• Korean Journal of Medicinal Crop Science
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• v.27 no.6
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• pp.397-403
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• 2019

Background: Oplopanax elatus is widely distributed at high altitudes (about 1,100 m) in China, Russia and Korea. It is hard to propagate, breed, and difficult to grow. Hence, it has been designated as a rare and endangered medicinal plant. A study was conducted to establish a system for large scale seedling production of Oplopanax elatus in vitro and to find the ideal environment for its seedling growth. Methods and Results: In this study, the explants produced under in vitro conditions during our previous study were grouped into three categories (under 10 mm, 10 mm - 30 mm and above 30 mm) based on plant height and were transferred to the growth-chamber and greenhouse for two weeks in each setting for acclimatization. The plantlet category of above 30 mm showed good performance, and was further evaluated under three acclimatization methods as follows: three different growth media (commercial soil, commercial soil + perlite, commercial soil + sand), four shading levels (0%, 50%, 70%, 90%) and four altitude levels (157 m, 218 m, 601 m, 870 m) in Gangwon province of South Korea. As results, O. elatus seedlings showed better growth characteristics at 870 m of altitude, 70% shading level and in the commercial soil compared to other treatments. Conclusions: The regenerated seedlings of Oplopanax elatus obtained through plant tissue culture would be advantageous for use in large scale seedling production systems paired with a good acclimation method. For obtaining optimal results, it is recommended that seedling be acclimatized in a high altitude environment.

• The Journal of Dong Guk Oriental Medicine
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• v.9
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• pp.127-138
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• 2000

The methods of checking the exuberance of yang (潛陽法) are a kind of medical treatments which cure various symtoms caused by hyperactivity of liver-yang, at the same time used for treating apoplexy. Thus, I investigated the application of checking the exuberance of yang to treat stroke. The conclusions are as follows. We can find application of checking the exuberance of yang to apoplexy on Synopsis of the Gold Chamber. After Ye Gui who persisted the theory of endogenous wind, many physicians applied the method of checking the exuberance of yang and this method has usually applied with nourishing yin. The methods of checking the exuberance of yang are concretely applied as follows. 1) a portion of apoplexy involving the channels and collaterals, which is caused by deficiency of yin and wind-fire pathogen. 2) excessive-syndrome of coma accompanied by heat syndrome. 3) epilepsy caused old apoplexy. The majority of shells for example abalone shell, hawksbill shell, oyster shell, tortoise-shell etc. are the most frequent use of herbal drug which check the exuberance of yang. The precriptions of checking the exuberance of yang are Ho-jam-hwan(虎潛丸), Ko-bon-dan(固本丹), Cheon-ma-gu-deung-eum(天麻鉤藤飮), Chin-gan-sik-pung-tang(鎭肝熄風湯) etc.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.135-135
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• 2015

Tubes are of extreme importance in industries as for fluid channels or wave guides. Furthermore, some weapon systems such as cannons use the tubes as gun barrels. To increase the service life of such tubes, a protective coating must be applied to the tubes' inner surface. However, the coating methods applicable to the inner surface of the tubes are very limited due to the geometrical restriction. A small-diameter cylindrical magnetron sputtering gun can be used to deposit coating layers on the inner surface of the large-bore tubes. However, for small-bore tubes with the inner diameter of one inch (~25 mm), the magnetron sputtering method can hardly be accommodated due to the space limitation for permanent magnet assembly. In this study, a new approach to coat the inner surface of small-bore tubes with the inside diameter of one inch was developed. Instead of using permanent magnets for magnetron operation, an external electro-magnet assembly was adopted around the tube to confine the plasma and to sustain the discharge. The electro-magnet was operated in pulse mode to provide the strong axial magnetic field for the magnetron operation, which was synchronized with the negative high-voltage pulse applied to the water-cooled coaxial sputtering target installed inside the tube. By moving the electro-magnet assembly along the tube's axial direction, the inner surface of the tube could be uniformly coated. The inner-surface coating system in this study used the tube itself as the vacuum chamber. The SS-304 tube's inner diameter was 22 mm and the length was ~1 m. A water-cooled Cu tube (sputtering target) of the outer diameter of 12 mm was installed inside of the SS tube (substrate) at the axial position. The 50 mm-long electro-magnet assembly was fed by a current pulse of 250 A at the frequency and pulse width of 100 Hz and 100 usec, respectively. The calculated axial magnetic field strength at the center was ~0.6 Tesla. The central Cu tube was synchronously driven by a HiPIMS power supply at the same frequency of 100 Hz as the electro-magnet and the applied pulse voltage was -1200 V with a pulse width of 500 usec. At 150 mTorr of Ar pressure, the Cu deposition rate of ~10 nm/min could be obtained. In this talk, a new method to sputter coat the inner surface of small-bore tubes would be presented and discussed, which might have broad industrial and military application areas.

• Korean Journal of Optics and Photonics
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• v.8 no.4
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• pp.283-290
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• 1997

Scattering measurement on high reflection dielectric multilayer mirrors deposited on quartz substrate in a vacuum chamber were performed using a total integrated scattering method. Scattering of (Ta$_2$$O_5$/SiO$_2$) multilayer mirrors deposited at 250-30$0^{\circ}C$ was 0.048-0.050% and did not change with an annealing at 30$0^{\circ}C$ for 4 hours. On the other hand, scattering of (TiO$_2$/SiO$_2$) multilayer mirror at 25$0^{\circ}C$ was 0.029% and it showed the heavy tensile stress after an annealing. The rms roughness of (Ta$_2$$O_5$/SiO$_2$) multilayer mirror was almost the same as that of (TiO$_2$/SiO$_2$)multilayer mirror. The column size of Ta$_2$$O_5$ film was smaller than that of TiO$_2$film and the packing density of (Ta$_2$$O_5$/SiO$_2$) multilayer mirrors was higher than that of (TiO$_2$/SiO$_2$) multilayer mirror. It seems that the higher packing density and smaller column size of Ta$_2$$O_5$ films lead to more scattering.

• Journal of Advanced Marine Engineering and Technology
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• v.8 no.1
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• pp.17-36
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• 1984

Since 1973, the competition on the development of fuel saving type internal combustion engines has become severe by the two times oil shock, and new type engines are reported every several months. Whenever these new type engines are developed, new designs are required and they will be offered in the market after performing the endurance test for a long time. But the engine market is faced with a heavy burden of finance, as the developing of a new engine requires tremendous expenses. For this reason, the computer simulation method has been lately developed to cope with it. The computer simulation method can be available to perform the reasonable research works by the theoretical analysis before carrying out practical experiments. With these processes, the developing expenses are cut down and the period of development is curtailed. The object of this study is the development of simulation computer program for the small naturally aspirated four-stroke diesel engine which is intended to product by the original design of our country. The process of simulation is firstly investigated for the ideal engine cycle, and secondly for the real engine cycle. In the ideal engine cycle, each step of the cycle is simulated by the energy balance according to the first law of thermodynamics, and then the engine performance is calculated. In the real cycle imulation program, the injection rate, the preparation rate and the combustion rate of fuel and the heat transfer through the wall of combustion chamber are considered. In this case, the injection rate is supposed as constant through the crank angle interval of injection and the combustion rate is calculated by the Whitehouse-Way equation and the heat transfer is calculated by the Annand's equation. The simulated values are compared with measured values of the YANMAR NS90(C) engine and Mitsubishi 4D30 engine, and the following conclusions are drawn. 1. The heat loss by the exhaust gas is well agree with each other in the lower load, but the measured value is greater than the calculated value in the higher load. The maximum error rate is about 15% in the full load. 2. The calculated quantity of heat transfer to the cooling water is greater than the measured value. The maximum error rate is about 11.8%. 3. The mean effective pressure, the fuel consumption, the power and the torque are well agree with each other. The maximum error is occurred in the fuel consumption, and its error rate is about 7%. From the above remarks, it may be concluded that the prediction of the engine performance is possibly by using the developed program, although the program needs to reform by adding the simulation of intake and exhaust process and assumping more reliable mechanical efficiency, volumetric efficiency, preparation rate and combustion rate.

• Korean Journal of Materials Research
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• v.17 no.1
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• pp.31-36
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• 2007

Nanoimprint lithography (NIL) is a new lithographic method that offers a sub-10nm feature size, high throughput, and low cost. One of the most serious problems of NIL is the stiction between mold and resist. The antistiction layer coating is very effective to prevent this stiction and ensure the successful NIL results. In this paper, an antistiction layer was deposited by VSAM (vapor self assembly monolayer) method on silicon samples with FOTS (perfluoroctyltrichlorosilane) as a precursor for making an antistiction layer. A specially designed LPCVD (low pressure chemical vapor deposition) was used for this experiment. All experiments were achieved after removing the humidity. First, the evaporation test of FOTS was performed for checking the evaporation temperature at low pressure. FOTS was evaporated at 5 Tow and $110^{\circ}C$. In order to evaluate the temperature effect on antistiction layer, chamber temperature was changed from 50 to $170^{\circ}C$ with 0.1ml of FOTS for 1 minute. Good hydrophobicity of all samples was shown at about $110^{\circ}$ of contact angle and under $20^{\circ}$ of hysteresis. The surface energies of all samples calculated by Lewis acid/base theory was shown to be about 15mN/m. The deposited thicknesses of all samples measured by ellipsometry were almost 1nm that was similar value of the calculated molecular length. The surface roughness of all samples was not changed after deposition but the friction force showed relatively high values and deviations deposited at under $110^{\circ}$. Also the white circles were founded in LFM images under $110^{\circ}$. High friction forces were guessed based on this irregular deposition. The optimized VSAM process for FOTS was achieved at $170^{\circ}C$, 5 Torr for 1 hour. The hot embossing process with 4 inch Si mold was successfully achieved after VSAM deposition.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.2
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• pp.173-181
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• 2015

Fluxes of carbon dioxide ($CO_2$) were measured above crop canopy using the Eddy Covariance (EC) method, and emission rate of methane ($CH_4$) was measured using Automatic Open/Close Chamber (AOCC) method during the 2012-2013 barley and rice growing season in a barley-rice double cropping field of Gimje, Korea. The net ecosystem exchange (NEE) of $CO_2$ in the paddy field was analyzed to be affected by crop growth (biomass, LAI, etc.) and environment (air temperature, solar radiation, etc.) factors. On the other hand, the emission rate of $CH_4$ was estimated to be affected by water management (soil condition). NEE of $CO_2$ in barley, rice and fallow period was -100.2, -374.1 and $+41.2g\;C\;m^{-2}$, respectively, and $CH_4$ emission in barley and rice period was 0.2 and $17.3g\;C\;m^{-2}$, respectively. When considering only $CO_2$, the barley-rice double cropping ecosystem was estimated as a carbon sink ($-433.0g\;C\;m^{-2}$). However, after considering the harvested crop biomass ($+600.3g\;C\;m^{-2}$) and $CH_4$ emission ($+17.5g\;C\;m^{-2}$), it turned into a carbon source ($+184.7g\;C\;m^{-2}$).

• Analytical Science and Technology
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• v.14 no.5
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• pp.451-457
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• 2001

Because liquids with high molecular weight such as mineral oil have low vapor pressure at room temperature, it is generally thought to be difficult to lose them to evaporation. However, when they are dispersed into air in small droplets during application in machining processes, their surface area becomes considerably higher. To determine the potential for metalworking fluids (MWF) filter losses, MWF mist was generated and collected on polyvinyl chloride (PVC) filters in test chamber. After collected MWF was exposed to clean air during designated period (range 10~240 minutes) and the filters were desiccated, losses were evaluated. As duration of clean air passing through PVC filter increased, loss of MWF gradually increased. MWF lost after 10 minutes ranged form 12.4 % to 21.8 % of the original loading mass, on average 53.3 % of the total loss. These results indicate that significant mass of MWF collected on PVC filters can be lost at the beginning of air sampling. Loss of MWF collected on PVC filter also occurred during desiccation without active airflow. In multiple regression to identify which factors influence the loss of MWF collected on PVC filter, both duration of air passing through PVC filter and MWF age (fresh vs. used) were significant predictor (p=0.0001). Therefore, workers' exposure to MWF measured method 0500, may underestimate true concentration. Further study is needed to develop a new method to quantify the workers' exposure to airborne MWF mist accurately.

• Korean journal of applied entomology
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• v.58 no.4
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• pp.341-345
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• 2019

Ethyl formate (EF) is a rapid kill, environmentally safe, and low mammalian toxicity fumigant, registered to disinfest quarantine insect pests from imported agricultural products. A new concept for controlling insect pests of agricultural crops was tested in a fumigation chamber with EF. Control efficacy of and phyto-toxicity due to EF fumigation were evaluated against four pests (Thrips palmi, Bemisia tabaci, Myzus persicae, and Tetranychus urticae) and on seedlings of four fruit vegetables (FVs; yellow melon, cucumber, tomato, and pepper). Ethyl formate fumigation at a dose of 1.5 g m^-3 for 12 h produced >93.3% mortality in T. palmi, B. tabaci, and M. persicae. However, T. urticae was tolerant to fumigation, showing only 20% mortality at 2.0 g m^-3. In terms of concentration × time (CT) products, at least 8.9 g·h m^-3 CT at 20 ± 1.5℃ was needed to achieve > 90% mortality against the three susceptible insect pests. Fumigation at 1.5 g m^-3 for 12 h caused no phyto-toxicity to any of the four FV seedlings. Ethyl formate application, as a new disinfestation method in greenhouses, could be an alternative to reduce the use of conventional insecticides. However, further studies are needed to determine the efficacy of this method at different pest developmental stages and in different greenhouse environments. Additionally, research is needed to elucidate the phyto-toxicity of EF application at different growing stages of a wide variety of crops.

• The Journal of Internal Korean Medicine
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• v.25 no.3
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• pp.461-472
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• 2004

Objectives : For the screening of neuroprotective effects of medicinal herbs, the complex system of animal models suffer some disadvantages in controlling critical parameters such as blood pressure and body temperature. Additionally, application of drugs to the appropriate brain area sometimes is difficult, due to poor permeability though the blood brain barrier, and so potential protective effects might be masked. Methods : Organotypic hippocampal slice culture (OHSC) method has the advantages of being relatively easy to prepare and of maintaining the general structure, including tissue integrity and the connections between cells. Drugs can easily be applied and neuronal damage can easily be quantified by using tissues and culture media. This study demonstrates neuroprotective effects of Puerariae radix (葛根, PR), Salviae miltiorrhizae radix (丹蔘, SR), Rhei rhizoma (大黃, RR), and Bupleuri radix (柴胡, BR). These were screenedand compared to MK-801, antagonist of NMDA receptors, by using OHSC of 1 week-old Sprague-Dawley rats. Oxygen/glucose deprivation (OGD) were conducted in an anaerobic chamber $(85%\;N_2,\;10%\;CO_2\;and\;5%\;H_2)$ in a deoxygenated glucose-free medium for 60 minutes. Water extracts of each herbs were treated to culture media with $5\;{\mu}g/ml$ for 48 hours. Results : Neuronal cell death in the cultures was monitored by densitometric measurements of the cellular uptake of propidium iodide (PI). PI fluorescence images were obtained at 48 hours after the OGD and medicinal herb treatment. Also TUNEL-positive cells in the CAI and DG regions and LDH concentrations in culture media were measured at 48 hours after the OGD. According to measured data, MK-801, PR, SR and BR demonstrated significant neuroprotective effect against excessive neuronal cell death and apoptosis induced by the OGD insult. Especially, PR revealed similar neuroprotective effect to MK-801 and RR demonstrated weak neuroprotective effect. Conclusions : These results suggest that OHSC can be a suitable method for screening of neuroprotective effects of medicinal herbs. (This work was supported by the research program of Dongguk University and Grant 01-PJ9-PG1-01CO03-0003 from Ministry of Health & Welfare.)