• Journal of Microbiology and Biotechnology
• /
• v.17 no.6
• /
• pp.993-1001
• /
• 2007

The technique of serological analysis of antigens by recombinant cDNA expression library (SEREX) uses autologous patient sera as a screening probe to isolate tumor-associated antigens for various tumor types. Isolation of tumor-associated antigens that are specifically reactive with patient sera, but not with normal sera, is important to avoid false-positive and autoimmunogenic antigens for the cancer immunotherapy. Here, we describe a selection methodology to isolate patient sera-specific antigens from a yeast surface-expressed cDNA library constructed from 15 patient lung tissues with non-small cell lung cancer (NSCLC). Several rounds of positive selection using patient sera alone as a screening probe isolated clones exhibiting comparable reactivity with both patient and normal sera. However, the combination of negative selection with allogeneic normal sera to remove antigens reactive with normal sera and subsequent positive selection with patient sera efficiently enriched patient sera-specific antigens. Using the selection methodology described here, we isolated 3 known and 5 unknown proteins, which have not been isolated previously, but and potentially associated with NSCLC.

• 한국정보통신설비학회:학술대회논문집
• /
• 2003.08a
• /
• pp.159-162
• /
• 2003

This paper presents a novel heuristic mechanism, Dynamic-network Adapted Cost selection (DAC-selection), which has higher backup path sharing rate, lower number of blocked channel requests and number of used wavelengths fer reservation of working path and backup path by using unique cost function than that of widely used random selection (R-selection) mechanism and Combined Min-cost selection (CMC-selection) mechanism proposed by Lo, while maintaining 100% restoration capability.

• Journal of Science Education
• /
• v.39 no.2
• /
• pp.278-289
• /
• 2015

The purpose of this study is to analyze selection methods of gifted students. This study focuses on the understanding the characteristics of gifted students in accordance with the selection methods, i.e. the examination selection and automatic promotion by analyzing and comparing the academic emotion, meta-cognition, and self-efficacy between gifted students selected according to the selection methods. Moreover, for the purpose of the effective gifted education, this study aims to arrange a reasonable basis for the discrimination and selection of gifted students. The results of this study were as follows. First, there was no meaningful difference between gifted students selected by an examination and promoted automatically in view of academic emotion, meta-cognition, and self-efficacy of gifted students. It is determined that there is no difference between the effects of selection methods under the condition of the same group of gifted students. Second, regarding the academic emotion of gifted students, there is no significant difference in both the elementary and middle school in case of examination selection. However, in case of the automatic promotion, the academic emotion of gifted students promoted automatically in the gifted education center was higher than that of the gifted students in the gifted class (p < .05). Regarding the meta-cognitive skill, there is no difference in the elementary school between the selection methods. In case of the examination selection in the middle school, the meta-cognitive skill of male students of the gifted education center was higher than that of the female and gifted class students (p < .05). In case of the automatic promotion in the middle school, the meta-cognitive skill of students of the gifted education center was higher than that of students of the gifted class (p < .05). As for the case of self-efficacy, there were no differences between the selection methods. In the automatic promotion, self-efficacy of students of the gifted education center was higher than that of students of the gifted class (p < .05).

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.8
• /
• pp.1073-1082
• /
• 2012

We first sought to clarify the effects of discounted rate, survival rate, and lactation persistency as a component trait of the selection index on net merit, defined as the first five lactation milks and herd life (HL) weighted by 1 and 0.389 (currently used in Japan), respectively, in units of genetic standard deviation. Survival rate increased the relative economic importance of later lactation traits and the first five lactation milk yields during the first 120 months from the start of the breeding scheme. In contrast, reliabilities of the estimated breeding value (EBV) in later lactation traits are lower than those of earlier lactation traits. We then sought to clarify the effects of applying single nucleotide polymorphism (SNP) on net merit to improve the reliability of EBV of later lactation traits to maximize their increased economic importance due to increase in survival rate. Net merit, selection accuracy, and HL increased by adding lactation persistency to the selection index whose component traits were only milk yields. Lactation persistency of the second and (especially) third parities contributed to increasing HL while maintaining the first five lactation milk yields compared with the selection index whose only component traits were milk yields. A selection index comprising the first three lactation milk yields and persistency accounted for 99.4% of net merit derived from a selection index whose components were identical to those for net merit. We consider that the selection index comprising the first three lactation milk yields and persistency is a practical method for increasing lifetime milk yield in the absence of data regarding HL. Applying SNP to the second- and third-lactation traits and HL increased net merit and HL by maximizing the increased economic importance of later lactation traits, reducing the effect of first-lactation milk yield on HL (genetic correlation ($r_G$) = -0.006), and by augmenting the effects of the second- and third-lactation milk yields on HL ($r_G$ = 0.118 and 0.257, respectively).

• Journal of radiological science and technology
• /
• v.22 no.2
• /
• pp.61-68
• /
• 1999

The purpose of this study is to present controversial point and reform measurements by analysing factors haying important effect on selection of upper gastrointestinal study in total health promotion. We examined 200 persons for this study, who visited for upper gastrointestinal study from January to February in 1999. We classified this group into Endoscopy, Upper gastrointestinal series, and sleeping endoscopy. We also investigated standard of satisfaction and factors having effect on selection of each study. As a results, in the motive of selection, Item of 'making accurate observation' and 'without pain' was 39.3% and 34.7%, respectively. In this study, sleeping endoscopy was 45.7%, but on the other side upper gastrointestinal series was low 22.6%(P<0.05). In the standard of preference of study, the man was 55.7% in the endoscopy, and the woman was 61.8% in the upper gastrointestinal series(P<0.05). The standard of preference of upper gastrointestinal series show that it was satisfied on the whole irrespective of sex, dwelling place, age, occupation, and level of education. In the selection of study, one's own will was showed the highest frequency, and family inducement was showed second(P<0.05). Persons over 60% were examined before the same study. Selection of upper gastrointestinal series was 47.9% of person with normal findings, and endoscopy and sleeping endoscopy was over 70% with gastritis, gastric and duodenal(P<0.01). For one's accurate selection of examination, it is important that objective and credible information should be given to a recipient for examination.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.16 no.12
• /
• pp.2575-2580
• /
• 2012

The aim of this paper is to analyze factors for site selection of the cloud data center and to develop spatial data model considering disasters information based on the GIS. In this paper, historical areas of the natural and human disaster are considered to analyze location of the cloud center. The model is developed using ArcGIS S/W tool. The model is applied on Busan city using disaster data from storm and flood, and small administrative district located Kang-Seo-Gu is selected as site selection of the cloud data center of Busan.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.2
• /
• pp.230-234
• /
• 2019

Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.54 no.6
• /
• pp.974-981
• /
• 2021

The aim of this study was to evaluate genetic parameters of growth traits for improving selective breeding in olive flounder Paralichthys olivaceus produced in April 2019. The total length and body weight at 11-, 18-, and 22-months-old were measured for 7,479, 2,831 and 1,904 individuals, respectively. Since 2004, we have been conducting a selective breeding program to improve growth traits in the olive flounder. Data were analyzed using the restricted maximum likelihood method using the multiple traits animal model. The effect of sex and production period showed significant differences in all traits (P<0.05). The heritability of all traits was 0.428-0.520, which is relatively high by measurement month. Therefore, it is considered that individual selection will be more advantageous than family selection. However, to maintain an appropriate degree of inbreeding and genetic diversity for future generations, it is necessary to consider family selection adequately. Results of the correlation analysis between the same traits according to the measurement period indicated that considering production costs such as feed cost, selection at 18-months-old will be advantageous. Olive flounder is a major aquaculture species in Korea, and continuous selective breeding research is essential to improve productivity.

• Journal of Institute of Control, Robotics and Systems
• /
• v.19 no.12
• /
• pp.1081-1088
• /
• 2013

Hyperspectral cameras acquire reflectance values at many different wavelength bands. Dimensions tend to increase because spectral information is stored in each pixel. Several attempts have been made to reduce dimensional problems such as the feature selection using Adaboost and dimension reduction using the Simulated Annealing technique. We propose a novel material detection method that consists of four steps: feature band selection, feature extraction, SVM (Support Vector Machine) learning, and target and specific region detection. It is a combination of the band ratio method and Simulated Annealing algorithm based on detection rate. The experimental results validate the effectiveness of the proposed feature selection and band ratio method.

• Journal of Digital Convergence
• /
• v.16 no.10
• /
• pp.11-26
• /
• 2018

A strategic technology selection for GHG reduction is crucial to secure mitigation means. Especially, a technology selection for a public sector is encouraged to consider integrated perspectives due to various stakeholders under public goals. However, previous studies have mainly focused on technological and economic factors, moreover, consistent criteria have not been applied. This study develops indicators for the GHG reduction technology selection from the public perspective based on delphi method with 22 experts. The result provides valid indicators of technology selection for GHG reduction considering an aspect of technology, economics, environment, policy, society. Specifically, 16 indicators from 5 categories on commercialized technology, and 18 indicators from 5 categories on new technology. We expect that those indicators are useful for a decision-making tool of technology selection. Moreover, provide the basis for the study of judgement criteria to evaluate GHG reduction technology.