnostic indicator. "Principle of clinical study guidance for treatment of dementia by Chinese medicine, Western medicine"("中藥新藥治療痴呆 病的臨床硏究指導原則") is mainly used as the criterion for diagnosis in Chinese medicine. It is mainly used "Principle of clinical study guidance for treatment of senile disease by Chinese medicine, Western medicine"("中藥新藥治療老年病臨床硏究指導原則"), "Principle of clinical study guidance for treatment of dementia by Chinese medicine, Western medicine" as the criterion for treatment effect evaluation, and score change of MMSE and HDS is used as assistant evaluation indicator. The research papers reported that the use of prescriptions such as Hwan-Chong-Dan(還聰丹), Noi-Hyeol-So-Tong-Gu-Bok-Yaek(腦血疏通口服液), Yik-Gi-Gyo-Nang(益智膠囊), Cheon-Ma-Chok-Gi-Chung-Je(天麻促智沖劑), Sam-Gap-San-Ga-Mi-Bang(三甲散加味方), Seon-Ryong-Gyo-Nang(仙龍膠囊), Seong-Noi-Tang II(醒腦湯II號), Bo-Sin-Geon-Noi-Tang(補腎健腦湯), Ga-Mi-Tong-Gyu-Hyal-Hyeol-Tang(加味通竅活血湯), So-Jo-Yik-Ji-Bang(疏調益智方), Tong-Gyu-Hwal-Hyeol-Tang-Ga-Gam(通竅活血湯加減), Yik-Sin-Seong-Noi-Tang(益腎醒腦湯) led to the high efficacy.

• The Journal of Korean Medical History
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• v.30 no.2
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• pp.1-12
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• 2017

This paper is focused on the 'contents' of database level medical texts sponsored by the Chinese government. The premise of the study is that the contents of state-sponsored medical texts would show how medical policy makers and practitioners approached the body and diseases of the time, and by association the medical text would reveal the policy associated with state medical education and distribution of medical resources associated with the practitioners' approaches. This paper analyzes the contents of four representative state-sponsored medical texts: Cao's Treatise on the Origins and Symptoms of Various Diseases (巢氏諸病源候論, 610, Sui China); Great Peace and Sagely Benevolence Formulas (太平聖惠方, 996, Song China); Complete Record of Sagely Benevolence (聖濟總錄, 1117, Song China); Formulas for Universal Relief (普濟方, 1406, Ming China).

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.3
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• pp.1193-1196
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• 2014

Senescence marker protein 30 (SMP30), a hepatocellular carcinoma (HCe) associated antigen had been identified by our research group. To study its mechanisms of regulation and associations with the occurrence and development of HCe, we inhibited expression by RNAi technique, and observed effects on the biological characteristics of Hep G2 cells. In cell viability assays, cell growth in the experimental group (with siRNA transfection) was elevated. In Transwell invasion assays, compared with blank and control groups, numbers of invading cells in the experimental group were significantly increased, whereas in apoptosis assays, the percentage apoptosis demonstrated no differences, but after UV irradiation, that in the experimental group was higher than the other two groups. In a word, SMP30 can inhibit the proliferation and invasion of human hepatoma cells and thus can be regarded as a cancer suppressive factor.

• IMMUNE NETWORK
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• v.23 no.4
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• pp.34.1-34.15
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• 2023

Lung cancer, particularly non-small cell lung cancer (NSCLC) which contributes more than 80% to totally lung cancer cases, remains the leading cause of cancer death and the 5-year survival is less than 20%. Continuous understanding on the mechanisms underlying the pathogenesis of this disease and identification of biomarkers for therapeutic application and response to treatment will help to improve patient survival. Here we found that a molecule known as DUSP10 (also known as MAPK phosphatase 5) is oncogenic in NSCLC. Overexpression of DUSP10 in NSCLC cells resulted in reduced activation of ERK and JNK, but increased activation of p38, which was associated with increased cellular growth and migration. When inoculated in immunodeficient mice, the DUSP10-overexpression NSCLC cells formed larger tumors compared to control cells. The increased growth of DUSP10-overexpression NSCLC cells was associated with increased expression of tumor-promoting cytokines including IL-6 and TGFβ. Importantly, higher DUSP10 expression was associated with poorer prognosis of NSCLC patients. Therefore, DUSP10 could severe as a biomarker for NSCLC prognosis and could be a target for development of therapeutic method for lung cancer treatment.

• International Commerce and Information Review
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• v.17 no.3
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• pp.93-114
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• 2015

In this study, the national competitiveness of Korean and Chinese medical device industry was analyzed through RCA index, MCA index, and TSI index in order to draw the export specialized items possessing the national competitiveness of Korea in Chinese market. The drawn national competitiveness index was grouped and the change progress was examined by years. Korea should analyze the tendency of Chinese medical device market, confirm our market share in the Chinese market, apprehend the competitive superiority, and draw and excavate the specialized export item in order to strengthen the competitiveness of Korean medical device in the Chinese market.

• Journal of Korean Medical classics
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• v.23 no.3
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• pp.81-101
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• 2010

The results of researching Jang's use of herb remedies through his book "Uihakchungjungchamseorok(醫學衷中參西錄)" are as follows. 1. Jang Seoksun's medicine is based on "Hwangjenaegyeong(黃帝內經)", "Sanghanron(傷寒論)", "Sinnongbonchogyeong(神農本草經)". And through constant study he brought to perfection his own unique medical theory. 2. He introduced Western medicine, and united it with traditional Chinese medicine. He perfected the Chinese-Western medical theory, by Chungjungchamseo(衷中參西) which means 'roots in traditional Chinese medicine, reference in Western medicine'. 3. He thought Onbyeong(溫病) was not an independent category of disease, but included it into the category of Sanghan(傷寒). So he used modified prescription of "Sanghanron(傷寒論)" to treat Onbyeong(溫病). 4. He expanded the category of remedy uses by using various compounds, such as minerals and animal compounds. He has also developed substitute remedies.

• The Journal of Korean Medical History
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• v.25 no.1
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• pp.1-4
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• 2012

Dysuria has been mentioned several times in "Treatise on cold" and "Golden Chamber" of Zhang Zhong-jing in Han Dynasty. This article makes a detailed discussion of etiology, pathogenesis, treatment and prescription of Dysuria from three periods that is Han and Tang Dynasty, Song and Yuan Dynasty, Ming and Qing Dynasty. It has demonstrated the inheritance, development and innovation of academic ideas of later physicians from Zhang Zhong-jing, which enriched and developed the theory of Zhong-jing.

• Korea Journal of Hospital Management
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• v.20 no.1
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• pp.25-38
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• 2015

China has a growing number of medical tourists to visit South Korea every year, with economic growth, and thus Korea's medical tourism is growing countries in the new growth engine industries and attracting attention. In this study, this time looking at the risks and benefits of medical tourism potential factors consumers feel the Chinese people for South Korea medical tourism, economic, based on existing research, functional, temporal, social, and psychological setting factors, risks and benefits conducted a study of the relationship between the purchase of your help. Risk factors identified through this study, we investigate expanding the overseas medical tourism market to visit Korea by managing the benefits and know the strategic implications that can achieve customer satisfaction by the service convenient for Chinese tourists.

• Korea Journal of Hospital Management
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• v.12 no.4
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• pp.45-67
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• 2007

This study is focused on the medical services market in china which would be the largest one in the world sooner or later. An empirical research has been performed on the country images and related buying attitudes of the Chinese potential consumers for foreign medical services of more higher level. Upon the basis of this research results, the components of a country image has been restructured and the country image effects on the process of a purchasing decision of the advanced foreign medical services in China has been investigated and analyzed. This research shows that the forming process and the dimensions of a country image in Chinese consumers are rather simplified than the former researches of the same kind in any other countries. In China the expectation and buying intension for foreign medical services is found to be affected directly by a country image. Furthermore among various components of a country image the expected service quality level of the Chinese is found to be mostly dependent on the social stability and safety rather than on the degree of economic developments. Recently breaking through the domestic medical market crisis, more and more hospitals consider to advance into Chinese medical market. This research shows that the reexamination and political concerns on the country image of Korea are needed in the level of government's public relations. Especially the proactive policy making and propaganda of political, social and economic stability and safety in Korea are thought to be more important for successful entry in Chinese medical services market.