• Nutrition Research and Practice
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• 제5권3호
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• pp.253-259
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• 2011

The rapid rise in the incidence of obesity has emerged as one of the most pressing global public health issues in recent years. The underlying etiological causes of obesity, whether behavioral, environmental, genetic, or a combination of several of them, have not been completely elucidated. The obesity epidemic has been attributed to the ready availability, abundance, and overconsumption of high-energy content food. We determined here by Pearson's correlation the relationship between food type consumption and rising obesity using the loss-adjusted food availability data from the United States Department of Agriculture (USDA) Economic Research Services (ERS) as well as the obesity prevalence data from the Behavioral Risk Factor Surveillance System (BRFSS) and the National Health and Nutrition Examination Survey (NHANES) at the Centers for Disease Control and Prevention (CDC). Our analysis showed that total calorie intake and consumption of high fructose com syrup (HFCS) did not correlate with rising obesity trends. Intake of other major food types, including chicken, dairy fats, salad and cooking oils, and cheese also did not correlate with obesity trends. However, our results surprisingly revealed that consumption of com products correlated with rising obesity and was independent of gender and race/ethnicity among population dynamics in the U.S. Therefore, we were able to demonstrate a novel link between the consumption of com products and rising obesity trends that has not been previously attributed to the obesity epidemic. This correlation coincides with the introduction of bioengineered corns into the human food chain, thus raising a new hypothesis that should be tested in molecular and animal models of obesity.

• Preventive Nutrition and Food Science
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• 제7권4호
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• pp.373-377
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• 2002

Allergens in processed foods may place persons with food allergies at significant risk when the labels do not Provide sufficient warnings or identification of high-risk ingredients. Because egg proteins are common food allergens, this study was carried out to identify hen's egg albumin (ovalbumin, OVA) in five commercially processed foods containing egg (custayd, cookie and pasta), and chicken meat (sausage and meatball) by immunological methods using commercially produced murine monoclonal immunoglobulin G (M-IgG), immunoblotting and enzyme linked immunosorbent assay (ELISA). Sample buffer with chelating and reducing agents was prepared and used for the preparation of the protein fractions from the foods. Most bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profile (5~15% gradient gel) presented at 75 kDa below. OVA (43 kDa) in the sample lanes could not be visually observed on the gel. However, OVA in solutions prepared from custard and cookie could be detected by M-IgG, but were not detected in sausage and pasta. OVA in all samples could be quantitatively determined by the equation obtained from the standard curve by ELISA. Cookie and custard containing egg white and egg, respectively, contained very high concentrations of OVA. OVA in the other products were present in relatively low concentrations, but sufficiently high to pose possible risk of allergy, ELISA is a very sensitive and precise method for the identification and quantification of allergens in food products including allergy-inducible materials.

• 한국식품위생안전성학회지
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• 제8권4호
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• pp.215-223
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• 1993

클로람페니콜은 광벙위 항생물질로서 수의축산분야에서 널리 사용되어 왔으나 이 제제의 잠재적인 독성과 축산식풍 중 잔류성 때문에 세계 각국에서는 식용동물에서의 사용을 금지하고 있다. 따라서 이 시험에서는 미생물학척 방법에 의한 우유 및 식육 중 미량의 잔류클로람페니콜을 측정할 수 있는 방법을 확립하고자 M. luteus ATCC 9341을 시험용 균주로 설정하여 다음과 같은 결과를 얻었다. 1. 검출감도를 향상시키고자 시험명판용 Mueller-Hinton 배지의 배지량을 최소화하고 ethylacetate로 추출 농축하여 ml당 0.15/lg의 옥시테트라사이클린을 첨가한 회석용 인산염 완충액 (pH 6.0) 으로 잔류물을 용해하여 사용하였을 때 가기장 좋은 검출강도를 나타내였다. 2. 시료별 최저검출농도는 원유가 0.025 ppm이었으며, 식육은 0.05 ppm까지 검출할 수 있었다. 3. 시료별 평균 회수율은 원유가 68.5%이었으며, 쇠고기, 돼지고기 및 닭고기는 각각 65.1%. 63.8% 및 59.4%이었다. 이들 시료의 회수율에 대한 변이계수 (CV)는 1.8~15.1%이었다.

• 한국축산식품학회지
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• 제32권2호
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• pp.162-167
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• 2012

Phosvitin was extracted from a chicken egg yolk and the iron-binding, along with antioxidative activity of the extracted phosvitin, was determined after mixing with ground beef at the concentrations of 100 and 500 mg/kg of meat. The electrophoretic pattern of the extracted phosvitin on SDS-PAGE was found to be identical to that of the standard phosvitin. The extracted phosvitin at $1,000{\mu}g$/mL showed an ability to bind approximately 65% of the iron in a 3 mM iron solution. Lipid oxidation was inhibited in the ground beef mixed with 500 mg/kg of the extracted phosvitin, during storage at $4^{\circ}C$ compared to that of the control (p<0.05). Additionally, color stability of ground beef containing the extracted phosvitin was enhanced (p<0.05). The pH, cooking loss, texture, and sensory properties of the ground beef were not affected, by adding up to 500 mg/kg of the extracted phosvitin. This result suggests that the phosvitin extracted from egg yolk could be used as an antioxidant reagent. In particular, phosvitin would be more amenable for use in meat products because it is a natural protein derived from animal products.

• Applied Biological Chemistry
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• 제47권2호
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• pp.197-201
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• 2004

식품 가공의 부산물 중 동물성 부산물을 재활용하여 조미소재로의 개발 가능성을 탐색하였다. 부산물 원료로는 돼지뼈(PB)와 닭뼈(CB), 그리고 참치 혈합육(TDF)을 사용하였다. PB와 CB를 열수로 18시간 추출한 후 단백질과 지방 분해효소를 처리하여 가수분해물을 제조하였다. 각각의 열수 추출물에서 지방의 제거를 위하여 1차로 lipase활성이 있는 pancreatic enzyme을 처리하고 2차로 Flavourzyme을 사용하는 경우 가수분해도(DH)가 PB는 약 70%, CB는 약 80%까지 증가하였다. 지방이 적은 TDF는 단백질 분해효소인 Alcalase와 Flavourzyme으로 분해한 결과 고형분 회수율은 약 22%, 단백질 회수율은 9%이었으며, 효소분해액 중 유리 아미노산 함량은 고형분 중 27%까지 향상되었다. 세 종류 분해액에 가염한 후 관능적인 특성을 조사한 결과 전체적으로 TDF>PB>CB의 순으로 우수하여 참치 혈합육은 조미소재의 원료로 적합한 동물성 부산물로 판단되었다.

• 한국환경농학회지
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• 제40권2호
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• pp.127-133
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• 2021

BACKGROUND: Dithiocarbamte fungicides have been used in crop cultivation for diseases protection and treatment. And cultivated agricultrual products were used as feedstuff, and residual pesticides are likely to be absorbed and transferred to livestock. But the maximum residue limits (MRLs) were not established for dithiocarbate fungicides in livestock products, and thus an analysis method was developed and validated for dithiocarbamate fungicides to establish MRLs. METHODS AND RESULTS: Samples were prepared using CS₂ trap method and detected with UV/VIS spectrophotometer. Calibration line (0.1 ~ 10 ㎍/mL) was linear with r² > 0.99. For validation, the recovery tests were carried out at three fortification levels (MLOQ, 10 MLOQ and 50 MLOQ) from livestock samples (egg, milk, beef, pork, and chicken). The results for mancozeb, propineb, and thiram ranged between 76.8 to 109.6%, 79.4 to 108.8%, and 80.2 to 107.8%, respectively and % RSD (relative standard deviation) values were below 9.5%. Furthermore, inter-laboratory analysis was performed to validate the method. CONCLUSION: All values were corresponded with the criteria ranges requested by both the CODEX (CAC/GL 40-1993, 2003) and MFDS guidelines (2016). This might be used as an official analytical method for determination of dithiocarbamate fungicides at established MRLs and monitoring.

• 농업과학연구
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• 제46권2호
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• pp.361-368
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• 2019

The objective of the current study was to determine calcium (Ca) and phosphorus (P) utilization in 4 different hatchery by-products (HBPs) for broiler chickens. The four different HBPs included infertile eggs (IFE), unhatched eggs (UHE), low grade and dead chicks (LDC), and a mixture (MIX) of 55% IFE, 10% UHE, and 10% LDC with 25% hatched eggshells. A total of sixty four 50-day-old Ross 308 broiler chickens were randomly allotted to 1 of 4 dietary treatments with 8 replicates per treatment. Two birds were placed together in one metabolic cage. Additional 16 birds were used to measure the endogenous losses of Ca and P. A forcefeeding procedure (i.e., crop intubation) was used to measure the apparent and true total tract retention (ATTR and TTTR, respectively) of Ca and P in the 4 HBPs. The results showed that the TTTR of Ca in the UHE was less (p < 0.05) than that in the IFE, LDC, and MIX. The amounts of available Ca in the MIX were greater (p < 0.05) than those in the IFE and UHE, which were greater (p < 0.05) than those in the LDC. The TTTR of P was not different among the 4 HBPs. However, the amounts of available P in the LDC were greater (p < 0.05) than those in the IFE and UHE, which were greater (p < 0.05) than those in the MIX. In conclusion, HBPs contain high amounts of available Ca and P because of high concentrations of total Ca and P with a high utilization rate. Therefore, the use of HBPs in broiler diets can reduce costs on Ca and P supplements.

• 한국포장학회지
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• 제21권3호
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• pp.97-106
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• 2015

RTE(ready-to-eat)형 전자레인지 가열용 제품들의 가열 조리 시 특정 부위의 열 변형이 발생하여 전자레인지 포장재의 재질 및 형태별 가열 패턴을 조사하였다. 국내에서 유통되고 있는 시료 9종 중 5개의 시료에서 열 변형이 발생되었고, 국외에서 유통되고 있는 시료 11종 중 8종에서 열 변형이 발생되었다. 국외 시료의 경우 국내 시료보다 열 변형 정도는 덜 하였지만 일부 발생되는 것을 확인하였다. 또한 트레이 타입 용기에 열 변형이 발생하는 소스를 담고 전자레인지로 가열하였을 때도 리드지 및 컵에서 열 변형이 관찰되었다. 국내외에서 유통되고 있는 전자레인지 가열용 제품의 염도, 당도, pH 및 점도를 측정한 결과 열 변형 발생 유무 및 정도에 따른 일정한 경향은 없었다. 하지만 극히 낮거나 높은 점도를 가진 시료에서 열 변형이 나타나지 않았고 내용물의 형태 및 포장재의 형태에 따라 열 변형의 차이가 있었다. 따라서 제품의 열 전환 및 열전달을 고려하여 포장재의 깊이 등 설계를 반영하여 포장 내 전자파의 집중을 방지하는 등의 기술 도입이 필요할 것으로 판단된다.

• 품질경영학회지
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• 제50권2호
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• pp.287-304
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• 2022

Purpose: The size of the salad consumption market has expanded since Covid-19, and continuous growth is predicted. Therefore, by extracting influential core purchasers in the salad consumption market and analyzing their purchasing behaviors and consumer types, this study intended to provide basic data for establishing a marketing strategy. Methods: The analysis data is the purchasing data of 576 people who have purchased salads between 2016 and 2020 (panel data of the Rural Development Administration), and in the social network analysis, the centrality structure was analyzed. Results: First, in the results of analyzing the causes of the rapid increase in salad consumption in 2020, it was found that the increase in consumption of new purchasers (n=102) had little effect. The existing consumer type (n = 474), which has been the majority of the salad consumption market so far, were consumers with stable income. However, the results of study indicated that the type of consumers has expanded since low-income class as well as high-income class increased consumption of purchasing salad. Second, in the results of analyzing the types of key purchasers with great influence in the salad consumption market, there was a difference from the results of frequency analysis in age, number of family members, existence/absence of children, and income decile. This suggests that there should be a difference between the type of customers according to the apparent quantitative figure and the actual influential purchasers. Third, in the results of analyzing the salad purchasing behaviors of core purchasers, the purchasing site for existing purchasers was large-scale marts and for new purchasers it was corporate-type supermarkets. Purchases were concentrated on Saturdays for both existing and new purchasers. As for the purchased products, existing purchasers had a high preference for products made of chicken, and new purchasers had a high preference for vegetable/fruit salad. In particular, in the results of purchased products by age group, in the case of 50s and 60s, it was an interesting result that there was a difference between the products purchased by the existing and new purchasers even though they were the same age. Conclusion: When establishing a marketing strategy in the salad consumption market, it is necessary to pay attention to the purchasing behavior of key buyers.

• Animal Bioscience
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• 제36권4호
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• pp.654-670
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• 2023

Objective: This study aimed to develop loop-mediated isothermal amplification (LAMP) combined with lateral flow dipstick (LFD) and compare it with LAMP-AGE, polymerase chain reaction (PCR), and standard Salmonella culture as reference methods for detecting Salmonella contamination in animal products and animal production environmental samples. Methods: The SalInvA01 primer, derived from the InvA gene and designed as a new probe for LFD detection, was used in developing this study. Adjusting for optimal conditions by temperature, time, and reagent concentration includes evaluating the specificity and limit of detection. The sampling of 120 animal product samples and 350 animal production environmental samples was determined by LAMP-LFD, comparing LAMP-AGE, PCR, and the culture method. Results: Salmonella was amplified using optimal conditions for the LAMP reaction and a DNA probe for LFD at 63℃ for 60 minutes. The specificity test revealed no cross-reactivity with other microorganisms. The limit of detection of LAMP-LFD in pure culture was 3×10² CFU/mL (6 CFU/reaction) and 9.01 pg/μL in genomic DNA. The limit of detection of the LAMP-LFD using artificially inoculated in minced chicken samples with 5 hours of pre-enrichment was 3.4×10⁴ CFU/mL (680 CFU/reaction). For 120 animal product samples, Salmonella was detected by the culture method, LAMP-LFD, LAMP-AGE, and PCR in 10/120 (8.3%). In three hundred fifty animal production environmental samples, Salmonella was detected in 91/350 (26%) by the culture method, equivalent to the detection rates of LAMP-LFD and LAMP-AGE, while PCR achieved 86/350 (24.6%). When comparing sensitivity, specificity, positive predictive value, and accuracy, LAMP-LFD showed the best results at 100%, 95.7%, 86.3%, and 96.6%, respectively. For Kappa index of LAMP-LFD, indicated nearly perfect agreement with culture method. Conclusion: The LAMP-LFD Salmonella detection, which used InvA gene, was highly specific, sensitive, and convenient for identifying Salmonella. Furthermore, this method could be used for Salmonella monitoring and primary screening in animal products and animal production environmental samples.