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Optimization of Growth Medium Composition for Overproduction of Bacillus licheniformis Amylase in Recombinant Escherichia coli (Bacillus licheniformis amylase(BLMA)의 생산성 향상을 취한 재조합 대장균의 배지 최적화)

  • Nam, Seung-Hun;Lee, Woo-Jong;Byun, Tae-Gang;Seo, Jin-Ho;Park, Kwan-Hwa
    • Korean Journal of Food Science and Technology
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    • v.26 no.4
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    • pp.411-416
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    • 1994
  • The research is concerned with optimization of growth medium composition in an attempt to improve the product yield of Bacillus licheniformis amylase (BLMA) in recombinant E. coli containing the BLMA gene. BLMA has the catalytic activity of producing branched oligosaccharides from starch. The medium optimization was performed in flask cultures based on the Box and Wilson method. The optimized medium is composed of tryptone 18.0 g/l, yeast extract 22.4 g/l, NaCl 5.3 g/l and glucose 2.1 g/l. In a jar fermenter culture with the conventional LB medium, the recombinant E. coli yielded 1.39 g/l of final dry cell mass and 5.11 U/ml of enzyme activity. In the optimized medium, however, the final cell mass was increased to 6.01 g/l and the enzyme activity to 23.2 U/ml. Medium optimization improved cell mass by 4.3 times and enzyme activity by 4.5 times. Such an increase in enzyme activity is mainly due to an enhancement of cell mass.

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Ultrasonographic Indeterminate Lymph Nodes in Preoperative Thyroid Cancer Patients: Malignancy Risk and Ultrasonographic Findings Predictive of Malignancy

  • Roh-Eul Yoo;Ji-hoon Kim;Jeong Mo Bae;Inpyeong Hwang;Koung Mi Kang;Tae Jin Yun;Seung Hong Choi;Chul-Ho Sohn;Jung Hyo Rhim;Sun-Won Park
    • Korean Journal of Radiology
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    • v.21 no.5
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    • pp.598-604
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    • 2020
  • Objective: Proper management of lymph nodes (LNs) with ultrasonographic (US) indeterminate features in thyroid cancer patients remains elusive. We aimed to evaluate the malignancy risk and US findings predictive of malignancy for US indeterminate LNs in preoperative thyroid cancer patients through node-by-node correlation. Materials and Methods: A total of 348 LNs in 284 thyroid cancer patients, who underwent fine-needle aspiration or core-needle biopsy between December 2006 and June 2015, were included. We determined the malignancy risks for US probably benign, indeterminate, and suspicious categories. For US indeterminate LNs, which had neither echogenic hilum nor hilar vascularity in the absence of any suspicious finding, US findings were compared between benign and metastatic LNs using Mann-Whitney U test and Fisher's exact test. Results: US imaging diagnoses were probably benign in 20.7% (n = 72) cases, indeterminate in 23.6% (n = 82), and suspicious in 55.7% (n = 194). Malignancy risk of US indeterminate LNs (19.5% [16/82]) differed from those of the US probably benign (2.8% [2/72]) (p = 0.002) and US suspicious LNs (78.4% [152/194]) (p < 0.001). Among US indeterminate LNs, there were no significant differences in short, long, and long-to-short diameter (L/S) ratios between benign and metastatic LNs (3.9 vs. 3.8 mm, p = 0.619; 7.3 vs. 7.3 mm, p = 0.590; 1.9 vs. 1.9, p = 0.652). Conclusion: US indeterminate LNs were frequently encountered during preoperative evaluation and had intermediate malignancy risk. Given the lack of discriminative power of size criteria and L/S ratio, clinical factors such as surgical strategy and node size should be considered for proper triage of US indeterminate LNs in thyroid cancer.

A New Record of Siriella trispina (Crustacea, Mysida, Mysidae) from the Southern Coast of Korea

  • Lee, Hyemin;Kim, Mijin;Song, Sung Joon;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • v.33 no.4
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    • pp.267-273
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    • 2017
  • The opossum shrimp, Siriella Dana, 1850 belongs to the family Mysidae Haworth, 1825. It is the genus of an enormous number of species with frequent morphological variations comprising 84 valid species to date. Among the species, Siriella trispina Ii, 1964 has been recorded from the Yellow Sea and the East China Sea is reported from Korean water for the first time. Siriella trispina is characterized by its exceedingly elongated 5th pair of thoracopods, the spines on the inner ventral margin of the uropodal endopod armed with secondary spinules, and 4-5 pairs of spines on the base of the telson. In the present study, we describe Korean specimens of the species in detail with photographs and illustrations. Siriella trispina is the 8th Siriella species found in Korean fauna.

ANALYSIS OF THE FIXED BED REACTOR FOR DME SYNTHESIS

  • Song, Dae-Sung;Ahn, Sung-Joon;Cho, Won-Jun;Park, Dal-Keun;Yoon, En-Sup
    • 한국가스학회:학술대회논문집
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    • 2007.04a
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    • pp.27-32
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    • 2007
  • Dimethyl Ether (DME, $CH_3OCH_3$) is the simplest ether and is considered as one of the leading candidates in the quest for a substitute fur petroleum-based fuels. In this work, we analyzed the one-step synthesis of DME in a shell and tube type fixed bed reactor and carried out a simulation with a one-dimensional, steady state model of a heterogeneous catalyst bed, while taking into consideration the heat and mass transfer between the catalyst pellets and reactants gas and the effectiveness factor of the catalysts, together with the reactor cooling through the reactor tube wall. The reactor simulation was carried out under steady state condition and we compared the simulation results with the experimental data obtained from operations of a pilot-scale reactor and found good agreement between them.

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Diagnostic Imaging and Treatment of Portoazygous Shunt in a Dog (개에서 문맥홀정맥단락의 진단 영상 및 치료 1 례)

  • Jung, Joo-Hyun;Kwan, Do-Hyoung;Chang, Jin-Hwa;Yoon, Jung-Hee;Choi, Min-Cheol
    • Journal of Veterinary Clinics
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    • v.26 no.1
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    • pp.86-90
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    • 2009
  • An intact female, 5-year old, Shihtzu, weighing 3.2 kg with a history of depression, hypersalivation, dullness, and ataxia for 3 days was referred. Radiographic findings included microhepatica, bilateral renal calculi, and a cystic calculus. Ultrasonography showed microhepatica, decreased visibility of portal veins in the liver, bilateral renal calculi, a cystic calculus, and an abnormal tortuous dilated vessel in the craniodorsal abdomen. A single congenital extrahepatic portoazygous shunt was confirmed through operative mesenteric portography. The patient received surgery using an ameroid constrictor ring and recovered well.

Feasibility Study of Gait Recognition Using Points in Three-Dimensional Space

  • Kim, Minsung;Kim, Mingon;Park, Sumin;Kwon, Junghoon;Park, Jaeheung
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.13 no.2
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    • pp.124-132
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    • 2013
  • This study investigated the feasibility of gait recognition using points on the body in three-dimensional (3D) space based on comparisons of four different feature vectors. To obtain the point trajectories on the body in 3D, gait motion data were captured from 10 participants using a 3D motion capture system, and four shoes with different heel heights were used to study the effects of heel height on gait recognition. Finally, the recognition rates were compared using four methods and different heel heights.

Modeling Embryonic Development in Drosophila by Evolutionary Learning of Dynamical System (동역학 시스템의 진화적 학습에 의한 초파리 발생과정 모델링)

  • Rhee Je-Keun;Nam Jin-Wu;Joung Je-Gun;Zhang Byoung-Tak
    • Proceedings of the Korean Information Science Society Conference
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    • 2005.11b
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    • pp.280-282
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    • 2005
  • 초파리 초기 발생과정은 gap 유전자, pair-rule 유전자, polarity 유전자의 세 가지 유전자 그룹에 의해서 조직화 된다. Gap 유전자들에 의해 pair-rules 유전자들의 발현이 조절되며, 이들에 의해 결국 polarity 유전자들의 발현을 조절함으로써, 정확한 위치에서 각 기관의 형성을 유도한다. 특히 분열 14단계에서는 pair-rule 유전자 중의 하나인 eve 유전자의 발현이 조절되는데, eve 유전자는 배아의 분할의 줄무늬를 형성시키는 유전자에 해당된다. 본 논문에서는 eve 유전자의 발현조절자인 hunchback, giant, kruppel, bicoid의 gap 유전자들로 구성된 조절 네트워크를 S-system을 이용하여 모델링하였다. 이를 통해 각 유전자들의 발현 데이터로부터 파라미터들을 진화 연산을 통해 예측하고, 각 유전자들의 발현에 대한 시뮬레이션 결과를 보여준다. 예측된 결과와 실제 데이터의 비교는 전체적으로 패턴이 서로 유사함을 보여주고 있다.

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Referred Pain in Right Arm from Abdominal Wall Pseudoaneurysm

  • Park, Soo Young;Ahn, Seon Kyoung;Kim, Hye Young;Shin, Ji Yeon;Min, Sangil
    • The Korean Journal of Pain
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    • v.26 no.2
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    • pp.191-194
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    • 2013
  • Pseudoaneurysm of the abdominal wall is a possible but very rare clinical entity. It is a known complication of surgery, trauma, or arterial puncture, but it is rarely spontaneous. Even though it can usually present with a wide range of local symptoms, it can cause referred pain via spinal cord, which is cross-excited with afferent sympathetic nervous system. We report a case of right arm pain which was referred from a small abdominal pseudoaneurysm like a referred pain from gall bladder. This rare entity should be considered in the differential for pain management in case that the pain does not resolve with medication or interventional pain management.

Application of porcine small intestinal submucosa (Vetrix BioSIS®) for recurrent corneal sequestrum in an American shorthair cat

  • Kim, Youngsam;Kang, Seonmi;Nam, Sunhwa;Yun, Seongjin;Seo, Kangmoon
    • Korean Journal of Veterinary Research
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    • v.60 no.4
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    • pp.229-232
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    • 2020
  • A 15-year-old, spayed, female American shorthair cat presented with recurrent corneal sequestrum in the right eye. The cat had undergone superficial keratectomy twice for corneal sequestrum treatment 5 and 11 months previously. Two layers of porcine small intestinal submucosa (SIS; Vetrix BioSIS®) were applied to the surgical corneal defect after keratectomy. Thereafter, clinical signs, such as lacrimation, blepharospasm, and corneal ulcer, disappeared 50 days postoperatively. Moreover, the application of SIS with keratectomy prevented recurrence until 651 days after surgery. SIS could be applied as an additional bioscaffold for surgical repair of corneal sequestrum recurring after superficial keratectomy alone in cats.

Effect of the Extract of Cultured Ginseng Roots on Hyperlipidemia in Rats

  • Yi, Eun-Jeong;Hailin, Zhao;Li, Da-Wei;Paek, Kee-Yeoup;Kim, Jong-Hoon;Kim, Yeong-Shik
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.263.1-263.1
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    • 2002
  • We have investigated the effect of MeOH extract of cultured ginseng roots on hyperlipidemic rats induced by fat-rich diet. We also analyzed and compared ginsenosides of cultured ginsengs by HPLC. After oral administration of the extract to hyperlipidemic rats for four weeks, the variables including body weight, cholesterol, HDL, LDL, and triglyceride levels in serum were measured. One of the cultured ginseng roots (CBN3) decreased cholesterol and LDL -cholesterol and increased HDL-cholesterol levels in serum.. (omitted)

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