• Parasites, Hosts and Diseases
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• v.52 no.2
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• pp.143-149
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• 2014

To evaluate the seroprevalence against circumsporozoite protein (CSP) of Plasmodium vivax in sera of Korean patients, the central repeating domain (CRD) of CSP was cloned and analyzed. From the genomic DNA of patient's blood, 2 kinds of CSPs were identified to belong to a VK210 type, which is the dominant repeating of GDRA(D/A)GQPA, and named as PvCSPA and PvCSPB. Recombinantly expressed his-tagged PvCSPA or PvCSPB in Escherichia coli reacted well against sera of patients in western blot, with the detecting rate of 47.9% (58/121), which included 15 cases positive for PvCSPA, 6 cases positive for PvCSPB, and 37 cases for both. The mixture of PvCSPA and PvCSPB was loaded to a rapid diagnostic test kit (RDT) and applied with the same set of patient sera, which resulted in detection rates of 57.0% (69/121). When the protein sequences of PvCSPA were compared with those of P. vivax in endemic regions of India and Uganda, they were compatibly homologous to PvCSPA with minor mutations. These results suggested that the recombinant PvCSPA and PvCSPB loaded RDT may be a milestone in latent diagnosis which has been a hot issue of domestic malaria and important for radical therapy in overlapped infections with P. falciparum in tropical and subtropical areas. During the biological process of malarial infection, exposure of CSP to antigen-antibody reaction up to 57.0% is the first report in Korea.

• Journal of Korean society of Dental Hygiene
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• v.2 no.1
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• pp.43-61
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• 2002

The purpose of this study was focus on development of educational objectives by using oral health education in the elementary school from now on. Based on the results of analysis compared with a model of educational objectives and contents by reflecting the programs for analysis were the oral health education programs of the Ministry of health and social Affairs in Korea, and the programs of two states in u.s. in order to construct goals and contents of oral health education in school. The results in this study can be arranged as follows: First, an analysis on the educational objectives of dental health education program resulted that, from a viewpoint of Bloom's taxonomy of educational objectives, in case of domestic, most learning objectives expressed in the handbook of Ministry of Health and social Welfare were given too much emphasis to the cognitive domain of 37 an items in all. Affective domain were appeared the lack of balance of the development domain. Second, the result of analysis for the programs of two states of America, the cognitive domain were the most abundant of 48 an items in all, and were not significantly different to those of Korea in the form of objectives domain or statement. However a big different was shown that they organized the same objectives domain as a pattern of repeating and deepening at every grade. Third, in this study, 10 educational objectives of dental health education were established, based on the result of analyzing those existing educational programs as central figure. Those are oral cavity, brushing, nutrition of teeth, fluoride, flossing, teeth injury, dental decay, periodontal disease, malocclusion, routine dental visits. And general objectives and specific objectives of each learning area were stated and arranged grade by grade.