• Title/Summary/Keyword: chicken egg

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Histological Change of Uterus Endometrium and Expression of the Eggshell-related Genes according to Hen Age (닭의 산란연령에 따른 자궁내막조직의 변화 및 난각 관련 유전자의 발현양상)

  • Park, Ji Ae;Cho, Eun Jung;Park, Jung Yeon;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.44 no.1
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    • pp.19-28
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    • 2017
  • The eggshell is an intricate and highly ordered structure composed of multiple layers and a calcified matrix. The eggshell is formed at the uterine segment of the chicken oviduct. In this study, histological changes in the uterine endometrium and the expression of the eggshell-related genes were investigated according to hen age. We analyzed the expression of eggshell protein-related genes, such as OCX-32, OCX-36, OC-17, OC-116, and eggshell-ion-related genes, such as CABL-1, SPP1, SCNN1G, ATP2A2, CA2, and CALM1. In chicken uterine endometrium, histological deformation, fibrosis, atrophy and elimination of micro-villi were found with increasing hen age. The concentration of blood-ion components did not significantly change with age. The amount of telomeric DNA in uterine endometrial cells decreased with increasing hen age. The expression of most of the eggshell-related genes changed significantly with increasing hen age. The expression of some ovo-proteins, which play a role in eggshell formation, increased with increasing hen age; however, there were no significant correlations among eggshell protein genes. Eggshell ion-related genes, such as ATP2A2, SCNN1G, CA2, and CALM1, were closely related to each other. The OCX-32 and OCX-36 genes were closely related to some of the eggshell ion genes. Eggshell protein-related genes, such as the OCX-32, OCX-36 genes and ion-related genes such as CALB-1, ATP2A2, SCNN1G, CA2, CALM1, affected eggshell formation, mutually or independently. This study shows that, uterine although endometrial cell damage occurs with increasing hen age, normal eggshells can be formed in old hens. This suggests that eggshell protein-and eggshell ion-related genes also control the homeostasis of eggshell formation.

Studies on Prevalence of Meat Allergy and Potential Beef Allergens (식육알레르기 발생 경향 및 잠재적 우육알레르겐에 관한 연구)

  • Jeong, Bo-Young;Kim, Dong-Yeop;Fan, Jiang Ping;Chung, Hyun-Chae;Han, Gi-Dong
    • Food Science of Animal Resources
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    • v.29 no.2
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    • pp.151-156
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    • 2009
  • The prevalence of food allergies was investigated using questionnaires with 300 subjects whose ages ranged from 19 to 24 years old and the causative food allergens was analyzed using immunological analysis with serum of the subjects who answered that they have/had food allergy. The questionnaire showed that 11.33% of subjects have/had experience of food hypersensitivity, where the main causative foods were fish, beef, chicken, milk, egg, and pork in order. The meat allergy shared 4.65% (2.33% for beef, 1.66% for chicken, 0.66% for pork) in the prevalence of food allergies. The causative beef allergens were investigated with the serum of 6 subjects who have had beef allergy. Western blots were carried out with the serum of P6 subject who showed a positive reaction to beef extract in ELISA. The two specific bands were detected in beef extract on the PVDF membrane, and no band was detected in extracts of pork and chicken. A calculation of the distance of migration by SDS-PAGE enabled the molecular masses of the two bands to be estimated as 67kDa and 31kDa, respectively. The 67kDa was revealed as bovine serum albumin (BSA) which is one of the important beef allergens as reported previously though an analysis of the N-terminal amino acid sequence. However we could not identify the sequence of 31kDa, probably because they comprised several subunits and were modified proteins such as glycoprotein that were unlikely to be easily degraded by the Edman method. The 31kDa band were dyed with the PAS (periodic acid-schiff reagent), suggesting that it might be a glycoprotein. These results suggested that the 31kDa might be considered as a novel potential beef allergen which is not reported previously, although further studies are needed.

Cryopreservation of Primordial Germ Cells(PGCs) from Korean Native Chicken(Ogye) Embryos using Commercial Cryoprotectants (상업용 동결보호제를 이용한 한국재래닭(오계) 원시생식세포의 동결 보존)

  • Kim, Hyun;Kim, Dong Hun;Han, Jae Yong;Do, Yoon Jung;Kim, Jae Hwan;Kim, Young Sin;Seong, Hwan Hoo;Ko, Yeoung Gyu;Kim, Sung Woo
    • Korean Journal of Poultry Science
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    • v.40 no.3
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    • pp.163-169
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    • 2013
  • Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the unique anatomical and physiological characteristics of the avian egg. Thus an alternative strategy for conservation of oviparous species of animals must be developed. Recent technological developments for producing germline chimeras by the transfer of primordial germ cells (PGCs) into recipient embryos has enabled the conservation and retrieval of chicken genetic resources in their complete form. In the present study, fertilized eggs were incubated for about 5.5 days to obtain embryos at stage 28. The whole embryo was collected from the germinal gonad using a fine glass micro pipette under a microscope. The PGCs were then purified using MACS method. Two commercially available cryoprotectants (A and B) were used to preserve the PGCs, and EG were used as a control. The average recovery rate of PGCs after thawing was 35.5% and 60.5% with the A and B treatments, respectively. There was no significant difference between B treatments and control, which showed an average recovery rate of 52.8%. However, the recovery rate obtained using A cryoprotectant (35.5%) was significantly lower than using treatment control and B. The average viability of the PGCs after thawing were 77.9% and 77.4% for cryoprotectants A and B, respectively, and the control were was 81.6%. There was no statistically significant difference between the two treatments and control. It was concluded that all of the available cryoprotectants examined in this study could be used for preservation of PGCs from embryos. Further experiments to produce germline chimera from PGCs preserved using this techniques are strongly recommended.

Comparison of a PCR Kit and a Selective Medium to Detect Pathogenic Bacteria in Eggs (PCR Kit와 선택배지를 이용한 계란의 병원성세균 검출 비교 평가)

  • Kim, Dong-Ho;Yun, Hye-Jeong;Song, Hyun-Pa;Lim, Sang-Yong;Jo, Min-Ho;Jo, Cheo-Run
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.965-970
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    • 2009
  • PCR technology has been widely used to detect and quantify microbial pathogens in foodstuffs, because the technique is rapid, sensitive, and selective. In this study, detection of contaminating pathogenic bacteria on shells of chicken eggs was performed using both a commercial multiplex polymerase chain reaction (PCR) kit and a viable count method employing a selective medium. The PCR kit was capable of detecting Campylobacter jejuni, Escherichia coli O157:H7, Staphylococcus aureus, Bacillus cereus, Vibrio parahaemolyticus, Listeria monocytogenes, Yersinia enterocolitica, Salmonella species, and Shigella species. Using the PCR method, five bacterial species were detected from 30 samples (33.3%) of 90 batches of eggs commercially available in a market. PCR products from B. cereus, S. aureus, L. monocytogenes, Y. enterocolitica, and E. coli O157:H7 were detected, and the numbers and frequencies of positive samples were 17 (18.8%), 12 (13.3%), 15 (16.6%), 16 (17.7%),and 4 (4.4%), respectively. None of any Salmonella species, C. jejuni, V. parahaemolyticus, or Shigella species was detected in this study. The results of PCR testing were confirmed using a typical viable count method employing a selective medium. We suggest that the multiplex polymerase chain reaction (mPCR) assay is a rapid and reliable method for detection of pathogenic bacteria contaminating eggs.

Analysis of Fluoroquinolone Antibiotics in Foods (식품 중 플루오로퀴놀론계 항생제의 분석)

  • Kim, Hee-Yun;Shin, Min-Su;Choi, Hee-Ju;Park, Se-Jong;Song, Jae-Sang;Cheong, So-Young;Choi, Sun-Hee;Lee, Hwa-Jeong;Kim, Young-Seon;Choi, Jae-Chun
    • Korean Journal of Food Science and Technology
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    • v.41 no.6
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    • pp.636-643
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    • 2009
  • Residual fluoroquinolone levels in animal foods retailed in Korea were monitored according to the method outlined in Korea Food Code using HPLC-FLD and HPLC-ESI-MS/MS for confirmation. The optimum ion transitions were $360{\rightarrow}316$, 342 m/z for enrofloxacin, $332{\rightarrow}314$, 288 m/z for ciprofloxacin, $320{\rightarrow}301$, 230 m/z for norfloxacin, $334{\rightarrow}315$, 290 m/z for pefloxacin, $362{\rightarrow}318$, 261, 334 m/z for ofloxacin, and $262{\rightarrow}201$, 126 m/z for flumequin. Enrofloxacin, ciprofloxacin and norfloxacin residues were found in 12 out of 388 samples. These antibiotics were only found in chicken samples, while no residues were found in beef, pork, milk and egg samples. Using this monitoring method, detection rates of 3.1, 1.3, and 0.3% were obtained for enrofloxacin, ciprofloxacin and norfloxacin, respectively. The levels of enrofloxacin, ciprofloxacin and norfloxacin detected in food samples ranged from 0.01 to 0.73 mg/kg in 12 samples, 0.01-0.03 mg/kg in 5 samples, and 0.12 mg/kg in only a sample, respectively.

Biological Probiotic Properties of Lactobacillus rhamonosus GG-4 Isolated from Infant Feces (유아분변으로부터 분리한 Lactobacillus rhamonosus GG-4의 생균제적 특성)

  • Kang, Jin-Hae;Kim, Dae-Hwan;Lee, Sang-Won;Kim, Hong-Chul;Cho, Young-Un;Gal, Sang-Wan
    • Journal of Life Science
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    • v.20 no.12
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    • pp.1882-1888
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    • 2010
  • To develop probiotics, a kind of Lactobacillus sp. was isolated from infant feces. The bacterium was identified as Lactobacillus rhamnosus through 16S rDNA sequence analysis. The strain was a facultative anaerobe which grew better in aerobic conditions. The bacterium lowered the pH of the culture solution down to 2.4 during 48 hr in the MRS medium. The strain inhibited the growth of 6 pathogens - S. aureus, L. monocytogens, S. typhimurium, E. coli O-157, V. parahaemolyticus and P. aeruginosa. When the Lactobacillus were fed to chickens, along with commercial feed, for one month, amounts of $H_2S$ and $NH_3$ in the feces of the chicken decreased to 50% and 70%, respectively, compared to those of control group chickens. Amounts of other bad smells such as $(CH_3)SH$, $(CH_3)_2S$ and $(CH_3)_2S_2$ were not much different in the Lactobacillus-fed chickens compared to the control group. On the other hand, egg weights of the chickens fed Lactobacillus were higher by about $5{\pm}1\;g$ than those in the control group.

The Performance Test in Crossbreds of Korean Native Chickens for the Establishment of New Lines (새로운 계통 조성을 위한 한국 토종닭 교배 조합의 능력 검정)

  • Lee, Myeong-Ji;Heo, Kang-Nyeong;Choi, Hee-Cheol;Hong, Eui-Chul;Kim, Chong-Dae
    • Korean Journal of Poultry Science
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    • v.41 no.1
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    • pp.39-44
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    • 2014
  • The goal of this study was to evaluate the growth performance of crossbred strains using Korean native chicken (KNC). The data were collected from 2012 through 2013 at Poultry Science Division, National Institute of Animal Science, Korea. The number of chicks analyzed in this study was 375. Crossbred were (A) $R{\times}S$, (B) $D{\times}H$, (C) $D{\times}S$, (D) $C{\times}Y$ and (E) $Y{\times}H$. The fertility rates of strains were 94.9% in crossbred A, 91.9% in crossbred B, 91.8% in crossbred C, 89.7% in crossbred D and 93.8% in crossbred E, respectively. The fertility was highest in crossbred A, but crossbred C showed the lowest (p<0.05) based on the hatchability. The crossbreds B and C exhibited a superior performance on body weight gains during growing stages but crossbred D showed the lowest (p<0.05). The feed intake was to the pattern of body weight. The feed intake of crossbred D was significantly lower than other crossbreds. Also, feed conversion ratio of crossbred D showed the lowest (p<0.05). Egg production ratio of crossbred D was significantly higher compared to the other crossbreds. These results suggest that the development of new crossbreds of commercial Korean Native Chickens should be required for better performance.

Investigation on Status of Pou1try Industry in Japan (일본의 양계추이와 경영실태에 관한 조사분석)

  • 오세정
    • Korean Journal of Poultry Science
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    • v.16 no.1
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    • pp.29-52
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    • 1989
  • Selection of breeds, adequate environment and sanitary condition must be considered to improve production ability. Minimizing of production cost through reasonable management and farm system can introduce maximizing net income. Followings are the summary of poultry industry in Japan: (1) To decreasing consumers Price and to gurantee the quality of eggs, poultry farms changed their farm system as complex which contain feed factory, GP center and processing factory as well as raising laying hens. (2) Broiler farms moved from suburbs to several remote from cites owing to development of transferation , and rising price of land. (3) The egg Price in Japan was depended on season. From fall to spring, the Price was usually rised due to increased consumption, and from May to August the price always dropped. (4) The average production per one you was around 17kg/hen and average feed consumption was around 2.45. Rearing rate was around 98.5% and motality of hens was around 7%. And one person reared 15,000-20,000 hens in over 100 thousand chicken reared farms even though average ability per person showed 3,266 hens.

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A Study on the Preference on Protein Rich Foods in Kindergarten Children in Gyeong Nam Area (단백질(蛋白質) 급원식품(給源食品)을 기피(忌避)하는 일부(一部) 유치원(幼稚園) 아동(兒童)의 식이형성(食餌形成)과 그 요인(要因))

  • Yoon, Hyun-Sook
    • Journal of Nutrition and Health
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    • v.17 no.1
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    • pp.3-9
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    • 1984
  • Preference on protein rich foods of 103 children, aged 4 to 6, of kindergarten located in Masan Jin-hea, Chang-won cities, was conducted April 25 to May 4, 1983. The results are summarized as follows ; 1) Seventy six percent of the subjects were from families having two three children, without grandmothers in the home About 43% of mother had completed high school, 37% had completed middle school. Mothers' ages ranged from 26 to 50 years, with 57.3% in the 31-40 age bracket. About 63% of the fathers were office and government employees, while 77.6% of mothers were unemployed. 2) Over 50% of the children disliked or refused pork, thick beef soup, liver, soybean, oyster, clam, anchovy, croaker, mackerel, loach, hairtail, porgy, flatfish, a walleye, pollack, a dried walleye pollack, a sciaenoid fish. Taste bad, the characteristic fragrance and lack of experience were the main reasons why the children refused these foods. Over 33% of the children were unexperienced liver, ribs of beef. the small intestine of cattle, a loach, an eel. Over 50% liked milk, egg, dried filefish, sausage, a cattlefish, beef, chicken, a crab, shrimp, bean curd. 3) Most of children hoped that their daily meals should be made in prettier, more sabory, and various ways. Mothers also hoped to have practical knowledge of nutrition and its influence on our body.

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Risk Assessment for Heavy Metals in Korean Foods and Livestock Foodstuffs (한국인의 대표식품 및 축산식품에 대한 중금속 위해도 평가)

  • Kwon, Young-Min;Lee, Kyoung-Hee;Lee, Haeng-Shin;Park, Seon-Oh;Park, Jung-Min;Kim, Jin-Man;Kang, Kyung-Mo;No, Ki-Mi;Kim, Dong-Sul;Lee, Jong-Ok;Hong, Moo-Ki;Choi, Dal-Woong
    • Food Science of Animal Resources
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    • v.28 no.3
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    • pp.373-389
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    • 2008
  • This study was conducted to evaluate exposure level and risk of heavy metals in livestock foodstuffs and Korean foods. Based on the "Food Intake Data," a part of the 2005 National Health & Nutrition Survey and the "2005 Seasonal Nutrition Survey", 113 Korean foods items were selected. 3 samples from different manufacturers of each 113 items of Korean foods were purchased on summer and fall, so total 678 samples were used. The food groups were classified into 15 categories. For the livestock foodstuffs category, meats and poultry (chicken, pork, pork belly, beef, beef feet soup), milks and dairy products (milk, ice cream, liquid yoghourt, sherbet), eggs (egg) were selected. It was found that the daily amount of heavy metals intake (mg/person/day) from livestock foodstuffs is 0.00020 arsenic, 0.00000 cadmium, 0.00020 lead, and 0.00006 mercury, and the daily amount of heavy metals intake (mg/person/day) from Korean foods is 0.0265 arsenic, 0.0083 cadmium, 0.0067 lead, and 0.0028 mercury. Daily amount of heavy metals intake from livestock foodstuffs was low among the food groups. For risk assessment, PDI (Probable Daily Intake) was calculated and compared with PTWI (Provisional Tolerable Weekly Intake) of JECFA (Joint FAO/WHO Expert Committee on Food Additive). Relative hazard of these livestock foodstuffs was 0.006% in arsenic, 0.000% in cadmium, 0.085% in lead, and 0.149% in mercury. Relative hazard of Korean foods was 0.941% in arsenic, 14.676% in cadmium, 3.319% in lead, and 6.860% in mercury. Thus, livestock foodstuffs and Korean foods were as safe as satisfied with the recommended standards of JECFA.