• Journal of Life Science
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• v.25 no.2
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• pp.136-150
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• 2015

Pseudomonas syringae pathovar tabaci is a plant pathogenic bacterium that causes wildfire disease in tobacco plants. In P. syringae pv. tabaci, PsyI, a LuxI-type protein, acts as an AHL synthase, while primary and secondary sequence analysis of PsyR has revealed that it is a homolog of the LuxR-type transcriptional regulator that responds to AHL molecules. In this study, using phenotypic and genetic analyses in P. syringae pv. tabaci, we show the effect of PsyR protein as a quorum-sensing (QS) transcriptional regulator. Regulatory effects of PsyR on swarming motility and production of siderophores, tabtoxin, and N-acyl homoserine lactones were examined via phenotypic assays, and confirmed by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Further qRT-PCR showed that PsyR regulates expression of these virulence genes in response to environmental signals. However, an upstream region of the gene was not bound with purified MBP-PsyR protein; rather, PsyR was only able to shift the upstream region of psyI. These results suggested that PsyR may be indirectly controlled via intermediate-regulatory systems and that auto-regulation by PsyR does not occur.

• Journal of Life Science
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• v.31 no.12
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• pp.1100-1109
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• 2021

The halophyte Artemisia scoparia is an edible medicinal plant, with insecticidal, anti-inflammatory, anticholesterol, antipyretic, and antibacterial effects. The aim of this study was to assess the inhibitory effect of crude extract and solvent-partitioned fractions obtained from A. scoparia on MMP-2 and MMP-9 activity in phorbol-12-myristate-13-acetate (PMA)-stimulated human fibrosarcoma HT-1080 cells using four different activity tests: gelatin zymography, MMP enzyme-linked immunosorbent assay (ELISA), wound healing assay, reverse transcription-polymerase chain reaction (RT-PCR), and Western blot assay. A. scoparia samples were extracted twice with methylene chloride (MC) and twice with methanol (MeOH). After the MC and MeOH crude extracts were combined, the combined crude extracts showed a significant inhibitory effect against MMP-2 and MMP-9 enzymes. They were then fractionated into n-hexane, 85% (v/v) aqueous methanol (85% (v/v) aq.MeOH), n-butanol, and water according to solvent polarity. Among the four solvent-partitioned fractions, n-hexane and 85% (v/v) aq. MeOH fractions significantly inhibited MMP-2 and MMP-9 activity and cell mobility. In addition, the n-hexane and 85% (v/v) aq.MeOH fractions effectively inhibited MMP-2 and -9 activity in the gelatin zymography and MMP ELISA assay. In the wound healing assay, RT-PCR, and Western blot assay, all solvent-partitioned fractions, except the H₂O fraction, significantly suppressed cell migration, as well as the expression levels of MMP-2 and -9 mRNA and proteins.

• Safety and Health at Work
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• v.7 no.4
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• pp.381-388
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• 2016

Background: Relationships among portable scanning mobility particle sizer (P-SMPS), condensation particle counter (CPC), and surface area monitor (SAM), which are different metric measurement devices, were investigated, and two widely used research grade (RG)-SMPSs were compared to harmonize the measurement protocols. Methods: Pearson correlation analysis was performed to compare the relation between P-SMPS, CPC, and SAM and two common RG-SMPS. Results: For laboratory and engineered nanoparticle (ENP) workplaces, correlation among devices showed good relationships. Correlation among devices was fair in unintended nanoparticle (UNP)-emitting workplaces. This is partly explained by the fact that shape of particles was not spherical, although calibration of sampling instruments was performed using spherical particles and the concentration was very high at the UNP workplaces to allow them to aggregate more easily. Chain-like particles were found by scanning electron microscope in UNP workplaces. The CPC or SAM could be used as an alternative instrument instead of SMPS at the ENP-handling workplaces. At the UNP workplaces, where concentration is high, real-time instruments should be used with caution. There are significant differences between the two SMPSs tested. TSI SMPS showed about 20% higher concentration than the Grimm SMPS in all workplaces. Conclusions: For nanoparticle measurement, CPC and SAM might be useful to find source of emission at laboratory and ENP workplaces instead of P-SMPS in the first stage. An SMPS is required to measure with high accuracy. Caution is necessary when comparing data from different nanoparticle measurement devices and RG-SMPSs.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.1
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• pp.190-197
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• 2010

The ACR (Access Control Router) of WiBro processes location registration of the Correspondent Node and Home Agent as the Correspondent Node moves between ACRs. Therefore, the location update cost is low compared with MIPv6. However, all packets which are sent and received are sent through the ACR, so as the number of mobile nodes that are managed by the ACR increases, the cost of packet delivery also increases. Therefore, the communication state of the ACR domain remains smooth when the ACR which manages the mobile node in the ACR domain has good performance. However, network delays occur unless the ACR performs well, so the role of the ACR is important. In this paper, we analysis performance of the ACR for efficient realization of the WiBro standard. By using the Deny Probability and the Total Profit of ACR performance and apply it to the Random Walk Mobility model as the mobility model.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.44 no.1
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• pp.113-122
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• 2007

In this paper, we propose a multi-state based hybrid location update scheme, which integrates the time-based and the movement-based methods. In the proposed scheme, a mobile terminal updates its location after n cell boundary crossing and a time interval of T[sec]. We derive an analytical solution for the performance of the hybrid scheme with exponential cell resident time and evaluate it numerically with time-varying random walk mobility model, which we model as multi-states Markov chain. Furthermore, we also evaluate the scheme for arbitrary cell resident times by simulation. From the numerical analysis and the simulation results, we prove that the proposed scheme significantly outperforms the time-based and the movement-based methods, when implemented alone, more accurately adapting to the time-varying user mobility.

• Proceedings of the Membrane Society of Korea Conference
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• 2004.05a
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• pp.82-85
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• 2004

Microinterface of W/Omicroemulsion prepared by phosphatidylcholine was used as reaction media of hydrolysis of phosphatidylcholine by phospholipaseA$_2$. Phosphatidylcholine was used as an amphiphile and was acted as a substrate. Organic phase of W/Omicroemulsion in this study was prepared by mixed organic solvents i.e. 2,2,4-trimethylpentane (isooctane) as a main solvent and 1-butanol as a co-solvent. The effect of added 1-butanol was remarkable not only on reaction beginning but also on high reaction rate. The hydrolysis reaction was dramatically initiated when 1-butanol was injected into the running isooctane/PC system. The enhancement by 1-butanol addition into single organic solvent was our original finding compare with previous conventional organic solvent. The reaction rate was elevated by the added amount of 1-butanol. The enhanced reaction rate was about 150-folds. This enhancement was speculated as 1-butanol adsorption on the microinterface. The adsorbed 1-butanol improved the properties of microinterface, especially its mobility was increased by difference of the chain length between phosphatidylcholine and 1-butanol. PhospholipaseA$_2$ molecules were located on the microinterface due to modified mobility of microinterface. Located phospholipaseA$_2$ on the microinterface reacted easily with phosphatidylcholine molecule. As a result high reaction rate was obtained. Microinterfacial properties were successfully improved by adsorbed 1-butanol molecule, and were favorable to appear higher reactivity of phospholipaseA$_2$.

• Bulletin of the Korean Chemical Society
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• v.29 no.6
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• pp.1167-1172
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• 2008

We investigated the glass transition temperatures ($T_g$) of dendrons consisting of conformationally mobile aliphatic polyether dendritic cores plus glassy peripheral polystyrenes (PSs), and linear PSs in the molecular weight range of 1000-8500 g/mol. We compared their $T_g$ behavior depending on their polymeric architecture. The linear PSs show a typical growth of $T_g$ up to 92.5 ${^{\circ}C}$ as the molecular weight increases to 8300 g/mol, while the dendrons display nearly constant $T_g$ values of 58-61 ${^{\circ}C}$, despite the increase of molecular weight with each generation. The striking contrast of Tg behavior would be mainly attributed to the fact that the dendrons keep the ratio of $N_e$/M ($N_e$: number of peripheral chain ends, M: molecular weight) over all the generations. Additionally, for the influence of dendritic spacers on glass transition temperature we prepared dimeric PSs with different linkage groups such as aliphatic ether, ester and amide bonds. We found that the dimer with the ether spacer exhibited the lowest glass transition at 55.4 ${^{\circ}C}$, while the amide linked dimer showed the highest glass transition temperature at 74.2 ${^{\circ}C}$. This indicates that the peripheral PS chains are effectively decoupled by the conformationally flexible ether spacer. The results from this study demonstrated that polymeric architecture and dendritic core structures play a crucial role in the determination of glass transition behavior, providing a strategy for the systematic engineering of polymer chain mobility.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.5
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• pp.1146-1165
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• 2013

Collaborative awareness between persons with various smart multimedia devices is a new trend in the Internet of Things (IoT). Because of the mobility, randomness, and complexity of persons, it is difficult to achieve complete data awareness and data transmission in IoT. Therefore, research must be conducted on mobile-aware service models. In this work, we first discuss and quantify the social relationships of mobile nodes from multiple perspectives based on a summary of social characteristics. We then define various decision factors (DFs). Next, we construct a directed and weighted community by analyzing the activity patterns of mobile nodes. Finally, a mobile-aware service routing algorithm (MSRA) is proposed to determine appropriate service nodes through a trusted chain and optimal path tree. The simulation results indicate that the model has superior dynamic adaptability and service discovery efficiency compared to the existing models. The mobile-aware service model could be used to improve date acquisition techniques and the quality of mobile-aware service in the IoT.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.1B
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• pp.11-16
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• 2007

This paper describes the design and performance of a secure routing protocol with time-space cryptography for mobile ad-hoc networks. The proposed time-space scheme works in the time domain for key distribution between source and destination as well as in the space domain for intrusion detection along the route between them. For data authentication, it relies on the symmetric key cryptography due to high efficiency and a secret key is distributed using a time difference from the source to the destination. Also, a one-way hash chain is formed on a hop-by-hop basis to prevent a compromised node or an intruder from manipulating the routing information. In order to evaluate the performance of our routing protocol, we compare it with the existing AODV protocol by simulation under the same conditions. The proposed protocol has been validated using the ns-2 network simulator with wireless and mobility extensions.

• Journal of Communications and Networks
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• v.10 no.3
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• pp.287-296
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• 2008

The IEEE 802.11 wireless standard uses the carrier sense multiple access with collision avoidance (CSMA/CA) as its MAC protocol (during the distributed coordination function period). This protocol is an adaptation of the CSMA/CD of the wired networks. CSMA/CA mechanism cannot guarantee quality of service (QoS) required by the application because orits random access method. In this study, we propose a new MAC protocol that considers different types of traffic (e.g., voice and data) and for each traffic type different priority levels are assigned. To improve the QoS of IEEE 802.11 MAC protocols over a multi-channel CSMA/CA, we have developed a new admission policy for both voice and data traffics. This protocol can be performed in direct sequence spread spectrum (DSSS) or frequency hopping spread spectrum (FHSS). For voice traffic we reserve a channel, while for data traffic the access is random using a CSMA/CA mechanism, and in this case a selective reject and push-out mechanism is added to meet the quality of service required by data traffic. To study the performance of the proposed protocol and to show the benefits of our design, a mathematical model is built based on Markov chains. The system could be represented by a Markov chain which is difficult to solve as the state-space is too large. This is due to the resource management and user mobility. Thus, we propose to build an aggregated Markov chain with a smaller state-space that allows performance measures to be computed easily. We have used stochastic comparisons of Markov chains to prove that the proposed access protocol (with selective reject and push-out mechanisms) gives less loss rates of high priority connections (data and voices) than the traditional one (without admission policy and selective reject and push-out mechanisms). We give numerical results to confirm mathematical proofs.