• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.6
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• pp.663-666
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• 2009

Anti-proliferation effects of Corni fructus ethanol extracts were investigated in the RC58T/h/SA#4 cells treated with environmental hormones including dioxin and bisphenol A. The proliferation was decreased at the concentration over $500{\mu}g/mL$ in the RC58T/h/SA#4 cells with ethanol extracts of various concentrations (1, 10, 100, 500, and $1000{\mu}g/mL$). The environmental hormones such as dioxin and bisphenol A increased the growth of RC58T/h/SA#4 cells in the charcoal-treated FBS (cFBS) medium. The proliferation was the highest at 1 nM and $0.1{\mu}M$ for the tested dioxin and bisphenol A concentration, respectively. Ethanol extracts showed inhibition of the proliferation in a dose-dependent manner at the tested concentrations (10, 100, 300, and $500{\mu}g/mL$) in the RC58T/h/SA#4 cells treated with the environmental hormones. The anti-proliferation was the highest at $500{\mu}g/mL$ concentration among the tested ethanol extracts.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.19-23
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• 2002

This experiment was conducted to micropropagate bulblets via shoot cluster formation and massproduce normal bulblets from the sections of proliferated shoot clusters in Lilium asiatic hybrid 'Hae Hwa'. The induction of shoot clusters from the culture of bulblet sections was more effective than that of bulb scales on MS medium with 1.0 mg/L BA and 0.5 mg/L IAA. Proliferation of shoot clusters from the formed shoot cluster sections was the most favorable on medium containing 5.0 mg/L BA and 0.5 mg/L IAA. The formation and the growth of bulblets from shoot cluster sections were achieved effectively on medium with 60∼90 g/L sucrose. The leaves derived from shoot clusters grew vigorously but the bulblets from shoot clusters grew very poor in 5L air-lift bioreactor culture. By the addition of 30 mL fresh liquid medium containing doulble strength MS salts, 250 g/L sucrose and 5 g/L activated charcoal after 8 weeks in the shoot cluster culture on MS medium with 5.0 mg/L BA and 0.5 mg/L IAA, the number of bulblets was increased in light condition, but the growth of bulblets was not affected by light. Bulblet production was possible with the bulblet product at 53 to 68 mg in fresh weight by liquid medium addition after the proliferation of shoot cluster.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.219-223
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• 1998

Anthers contanining uninucleate microspores of eight cultivars of pepper were cultured on MS medium supplemented with 0.004mg/L 2,4-D and 0.1mg/L kinetin, 3% sucrose and 0.2% Gelrite, kept at $35^{\circ}C$ for 24 h, and then cultured at $25^{\circ}C$ with a photoperiod of 16 h daylight for 40 days. Frequency of embryoid and callus formation was varied with cultivars. Embryoid formation was found in Cheongyang and Fushimi Amanaga, while callus formation was in California Wonder, Fushimi Amanaga and Geoseong. In anther culture medium supplemented with 1% activated charcoal, embryoid formation was found with 0.5% of frequency only in Cheongyang, while no callus formation was found. In 1/2MS medium, frequency of embryoid formation in Shishitou, Yatsufusa and Taka no Tsume was 1.2%, 0.4% and 0.4%, respectively. On the other hand, in 1/2 B5 medium, frequency of callus formation in Yatsufusa and Taka no Tsume was 2.8% and 2.7%, respectively. Embryoids transferred to hormone-free MS medium were developed to plantlets and acclimatized. The number of chromosomes in the root tip cells of the haploid plant was 2n=$\times$=12 in cv. Cheongyang.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.53-53
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• 2019

Cypripediums, popularly called lady's slippers or moccasin flowers, are the showiest and most sought after hardy terrestrial orchids, collected and grown by orchid and alpine plant enthusiasts alike. In Korea, 4 species of cypripedium are reported as Cypripedium japonicum, C. macranthos, C. guttatum, and C. calceolus. We had already reported the feasibilities of C. macranthos and C. guttatum with in vitro germination methods from immature seeds. The seeds of white lady's slipper orchid (Cypripedium macranthos Sw. alba) were collected 65 days after pollination in 2018. The green pods were sterilized with flame and sowed immediately on the POM(Phytomax orchid maintenance media(R), Sigma) supplemented with BAP 0, 0.5, 1.0 mg/L and NAA 0, 1, 2mg/L. The germination of seed was observed 90 days after sowing, and the plantlets were subcultured to the same media according to the size of the protocorm with 1~2, 2~3, 3~4, 5~6, 7~8mm. The time of the subculture to the new media seems to be critical factors of forming rhizoids which is the hairy root of the cypripediums. As a results, the protorms of the white lady's slipper orchid was successfully germinated in the POM media supplemented BAP 0.5 and NAA 1.0 mg/L. The roots and rhizoids were formed in 5~6mm protocorms subculture over 95% survival ratio. We also tried to subculture to liquid medium without activated charcoal, however the browning or malformation of the roots was observed in the root. The formation of shoots from the protocorm was effectively enhanced in the POM media with non-additives of plant growth regulators. These results indicate the possibility of high and stable production and practical industrialization of endangered white lady's slipper orchids.

• Journal of the Korean GEO-environmental Society
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• v.20 no.1
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• pp.5-11
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• 2019

The main objective of this study was to analyse polycyclic aromatic hydrocarbons in surface sediments obtained from near Nakdong estuary, and to estimate ecological risks of PAHs using PAHs concentrations. The main constituents of sediments were $SiO_2$, $Al_2O_3$, CaO, $Fe_2O_3$, and ignition loss of sediments ranged from 2.97% and 8.39%. Total concentrations of PAHs ranged from $128.4ng\;g^{-1}$ and $507.4ng\;g^{-1}$, and the major PAHs were 2 ring and 4 ring aromatic hydrocarbons. Each concentrations of PAHs are all below effect range low, which indicated that each PAHs in 8 studying sites show low ecological risk. From M-ERM-Q analyses, M-ERM-Q values of 8 studying sites are below 0.1 indicating low ecological risk. From source apportionment analyses, PAHs come from grass, wood, charcoal combustion for N-1 and N-7, petroleum combustion for N-5 and N-6, petroleum pollution for N-2, N-3, N-4, N-8.

• Journal of the Korean Society of Floral Art and Design
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• no.42
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• pp.145-156
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• 2020

The purpose of this study was to investigate status survey on flower products with high sales by item and peak season. The survey was implemented for 103 people who are running flower shop and are members of association for Engineer Floral Design who are acquired Craftsman Floral Design and Engineer Floral Design. The result of this study, the peak season for the sale of flower product is showed May (52.0%) and February (24.3%). The reason for the selection was in May, month of family, teacher's day, etc. in February, personnel transfer and graduation ceremony. As a result of researching flower products with high sales volume by category, Cut flowers were ranked the first place of bouquet (51.5%%, 40.3%) and the second place of basket (28.8%, 37.5%) on annual and peak season. And also, Corsage, which was 6^th (1.8%) in the annual sales volume survey, increased to 3^rd (8.0%) during peak season. Pot plants were ranked 1st of foliage plant (50.6%, 51.3%) and 2nd tropical orchid·oriental orchids (27.4%, 28.3%) on annual and peak season. And also, Charcoal and bonsai, which were unresponsive in the annual sales survey, increased sales volume to 0.7% and 2.6%, respectively, during peak season. Processed flowers were highest ranked dry flower (45.3%, 39.5%), preserved flower (27.7%, 37.2%) on both non peak season and peak season. And then followed by artificial flower, pressed flower and collage.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.27 no.3
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• pp.144-157
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• 2022

Noble gases, which are chemically inert and behave conservatively in marine environments, have been used as tracers of physical processes such as air-sea gas exchange, mixing of water masses, and distribution of glacial meltwater in the ocean. For precise measurements of Ne, Ar, and Kr, we developed a mass spectrometric system consisting of a quadrupole mass spectrometer (QMS), a high vacuum preparation line, an activated charcoal cryogenic trap (ACC), and a set of isotope standard gases. The high vacuum line consists of three sections: (1) a sample extraction section that extracts the dissolved gases in the sample and mixes them with the standard gases, (2) a gas preparation section that removes reactive gases using getters and separates the noble gases according to their evaporation points with the ACC, and (3) a gas analysis section that measures concentrations of each noble gas. The ACC attached to the gas preparation section markedly lowered the partial pressures of Ar and CO₂ in the QMS, which resulted in a reduced uncertainty of Ne isotope analysis. The isotope standard gases were prepared by mixing ²²Ne, ³⁶Ar, and ⁸⁶Kr. The amounts of each element in the mixed standard gases were determined by the reverse isotope dilution method with repeated measurements of the atmosphere. The analytical system achieved precisions for Ne, Ar, and Kr concentrations of 0.7%, 0.7%, and 0.4%, respectively. The accuracies confirmed by the analyses of air-equilibrated water were 0.5%, 1.0%, and 1.7% for Ne, Ar, and Kr, respectively.

• Korean Chemical Engineering Research
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• v.61 no.3
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• pp.472-478
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• 2023

In this study, the acetaldehyde removal characteristics of activated carbon (AC) for air purifier filters were investigated using metal catalysts-impregnation and functional group-modification method. The AC with a high specific surface area(1700 m²/g) and micropores was prepared by KOH activation of coconut charcoal and the efficiency of catalyst and functional group immobilization was examined by varying the drying conditions within the pores after immersion. The physical properties of the prepared activated carbon were analyzed by BET, ICP, EA, and FT-IR, and the acetaldehyde adsorption performances were investigated using gas chromatography (GC) at various impregnation and modified conditions. As the concentration of impregnation solution increased, the amount of impregnated metal catalysts increased, while the specific surface area showed a decreasing trend. The adsorption tests of the metal catalyst-impregnated and functional group-modified activated carbons revealed that excellent adsorption performance in compositions MgO10@AC, CaO10@AC, EU10@AC, and H-U3N1@AC, respectively. The MgO10@AC, which showed the highest adsorption performance, had a breakthrough time of 533.8 minutes and adsorption capacity of 57.4 mg/g for acetaldehyde adsorption. It was found that the nano-sized MgO catalyst on the activated carbon improved the adsorption performance by interacting with carbonyl groups of acetaldehyde.

• Korean Journal of Organic Agriculture
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• v.10 no.3
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• pp.67-77
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• 2002

This study was conducted to investigate an effects on ago-chemical alternative materials such as the wood vinegar, a lactic acid bacteria serum, the fermented plant juice, the brown rice vinegar and a Chitosan used for amount and qualities of fruits and to examine the pest protection efficiency for their uses in the apple and pear orchard farms. An apple yields in the orchard cultivated with using the ago-chemical alternative materials without appling the fertilizer and pesticides were decreased at 56% relative to the conventional farming practice method. Also, it was indicated that there was difficult to produce the fruits with marketability because the small sizes of fruits were produced. For the quality of fruits, the brix of apple produced in the orchard cultivated with using the ago-chemical alternative materials was similar, but Vitamin C content was greater than that of the conventional farming practice method. As a results of treating with the wood vinegar, a lactic acid bacteria serum, the fermented plant juice, the brown rice vinegar and a Chitosan instead of applying pesticides, the fruit disease in the Chitosan treatment was a little decreased, but was great occurred in the other treatments compared with the conventional farming practice mehod. However, it observed that brix and Vitamin C content of apple produced in the Chitosan, brown rice vinegar, fermented plan juice and fish amino acid treatments and in the Chitosan, brown rice vinegar, charcoal power and peat moss treatments were greater than those of the conventional farming practice method, respectively. Over all, it considered that there was very difficult to manage the orchard depended on the ago-chemical alternative materials without appling the chemical fertilizer and pesticides in the apple orchard, but it might be proper to use the ago-chemical alternative materials as an auxiliary means to decrease the appling amount of chemical fertilizer and pesticides. Furthermore, the general effects on the ago-chemical alternative materials to the perennial fruits should be investigated with considering the changes of soil fertility, soil microbial status and natural enemy creatures after treating them for a long time.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.31 no.3
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• pp.61-74
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• 2013

This study examined and analyzed ancient writing and poetry regarding cases of appreciating plants by using pots in a garden of a palace or private houses by ancestors, and examined shape and planting method of plant species and potted plants, arrangement and preference of potted plants. As for the method of the study, description research method which examines and interprets poem and painting based on potted plants. The results of this study is summarized like the following. First, the plants which were favorably used for potted plants include 19 kinds such as Prunus mume, Pinus densiflora, Pinus pumila, Phyllostachys spp., Camellia japonica, Punica granatum, and Gardenia jasminoidesa, and as for herbs, 12 kinds such as Chrysanthemum monifolium and Nelumbo nusifera . Second, the species which were specially arranged into artificial shapes include Prunus mume and Pinus densiflora. The two plants made the shape of severe curves of stems such as Wangpi. Gyuban, and Bangan, and there are Pinus densiflora dwarfed potted plant whose roots are stretched on Prunus mume grafted into a strange stump and an oddly shaped stone. For the beauty of the dwarfed tree shape, pine cones are added to an old Pinus densiflora or Parthenocissus tricuspidata is planted to stems, and additional method of making moss on the soil, which is called 'Jongbunchuigyeong'. As for planting method, water culture, planting on a stone, planting on a charcoal, and assembled planting are expressed in poetry. Third, as for external space for potted plants, a place where a king stays, a bed room for a king, surrounding areas and gardens of private houses, and step stones were used as a space which adds artistic effects. Potted plants are placed on a table in a library, on a desk, on a drawer, and near a pillow as a small items in a room, and scholars enjoyed original characteristics and symbolism of the potted plants. Fourth, at the time of flowering of Prunus mume, poetry event was held to enjoy the tree and writing poetry begun. And at the time of flowering of Chrysanthemum monifolium, the flowers were floated in a liquor glass or shadow play was enjoyed. Fifth, potted plants played the role of garden ornaments in elegant events of a palace, the gentry, wedding ceremony, and sacrificial rites. Sixth, potted plants were used as tributes between countries, donation to a king, or a gift of a king. In addition, there were many cases where scholars exchanged potted plants and there is the first record of giving a potted plant in 'Mokeunsigo' by Mokeun Isaek, scholar in the late era of Goryeo. Seventh, at the time of flowering Prunus mume, Chrysanthemum monifolium, Gardenia jasminoides, Nelumbo nusifera, and Narcissustazetta var. chinensis, they enjoyed the particular fragrance and express it into poetry. Eighth, plant species from southern parts such as Camellia japonica, Daphne odora, Gardenia jasminoides, Citrus unshiu, Phyllostachys spp., Punica granatum, Rosa rugosa, and Musa basjoo, or foreign plant species, and species weak against the cold were utilized as pot plants for enjoying green trees indoors in northern central province in harshly cold winter.