• 한국식품영양과학회지
• /
• 제14권2호
• /
• pp.157-163
• /
• 1985

Various cell wall polysaccharides and related enzyme activities in hot pepper fruit were determined at different stages of maturity. The uronic acid content of cell walls decreased between immature green and turning stage fruit and then increased by red ripe stage. In contrast, cellulose content of cell walls changed only a little during ripening. Total neutal sugar content of cell wall material decreased 50% and galactose content of the walls decreased about 80% by the turning stage. Polygalacturonase and ${\beta}-galactosidase$ activities, as well as total hemicellulose from isolated cell walls of ripening hot pepper fruit were studied using gel filtration chromatography. Polygalacturonase activity was not detectable but 5 isozymes of ${\beta}-galactosidase$ were resolved. The activities of the enzymes were relatively high and gel filtration showed that they differed in molecular weight. Hemicellulose content decreased during ripening and softening. The molecular weight profiles shifted from high molecular weight to low molecular weight polymers during ripening. The changes in cell walls that may be associated with fruit softening involve the alteration of hemicellulose prior to the degradation of wall-bound uronic acid. It is suggested that the decrease in cell wall galactose involved changes in turnover of new cell wall components.

• 한국식품영양과학회지
• /
• 제27권6호
• /
• pp.1041-1046
• /
• 1998

This study was carried out to investigate the changes and composition of the non-cellulosic neutral sugars in cell wall of soybean sprouts during growth. The composition of non-cellulosic neutral sugars in cell of soybean sprouts was rhamnose, fucose, xylose, arabinose, mannose, galactose and glucose. The galactose content of cell wall was higher than other non-cellulosic neutral sugars, and was remarkably decreased during growth. The major non-cellulosic sugars of pectic substances were rhamnose, arabinose, and galactose. The arabinose content of pectic substance was increased in cotyledon and hypocotyl during growth. The contents of non-cellulosic neutral sugars were decreased in hypocotyl during growth. The galactose content of pectic substance was higher in cotyledon than those in hypocotyl, and was increased in cotyledon. The content of rhamnose was higher in ionically associated pectic substance than that in covalently bounded pectic substance. The major non-cellulosic neutral sugars of hemicellulose were glucose, rhamnose, arabinose and galactose. The galactose of hemicellulose was decreased remarkably during growth.

• Applied Biological Chemistry
• /
• 제35권6호
• /
• pp.507-512
• /
• 1992

대두(Glycine max)의 ${\beta}$-1,3-glucanase를 분리동정하고 benzyladenine(BA)이 이 효소의 세포내 함량과 활동도에 미치는 영향을 조사하였다. 또 세포벽의 callose함량과 protoplast의 세포벽재생에 미치는 BA의 영향을 조사하여, cytokinin이 식물의 세포벽재생을 촉진하는 기능이 있음을 확인하고 세포벽재생에 있어서 cytokinin의 작용기구를 검토하였다. 대두 ${\beta}-1,3-glucanase$는 21 kD의 polypeptide로 동정되었는데 이 polypeptide의 세포내함량과 효소활성은 BA처리에 의하여 저하되었다. 그리고 callus세포벽의 callose함량과 protoplast의 세포벽재생율이 BA처리에 의하여 증가되었다. 이 결과들은 cytokinin이 세포의 ${\beta}-1,3-glucanase$수준을 저하시켜 callose분해를 억제함으로써 세포벽 재생을 촉진할 수 있음을 보여주었다.

• 한국식품저장유통학회지
• /
• 제2권1호
• /
• pp.185-193
• /
• 1995

This paper was investigated the changes of the cell wall components, enzyme activities during ripening of jujuba fruits for elucidating the softening metabolism of jujuba fruits. Firmness were decreased during ripening. Moisture content did not show any notable cahanges until ripening stage but they decreased a little In overripe jujuba fruits. Polygalacturonase activities were not detected at nature green stage and $\beta$-galactosidase activities were until turning stage. But polygalacturonase activities in ripening and overripening were 51.31 and 100.72 units/100g-fr, wt. respectively. $\beta$-galactosidase activities were 16.05 and 182.55units/100g-fr. wt. in the same stages. The content of water-soluble protein was increased in overripening. Stage the contents of cell wall and alcohol-insoluble material were. decraesed during maturation, but water-soluble material was increased. The pectin and alkali-soluble hemicellulose were increased until ripening stage, but decreased in overripe jujube fruits. The total pectin and insoluble pectin during ripening, but decreased in overripe jujuba fruits.

• Journal of Ginseng Research
• /
• 제7권2호
• /
• pp.169-171
• /
• 1983

The structural components of cell wall in various parts of Korean ginseng were analysed. Pectin was abundant in the cortex and epidermis of root, and leaf. Hemicellulose in root was more than in other parts of ginseng. Cellulose content of stem and seed coat was much higher compared to other parts and lignin content was highest in seed coat.

• 한국식품영양과학회지
• /
• 제27권1호
• /
• pp.29-34
• /
• 1998

This study was investigated to know changes of the cell wall components, cell wall degrading enzyme activities and contents of soluble protein of strawberry during ripening and softening. The contents of water soluble substances were slightly increased during ripening, but the contents of alcohol-insoluble substances were not changed. The contents of pectin were not changed at green mature and turning stage, while decreased after mature stage. The contents of alkali-soluble hemicellulose and cellulose were increased during ripening and softening. The contents of water-soluble and saltsoluble protein were not changed, but the content of cell wall protein was slightly decreased during ripening. The content of total protein was increased at turning stage, it is not changed after turning stage. $\beta$-Galactosidase activity was increased during ripening, and pectinmethylesterase activity was decreased at turning. Phenylalanine ammonia-lyase activity was changed up to mature stage, but decreased at overripening stage. Polygalacturonase and cellulase activities were not detected at all of ripening stages.

• 한국식생활문화학회지
• /
• 제6권3호
• /
• pp.229-235
• /
• 1991

건시의 품질에 중요한 영향을 미치는 물성적 특성과 세포벽 구성성분과의 관계에 대하여 연구하였다. 건시의 수분함량은 30-36% 정도였으며 물성특성 중 경도는 수분함량이 낮은 둥시(상주), Hagakure 및 고종시에서 높게 나타났다. 물성특성치 중 부서짐성, 경도, 껌성, 씹힘성은 품종간 유의차가 인정되었으나 응집성, 부착성 및 탄성은 품종에 따른 통계적인 유의차가 나타나지 않았다. 조세포벽의 함량, 세포벽의 펙틴물질 및 칼슘 함량은 경도가 높은 품종에서 높게 나타났으며 펙틴물질 중 수용성 펙틴은 경도가 낮은 품종에서 그리고 산 및 알칼리가용성 펙틴은 경도가 높은 품종에서 높게 나타났다. 주사전자현미경으로 건시과육의 세포벽을 관찰한 결과 경도가 높은 품종의 세포벽이 비교적 견고하게 관찰되었다.

• 한국식품저장유통학회지
• /
• 제4권3호
• /
• pp.301-309
• /
• 1997

This study was conducted to examine morphological characteristics and the content of cell wall polysaccharides of Brassica campestris var. pekinensis(baechu). First of all, the variety of scientific name and naming of parts of baechu in the literatures of kimchi showed, which will unify marks. So, we propose not so much mid-rib and leaf blade of baechu leaf as white part and green part, respectively. On the other hand, the forms of vessel elements of white part in baechu consist in ring, sclariform and reticulate thickening. The proximate compositions and contents of cell wall polysaccharides of baechu has significant differences between its cultivars. The cell wall pectin from baechu exhibited four peals with molecular weights of 2,000,000, about 100,000 and less than 10,000 by gel filteration chromatography and hemicellulose did two peaks with molecular weights of 2,000,000 and 10,000.

• 한국식품저장유통학회지
• /
• 제2권1호
• /
• pp.173-183
• /
• 1995

This paper was carried out to investigate changes in chromatograms of polysacctatides and soluble pectins on Sephadex G-50 and non-cellulosic neutral sugars of polysaccharides isolated from cell wall of persimmon fruits treated with polygalacturonase and $\beta$-galactosidase in vitro. The chromatogram pattern of soluble pectins extracted from cell wall treated with $\beta$-galactosidase on Sephacryl S-500 column were similar to those of untreatment, but contents of soluble pectins treated with $\beta$-galactosidase were different from those of untreatment. The patterns of chromatograms In soluble pectins extracted from cell wall treated with polygalacturonase were more complex and lower molecular polymer than those of other cell wall-degrading enzyme treatments. Non-cellulosic neutral sugar of polysaccharides in fraction I of soluble material treated with polygalacturonase was rhamnose, those in fraction II were similar to those in fraction III and contents of arabinose, xylose and glucose were higher than contents of other non-cellulosic neutral sugars. Non-cellulosic neutral sugars of polysaccharides in fraction I in soluble material by $\beta$-galactosidase treatment were rhamnose, arabinose, galactose and mannose. Content of glucose of polysaccharides in fraction II was higher than that in fraction I . Non-cellulosic neutral sugars treated with mixed enzyme were rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose. Compositions of non-cellulosic neutral sugars of polysaccharides in fraction I were similar to those in fraction II and III.

• Preventive Nutrition and Food Science
• /
• 제9권1호
• /
• pp.92-94
• /
• 2004

Changes in reducing sugar and cell wall degrading enzymes during ripening of banana for 10 days were investigated. The amount of reducing sugar in bananas increased during storage at room temperature during the first 7 days, and decreased thereafter. However, starch content in banana decreased during ripening, and invertase and cell wall degrading enzymes such as cellulase, polygalacturonase and xylanase were most active after bananas were stored for 7 days at room temperature. When the bananas were stored at 4$^{\circ}C$, the magnitude of changes were much less than during room temperature storage.