• Title/Summary/Keyword: chemical testing

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Effects of Pre-Sintering Surface Treatment and Liner Application on the Shear Bond Strength of Zirconia and Pressable Ceramic (소결 전 지르코니아 표면처리와 라이너 사용에 따른 지르코니아와 열가압성형도재의 전단결합강도)

  • Lee, Gwang-Young;Cho, Mi-Hyang;Choi, Sung-Min
    • Journal of Technologic Dentistry
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    • v.37 no.3
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    • pp.121-127
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    • 2015
  • Purpose: This study was intended to investigate the effect of applying liner for chemical bonding and physical surface roughness created on zirconia by using a sandpaper before sintering on the bond strength between the two materials. Methods: Zirconia blocks were cut using a low-speed cutter. Plate-shaped specimen($6mm{\times}6mm{\times}3mm$) was fabricated by sintering after giving surface roughness according to four kinds of sandpapers. Depending on whether or not to use liner, 60 specimens were divided into two groups ZN(non-liner), ZL(liner), and the two groups were subdivided into four groups respectively in accordance with sandpaper used, totaling eight groups (n=10). The surface roughness (Ra) values and shapes before sintering were observed, and shear bond strength after pressing ceramic plasticity was measured with a universal testing machine. For a test of the significance, a one-way ANOVA was performed, and Tukey's multiple comparison test was conducted. Results: The observation of the surface roughness was SB04($2.22{\pm}1.16{\mu}m$), SB08($2.98{\pm}0.33{\mu}m$), SB12($2.44{\pm}1.32{\mu}m$), SB20($2.34{\pm}0.59{\mu}m$) and SA04($2.34{\pm}0.67{\mu}m$), SA08($1.28{\pm}0.90{\mu}m$), SA12($2.03{\pm}1.60{\mu}m$), SA20($2.19{\pm}1.73{\mu}m$). In the case of ZN Group, the shear bond strength was ZN04($23.26{\pm}3.83MPa$), ZN08($21.76{\pm}2.33MPa$), ZN12($20.49{\pm}3.01MPa$), ZN20($24.98{\pm}4.22MPa$)(p<0.05). As for ZL Group, the shear bond strength was ZL04($25.09{\pm}5.67MPa$), ZL08($22.98{\pm}2.26MPa$), ZL12($21.54{\pm}5.70MPa$), ZL20($23.98{\pm}3.23MPa$)(p<0.05). Conclusion: The research results showed that the bond strength of Zirconia core and Pressing ceramic was further improved by physical surface treatment before sintering, rather than by chemical bonding through liner surface treatment.

3-D Analysis of Semiconductor Surface by Using Photoacoustic Microscopy (광음향 현미경법을 이용한 반도체 표면의 3차원적 구조 분석)

  • Lee, Eung-Joo;Choi, Ok-Lim;Lim, Jong-Tae;Kim, Ji-Woong;Choi, Joong-Gill
    • Journal of the Korean Chemical Society
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    • v.48 no.6
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    • pp.553-560
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    • 2004
  • In this experiment, a three dimensional structure analysis was carried out to examine the surface defects of semiconductor made artificially on known scale. It was investigated the three dimensional imaging according to the sample depth and the thermal diffusivity as well as the carrier transport properties. The thermal diffusivity measurement of the intrinsic GaAs semiconductor was also analyzed by the difference of frequency-dependence photoacoustic signals from the sample surface of different conditions. Thermal properties such as thermal diffusion length or thermal diffusivity of the Si wafer with and without defects on the surface were obtained by interpreting the frequency dependence of the PA signals. As a result, the photoacoustic signal is found to have the dependency on the shape and depth of the defects so that their structure of the defects can be analyzed. This method demonstrates the possibility of the application to the detection of the defects, cracks, and shortage of circuits on surface or sub-surface of the semiconductors and ceramic materials as a nondestructive testing(NDT) and a nondestructive evaluation(NDE) technique.

Immuno-chromatographic Analysis for HPV-16 and 18 E7 Proteins as a Biomarker of Cervical Cancer Caused by Human Papillomavirus

  • Kim, Joo-Ho;Cho, Il-Hoon;Seo, Sung-Min;Kim, Ji-Sook;Oh, Kyu-Ha;Kang, Heun-Soo;Kim, In-Gyu;Paek, Se-Hwan
    • Bulletin of the Korean Chemical Society
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    • v.30 no.12
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    • pp.2999-3005
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    • 2009
  • Among the more than 120 different types of human papillomavirus (HPV), types 16 and 18 have been known to be high risk agents that cause cervical cancer. We examined, in an immuno-chromatographic analysis, the potential of using the early gene product, E7 protein, as a diagnostic marker of cervical cancer caused by HPV. We developed monoclonal antibodies specific to HPV-16 and 18 E7 proteins that were produced from bacterial cells using gene recombinant technology. For each E7 protein, the optimal antibody pair was selected using the immuno-chromatographic sandwichtype binding system based on the lateral flow through membrane pores. Under these conditions, this rapid testing assay had a detection capability as low as 2 ng/mL of E7 protein. Furthermore, since viral analysis required the host cell to be lysed using chemicals such as detergents, it was possible that the E7 protein was structurally damaged during this process, which would result in a decrease in detection sensitivity. Therefore, we examined the detrimental effects caused by different detergents on the E7 protein using HeLa cells as the host. In these experiments, we found that the damage caused by the detergent, nonylphenylpolyethylene glycol (NP-40), was minimal relative to Triton X-100 commonly used for the cell lysis. Temperature also affected the stability of the E7 protein, and we found that the E7 protein was stabilized at 4$^{\circ}C$ for about 2 h, which was 4 times longer than at room temperature. Finally, a HPV-infected cervical cancer cell line, which was used as a real sample model, was treated using the optimized conditions and the presence of E7 proteins were analyzed by immuno-chromatography. The results of this experiment demonstrated that this rapid test could specifically detect HPV-infected samples.

Fermentative characteristics of wheat bran direct-fed microbes inoculated with starter culture

  • Kim, Jo Eun;Kim, Ki Hyun;Kim, Kwang-Sik;Kim, Young Hwa;Kim, Dong Woon;Park, Jun-Cheol;Kim, Sam-Chul;Seol, Kuk-Hwan
    • Korean Journal of Agricultural Science
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    • v.43 no.3
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    • pp.387-393
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    • 2016
  • This study was conducted to determine the fermentative characteristics of wheat bran inoculated with a starter culture of direct-fed microbes as a microbial wheat bran (DMWB) feed additive. Wheat bran was prepared with 1% (w/w, 0.5% Lactobacillus plantarum and 0.5% of Saccharomyces cerevisiae) starter culture treatment (TW) or without starter culture as a control (CW). Those were fermented under anaerobic conditions at $30^{\circ}C$ incubation for 3 days. Samples were taken at 0, 1, 2, and 3 days to analyze chemical composition, microbial growth, pH, and organic acid content. Chemical composition was not significantly different between CW and TW (p > 0.05). In TW, the number of lactic acid bacteria and yeast increased during the 3 days of fermentation (p < 0.05) and the population of lactic acid bacteria was significantly higher than in CW (p < 0.05). After 3 days, the number of yeast in TW was $7.50{\pm}0.07log\;CFU/g$, however, no yeast was detected in CW (p < 0.05). The pH values of both wheat bran samples decreased during the 3 days of fermentation (p < 0.05), and TW showed significantly lower pH than CW after 3 days of fermentation (p < 0.05). Contents of lactic acid and acetic acid increased significantly at 3rd day of fermentation in TW. However, no organic acids were generated in CW during testing period. These results suggest that 3 days of fermentation at $37^{\circ}C$ incubation after the inoculation wheat bran with starter culture makes it possible to produce a direct-feed with a high population of lactic acid bacteria at more than $10^{11}CFU/g$.

Strength Characteristics on Sulfuric Acid Corrosion of Recycled PET Polymer Concrete with Different Fillers (충전재 종류에 따른 PET재활용 폴리머콘크리트의 황산부식에 대한 강도 특성)

  • Jo Byung-Wan;Shin Kyung-Chul;Park Seung-Kook
    • Journal of the Korea Concrete Institute
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    • v.17 no.4 s.88
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    • pp.499-504
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    • 2005
  • Polymer concrete shows excellent mechanical properties and chemical resistance compared with conventional normal cement concrete. The polymer concrete Is drawing a strong interest as high-performance materials in the construction industry Resins using recycled PET offer the possibility of a lower source cost of materials for making useful polymer concrete products. Also the recycling of PET in polymer concrete would help solve some of the solid waste problems Posed by plastics and save energy. An objective of this paper is to estimate the damage of sulfuric acid, through investigating recycled PET polymer concrete, immersed at sulfuric acid solution for 84 days. As a result of testing, recycled PET PC, used $CaCO_3$ as filler, makes a problem of appearance and strength if they are exposed for long term at corrosion environment. On the other hand, recycled PET PC, used fly-ash as filler, had less effect on decrease in weight and strength. Recycled PET PC is excellent chemical resistance, resulting in the role of unsaturated polyester resin which consists of polymer chain structure accomplishes bond of aggregates and filler strongly. Also, recycled PET PC, used fly-ash as filler, is stronger resistance of sulfuric acid corrosion than $CaCO_3$, because it is composed of $SiO_2$ and very strong glassy crystal structure. Therefore, recycled PET PC, used fly-ash as filler, is available under corrosion circumstances like sewer pipe or waste disposal plant.

Development of Battery-free SAW Integrated Microsensor for Real Time Simultaneous Measurement of Humidity and $CO_2$ component (습도와 $CO_2$ 농도의 실시간 동시감지를 위한 무전원 SAW 기반 집적 센서 개발)

  • Lim, Chun-Bae;Lee, Kee-Keun;Wang, Wen;Yang, Sang-Sik
    • Journal of the Microelectronics and Packaging Society
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    • v.16 no.1
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    • pp.13-19
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    • 2009
  • A 440MHz wireless and passive surface acoustic wave (SAW) based chemical sensor was developed on a $41^{\circ}YX\;LiNbO_3$ piezoelectric substrate for simultaneous measurement of $CO_2$ gas and relative humidity (RH) using a reflective delay line pattern as the sensor element. The reflective delay line is composed of an interdigital transducer (IDT) and several shorted grating reflectors. A Teflon AF 2400 and a hydrophilic $SiO_2$ layer were used as $CO_2$ and water vapor sensitive films. The coupling of mode (COM) modeling was conducted to determine optimal device parameters prior to fabrication. According to simulation results, the device was fabricated and then wirelessly measured using the network analyzer. The measured reflective coefficient $S_{11}$ in the time domain showed high signal/noise (S/N) ratio, small signal attenuation, and few spurious peaks. In the $CO_2$ and humidity testing, high sensitivity ($2^{\circ}/ppm$ for $CO_2$ detection and $7.45^{\circ}/%$RH for humidity sensing), good linearity and repeatability were observed in the $CO_2$ concentration ranges of $75{\sim}375ppm$ and humidity levels of $20{\sim}80%$RH. Temperature and humidity compensations were also investigated during the sensitivity evaluation process.

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A Study on Development of Eco-friendly Wrap using Biodegradable Resin (생분해성 수지를 이용한 환경친화성 랩 개발에 관한 연구)

  • Lim, Mi-Jin;Sim, Jae-Ho;Choi, Jong-Moon;Kim, Young-Sik
    • Applied Chemistry for Engineering
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    • v.16 no.6
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    • pp.800-808
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    • 2005
  • The compounding resin for biodegradable wrap was developed, and its manufacturing process and physical properties were studied. For these purposes, following factors were optimized: the types and amounts of raw resin material, anti-oxidants, and lubricants used. In this work, the stable compounding resin used to make biodegradable wrap was based on poly(butylene adipate-co-butylenesuccinate) (PBAS) and poly(butyleneadipate-co-butylene succinate-co-butyleneterephthalate) (PBAST). The improved properties of resin with an additive were investigated by melting flow index (MFI). From these results, the physical properties of compounding resin, based on PBAST, were more than those of PBAS. For PBAS, the Irganox 1010, 1076 and Irgafos TNPP as the first and second anti-oxidants, respectively, were good. For PBAST, the good first and second anti-oxidants, respectively, were Irganox 1076 and Mark PEP 36. The good lubricants for feeding PBAS and PBAST were glycerol monostearate and palmityl alcohol, respectively. The stability and tensile strength experiment of wrap were also investigated by the elution of heavy metals and universal testing machine (UTM), respectively. The decomposition ratio of developed wrap was increased proportional to the reclaiming time. The degradation ratio of compounding resin sample was about 60% after 40 days.

Effects of Granular Silicate on Watermelon (Citrullus lanatus var. lanatus) Growth, Yield, and Characteristics of Soil Under Greenhouse

  • Kim, Young-Sang;Kang, Hyo-Jung;Kim, Tae-Il;Jeong, Taek-Gu;Han, Jong-Woo;Kim, Ik-Jei;Nam, Sang-Young;Kim, Ki-In
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.5
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    • pp.456-463
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    • 2015
  • The objective of this study was to determine the effects of granular type of silicate fertilizer on watermelon growth, yield, and characteristics of soil in the greenhouse. Four different levels of silicate fertilizer, 0(control), 600, 1,200, $1,800kg\;ha^{-1}$ were applied for experiment. The silicate fertilizer was applied as a basal fertilization before transplanting watermelon. Compost and basal fertilizers were applied based on the standard fertilizer recommendation rate with soil testing. All of the recommended $P_2O_5$ and 50% of N and $K_2O$ were applied as a basal fertilization. The N and $K_2O$ as additional fertilization was split-applied twice by fertigation method. Watermelon (Citrullus lanatus Thunb.) cultivar was 'Sam-Bok-KKuol and main stem was from rootstock (bottle gourd: Lagenaria leucantha Standl.) 'Bul-Ro-Jang-Sang'. The watermelon was transplanted on April, 15. Soil chemical properties, such as soil pH, EC, available phosphate and exchangeable K, Mg, and available $SiO_2$ levels increased compared to the control, while EC was similar and the concentrations of soil organic matter decreased. Physical properties of soils, such as soil bulk density and porosity were not different among treatments. The growth characteristics of watermelon, such as stem diameter, fresh and dry weight of watermelon at harvest were thicker and heavier for silicate treatment than the control, while number of node was shorter than the control. Merchantable watermelon increased by 3-5% compared to the control and sugar content was 0.4 to $0.7^{\circ}Brix$ higher than the control. These results suggest that silicate fertilizer application in the greenhouse can improve some chemical properties of soils and watermelon stem diameter and dry weight, which are contributed to watermelon quality and marketable watermelon production.

Vulnerability Assessment for Fine Particulate Matter (PM2.5) in the Schools of the Seoul Metropolitan Area, Korea: Part I - Predicting Daily PM2.5 Concentrations (인공지능을 이용한 수도권 학교 미세먼지 취약성 평가: Part I - 미세먼지 예측 모델링)

  • Son, Sanghun;Kim, Jinsoo
    • Korean Journal of Remote Sensing
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    • v.37 no.6_2
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    • pp.1881-1890
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    • 2021
  • Particulate matter (PM) affects the human, ecosystems, and weather. Motorized vehicles and combustion generate fine particulate matter (PM2.5), which can contain toxic substances and, therefore, requires systematic management. Consequently, it is important to monitor and predict PM2.5 concentrations, especially in large cities with dense populations and infrastructures. This study aimed to predict PM2.5 concentrations in large cities using meteorological and chemical variables as well as satellite-based aerosol optical depth. For PM2.5 concentrations prediction, a random forest (RF) model showing excellent performance in PM concentrations prediction among machine learning models was selected. Based on the performance indicators R2, RMSE, MAE, and MAPE with training accuracies of 0.97, 3.09, 2.18, and 13.31 and testing accuracies of 0.82, 6.03, 4.36, and 25.79 for R2, RMSE, MAE, and MAPE, respectively. The variables used in this study showed high correlation to PM2.5 concentrations. Therefore, we conclude that these variables can be used in a random forest model to generate reliable PM2.5 concentrations predictions, which can then be used to assess the vulnerability of schools to PM2.5.

Development of COVID-19 Neutralizing Antibody (NAb) Detection Kits Using the S1 RBD Protein of SARS-CoV-2 (코로나 바이러스 감염증-19의 재조합 S1 RBD 단백질을 이용한 COVID-19 바이러스의 중화항체 검사 키트의 개발)

  • Choi, Dong Ok;Lee, Kang Moon
    • Korean Journal of Clinical Laboratory Science
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    • v.53 no.3
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    • pp.257-265
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    • 2021
  • The COVID-19 virus is a β-genus virus that causes infection by mediating the angiotensin convertible enzyme 2 (ACE2) receptor, which is distributed in large numbers in the human respiratory tract. The disease requires effective post-management of antibody production by complete healers and vaccinators because there is no perfect remedy for the virus infection. This study aimed to develop recombinant proteins specifically responsive to neutralizing antibodies in clinical specimens and use them to develop a rapid diagnostic kit to diagnose neutralizing antibodies quickly and conveniently against the COVID-19 virus and confirm the possibility of commercialization through a performance evaluation. Rapid diagnostic kits using COVID-19 S1 RBD recombinant proteins can be applied to rapid diagnostic kits, with positive percentage agreement (PPA) and negative percentage agreement (NPA) of 100% and 98.3%, respectively, compared to the U.S. FDA-approved ELISA kits. If the performance of the rapid diagnostic kit is improved and neutralizing antibodies can be analyzed quantitatively using quantitative analysis equipment, it can be used as important data to predict immunity to the COVID-19 virus and determine additional vaccinations.