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A Study on the Provision of TPN for Hospitalized Patients (입원환자의 TPN 공급현황에 대한 연구)

  • Gang, Eun-Hui;Kim, Mi-Gyeong;Gang, Sin-Suk
    • Journal of the Korean Dietetic Association
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    • v.8 no.1
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    • pp.26-32
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    • 2002
  • In Korea, implementation of nutrition support guidelines has been limited due to strict health insurance reimbursement policies as well as the lack of consensus on the best approach to TPN management. We examined the impact of TPN provision to hospitalized patients where NST (nutrition support team ) consultations were not requested by their primary physicians. The study showed the followings : 1. The median dutation of TPN provision was 8 days, but many patients were on TPN for less than 1 week. 2. The intake of energy and protein were less than the patient's requirements 3. Lipid emulsion was not provided to the most TPN patients. In conclusion, the role of NST should be expanded and studies are needed not only on TPN formulations which are suitable to Koreans but also on the cost-effectiveness of NST activities. TPN policies and protocols should be established based on the needs of each hospital.

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Ionic Recognition with Quinone-Derivatized Calixarenes in Solution and at Self-Assembled Monlayers

  • Kim Hasuck;Kang Sun Kil;Chung Taek Dong
    • Journal of the Korean Electrochemical Society
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    • v.3 no.2
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    • pp.69-71
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    • 2000
  • Redox-active calix[4]arenes with carboxylic acid and disulfide groups were prepared and spontaneous deposition on silver and gold surfaces was observed. Owing to their unusual structure, the calix[4]arenes exhibit selective affinity fur alkaline earth metal ions in aqueous media. When annular ionophores are immobilized on the surface, voltammetric and spectroscopic studies show the entrapment of metal ions. Furthermore, it was possible to reversibly capture and remove the ions using strong chelating agents such as ethylenediaminetetraacetic acid (EDTA).

Purification and Characterization of Protein Phosphatase 2C from Rat Liver

  • Oh, Joung-Sook;Hwang, In-Seong;Choi, Myung-Un
    • BMB Reports
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    • v.30 no.3
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    • pp.222-228
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    • 1997
  • Protein phosphatase 2C (PP2C) is one of the four major serine/threonine phosphatases which is dependent on $Mg^{2+}$ for its activity. PP2C was purified from rat liver cytosol and its characteristics were investigated. The substrate employed for routine assay was $[^{32}P]casein$ phosphorylated by PKA. The purification process involved DEAE chromatography, ammonium sulfate fractionation, phenyl sepharose chromatography, sephacryl 5-200 gel filtration, and histone agarose chromatography. The SDS-PAGE of PP2C showed one major single protein band at a position corresponding to a molecular mass of 43 kd and the purification fold was 637. The enzyme showed a pH optimum of 8 and $K_M$ value was $1.9\;{\mu}M$. However, when the substrate was changed to $[^{32}P]histone$, the pH optimum was shifted to 7 and $K_M$ value was $2.3\;{\mu}M.\;Mg^{2+}$ was essential to the enzyme activity and okadaic acid did not exert any inhibitory effect on the enzyme. To examine residue in the active site of PP2C effects of some protein-modifying reagents were tested.

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Regulatory Characteristics of the Vibrio vulnificus putAP Operon Encoding Proline Dehydrogenase and Proline Permease

  • Lee Jeong-Hyun;Jeong So-Young;Choi Sang-Ho
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1285-1291
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    • 2006
  • The proline utilization (put) operon of Vibrio vulnificus consists of the putAP genes encoding a proline dehydrogenase and proline permease. The result of put-lux transcriptional fusion analysis suggests that the V vulnificus putAP operon is not autoregulated by the PutA protein. A putR null mutation decreased proline dehydrogenase activity and the level of the put transcripts, indicating that transcription of putAP is under the positive control of PutR. The deduced amino acid sequence of the putR was similar to those reported from other bacteria with high levels of identity. Chromatin IP and GST pull-down assays revealed that PutR specifically binds to the putAP promoter region in vivo, and interacts with CRP in vitro. Taken together, the results suggested that PutR exerts its effect on putAP expression by directly interacting with CRP bound to the upstream region of P$_{put}$.

The optical afterglow of GRB 180205A

  • Paek, Gregory SungHak;Im, Myungshin;Choi, Changsu
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.59.1-59.1
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    • 2018
  • On 2018 February 5 a gamma ray burst with trigger time 04:25:29.3 UT was detected by Swift BAT and this event was named GRB 180205A. We observed the optical afterglow of GRB 180205A starting from about 1 hour after the burst until February 22 in the optical bands with the 1m telescope of Deokheung Optical Astronomy Observatory (DOAO), the 1m telescope at Mt. Lemmon Optical Astronomy Observatory(LOAO) and the 0.8m and 0.25m telescopes at McDonald Observatory. According to the fireball model, which is a well-accepted and conventional model for the afterglow of the GRB, the mechanism of the afterglow is that the expanding external blast wave of the GRB successively collides with the ambient medium and loses its energy, and as a result emits radiation at wavelengths longer than gamma rays. Here we present optical photometry and light curve of the afterglow in the R band and analyze it to characterize GRB 180205A.

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Isolation of the Regulator Gene Responsible for Overproduction of Catalase A in $H_2O$$_2$-resistant Mutant of Streptomyces coelicolor

  • Hahn, Ji-Sook;Oh, So-Young;Keith F. Chater;Roe, Jung-Hye
    • Journal of Microbiology
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    • v.38 no.1
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    • pp.18-23
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    • 2000
  • Streptomyces coelicolor produces three kinds of catalases to cope with oxidative stress and to allow normal differentiation. Catalase A is the major vegetative catalase which functions in removing hydrogen peroxide generated during the process of aerobic metabolism. To understand the regulatory mechanism of response against oxidative stress, hydrogen peroxide-resistant mutant (HR4O) was isolated from S. coelicolor J1501 following UV mutagenesis. The mutant overproduced catalase A more than 50-fo1d compared with the wild type. The mutation locus catRI was mapped closed to the mthB2 locus by genetic crossings. An ordered cosmid library of S. coelicolor encompassing the mthB2 locus was used to isolate the regulator gene (catR) which represses catalase overproduction when introduced into HR4O. A candidate catR gene was found to encode a Fur-like protein of 138 amino acids (15319 Da).

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White Organic Light-emitting Diodes using the Tandem Structure Incorporating with Organic p/n Junction

  • Lee, Hyun-Koo;Kwon, Do-Sung;Lee, Chang-Hee
    • Journal of Information Display
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    • v.8 no.2
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    • pp.20-24
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    • 2007
  • Efficient white organic light-emitting diodes are fabricated with the blue and red electroluminescent (EL) units electrically connected in a stacked tandem structure by using a transparent doped organic p/n junction. The blue and red EL units consist of the light-emitting layer of 1,4-bis(2,2-diphenyl vinyl)benzene (DPVBi) and 4-dicyanomethylene-2-methyl-6-[2-(2,3,6,7-tetrahydro-1H,5H-benzo[i,j] quinolizin-8-yl)vinyl]-4H-pyran) (DCM2) doped tris(8-hydroxyquinoline) aluminum $(Alq_3)$, respectively. The organic p-n junction consists of ${\alpha}-NPD$ doped with $FeCl_3$ (15 % by weight ratio) and $Alq_3$ doped with Li (10 %). The EL spectra exhibit two peaks at 448 and 606 nm, resulting in white light-emission with the Commission Internationale d'Eclairage (CIE) chromaticity coordinates of (0.36, 0.24). The tandem device shows the quantum efficiency of about 2.2 % at a luminance of 100 $cd/m^2$, higher than individual blue and red EL devices.

A Triple-Band Transceiver Module for 2.3/2.5/3.5 GHz Mobile WiMAX Applications

  • Jang, Yeon-Su;Kang, Sung-Chan;Kim, Young-Eil;Lee, Jong-Ryul;Yi, Jae-Hoon;Chun, Kuk-Jin
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.11 no.4
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    • pp.295-301
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    • 2011
  • A triple-band transceiver module for 2.3/2.5/3.5 GHz mobile WiMAX, IEEE 802.16e, applications is introduced. The suggested transceiver module consists of RFIC, reconfigurable/multi-resonance MIMO antenna, embedded PCB, mobile WiMAX base band, memory and channel selection front-end module. The RFIC is fabricated in $0.13{\mu}m$ RF CMOS process and has 3.5 dB noise figure(NF) of receiver and 1 dBm maximum power of transmitter with 68-pin QFN package, $8{\times}8\;mm^2$ area. The area reduction of transceiver module is achieved by using embedded PCB which decreases area by 9% of the area of transceiver module with normal PCB. The developed triple-band mobile WiMAX transceiver module is tested by performing radio conformance test(RCT) and measuring carrier to interference plus noise ratio (CINR) and received signal strength indication (RSSI) in each 2.3/2.5/3.5 GHz frequency.

Expression, Purification, and Crystallization of D-Psicose 3-Epimerase from Agrobacterium tumefaciens

  • Kim Kwang-Soo;Kim Hye-Jung;Oh Deok-Kun;Cheong Jong-Joo;Rhee Sang-Kee
    • Journal of Microbiology and Biotechnology
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    • v.16 no.4
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    • pp.647-650
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    • 2006
  • D-Psicose 3-epimerase (DPE) catalyzes the interconversion of D-fructose to D-psicose by epimerizing the carbon-3 position. The DPE from Agrobacterium tumefaciens was cloned and expressed in Escherichia coli. The expressed enzyme was purified by affinity chromatography on an IMAC, gel filtration chromatography on a Sephacryl S-300 HR, and anion-exchange chromatography on a RESOURCE Q. The molecular mass of the purified enzyme was estimated to be about 135 kDa by Superdex 200 gel filtration chromatography, corresponding to a homotetramer. The enzyme produced crystals suitable for X-ray diffraction to a $2.0{\AA}$ resolution at 100 K. The crystals were found to belong to the orthorhombic space group $P2_12_12_1$, with unit-cell parameters a=102.4, b=113.0, and $c=131.8{\AA}$. In addition, the calculated packing parameter $(V_m)$ was $2.79{\AA}^3/Da$, the solvent content was 55.92%, and an asymmetric unit consisted of four monomers.

Synthesis of a Triblock Copolymer Containing a Diacetylene Group and Its Use for Preparation of Carbon Nanodots

  • Kim, Beom-Jin;Oh, Dong-Kung;Chang, Ji-Young
    • Macromolecular Research
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    • v.16 no.2
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    • pp.103-107
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    • 2008
  • Carbon nanodots were prepared by the pyrolysis of a triblock copolymer. The triblock copolymer, poly(methyl methacrylate)-b-polystyrene-b-poly(methyl methacrylate) was synthesized by atom transfer radical polymerization using an initiator containing a diacetylene group. A polymer thin film on a mica substrate was prepared by spin-casting at 2,000 rpm from a 0.5 wt% toluene solution of the triblock copolymer. After drying, the cast film was vacuum-annealed for 48 h at $160^{\circ}C$. The annealed film formed a spherical morphology of polystyrene domains with a diameter of approximately 30 nm. The film was exposed to UV irradiation to induce a cross-linking reaction between diacetylene groups. In the subsequent pyrolysis at $800^{\circ}C$, the cross-linked polystyrene spheres were carbonized and the poly(methyl methacrylate) matrix was eliminated, resulting in carbon nanodots deposited on a substrate with a diameter of approximately 5 mn.