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한국산 도꼬마리 추출물의 항균효과 및 분리 정제

  • 김현수;신재욱
    • Microbiology and Biotechnology Letters
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    • v.25 no.2
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    • pp.183-188
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    • 1997
  • Antimicrobial activity of various extracts of Xanthium strumarium L. was tested against 25 strains of bacteria, yeast and fungus. The crude ethylacetate extract exhibited strong growth inhibition to the tested strains with the exception of partial Gram-negative bacteria. The property of antimicrobial compound was very stable under heat treatment at $120^{\circ}C$, but it was unstable in acid (pH 3.0) and alkali (pH 10.0) treatment. The antimicrobial compounds were purified by boiling water extraction, ethylacetate extraction, charcoal column chromatography, silica gel column chro- matography and reverse phase HPLC. The purified compound A and B were detected in a single peak (each above 98% purity) through the HPLC analysis. The compound A and B showed a strong growth inhibition against Gram-negative and positive bacteria in the agar diffusion method. When tested by the FDA method using the esterase, compound A mainly inhibited the growth of bacteria and compound B showed the growth inhibition of both bacteria and yeasts.

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Adsorption Removal of Odor Compounds (IBMP, IPMP) (이.취미물질(IBMP, IPMP)의 흡착제거)

  • 김은호;손희정;김영웅
    • Journal of environmental and Sanitary engineering
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    • v.14 no.2
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    • pp.18-24
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    • 1999
  • The purpose of this study was carried out to estimate removal possibility of IBMP and IPMP causing odor in raw water. As a result of Freundlich isotherm. IBMP was superior to IPMP in adsorptive capacity. Adsorptive capacities of activated carbon were found to be in order of Lignite, Coconut shell, and Charcoal. These were well fitted with Freundlich isotherm. According to adsorption breakthrough tests for Lignite GAC, breakthrough time of IPMP and IBMP were 5.7hr and 5.5hr, respectively. Because adsorptive capacities of target material were very different with pore size distribution, it seemed that Lignite and Coconut shell based activated carbons were recommended in order to remove door compounds.

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Wood Identification of Neolithic Charcoals Excavated at Giheung Nongseori Ruins (기흥 농서리유적에서 발굴된 신석기시대 목탄의 목재 식별)

  • Eom, Young-Geun;Xu, Guang Zhu
    • Journal of the Korean Wood Science and Technology
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    • v.38 no.4
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    • pp.275-281
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    • 2010
  • Wood identification was conducted for the 8 Neolithic charcoals excavated at Giheung Nongseori Ruins through scanning electron microscopy. They were all identified as hardwood: 6 and 1 were found to be Prinus section and Cerris section of subgenus Lepidobalanus under genus Quercus, respectively, and the remaining 1 to be genus Betula. This species composition was thought to indicate the temperate to warm temperate climate region.

Local Knowledge on Trees Utilization and Their Existing Threats in Rashad District of Nuba Mountains, Sudan

  • Adam, Yahia Omar
    • Journal of Forest and Environmental Science
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    • v.30 no.4
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    • pp.342-350
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    • 2014
  • Rural people of Sudan are endowed with a deep knowledge concerning the utilization of different tree species. However research on the local knowledge related to tree species utilization still lacks adequate attention. The study objectives were to identify the existing local knowledge related to the utilization of the tree species and the existing threats to the availability of the trees. A total of 300 respondents were selected randomly from Rashad district in Nuba Mountains in 2011. Semi-structured interview, direct observation, group discussion, preference ranking and direct matrix ranking were used to collect the data. The study results revealed that people of Nuba Mountains utilize different tree species for food, medicinal purposes, fodder, firewood, construction and cultural ceremonies. The study results also indicated that the availability of trees is negatively influenced by firewood collection, agricultural expansion, drought, overgrazing and charcoal production. The study concluded that local knowledge has crucial role in tree species utilization in Nuba Mountains. Further researches to document and substantiate the local knowledge on useful tree species are highly recommended.

Performance Evaluation of Polymer Cementitious Interior Finish Using Light-Weight Aggregates Containing Pyroligneous Liquid (목초액 담지체를 골재로 사용한 폴리머 시멘트계 내장마감재의 성능평가)

  • Lee, Chae-Young;Kim, Joo-Young;Kim, Wan-Ki
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2014.05a
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    • pp.218-219
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    • 2014
  • The purpose of this study is to develop eco-friendly internal material of acrylic emulsion mortars using light-weight aggregate carrier which contains pyroligneous liquid. Four types of light-weight aggregates (vermiculite, perlite, charcoal, zeolite) that are widely used in building materials are selected and the properties such as adhesion, water absorption coefficient, antibiosis, crack and impact resistance are evaluated in accordance with KS F 4715. As a result, the properties of acrylic emulsion mortars using light-weight aggregate carrier are satisfied with KS requirements. The antibiosis is improved with increasing zeolite light-weight carrier content.

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Extraction of Impregnated Metals from Charcoal Active Carbon with Supercritical Carbon Dioxide (초임계 이산화탄소에 의한 금속 담지 활성탄으로부터 담지금속의 추출)

  • Lee, Jong-Chol;Ryu, Sam-Gon
    • Journal of the Korea Institute of Military Science and Technology
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    • v.11 no.5
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    • pp.99-106
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    • 2008
  • Metal ions such as Cu and Cr were extracted from the Cu, Cr and Ag impregnated active carbon by contacting the solid surfaces with supercritical carbon dioxide(Sc-$CO_2$) containing chelating agents. About 10g of the active carbon sample were loaded in a vertical tube extractor contacting with $CO_2$ flowing from the bottom of the tube for 6hrs. The ligands used were acetyl acetone(AA) and Cyanex-302(C-302). Water and methanol were used as entrainers to study the effect of co-solvent to $CO_2$. Experimental results showed that C-302 was more effective than AA in removing Cu with the maximum extraction of 42.0wt%, while 57.6wt% of Cr was extracted with AA from the sample.

High Efficiency of Plant Regeneration from Seed-Derived Callus of Zoysiagrass cv. Zenith (Zoysiagrass japonica의 효율적인 재분화체계에 관한 연구)

  • Ming Liang Chai
    • Asian Journal of Turfgrass Science
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    • v.12 no.4
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    • pp.195-202
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    • 1998
  • The development of a protocol for high efficiency of embryogenic callus separation, maintenance and plant regeneration from the seeds of zoysiagrass cv. Zenith was studied. Embryogenic callus ratio is absolutely determined by genotype, but by adding high concentration of copper into medium, changing light condition and maintaining callus on initial induction medium for 8∼10 weeks, embryogenic callus can be easily distinguished and its growth can be promoted. There were significant differences among selected callus lines (each from one seed) according to their growth rates and regeneration percentages. Callus pre-treatment with activated charcoal inhibited callus growth, increased the level of precocious germination during culture and promoted shoot cluster formation after transfer to regeneration medium. For long-term callus maintenance, N6AA medium was better than MS medium, because the former inhibited non-embryogenic callus formation and kept vigor of embryogenic callus. The best callus lines Z-(5) has been successfully used for transformation and somaclonal variation selection in our laboratory.

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Evaluation of Laver Growth Rate using Pyroligneous Acid (목초액유기산을 사용한 김의 성장률 평가)

  • Kim, U-Hang;Jo, Seong-Taek
    • Proceedings of KOSOMES biannual meeting
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    • 2004.11a
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    • pp.105-108
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    • 2004
  • Organic acid is made with carbonized organic acid that is produced from charcoal burning process. It is evaluated whether carbonized organic acid is able to removed Enteromorpha in the laboratory and Porphra aquaculture farm test. The optimum condition for Enteromorpha removal are revealed ten times dilution and ten second immersion. The mortality rate of Enteromorpha is $95\%$ and diatom-detaching rate is $100\%$ by the organic acid treatment. On the other hand, the mortality rate of Porphra is lower than $5%\$. It was measured that nitrogen was 0.175 mg/l and phosphorus was 0.0158 mg/l. Therefore, Concentration of nutrients were lower than being necessary to Porphra growth. Growth rate of Porphra was $12\%$ increased by organic acid treatment with carbonized organic acid added nutrient.

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Clonal Propagation through Leaf Sheath Culture of Phalaenopsis

  • Jo, Man-Hyun;Ham, In-Ki;Lee, Mi-Ae;Han, Gyu-Heung;Woo, In-Shik
    • Plant Resources
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    • v.5 no.3
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    • pp.176-180
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    • 2002
  • This study was conducted to develop the clonal propagation technique through in vitro culture using by leaf sheath explants of Phalaenopsis grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when explants of leaf sheath were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 mVL coconut water, 1 mg/L NAA, 1 mg/L 2ip and 2.5 g/L gelrite. The PLB formation rate of VW medium was highest followed by modified Hyponex medium, and lowest in MS medium. Plantlets induced from PLBs transferred to plastic pots including spagnum moss were well developed.

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Light-susceptibility of Camptothecin Production from in Vitro Cultures of Camptotheca acuminata Decne

  • Park, Young-Goo;Park, Mee-Hee;Yang, Jae-Kyung;Chung, Young-Gwan;Park, Myung-Suk
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.1
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    • pp.32-36
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    • 2003
  • Production of camptothecin ((PT) from callus cultures of Camptotheca acuminata Decne was affected by light and culture conditions. Among the culture media tested, modified B5 medium containing 3% (w/v) sucrose, 2 mg/L B,4-D, 2 times of MS medium vitamins, 500 mg/L casein hydrolysate, 250 mg/L myo-inositol, 0.05% (w/v) activated charcoal, and 0.15% (w/v) gelite was used for callus induction . The highest cell growth and CPT production were obtained in dark and green light condition, respectively. Photoperiod has no effect on cell growth and CPT production. Both cell growth and CPT production were also influenced by combination ratio of red and blue light .Cell growth and CPT production were the highest in the ratio of red and blue light,90:10.