• Journal of Embryo Transfer
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• v.15 no.3
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• pp.247-253
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• 2000

This study was undertaken to optimize enucleation and reconstitution methods for the production of cloned mice by somatic cell nuclear transfer Outbred ICR mouse oocytes at the metapahse- II stage were retrieved from female mice superovulated by PMSG and hCG. In Experiment 1, oocytes were enucleated in medium supplemented with cytochalasin B (CCB) of 3 levels (0, 7.5 or 15 $\mu\textrm{g}$/mL), and higher rate of encleation was obtained at 7.5 and 15 $\mu\textrm{g}$/mL than at $\mu\textrm{g}$/mL. In Experiment 2, oocytes enucleated in 7.5 $\mu\textrm{g}$/mL CCB-containing medium were reconstituted with different types of somatic cell by following methods; 1) cumulus cells by direct cell injection, 2) cumulus cells by electric fusion (1.25 kV/cm, 2 pulses for each 70 $mutextrm{s}$) or 3) STO cells by the electrofusion. Electrofusion of STO cells with enucleated oocytes yielded the greatest (P<0.05) rate of reconstitution without lysis (76%) than any other combinations. Although significant decrease in the rate of somatic cell introduction was found, the electrofusion of cumulus cells yielded better rate of reconstitution than direct injection (0 vs. 18%). In Experiment 3, the duration of electric stimulation for the fusion was changed to either 50 $mutextrm{s}$ or 90 $mutextrm{s}$, but no significant improvement of reconstitution efficacy was obtained. In conclusion, this study showed that ICR mouse oocytes could be used for the production of reconstituted oocytes and a fusion method of 1.25 KV/cm with 2 pulses using 570 cell was the optimal.

• Korean Journal of Community Nutrition
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• v.21 no.6
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• pp.533-544
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• 2016

Objectives: We aimed to examine levels of physical activity, anthropometric features, and health-related quality of life (HRQoL) among Korean breast cancer survivors who reported changes in their diet after diagnosis. Methods: A total of 380 women who had been diagnosed with stage I to III breast cancer and had breast cancer surgery at least six months before the interview were included. Participants provided information on dietary change after diagnosis, post-diagnostic diet, physical activity, anthropometric measures, and HRQoL through face-to-face interview. We assessed HRQoL levels of breast cancer survivors using a validated Korean version of European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Core 30 (EORTC QLQ-C30) and Breast Cancer Module (BR23). We used the logistic regression and generalized linear models to identify the associations of dietary changes in relation with physical activity, anthropometry, and HRQoL. Results: The majority of participants (72.6%) reported that they have changed their diet to a healthier diet after diagnosis. Breast cancer survivors who reported to have change to a healthy diet had higher intakes of vegetables and fruits and lower intakes of red and processed meats, and refined grains than those who did not. Also, survivors with a healthy change in their diet were more likely to engage in physical activity (top vs. bottom tertile: odds ratio [OR], 1.85; 95% confidence interval [95% CI], 1.02-3.36) and have lower body mass index (BMI) (OR, 0.90; 95% CI, 0.82-0.98 for one $kg/m^2$ increment in BMI) compared to those who did not. We found that a healthy change in diet was associated with higher scores of physical functioning (p=0.02) and lower scores of constipation (p=0.04) and diarrhea (p=0.006) compared to those who did not. Conclusions: Healthy changes in diet after breast cancer diagnosis may be associated with lower levels of BMI, and higher levels of physical activity and HRQoL.

• Development and Reproduction
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• v.17 no.4
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• pp.461-467
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• 2013

Nesfatin-1/NUCB2, which is associated with the control of appetite and energy metabolism, was reported for the first time to be expressed in the hypothalamus. However, recent studies have shown that nesfatin-1/NUCB2 was expressed not only in the hypothalamus, but also in various tissues including digestive and reproductive organs. We also demonstrated that nesfatin-1/NUCB2 was expressed in the reproductive organs, pituitary gland, heart, lung, and gastrointestinal tract of the adult mouse. However, little is known about nesfatin-1/NUCB2 expression in fetal and neonatal mice. Therefore, we examined here the distribution of nesfatin-1/NUCB2 in various organs of fetal and neonatal mice and compared them with the distribution in adult mice. As a result of immunohistochemical staining, nesfatin-1/NUCB2 protein was expressed relatively higher in the lung, kidney, heart, and liver compared to other organs in the fetus. Western blot results also showed that nesfatin-1/NUCB2 protein was detected in the lung, kidney, heart, and stomach. Next, we compared the expression levels of nesfatin-1/NUCB2 mRNA in the fetus and neonate with the expression levels in both male and female adult mice. The expression levels in heart, lung, stomach, and kidney were higher compared with other organs in fetal and neonatal mice and in both male and female adult mice. Interestingly, the expression of nesfatin-1/NUCB2 mRNA in the kidney was dramatically increased in male and female adult mice compared to fetal and neonatal mice. These results indicate that nesfatin-1/NUCB2 may regulate the development and physiological function of mouse organs. In the future, we need more study on the function of nesfatin-1/NUCB2, which is highly expressed in the heart, lung, and kidney during mouse development.

• The korean journal of orthodontics
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• v.27 no.2
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• pp.259-272
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• 1997

This study was performed to locate the anteroposterior position of the center of resistance of upper anterior teeth when intrusive forces are acted on them by applying segmented arch mechanics. Three-dimensional finite element model of upper six anterior teeth, periodontal ligament and alveolar bone was constructed The locations of the center of resistance were compared according to the three variables, which are number of teeth contained in anterior segment, axial inclination of anterior teeth, and degree of alveolar bone loss. The following conclusions were drawn from this study; 1. When the axial inclination and alveolar bone height were normal, the locations of center of resistance of anterior segment according to the number of teeth contained were as follows; 1). In 2 teeth segment, the center of resistance was located in the distal area of lateral incisor bracket 2) In 4 teeth segment, the center of resistance was located in the distal 2/3 of the distance between the brackets of lateral incisor and canine. 3) In 6 teeth segment, the center of resistance was located in 3mm distal of canine bracket, which is interproxirnal area. between canine and 1st premolar. 4) As the number of teeth contained in anterior segment increased, the center of resistance shifted to the distal side. 2. As the labial inclination of incisors increased, the center of resistance shifted to the distal side. 3. As the alveolar bone loss increased, the center of resistance shifted to the distal side.

• Korean Journal of Radiology
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• v.23 no.11
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• pp.1078-1088
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• 2022

Objective: To develop and validate a model using radiomics features from apparent diffusion coefficient (ADC) map to diagnose local tumor recurrence in head and neck squamous cell carcinoma (HNSCC). Materials and Methods: This retrospective study included 285 patients (mean age ± standard deviation, 62 ± 12 years; 220 male, 77.2%), including 215 for training (n = 161) and internal validation (n = 54) and 70 others for external validation, with newly developed contrast-enhancing lesions at the primary cancer site on the surveillance MRI following definitive treatment of HNSCC between January 2014 and October 2019. Of the 215 and 70 patients, 127 and 34, respectively, had local tumor recurrence. Radiomics models using radiomics scores were created separately for T2-weighted imaging (T2WI), contrast-enhanced T1-weighted imaging (CE-T1WI), and ADC maps using non-zero coefficients from the least absolute shrinkage and selection operator in the training set. Receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic performance of each radiomics score and known clinical parameter (age, sex, and clinical stage) in the internal and external validation sets. Results: Five radiomics features from T2WI, six from CE-T1WI, and nine from ADC maps were selected and used to develop the respective radiomics models. The area under ROC curve (AUROC) of ADC radiomics score was 0.76 (95% confidence interval [CI], 0.62-0.89) and 0.77 (95% CI, 0.65-0.88) in the internal and external validation sets, respectively. These were significantly higher than the AUROC values of T2WI (0.53 [95% CI, 0.40-0.67], p = 0.006), CE-T1WI (0.53 [95% CI, 0.40-0.67], p = 0.012), and clinical parameters (0.53 [95% CI, 0.39-0.67], p = 0.021) in the external validation set. Conclusion: The radiomics model using ADC maps exhibited higher diagnostic performance than those of the radiomics models using T2WI or CE-T1WI and clinical parameters in the diagnosis of local tumor recurrence in HNSCC following definitive treatment.

• Bulletin of the Korean Chemical Society
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• v.33 no.10
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• pp.3279-3284
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• 2012

Poly(benzimidazole-co-benzoxazole)s (PBI-co-PBO) are synthesized by polycondensation reaction with 3,3'-diaminobenzidine, terephthalic acid and 3,3'-dihydroxybenzidine or 4,6-diaminoresorcinol in polyphosphoric acid (PPA). All polymer membranes are prepared by the direct casting method (in-situ fabrication). The introduction of benzoxazole units (BO units) into a polymer backbone lowers the basic property and $H_3PO_4$ doping level of the copolymer membranes, resulting in the improvement of mechanical strength. The proton conductivity of $H_3PO_4$ doped PBI-co-PBO membranes decrease as a result of adding amounts of BO units. The maximum tensile strength reaches 4.1 MPa with a 10% molar ratio of BO units in the copolymer. As a result, the $H_3PO_4$ doped PBI-co-PBO membranes could be utilized as alternative proton exchange membranes in high temperature polymer electrolyte fuel cells.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.64 no.1
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• pp.171-175
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• 2015

In this paper, because of global environmental problems such as energy shortage and abnormal climate, green energy development and energy saving technology development is being magnified. Heat insulation, the most basic and traditional energy saving method, is highly expected. Especially, simple and advanced heat insulation technique based on Nano material is promising future technology. The railway system also requires heat insulation. Especially, traditional heat insulator such as glass wool has been adopted frequently to rolling stock. The heat insulator is also adopted in general railway service buildings. Take account of cost-effective heat blocking performance, the heat insulator shall be adopted in diverse fields such as electric power, railroad, signal and communication. The only and direct solution for this problem is installing heat insulator with outstanding endurance, corrosion resistance and heat resistance to block outer heat Upgrading heat specification of equipment can be a solution, but since price and maintenance cost rise severely, this plan might be incongruous. In this research, energy saving effect of ultrathin heat insulator film was demonstrated by installing the film on roof of electrical room.

• Proceedings of the KSRS Conference
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• 2007.10a
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• pp.556-559
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• 2007

The Urban Environmental Quality (UEQ) indicates a complex and various parameters resulting from both human and natural factors in an urban area. Vegetation, climate, air quality, and the urban infrastructure may interact to produce effects in an urban area. There are relationships among air pollution, vegetation, and degrading environmental the urban heat island (UHI) effect. This study investigates the application of multi-spectral remote sensing data from the Landsat ETM and TM sensors for the mapping of air quality and UHI intensity in Seoul from 2000 to 2006 in fine resolution (30m) using the emissivity-fusion method. The Haze Optimized Transform (HOT) correction approach has been adopted for atmospheric correction on all bands except thermal band. The general UHI values (${\Delta}(T_{urban}-T_{rural})$) are 8.45 (2000), 9.14 (2001), 8.61 (2002), and $8.41^{\circ}C$ (2006), respectively. Although the UHI values are similar during these years, the spatial coverage of "hot" surface temperature (>$24^{\circ}C$) significantly increased from 2000 to 2006 due to the rapid urban development. Furthermore, high correlations between vegetation index and land surface temperature were achieved with a correlation coefficients of 0.85 (2000), 0.81 (2001), 0.84(2002), and 0.89 (2006), respectively. Air quality is shown to be an important factor in the spatial variation of UEQ. Based on the quantifiable fine resolution satellite image parameters, UEQ can promote the understanding of the complex and dynamic factors controlling urban environment.

• Tunnel and Underground Space
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• v.29 no.2
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• pp.89-107
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• 2019

Rock joints have an extremely important role in analyzing the mechanical stability and hydraulic characteristics of rock mass structures. Most rock joint parameters are generally indicated as a distribution by statistical techniques. In this research, calculation technique of Joint Center Volume (JCV) is analyzed, which is required for estimating the size distribution having the largest uncertainty among the joint parameters, then a new technique is proposed which is applicable regardless of the shape of survey window. The existing theoretical JCV calculation technique can be applied only to the plane window, and the complete enumeration techniques show the limitations in joint trace type and analysis time. This research aims to overcome the limitations in survey window shape and joint trace type through calculating JCV by using Monte Carlo simulation. The applicability of proposed technique is validated through the estimation results at non-planar survey windows such as curved surface and tunnel surface.

• Journal of Biomedical Engineering Research
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• v.41 no.6
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• pp.219-227
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• 2020

In this study, we tried to quantify the actual cross-sectional area inside the bore when scanning by the MRI system with various bore sizes. To this end, a comparative analysis was conducted by both of blueprint of each MRI equipment and actual measurement in the field. As a result of analysis, ACSA(Actual Cross-Sectional Area) in Ingenia CX, Elition X, uMR 780, Omega, Vida, Lumina, Architect, Premier is recorded as 171230, 232150, 242100, 309332, 230760, 230760, 229380 and 235990 ㎟, respectively ACSA% was 60.6, 60.3, 73.0, 70.0, 60.0, 60.0, 59.6, and 61,3%. In addition, DTB (Distance from Table top to Bore top) recorded 400, 407, 445, 495, 405, 405, 405, 403, and 412 mm. Through this study, it was confirmed that there is a difference between the bore size according to each MRI system and the actual cross-sectional area during MRI scanning. Accordingly, if we consider the internal actual area just not bore size at the clinical site, useful diagnostic images can be obtained in the end with better convenience.