• Korean Journal of Materials Research
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• v.29 no.11
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• pp.697-702
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• 2019

In this study, using a wet chemical process, we evaluate the effectiveness of different solution concentrations in removing layers from a solar cell, which is necessary for recovery of high-purity silicon. A 4-step wet etching process is applied to a 6-inch back surface field(BSF) solar cell. The metal electrode is removed in the first and second steps of the process, and the anti-reflection coating(ARC) is removed in the third step. In the fourth step, high purity silicon is recovered by simultaneously removing the emitter and the BSF layer from the solar cell. It is confirmed by inductively coupled plasma mass spectroscopy(ICP-MS) and secondary ion mass spectroscopy(SIMS) analyses that the effectiveness of layer removal increases with increasing chemical concentrations. The purity of silicon recovered through the process, using the optimal concentration for each process, is analyzed using inductively coupled plasma atomic emission spectroscopy(ICP-AES). In addition, the silicon wafer is recovered through optimum etching conditions for silicon recovery, and the solar cell is remanufactured using this recovered silicon wafer. The efficiency of the remanufactured solar cell is very similar to that of a commercial wafer-based solar cell, and sufficient for use in the PV industry.

• The Journal of Internal Korean Medicine
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• v.1 no.1
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• pp.98-106
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• 1976

In order to study the effect of croton oil on the mucous cell in the mice intestin the experimental animals were injected with 0.1gm body weight of croton oil through intraperitoneally. They were sacrificed by ether anesthesia and obtained from distal small intestine and duodenum and colon, and fixed in 10% meutral formal. After embedded in paraffin, sectioned in 5 micro thickness, and stained with P.A.S (Periodic Acid Schiffis) reaction. The average number of the mucous cell was counted in each specimen over 20 fields under 450 magnification. The following results were obtained; 1) An average number of mucous cell began rapidly increase from 15 min and reached high average number after injection of croton oil of mucous cell from 30-60 min after injection. 2) An average number of mucous cell rapidly increase after injection of croton oil and were not reduced normal level by time lapsed 48 hrs. 3) The mucous cell showed with tape of time after injection of croton oil. A type and B type decrease and showed recovery C type decrease and recovery. 4) According to the above findings, it is presumed that croton oil accelerate secretion of mucin of the mucouse cell and production of mucin in growing mucous cell.

• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.186-195
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• 2021

In this study, when stem cell culture solution is used as a cosmetic ingredient, one of the most prominent problems is that the ingredients generally have low thermal stability. Therefore, in this study, in order to find out how the stem cell culture medium is heated or preserved at high temperature, the effect of various effects of stem cells on the various effects of the stem cells was investigated. Investigated. As a result of the experiment, the wound healing assay confirmed that the cell migration increased after 6 hours, and after 24 hours, it was confirmed that the cell mobility was increased and cell division was promoted, thereby being concentrated. As a result of investigating the amount of transdermal water loss by preparing a cosmetic product containing stem cell culture solution, it was confirmed that the culture solution addition group showed an improvement rate of 31% compared to the non-added group, thereby helping in skin wound recovery. As a result of this, it is considered that this point should be considered when the stem cell culture medium is used as an active ingredient in cosmetics in the future.

• The Journal of Korean Medicine
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• v.33 no.3
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• pp.133-146
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• 2012

Background: Most antitumor agents have the side effect of chemotherapy-induced peripheral neuropathy (CIPN). Cancer patients who take antitumor agents suffer from CIPN, but there is no known treatment for it. Unlike the central nerve system, the peripheral nerve can self-repair, and the Schwann cell takes this mechanism. Objectives: In this study, we researched the effect of YideungJetong-Tang (YJT) extract on taxol-induced sciatic nerve damage, through in vitro and in vivo experiments. Also, we studied the effect of YJT extract on neurite recovery and anti-inflammatory effect after compression injury of sciatic nerve in vivo. Methods: Vehicle, taxol and taxol+YJT were respectively applied on sciatic nerve cells of rat in vitro, then the cells were cultured. The sciatic nerve cells and Schwann cells were then observed using Neurofilament 200, Hoechst, ${\beta}$ -tubulin, S-$100{\beta}$, caspase-3 and phospho-Erk1/2. CIPN was induced by taxol into the sciatic nerve of rat in vivo, then YJT extract was taken orally. The axons, Schwann cells and neurites of the DRG sensory nerve were then observed using Neurofilament 200, ${\beta}$-tubulin, Hoechst, S-$100{\beta}$, phospho-Erk1/2 and caspase-3. YJT was taken orally after sciatic nerve compression injury, and the changes in axon of the sciatic nerve, Schwann cells and TNF-${\alpha}$ concentration were observed. Results: The taxol and YJT treated group showed significant effects on Schwann cell recovery, neurite growth and recovery. In vivo, YJT compared with control group showed Schwann cell structural improvement and axons recovering effect after taxol-induced Schwann cell damage. After sciatic nerve compression injury, recovery of distal axon, changes of Schwann cell distribution, and anti-inflammatory response were observed in the YJT. Conclusions: Through this study, we found that after taxol-induced neurite damage of sciatic nerve in vivo and in vitro, YJT had significant effects on sciatic nerve growth and Schwann cell structural improvement. In vivo, YJT improved recovery of distal axons and Schwann cells and had an anti-inflammatory effect.

• The Journal of Korean Medicine
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• v.24 no.1
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• pp.190-201
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• 2003

Object : This study was designed to investigate the effects of Chungganhaeju-tang (Qingganjiejiu-tang) on cytotoxicity, growth inhibition, apoptosis and expression of cytokine in damaged HepG2 cells. Method : Toxicity on HepG2 cell induced by ethanol and acetaldehyde was measured for viability, cell growth, DNA replication and generation of apoptosis and cytokine. The recovery of the cell activity by Chungganhaeju-tang was estimated for the measured parameters using PCR with different cycle numbers, DNA gel-electrophoresis, and densitometric analysis, Results : Chungganhaeju-tang improves the recovery of HepG2 cells damaged by ethanol or acetaldehyde. The suppressed DNA synthesis of the cell damaged by ethanol or acetaldehyde is improved by Chungganhaeju-tang. A liver-protection effect was shown by the reduction of apoptosis and $TNF-{\alpha},{\;}IL-1{\beta}$ expressions that are induced by ethanol or acetaldehyde. Conclusion : The result indicates that Chungganhaeju-tang reduces toxicity induced by ethanol or acetaldehyde and recovers damaged liver function.

• Microbiology and Biotechnology Letters
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• v.7 no.3
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• pp.165-173
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• 1979

Recovery component for cell division in UV-irradiated E. coli B was detected with use of the cell extract of E. coli B/r which is a resistant mutant of E. coli B against UV-irradiation. The active substance was non-dialyzable and increased the activity by adding B-NAD remarkably. One more factor for increasing or promoting the restoration recognized was magnesium. Magnesium was effective to stabilze the substance in procedure of isolation. Two active substances were obtained from sucrose gradient centrifugation. One of them was recovred from the botton area and the other from top area just below below surface. the former was not stabilized by magnesium, while the latter stabilized the activity by it remarkably. The former which did not require magnesium was insensitive to protease and the latter which required magnesium was sensitive to it. Both were insensitive to RNase and DNase. Recovery ratio was doubled by using nitrogen gas than aeration in purification process. DNA-ligase less mutant was revealed same activity on it's recovery ratio with the parent strain of E. coli K-12. The active substance stimulating the filament cell may exist as a complex which is inactivated easily in the dissociated state ana requrie B-NAD or magnesium.

• KSBB Journal
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• v.15 no.4
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• pp.366-369
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• 2000

The soluble paclitaxel was found in the supernatant of the plant cell cultures of Taxus chinensis, The percentage of soluble paclitaxel depends on paclitaxel concentration in bioreactor. As paclitaxel concentration decreases the percentage of soulbe paclitaxel increases. it is therefore important to develop a new process for the recovery of soluble paclitaxel. The use of hydrophobic resin HP20 gives nearly perfect recovery of paclitaxel in supernatant. The resin was more effective in treatment of th cell and debris free filtrate probably because of the reduced solids content In this case 3 g.l resin and 1 day reaction were enough for recovery the soluble paclitaxel in medium.

• The Journal of Korean Obstetrics and Gynecology
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• v.23 no.1
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• pp.30-41
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• 2010

Purpose: This study was to observe the effect of "herbal decoction for sitz bath" on dermoepidermal recovery to wound tissue in rat's skin. Methods: The samples were assigned to 3 groups: control group : without any treatment, positive control group : potarose 10% solution, experiment group : herbal decoction for sitz bath. We made the open wound of $2{\times}2cm^2$ size that cut deep into the dermis. Treating the open wound for 17 days, we observed the size of the wound diminishing. On 17th days, the cell viability was measured by MTT assay. The effect anti-inflammatory and dermoepidermal recovery were examined by H&E staining, immunohystochemical staining for MIP-2, FGF. Results: The experiment group showed more recovery from the open wound comparing the control group and the positive control group on 10th days after wounding. But there was not remarkable difference between the experiment and positive control group after 17th days post-wounding. The number of MIP-2 positive reacted cell were significantly decreased and that of FGF positive reacted cell were significantly increased than positive control group at 17th days. Conclusion: According to these results, we finally concluded that "herbal decoction for sitz bath" could be effective in recovery to wound tissue.

• Korean Journal of Veterinary Research
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• v.33 no.1
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• pp.171-178
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• 1993

The present study was carried out to investigate the developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos. The nuclei from 2-, 4- and 8-cell mouse embryos were transferred into enucleated 2-cell embryos, and the reconstituted embryos were submitted to direct current(DC) pulse at output voltage of 2.0 kV/cm for $100{\mu}$ sec to induce cell fusion. The recovery rate and developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos was investigated. 1. The recovery rate of nuclear transplanted 2-cell embryos in normal morphology after freezing and thawing was significantly higher in rapid freezing(DMSO 4.5M) than in slow cooling(p<0.01). 2. When the recovered embryos in normal morphology were cultured in vitro, there were no significant differences in the developmental potency to blastocyst between the freezing methods and the concentrations of cryoprotectant. In summary, these experiments have proved that rapid freezing method(DMSO 4.5M) is effective in nuclear transplanted 2-cell mouse embryos. If improved micromanipulation techniques and freezing are combined, nuclear transplantation technique will contribute to the improvement of productivity in livestock animals.

• Journal of the Korean Applied Science and Technology
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• v.29 no.1
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• pp.102-107
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• 2012

The recovery of a Pt anode in a PEMFC through 30 ppm $H_2S/H_2$ exposure was evaluated by using a cyclic voltametry(CV) scan. First, the PEMFC unit cell performanc loss was measured three times under an anode feeding with 30 ppm $H_2S/H_2$ for 1hr at $0.5A/cm^2$ of current density. The initial cell performance was $1.16A/cm^2$ at 0.6 V without $H_2S$ poisoning. After first poisoning step for 1hr the cell performance was decrease to $0.77A/cm^2$, and the further poisoning steps decreased up 0.57 V. Finally, the recovery of the cell performance of $H_2S$ poisoned PEMFC was achieved up to 90.3% by applying CV scan. Moreover, we also found out that another possible approach for over 80% recovery of the cell performance of $H_2S$ poisoned anode Pt catalyst layer was to just inject fresh hydrogen into the anode feeding stream.