• Tuberculosis and Respiratory Diseases
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• v.40 no.2
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• pp.98-103
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• 1993

Background: Inactivation of retinoblastoma gene (Rb) has been observed in a variety of human cancers. Loss of heterozygosity (LOH) of Rb which is a common mode of allelic inactivation of Rb, has been known as a frequent genetic event in small cell lung cancer but it has been detected less frequently in non-small cell lung cancer. To define the role of Rb deletion in lung cancer, we investigated the genomic DNAs of 43 non-small cell lung cancers and 1 small cell lung cancer paired with normal lung tissues obtained by thoracotomy. Methods: The genomic DNAs were obtained by the digestion with proteinase K followed by phenol-chloroform extraction method. The genomic DNAs were digested by restriction endonuclease (EcoRI), separated by agarose gel electrophoresis, transferred to nylon membrane by Southern blot transfer and then hybridized with labelled Rb 1 probe which contains. 1.4 kb sized DNA sequence containing N-terminal portion of Rb. Results: In 26 squamous cell lung cancers, 16 cases were informative after EcoRI digestion and LOH of Rb was found in 10 cases (62.5%). In 17 adenocarcinomas of lung, 11 cases were informative and LOH of Rb was found in five cases (45.4%). The analysis of clinical parameters revealed no significant differences between the two groups with or without LOH of Rb in the aspects of age, sex, degree of differentiation, stage and smoking amount. Conclusions: These results suggest that Rb inactivation is also significantly involved in the molecular pathogenesis of non-small cell lung cancer.

• Journal of Microbiology and Biotechnology
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• v.17 no.8
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• pp.1249-1253
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• 2007

A hyperthermostable endoglucanase from Pyrococcus horikoshii with the capability of hydrolyzing crystalline cellulose was analyzed. A protein engineering study was carried out to obtain a reduced-size mutant. Five amino acid residues at both the N- and C-terminus were found to be removable without any loss of activity or thermal stability. Site-directed mutagenesis was also performed on R102, N200, E201, H297, Y299, E342, and W377, residues possibly involved in the active center or in the recognition and binding of a cellulose substrate. The activity of the resulting mutants was considerably decreased, confirming that the mutated residues were all important for activity. A reduced-size enzyme, as active as the wild-type endoglucanase, was successfully obtained, plus the residues critical for its activity and specificity were confirmed. Consequently, an engineered enzyme with a reduced size was obtained, and the amino acids essential for activity were confirmed by site-directed mutagenesis and comparison with a known three-dimensional structure.

• Journal of Microbiology
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• v.45 no.6
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• pp.547-552
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• 2007

Previously, we generated monoclonal antibodies (MAbs) that bound to the surface of human embryonic stem cells (hESCs) in an attempt to discover new hESC-specific surface markers. In this study, MAb 47-235 (IgG1, ${\kappa}$) was selected for further characterization. The MAb bound to the surface of undifferentiated hESCs but did not bind to mouse ESCs or mouse embryonic fibroblast cells in flow cytometric analysis. The antibody immunoprecipitated a 47 kDa protein from the lysates of cell surface-biotinylated hESCs. Identification of the protein by quadrupole time of flight tandem mass spectrometry revealed that 47-235 binds to Ag 243-5 protein of Mycoplasma arginini. BM-Cyclin treatment of the hESCs that reacted with 47-235 resulted in loss of mycoplasma DNA and the reactivity to 47-235. Nevertheless, the hESCs that were reactive to 47-235 maintained self-renewal and pluripotency and thus could be differentiated into three embryonic germ layers.

• ETRI Journal
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• v.27 no.5
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• pp.643-646
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• 2005

This letter proposes a band-pass filter (BPF) with two transmission zeros based on a combination of parallel coupling and end coupling of half-wave transmission lines. The fabricated BPF exhibited a narrow bandwidth and two transmission zeros near the pass-band due to the end-coupled and shielding waveguide. At the center operation frequency of 60 GHz, the 20 dB bandwidth of the BPF is 1.0 GHz, which is almost 2% of the center operation frequency, and the insertion loss is 3.12 dB. Two transmission zeros reach approximately 40 dB at 58.5 and 62.5 GHz. The simulation results almost agree with the measured results.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.85.1-85.1
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• 2015

We investigate the mass and energy of erupting plasma observed in X-rays and EUV, which is associated with a coronal mass ejection (CME) and an X-class flare. The erupting plasma was observed by both the X-ray telescope (XRT) on Hinode and the Atmospheric Imaging Assembly (AIA) on Solar Dynamic Observatory (SDO). We estimate the emission measures of the erupting plasma using a differential emission measure method. The plasma erupts with a loop-like structure in X-ray and EUV. We estimate the mass of erupting plasma assuming a cylinder structure. In addition, we estimate the radiative loss, thermal conduction, thermal, and kinetic energies of the eruptive hot plasma. We find that the thermal conduction timescale is much shorter than the duration of the eruption. This result implies that additional heating during the eruption may be required to explain the hot plasma observations in X-rays.

• International Journal of Fluid Machinery and Systems
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• v.6 no.2
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• pp.49-55
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• 2013

Water lubricated guide bearing was newly released and has been applied to actual hydro turbines with vertical shaft. As a result, they can have not only high bearing performance but environmental advantages in meeting the demand for reducing river pollution by oil leakage from oil lubricated guide bearing. The PTFE composite guide bearing was tested by experimental equipment operated under conditions similar to those of actual hydro turbines. Circumferential and axial tilting bearing segments help to improve the bearing performance and efficiency due to low friction loss in the bearing system. Furthermore, bearing cooling systems could be eliminated and maintenance periods could be extended, thus the initial investment and operating costs of the hydroelectric power plant are reduced.

• Journal of Sasang Constitutional Medicine
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• v.25 no.4
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• pp.425-431
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• 2013

Objectives The purpose of this case study was to evaluate the effects of Sasang Constitutional diagnosis and treatment of recurrent gastric cancer patient. Methods Nausea and vomiting of recurrent gastric cancer patient was diagnosed Soyangin Emheooyol Pattern based on their Nature & Emotion, physical characteristics, symptoms. He was medicated dokhwaljihwang-tanggami. Results and Conclusions Nausea and vomiting of recurrent gastric cancer patient who was treated with Dokhwaljihwang-tanggami showed improvement in nausea vomiting appetite loss and general condition. This case study describe the effectiveness on Nausea and vomiting of Recurrent Gastric Cancer Patient by using Dokhwaljihwang-tanggami.

• Journal of Korean Dental Science
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• v.9 no.2
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• pp.81-89
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• 2016

Necrotizing periodontal diseases, especially acute necrotizing ulcerative gingivitis (ANUG), it should be noted, occur abruptly and progress rapidly, eventually causing severe soft-tissue and alveolar bone loss. This report presents the cases of two ANUG patients and provides a brief treatment protocol for easy and effective clinical management. After proper diagnosis, sequential treatment with cessation of mechanical brushing, along with a prescription of systemic antibiotics and chlorhexidine as a mouth rinse, scaling, root planing, and supportive periodontal therapy, was utilized. In all cases discussed in this report, there was marked improvement in a few days. ANUG, though an uncommon disease, can be efficiently managed with proper diagnosis and immediate treatment.

• International Journal of Fluid Machinery and Systems
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• v.2 no.1
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• pp.21-30
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• 2009

Slurry wear with sand particles in rivers is a serious problem for pump operation. Therefore, a technique to predict wear volume loss is required for selecting wear resistant materials and determining specifications for the maintenance period. This paper reports a method for predicting the wear depth distribution on the blade of an impeller. Slurry wear tests of an aluminum pump impeller were conducted. Prediction results of wear depth distribution approximately correspond with the results of slurry wear tests. This technique is useful for industrial application.

• Proceedings of the KIEE Conference
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• 1990.11a
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• pp.214-217
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• 1990

The voltage of distribution systems is controlled according to the proper rate of voltage drop and the loss constraints etc.. In modern distribution system it is necessary to supply energy according to the priority of reliabilities of the system. This paper proposes an efficient algorithm of the management of distribution systems considering the system reliabilities and gives a more accurate and economic control of the voltage of distribution systems.