• Title/Summary/Keyword: centrifuge study

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Protease Inhibitors in Porcine Colostrum: Potency Assessment and Initial Characterization

  • Zhou, Q.;He, R.G.;Li, X.;Liao, S.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.12
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    • pp.1822-1829
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    • 2003
  • Porcine colostrum and milk were separated into the acid-soluble and casein fractions by acidification followed by centrifuge. The acid-soluble fraction of porcine colostrum was further separated by liquid chromatography and anisotropic membrane filtration. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components was determined by incubating bovine trypsin or chymotrypsin in a medium containing their corresponding substrates with or without addition of various amounts of porcine colostrum, porcine milk or their components. The inhibition of insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) degradation in pig small intestinal contents by porcine colostrum was measured by incubating iodinated IGF-I or EGF with the intestinal contents with or without addition of porcine colostrum. Degradation of labeled IGF-I or EGF was determined by monitoring the generation of radioactivity soluble in 30% trichloroacetic acid (TCA). The results showed that porcine colostrum had high levels of trypsin and chymotrypsin inhibitory activity and increased the stability of IGF-I and EGF in pig intestinal contents. The inhibitory activity declined rapidly during lactation. It was also found that trypsin and chymotrypsin inhibitory activity and the inhibition on IGF-I and EGF degradation in the acid-soluble fraction were higher than that in the casein fraction. Heat-resistance study indicated that trypsin inhibitors in porcine colostrum survived heat treatments of $100^{\circ}C$ water bath for up to 10 min, but exposure to boiling water bath for 30 min significantly decreased the inhibitory activity. Compared with the trypsin inhibitors, the chymotrypsin inhibitors were more heatsensitive. Separation of the acid-soluble fraction of porcine colostrum by liquid chromatography and anisotropic membrane filtration revealed that the trypsin and chymotrypsin inhibitory capacity was mainly due to a group of small proteins with molecular weight of 10,000-50,000. In conclusion, the present study confirmed the existence of high levels of protease inhibitors in porcine colostrum, and the inhibition of porcine colostrum on degradation of milk-borne growth factors in the pig small intestinal tract was demonstrated for the first time.

ANALYSIS OF $PGE_2$ CONCENTRATION IN SYNOVIAL FLUID ASPIRATES FROM TMJS OF PATIENTS WITH MANDIBULAR FRACTURE (하악골절 환자에서 악관절강내 활액의 $PGE_2$ 농도 분석)

  • Lee, Gi-Hyug;Kim, Su-Gwan;Kim, Myung-Soo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.27 no.3
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    • pp.204-208
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    • 2001
  • The purpose of this study is to examine the change of enzymeimmuno-assay for $prostaglandinE_2$ in the synovial fluid lavage specimen of patients with mandibular fracture patients without condylar fracture. For this study, fourteen patients (eight males, six females) with mandibular fractures without condylar fracture was investigated to analyse the synovial fluid from upper temporomandibular joint cavities. Synovial fluid was collected from TMJ cavities of mandibular fracture patients before open reduction and after one week of open reduction, and then stored in liquid nitrogen tank after centrifuge. Two synovial fluid lavage samples of TMJs of 2 asymptomatic served as normal controls referred from other data. The concentrations of $PGE_2$ were measured by use of $PGE_2$ EIA System ($Amersham^{(R)}$). The following results were obtained: 1) In nine patients, the concentrations of $PGE_2$ are lower after open reduction than before. 2) In three patients, the concentrations of $PGE_2$ are higher after open reduction than before. 3) There was no statistical significant between the preoperative group and postoperative 7 days (p>0.05), but there was some difference between the two groups. In conclusion, the results suggest that $PGE_2$ probably does not play as important role in the harm of TMJ.

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Evaluation of Giarydia lumblia Detection Method in Stool Specimens Fixed with SAF Solution (SAF 고정변에서 람블편모충(Giardia lamblia)시스트 검출을 위한 검사방법의 평가)

  • Kim, Yeong-Chang;Kim, Jae-Jin;Lee, Geun-Tae
    • Parasites, Hosts and Diseases
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    • v.23 no.2
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    • pp.285-292
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    • 1985
  • The present study has been designed as a basic study on laboratory diagnosis of giardiasis and to demonstrate a more effective method for the detection of Giardia lamblia cyst with the inherent advantages of minimizing both the number of stool examinations required and the interval of stool collections for estimating the real state of prevalence in the shortest time possible. There were 3 subject groups of 75 children each currently residing in an orphanage in Gunsan city, Jeonbuk province from which stool specimens were collected every day, every other day, and every 3 days. The procedure is as follows: 1) resuspend the InKed sample after fixation with SAF solution 2) centrifuge the sediment for 1 min. at 2, 000 rpm after straining through gauze into a tube 3) divide the sediment into 3 parts and use them for direct fecal smear, formalin-ether concentration (MGL) and zinc sulfate ($ZnSO_4$) floatation techniques. The results are summarized as follows: 1. Overall infection rate after 10 trials showed a 60% positive indication. The positive rate among children under 4 years old was significantly higher than the rate in children over 4 years old. No significant difference in rate by sex was observed. 2. The results of eBaminations by direct fecal smear and MGL techniques appeared more accurate than that obtained by $ZnSO_4$ ftoatation method as indicated by a higher positive rate. Of all three methods concerned, combinations of two demonstrated a higher positive rate than that shown by any one alone. 3. In three consecutive examinations under varying conditions such as different days, the cyst detection rate by MGL technique indicated 83%. In 5 examinations under the same varying conditions, the indicated rate was 94%. 4. The interval of stool collection proved to be insignificant for the cyst detection rate. In conclusion, both MGL method and modified fecal direct smear can provide a good cyst detection rate of G. lamblia provided that more than 3 consecutive examinations of stool under varying conditions are carried out.

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The effect of repeated porcelain firings on corrosion resistance of different dental alloys

  • Tuncdemir, Ali Riza;Karahan, Ismail;Polat, Serdar;Malkoc, Meral Arslan;Dalkiz, Mehmet
    • The Journal of Advanced Prosthodontics
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    • v.5 no.1
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    • pp.44-50
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    • 2013
  • PURPOSE. The aim of this study was to evaluate the effects of repeated porcelain firing process on the corrosion rates of the dental alloys. MATERIALS AND METHODS. Cr-Co, Cr-Ni and Pd-Ag alloys were used for this study. Each metal supported porcelain consisted of 30 specimens of 10 for 7, 9 and 11 firing each. Disc-shaped specimens 10 mm diameter and 3 mm thickness were formed by melting alloys with a propane-oxygen flame and casted with a centrifuge casting machine and then with the porcelain veneer fired onto the metal alloys. Corrosion tests were performed in quintuplicate for each alloy (after repeated porcelain firing) in Fusayama artificial saliva solution (pH = 5) in a low thermal-expansion borosilicate glass cell. Tamhane and Sheffe test was used to compare corrosion differences in the results after repeated firings and among 7, 9 and 11 firing for each alloy. The probability level for statistical significance was set at ${\alpha}$=0.05. RESULTS. The corrosion resistance was higher (30 mV), in case of 7 times firing (Commercial). On the other hand, it was lower in case of 11 times firing (5 mV) (P<.05). Conclusion. Repeated firings decreased corrosion resistance of Pd-Ag, Cr-Co and Cr-Ni alloys. The Pd-Ag alloy exhibited little corrosion in in vitro tests. The Cr-Ni alloy exhibited higher corrosion resistance than Cr-Co alloys in in vitro tests.

Development of Modified Flexibility Ratio - Racking Ratio Relationship of Box Tunnels Subjected to Earthquake Loading Considering Rocking

  • Duhee Park;Van-Quang Nguyen;Gyuphil Lee;Youngsuk Lee
    • Journal of the Korean GEO-environmental Society
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    • v.24 no.2
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    • pp.13-24
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    • 2023
  • Tunnels may undergo a larger or a smaller response compared with the free-field soil. In the pseudo-static procedure, the response of the tunnel is most often characterized by a curve that relates the racking ratio (R) with the flexibility ratio (F), where R represents the ratio of the tunnel response with respect to the free-field vibration and F is the relative stiffness of the tunnel and the surrounding soil. A set of analytical and empirical curves that do not account for the depth and the aspect ratio of the tunnel are typically used in practice. In this study, a series of dynamic analyses are conducted to develop a set of F-Rm relations for use in a frame analysis method. Rm is defined as an adjusted R where the rocking mode of deformation is removed and only the racking deformation is extracted. The numerical model is validated against centrifuge test recordings. The influence of aspect ratio, buried depth of tunnel on results is investigated. The results show that Rm increases with the increase of the buried depth and the aspect ratio. The widely used F-R relations are highlighted to be different compared with the obtained results in this study. Therefore, the updated F-Rm relations with proposed equations are recommended to be used in practice design. The rocking response decreases with either the decrease of the difference of stiffness between surrounding soil and tunnel or the larger aspect ratio of the tunnel section.

A Study on the Dynamic p-y Curves in Soft Clay by 1 g Shaking Table Tests (1g 진동대 실험을 이용한 연약 점성토 지반에서의 동적 p-y 곡선 연구)

  • Han, Jin-Tae;Yoo, Min-Taek;Choi, Jung-In;Kim, Myoung-Mo
    • Journal of the Korean Geotechnical Society
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    • v.26 no.8
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    • pp.67-75
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    • 2010
  • In this study, a series of 1 g shaking table tests were carried out for a single pile in soft clay with various input acceleration amplitudes and frequencies. Based on the results, dynamic p-y curves were drawn and, in turn, the dynamic p-y backbone curve was formed by connecting the peak points, corresponding to the maximum soil resistance, of the dynamic p-y curves. In order to represent the p-y backbone curve numerically, Matlock's p-y formulations for clay was used to find the initial stiffness ($k_{ini}$) and the ultimate capacity ($p_u$) of the clay, both of which are required to formulate the p-y backbone curve as a hyperbolic function. The suggested p-y backbone curve was verified through comparisons with currently available p-y curves as well as other researchers' centrifuge test results and numerical analysis results.

Effect of Osmolality on the Absorption of Ethanol in the Rabbit (삼투질농도가 Ethanol 흡수(吸收)에 미치는 효과)

  • Kim, Shin-Yoon;Kim, Byoung-Guk;Yoo, Kyoung-Mu;Kim, Hyeong-Jin;Park, Jae-Sik;Hwang, Soo-Kwan
    • The Korean Journal of Physiology
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    • v.15 no.1
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    • pp.53-59
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    • 1981
  • In the present study, an effort was directed to elucidate the effect of osmolality on the absorption of ethanol in rabbits. A single dose of 13.67 ml(2. 16 gm ethanol/kg BW) of hypo-, iso-hyphen and hypertonic ethanol per kg BW was administered into the stomach to albino rabbits and the experiment was performed at 30 th, 60 th and 120 th minute. The blood ethanol level was determined by the method of Williams et al, and hematocrit(Hct) was determined by the conventional Hct centrifuge and reader. The results are summarized as follow. The blood ethanol level showed the highest value at 60 min after the ethanol ingestion in the hypo- and isotonic groups, $171.3{\pm}13.3\;mg%$ and $204.5{\pm}23.0\;mg%$, respectively, but in the hypertonic group, the highest value was observed at 120min after the ingestion. The absorption rate of ethanol between 0 to 30 min after the ingestion of hypo- and isotonic ethanol was $88.54{\pm}12.04$ and $94.73{\pm}8.33\;mg/min$, respectively, but a decreased value of $44.72{\pm}6.69\;mg/min$ was noted after hypertonic ethanol ingestion comparing with hypo- and isotonic groups, The Hct value after hypo- and isotonic ethanol ingestion was decreased at 30 min but returned to the control level at 120 min. In contrast with hypo- and isotonic ethanol ingestion, hypertonic ethanol ingestion produced an increase of the Hct value at 30 min and returned to the control level at 120 min. The heart rate was increased but the respiratory rate was decreased after ethanol ingestion regardless of the osmolality.

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Displacement Characteristics of Soft Ground by Embankment Construction (제방축조에 의한 연약지반의 변위특성)

  • Ahn, Kwangkuk;Bae, Wooseok
    • Journal of the Korean GEO-environmental Society
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    • v.8 no.2
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    • pp.29-39
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    • 2007
  • In this study, the centrifugal tests were fulfilled to analyze the displacement characteristics caused by the embankment construction on soft ground. Embankment of height, undrained shear strength and with or without geotextile were selected to evaluate the displacement characteristics of soft ground by embankment. As a result, the replacement section without geotextile showed the parabola shape. The replacement section with geotextile showed the trapezoidal shape which represents the uniform settlements. The replacement angle is increasing nearly lineally with increasing the height of embankment and G-level. The position, where the maximum horizontal displacement occurred, was between $0.24H_0$ and $0.35H_0$ and was at $0.3H_0$ on the average. In the case of with and without geotextile, the relationship between the maximum settlement of ground(S) and maximum horizontal displacement(${\delta}_m$) was ${\delta}_m$ = 0.60S, ${\delta}_m$ = 0.54S, respectively.

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Evaluation of Pullout Capacity of Embedded Suction Anchors in Uniform Clay using Numerical Analysis with ALE(Arbitrary Lagrangian Eulerian) Technique (ALE 기법이 적용된 수치해석을 통한 점토지반에서 석션 매입 앵커의 인발 저항력 평가)

  • Na, SeonHong;Jang, In-Sung;Kwon, OSoon;Lee, Seung-Hyung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.4
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    • pp.2428-2435
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    • 2014
  • Numerical analysis with ALE (Arbitrary Lagrangian Eulerian) Adaptive Meshing technique was performed to evaluate the pullout capacity of the embedded suction anchors (ESA) in uniform clay. The numerical method was verified by the previous study, analytical results based on limit-equilibrium theory and centrifuge tests. The pullout capacity of the ESA under horizontal, vertical, and inclined loading were evaluated, and the effect of initial rotation of the ESA on pullout capacity was also investigated. The analysis results showed that the maximum horizontal capacity was obtained at the mid-point, and the each vertical capacity gave the similar value regardless of the loading points. Furthermore, the inclined capacity was decreased as the load inclination angle increased at the mid-point of the anchor, and almost the same pullout capacity was obtained when the initial rotation angles were below 30 degrees.

Factors Affecting the Recovery of Pilots +Gz Tolerance

  • Park, Myunghwan;Jee, Cheolkyu;Kim, Cheonyoung;Seol, Hyeonju
    • Journal of the Ergonomics Society of Korea
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    • v.36 no.5
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    • pp.535-543
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    • 2017
  • Objective: This study was designed to identify factors affecting pilots' +Gz tolerance recovery from +Gz induced exhaustion. Background: +Gz tolerance of pilots has been considered as a crucial factor to fly the modern high performance fighter aircrafts. However, the factors affecting pilots' G-tolerance recovery from +Gz induced exhaustion have not been examined in the acceleration research community. Method: A centrifuge profile consisting of a high +Gz run for pilot's exhaustion and a low +Gz run for pilot's recovery and another high +Gz runs for pilot's second exhaustion was designed. The subjects' +Gz tolerance recovery ratio was measured by ratio of second high +Gz run time to the first high +Gz run time. The subjects' +Gz tolerance recovery rate was measured by dividing the subjects' +Gz tolerance recovery ratio by the low +Gz run time. The subjects' G-tolerance recovery rate was analyzed with respect to the subjects' personal factors including subjects' anthropometric and physiologic characteristics, flight time, flying aircraft type and so on. Results: The subjects' previous three-month flight hours (r=-0.336, p=0.039), six-month flight hours (r=-0.403, p=0.012) and one-year flight hours (r=-0.329, p= 0.044) correlated with the subjects' G-tolerance recovery rate. Conclusion: The subjects' G-tolerance recovery rate is clearly related to the subjects' previous flight hours. However, the subjects' anthropometric and physiologic characteristics do not show any statistically significant correlation with the subjects' G-tolerance recovery rate. Application: This research provides a safety critical insight to aviation community by identifying the factors to affect the gravity-induced loss of consciousness (GLOC) of pilots.