• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.331-337
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• 2019

Digitaria exilis L. is an under-utilized crop with high nutritional and medicinal values. It thrives in and is well-adapted to arid areas with low soil nutrients. Using biochemical markers, this study investigates the mechanisms by which D. exilis responds to osmotic stress. Three accessions Dinat Iburua (DIN), Jakah Iburua (JAK) and Jiw Iburua (JIW) were collected from National Cereal Research Institute, Niger State. Two accessions, NG/11/JD/061 and NG/11/JD/062 were also collected from National Centre for Genetic Resources and Biotechnology, Ibadan. Murashige and Skoog medium of approximately 1.2 L was supplemented with polyethylene glycol 6000 to create osmotic pressures of -9.29, -13.93, -20.13, -26.32, -32.51, and 0 MPa (control). Sterilized seeds were inoculated in the medium and placed in the growth room for 4 weeks. Proline accumulation was significantly high in all JAK plants under osmotic stress. Proline and ascorbate peroxidase (p<0.05) activities were directly correlated, thus reinforcing the survivability of JAK during stress. Catalase (CAT) activity was also significantly induced in JAK under osmotic stress, which synergistically improved its tolerability. As a result, >50% of OH-, H₂O₂, and NO radicals were scavenged. However, other accessions including DIN, NG061, NG062, and JIW showed variations in their responses to different levels of osmotic stress, although not significant. Therefore, JAK possesses a well-equipped free radical quenching system that is protected by the accumulation of the osmolyte proline; therefore, accession JAK is considered osmotolerant. CAT and superoxide dismutase activities were osmostabilized against oxidative stress by proline.

• The Korean Journal of Food And Nutrition
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• v.19 no.2
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• pp.176-182
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• 2006

Sorghum bicolor L. Moench(Sorghum, Su-Su) is a major cereal food crop used in many parts of the world. It is used as a human food resource and folk medicines in Asia and Africa. The stem of sorghum has been used as a digestive aid and an anti-diarrheal agent. Sorghum hybrids contain high levels of diverse phenolic compounds that may provide health benefits. High levels of polyflavanols, anthocyanins, phenolic acids, and other antioxidant compounds have been reported in sorghums, which have also been shown to possess various biological activities such as anti-mutagenic, anti-carcinogenic, and HMG-CoA reductase inhibitory activities. In an in vitro experiment, we examined mice splenocyte proliferation and production of three types of cytokine($IL-1{\beta},\;IL-6,\;TNF-{\alpha}$) by peritoneal macrophages cultured with ethanol and water extracts of Sorghum bicolor L. Moench. A single cell suspension of splenocytes was prepared and the cell proliferation of the splenocytes was examined by MTT assay. The splenocyte proliferation was increased when water extracts of Sorghum bicolor L. Moench were used as supplements in all concentrations investigated. The production of cytokine($IL-1{\beta},\;IL-6,\;TNF-{\alpha}$) by activated peritoneal macrophage was detected by ELISA using the cytokine kit. $IL-1{\beta},\;IL-6,\;and\;TNF-{\alpha}$ production by activated macrophages were increased by supplementation with Sorghum bicolor L. Moench water extracts. This study suggests that supplementation of with Sorghum bicolor L. Moench water extracts may enhance immune function by regulating the splenocyte proliferation and enhancing the cytokine production by activated macrophages in vitro.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.5
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• pp.643-648
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• 2005

Near infrared reflectance spectroscopy (NIRS) has become increasingly used as a rapid, accurate method of evaluating some chemical constituents in cereal grains and forages. If samples could be analyzed without drying and grinding, then sample preparation time and costs may be reduced. This study was conducted to develop robust NIRS equations to predict fermentation quality of corn (Zea mays) silage and to select acceptable sample preparation methods for prediction of fermentation products in corn silage by NIRS. Prior to analysis, samples (n = 112) were either oven-dried and ground (OD), frozen in liquid nitrogen and ground (LN) and intact fresh (IF). Samples were scanned from 400 to 2,500 nm with an NIRS 6,500 monochromator. The samples were divided into calibration and validation sets. The spectral data were regressed on a range of dry matter (DM), pH and short chain organic acids using modified multivariate partial least squares (MPLS) analysis that used first and second order derivatives. All chemical analyses were conducted with fresh samples. From these treatments, calibration equations were developed successfully for concentrations of all constituents except butyric acid. Prediction accuracy, represented by standard error of prediction (SEP) and $R^2_{v}$ (variance accounted for in validation set), was slightly better with the LN treatment ($R^2$ 0.75-0.90) than for OD ($R^2$ 0.43-0.81) or IF ($R^2$ 0.62-0.79) treatments. Fermentation characteristics could be successfully predicted by NIRS analysis either with dry or fresh silage. Although statistical results for the OD and IF treatments were the lower than those of LN treatment, intact fresh (IF) treatment may be acceptable when processing is costly or when possible component alterations are expected.

• Journal of Food Hygiene and Safety
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• v.32 no.4
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• pp.275-283
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• 2017

We inspected contaminations of pesticides in the 3,313 crop samples grown under the standard of Korean Good Agricultural Practices (GAP) between 2014 to 2016 May. According to our inspection, violations by unregistered pesticide contaminations far outweighed those by exceeding maximum residue limits. Most of the crops contaminated with unregistered pesticides were minor vegetable crops, for which far less pesticide products are registered compared to cereal grains and fruits. And in our second study, the pesticide in the sprayer was removed 90% or more, but not totally by serial rinses. From this result, it is assumed that pesticide remained in the rinsed sprayer can be carried over to crops unintentionally. Our study shows that supplying pesticide products evenly to all kinds of crops and allowing unintentional carry-over of unregistered pesticides to a certain extent are solutions to revitalize depressed Korean Good Agricultural Practices.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.4
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• pp.316-323
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• 1994

In order to reduce the problems from the presence of rye crop residues in maize establishment and yield, and also to improve the growth, productivity and nutritive value of rye(Seca1e cereal L.). an experiment was canied out to determine the variety effect of rye on the forage production system and the eft'ect of seeding and harvesting dates on the production and quality of rye. 'This experiment was conducted at the forage testing field of S.N.U., Suweon, from September 1986 to May 1989. Heading date of an early maturing rye variety, Wintermore. was earlier 10 days than that of a late maturing rye variety, Kodiak. A 15-days delay in the seeding dates of early and late maturing varieties of rye tended to delay the heading dates of the rye varieties for 3 and 4 days, respectively. Dry matter and in vitro digestible dry matter yields were markedly increased with earlier seeding date. Before 20 April, the DM and IVDDM yeilds of an early maturing rye variety, Winterrnore. were higher than those of a late maturing rye variety, Kodiak. However, no such a trend was found between the early and late maturing varieties of rye after 27 April. Less than 35% of ADF was recorded until the harvesting dates of 13 and 27 p r i l for early and late maturing rye varieties, respectively, but less than 46% of NDF was maintained until the harvesting drtte of 13 April for botg varieties. Based on the results obtained from this experiment. it may be concluded that the most desirable forage production from corn-rye double cropping system is to advance the seeding time of rye toward the first 10 days of September as well as harvesting time toward the first heading stage with an early maturing rye variety.

• 한국균학회소식:학술대회논문집
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• 2016.05a
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• pp.27-27
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• 2016

The ascomycete fungus Fusarium graminearum is the most common pathogen of Fusarium head blight (FHB), a devastating disease for major cereal crops worldwide. FHB causes significant crop losses by reducing grain yield and quality as well as contaminating cereals with trichothecenes and zearalenone (ZEA) that pose a serious threat to animal health and food safety. ZEA is a causative agent of hyperestrogenic syndrome in mammals and can result in reproductive disorders in farm animals. In F. graminearum, the ZEA biosynthetic cluster is composed of four genes, PKS4, PKS13, ZEB1, and ZEB2, which encode a reducing polyketide synthase, a nonreducing polyketide synthase, an isoamyl alcohol oxidase, and a transcription factor, respectively. Although it is known that ZEB2 primarily acts as a regulator of ZEA biosynthetic cluster genes, the mechanism underlying this regulation remains undetermined. In this study, two isoforms (ZEB2L and ZEB2S) from the ZEB2 gene in F. graminearum were characterized. It was revealed that ZEB2L contains a basic leucine zipper (bZIP) DNA-binding domain at the N-terminus, whereas ZEB2S is an N-terminally truncated form of ZEB2L that lacks the bZIP domain. Interestingly, ZEA triggered the induction of both ZEB2L and ZEB2S transcription. In ZEA producing condition, the expression of ZEB2S transcripts via alternative promoter usage was directly or indirectly initiated by ZEA. Physical interaction between ZEB2L and ZEB2L as well as between ZEB2L and ZEB2S was observed in the nucleus. The ZEB2S-ZEB2S interaction was detected in both the cytosol and the nucleus. ZEB2L-ZEB2L oligomers activated ZEA biosynthetic cluster genes, including ZEB2L. ZEB2S inhibited ZEB2L transcription by forming ZEB2L-ZEB2S heterodimers, which reduced the DNA-binding activity of ZEB2L. This study provides insight into the autoregulation of ZEB2 expression by alternative promoter usage and a feedback loop during ZEA production.

• Food Science and Preservation
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• v.17 no.5
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• pp.667-673
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• 2010

Effects of substituting wheat flour with rice flour on bread-making properties were investigated. Rice(Oryza sativa L.) cultivars were 'Goami2(G2)', a functional rice containing higher non-digestible carbohydrates, and 'Chucheongbyeo (CC)', an ordinary Japonica rice. Rice flour was substituted at 10, 20 and 30% in wheat bread formulation, and the composite flour was used for yeast-leavened bread making. Peak and final viscosity of G2 rice flour was significantly lower than that of CC rice flour. Mixograph analysis indicated that replacement of G2 rice flour increased dough water absorption and mixing time compared to wheat flour. But, typical mixograph pattern was not found in the dough from composite flour with CC rice flour. As increasing the amount of rice flour, the G2 rice breads showed a significant decrease in loaf volume, but an increase in bread crumb firmness(g). For the CC rice breads, no significant difference(p<0.05) was observed in loaf volume and crumb firmness, ranging 1012~1050 cc and 433~482g, respectively. The results revealed that Chucheongbyeo is more suitable for bread-making with the composite flour of rice(10~30%) and wheat flour.

• Plant Breeding and Biotechnology
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• v.5 no.3
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• pp.183-191
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• 2017

Finger millet (Eleusine coracana L. Gaertn.) is an important cereal crop in eastern Africa and southern India with excellent grain storage capacity and the unique ability to thrive in extreme environmental conditions. In this study, we analyzed the genetic diversity and population structure of finger millet using 12 developed microsatellites. By sequencing 815 clones from an SSR-enriched genomic DNA library, we obtained 12 polymorphic SSR markers, which also revealed successful amplicons in finger millet accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 76 finger millet accessions in Asia, Africa, and unknown origins. The number of alleles ranged from 2 to 9, with an average of 3.3 alleles. The mean values of observed heterozygosity and expected heterozygosity were 0.27 and 0.35, respectively. The average polymorphism information content was 0.301 in all 76 finger millet accessions. AMOVA analysis showed that the percentage of molecular variance among the populations was 1%, that among individuals was 5%, and that within individuals was 94%. In STRUCTURE analysis, the 76 finger millet accessions were divided into two subpopulations which had an admixture of alleles. There was a correspondence among PCoA, AMOVA, and population structure. This study may form the basis for a finger millet breeding and improvement program.

• Journal of Biosystems Engineering
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• v.43 no.4
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• pp.320-330
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• 2018

Purpose: The aim of this study was to investigate the harvesting performance of a prototype small combine for buckwheat and adlay. Methods: The prototype small combine was designed and constructed. Its ratio of grain loss, ratio of output components in the grain outlet, and field capacity for harvesting buckwheat and adlay were analyzed through field tests. Results: The prototype small combine required a working width of about 0.6 to 0.7 m to harvest buckwheat. The maximum travel speed was about 0.36 m/. The total ratio of grain loss was about 21.6%, which consisted of 8.8% at the header and 12.8% at the dust outlet. The grain and the material other than grain (MOG) ratios at the grain outlet were 94.1% and 5.9% respectively. In the case of adlay harvest, the maximum working width was about 1.2 m, that is, two rows. The range of maximum travel speed was about 0.45 to 0.46 m/s. When adlay was harvested in one row, the total ratio of grain loss ranged from 36.3 to 42.8% according to the cutting height. The cutting height of 30 cm resulted in a higher total ratio of grain loss than 60 cm and 90 cm. When the cutting height was 60 cm, there was no significant change in the total ratio of grain loss according to the number of working rows and the stage of the primary transmission shift. The total ratio of grain loss ranged from 35.2 to 37.7%. The grain and the MOG ratios at the grain outlet ranged from 93.1 to 95.8% and from 4.2 to 6.9%, respectively. No significant difference was observed in relation to cutting height, number of working rows, and the stage of the primary transmission shift. Conclusions: The prototype small combine for harvesting miscellaneous cereal crops showed good potential for the efficient harvesting of buckwheat and adlay. However, to improve the harvesting performance, there seems to be a need to develop new crop varieties suitable for machine-based harvesting and improve the transmissions, reels, separation/cleaning systems.

• International Journal of Computer Science & Network Security
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• v.21 no.3
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• pp.191-197
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• 2021

Ensuring the economic security of agro-industrial complexes of Ukrainian regions has become a top-priority task of state regional policy, as their stable functioning is an essential element of economic security of the whole country. It is overcoming threats to the development of the agro-industrial complex that ensures its further effective functioning and has a significant impact on the economic security of our state. Methods: logical method; methods of system analysis; synthesis; economic and statistical method; method of expert assessment; SWOT analysis; economic and mathematical modelling and planning. Results. Characteristic features of economic security have been given. The essence and significance of the agro-industrial complex in improving the economic security of the state have been determined. It has been noted that in recent years, the agro-industrial complex, which acts as a driver of the domestic economy and has a direct impact on the development of the country, has been growing (in 2019 the cereal and legume harvest exceeded 75 million tons, 20,269 thousand tons of potatoes were dug, more than 15 million tons of sunflower, 9,688 thousand tons of vegetables and 2,119 thousand tons of fruits and berries were harvested, meat and egg production increased by 137.5 thousand tons (or 5.8%) and 545.5 million pieces (or 3.4%), respectively, the number of employed population in agriculture increased by 139.8 thousand people (or 4.9%), the labour productivity in crop production increased by UAH 294.4 thousand (or 44.6%), in livestock production - by UAH 311.3 thousand (or 61.8%)). Based on the system of production and economic indicators, the analysis of the state of the agro-industrial complex has been carried out. Taking into account the results of the obtained data and using SWOT-analysis, the major threats to the development of the agro-industrial complex have been identified. Ways of overcoming threats enhancing the economic security of Ukraine have been proposed.