• 한국어병학회지
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• 제13권1호
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• pp.31-36
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• 2000

양식 농어에 있어서 연속 채혈 스트레스는 혈장내 glucose 농도의 뚜렷한 증가 및 말초 혈액내 식세포의 chemiluminescent 반응을 유의적으로 감소시켰다. 실험어는 연속적인 스트레스에 대해 축적적인 반응을 나타내었으며, 혈장내 glucose의 농도는 스트레스 부과 시간간격에 의존하는 것으로 나타났다. 혈장내 glucose의 농도와 chemiluminescent 반응간에는 통계적으로 유의적인 (P<0.05) 음의 상관관계를 나타내었다.

• Fisheries and Aquatic Sciences
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• 제3권1호
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• pp.23-25
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• 2000

To compare the respiratory burst activity potential of the phagocytes isolated from head kidney, spleen, and peripheral blood 1ll cultured rockfish (Sebastes schlegeli), chemiluminescent (CL) response analysis was performed. The phagocytes isolated from peripheral blood showed greater and faster CL response to the opsonized zymosan compared to that of the phagocytes isolated from kidney or spleen. This may imply a significant role of the blood phagocytes in defence mechanism of rockfish. The different responses found in the CL analysis among the phagocytes isolated from peripheral blood, kidney, and spleen may reflect differences in activation state or activity of phagocytes.

• 한국어병학회지
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• 제13권2호
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• pp.137-142
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• 2000

간흡충 탈낭유충에 대한 잉어, 붕어, 참붕어의 보체 살충능 및 식세포 chemiluminescent 반응 차이를 조사하였다. 낙동강에서 채집한 참붕어는 모두 간흡충 피낭유충에 중감염되어 있었으나, 진영의 저수지에서 채집한 참붕어 및 낙동강에서 채집한 붕어와 양식 잉어에서는 간흡충 피낭유충이 전혀 검출되지 않았다. 각 실험어의 신선혈청을 이용하여 간흡충 탈낭유충에 대한 살충능을 조사한 결과 간흡충 피낭유충에 중감염된 참붕어 혈청과 붕어의 혈청은 잉어의 혈청에 비해 높은 살충능을 나타낸 반면, 간흡충 피낭유충에 감염되지 않은 참붕어의 혈청은 가장 낮은 살충능을 나타냈다. 각 어종의 혈청을 $47^{\circ}C$에서 30분간 처리하여 보체를 불활화시킨 결과 모든 실험어종에서 혈청의 살충능이 완전히 사라졌다. 간흡충 탈낭유충 균질액의 상층액을 각 어종의 두신 식세포에 첨가하여 chemiluminescent(CL) 반응을 측정한 결과 참붕어와 잉어에서 CL반응이 zymosan만을 첨가했을때에 비해 유의적(P<0.05)으로 감소하였으며, 각 어종별 간흡충 탈낭유충 균질액의 상충액 첨가에 의한 CL반응 감소율은 참붕어의 경우 22.9%, 붕어 9.6%,잉어 12.4%로 나타났다.

• Fisheries and Aquatic Sciences
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• 제3권2호
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• pp.78-82
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• 2000

The influence of daily water temperature changes on chemiluminescent (CL) response of phagocytes, plasma glucose level and mortality in cultured gray mullet (Mugil cephalus) was investigated. Among three experimental temperature groups, the fish in group I and group II were maintained constantly at $27^{\circ}C$ and $29^{\circ}C$, respectively, and those in group III were suffered daily elevation of water temperature from $27^{\circ}C$ to $29^{\circ}C$ for 5 hours, then restored again to $27^{\circ}C$. After 1 week of maintaining at each experimental tank, the fish in group III showed significantly lower CL peak value (p < 0.01) and higher plasma glucose levels (p < 0.05) than those in group I and group II. The cumulative mortality of group III was $86.9\%$ within 3 days after being subjected to acute handling stress. On the other hand, the cumulative mortalities of group I and group II were $12.5\%$ and $19.2\%$, respectively. In conclusion, gray mullet farms, especially, in the vicinity of thermoelectric power plants should avoid stressing the fish during periods of high water temperature.

• Fisheries and Aquatic Sciences
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• 제1권1호
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• pp.42-47
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• 1998

The chemiluminescent (CL) response of phagocytes from juvenile rockfish, Sebastes schlegeli, which were administered orally with levamisole was investigated. The fish intubated with doses of levamisole either at 0.5mg $kg^-$ or 1 mg $kg^-$body weight showed significant increase in CL responses at two weeks after the administration. The increased extent of CL in the fish exposed to 0.5 mg $kg^-$ body weight was considerably lower than that in the fish exposed to 1 mg $kg^-$. The fish exposed to 5 mg $kg^-$ body weight showed a steady and significant increase of CL response after the intubation. The fish intubated with 10 mg of levamisole $kg^-$ body weight, however, showed no significant differences in CL response after the administration. In the experiment of feeding experimental diet, a lower dose of levamisole induced immunostimulation of phagocytes, but higher doses of levamisole induced immunosuppression of phagocytes. At one week after marking and blood sampling, plasma glucose level was significantly increased in the control group and the group intubated 0.5 mg levamisole/kg body weight. However, the fish in another groups, which were administered higher levels of levamisole, showed no significant difference in glucose level after marking and blood sampling. The result of the present study suggests that levamisole can be used as a potent immunostimulator in rockfish by oral administration, and the immunomodulating activity of levamisole depends on the dosage used.

• 한국어병학회지
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• 제14권2호
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• pp.103-105
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• 2001

The effect of opsonization of zymosan with serum on the repiratory burst of haemocytes isolated from the Pacific oyster Crassostrea gigas was investigated. The reactive oxygen species (ROS) produced by the respiratory burst of haemocytes in response to the opsonized or unopsonized zymosan were measured using chemiluminescence (CL). The CL values were increased according to the increment of haemocyte number. The degree of CL amplification by increase of haemocytes from $0.1{\times}10^6$ to $0.5{\times}10^6$ cells/ml was 3-5 times, but comparatively low amplification was elicited by increase of haemocytes from $0.5{\times}10^6$ to $1{\times}10^6$ cells/ml. Chemiluminescences produced by the haemocytes in response to the zymosan opsonized with oyster serum were considerably higher than the CL produced by the haemocytes in response to the unopsonized zymosan.

• Fisheries and Aquatic Sciences
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• 제5권4호
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• pp.258-262
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• 2002

Effects of Escherichia coli genomic DNA on the production of phagocyte activating supernatants by the head kidney leucocytes isolated from olive flounder (Paralichthys olivaceus) were investigated. Phagocyte activating activity of the supernatants was estimated by. measuring reactive oxygen species (ROS) production in target head kidney phagocytes. All supernatants from olive flounder head kidney leucocytes-stimulated with E. coli DNA induced significantly (P<0.01) higher ROS production from target pagocytes than the unstimulated control supernatant. Maximum enhancement of chemiluminescent response was observed $5.0-10.0{\mu}g\;mL^{-1}$ of bacterial DNA while the increment ability was decreased significantly (P<0.01) at the concentration of $20.0{\mu}mL^{-1}$. The results demonstrate that olive flounder head-kidney leucocytes stimulated with bacterial DNA release a soluble phagocyte activating cytokines capable of enhancing the respiratory burst activity from target phagocytes.

• 한국어병학회지
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• 제20권2호
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• pp.189-200
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• 2007

잉어의 선천성 면역 인자가 관여하는 항바이러스 면역 반응을 조사하기 위해 UV-inactivated SVCV, Poly I:C 및 Con A를 주사한 후 3일째 라이소자임 활성, 혈청 내 보체의 살균능력 및 식세포의 활성산소량을 조사하였다. 그 결과, 모든 시험구에서 혈청 내 라이소자임의 활성은 유의적인 차이를 나타나지 않았으나, 두신 조직의 라이소자임 활성은 대조구에 비해 유의적으로 증가하였다. 또한, 혈청 내 보체의 살균 능력도 모든 시험구에서 대조구와 유의적인 차이가 없었다. 그러나 식세포의 활성은 UV-inactivated SVCV 시험구에서는 농도에 따라 증가한 것으로 나타났으며, Poly I:C 및 Con A 시험구에서는 저농도에서 활성이 증가한 것으로 나타났다. 바이러스에 대한 방어력을 조사하기 위해 주사 후 4일째 1×104 TCID50/fish 농도의 SVCV로 인위 감염한 결과 UV-inactivated SVCV 및 Poly I:C 시험구에서는 Con A 시험구에 비해 높은 방어력을 나타내었다. 또한, Poly I:C 시험구에서 라이소자임 및 식세포 활성이 다소 감소한 고농도에서도 높은 방어력이 유도된 것으로 나타나 이러한 결과는 Poly I:C에 의해 자극된 또 다른 비특이적 면역 인자가 SVCV에 대한 방어반응에 관여한 것으로 추정된다.

• 대한의생명과학회지
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• 제5권1호
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• pp.11-15
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• 1999

갑상선 질환의 진단에 필요한 갑상선 호르몬의 측정시 thyroxine (T$_4$)농도 측정방법이 가장 흔히 이용되고 있다. 과거에 많이 활용되어온 방사면역측정법 (RH)이 폐기물 처리 및 관리인원 등의 인적, 물적인 문제가 크게 대두되고 있는 점을 감안하면 효소결합면역측정법의 확립은 큰 의미가 있다고 하겠다. 본 연구에서는 thyroxine (T$_4$)에 대한 단클론 항체 (monoclonal antibody)를 이용하여 T$_4$에 horseradish-peroxidase를 화학적으로 결합시킨 microtiter plate에 HRP-conjugate T$_4$와 혈청내의 T$_4$간의 서로 경쟁적인 반응을 이용한 표준곡선을 얻었다. 그리고 이렇게 확립한 기법을 임상적인 갑상선질환 환자의 혈청을 기존의 chemiluminescence 방법을 이용하여 얻은 결과와 비교하여 본 실험에서 확립한 ELISA검사 수치의 유용성을 검토하였으며 이 방법의 임상이용 가능성을 검토하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권10호
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• pp.1478-1486
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• 2014

The present experiment was conducted to evaluate the efficacy of dietary fermented by-product of mushroom, Pleurotus ostreatus, (FBPM) as a fish meal (FM) replacer in juvenile Amur catfish, Silurus asotus. A total number of 225 fish averaging $5.7{\pm}0.1g$ ($mean{\pm}standard$ deviation) were fed one of the five experimental diets formulated to replace FM with FBPM at 0%, 5%, 10%, 20%, and 30% ($FBPM_0$, $FBPM_5$, $FBPM_{10}$, $FBPM_{20}$, and $FBPM_{30}$, respectively). At the end of eight weeks of the experiment, average weight gain (WG) of fish fed $FBPM_0$ or $FBPM_5$ were significantly higher than those of fish fed $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). However, there was no significant differences in WG among the fish fed $FBPM_0$, $FBPM_5$ or $FBPM_{10}$, and between fish fed $FBPM_{10}$ or $FBPM_{20}$, and also between those fed $FBPM_{20}$ or $FBPM_{30}$ diets. Lysozyme activity of fish fed $FBPM_0$ or $FBPM_5$ were significantly higher than those of fish fed $FBPM_{10}$, $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). The chemiluminescent response of fish fed $FBPM_5$ was significantly higher than those of fish fed $FBPM_0$, $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). Broken line regression analysis of WG suggested that the maximal dietary inclusion level for FBPM as a FM replacer could be 6.3% without any adverse effects on whole body composition and on serological characteristics. Therefore, these results may indicate that the maximal dietary inclusion level of FBPM as a FM replacer could be 6.3% in juvenile Amur catfish.