• 제목/요약/키워드: cell recovery

검색결과 906건 처리시간 0.029초

Optimization of an Intact Cell System of Rhodocyclus gelatinosus KUP-74 for ${\delta}-Aminolevulinic$ Acid Production

  • Lim, Wang-Jin;Choi, Kyung-Min;Hwang, Se-Young
    • Journal of Microbiology and Biotechnology
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    • 제3권4호
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    • pp.244-251
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    • 1993
  • A novel system has been developed to produce ${\delta}-aminolevulinic$ acid (ALA) using the intact cells of late logarithmic phase of Rhodocyclus gelatinosus KUP-74. The system was shown optimum yield of extracellular ALA under a condition of anaerobic light irradiation (4 Klux) at $30^{\circ}C$ with no variation in cell mass. The rate of extracellular ALA formation was stimulated by low doses of either $C_4\;or\;C_5$ ALA biosynthetic precursors, where 5 mM ($C_4) and 3 mM ($C_5) of each precursors were appeared to generate the maximum rates of 3.3 and 4.0 nmoles of ALA per 0.35 mg cells per hr, respectively. Half-life of the system was 10 hr in a sense of an ability of portage transport of L-glutamate, and sequential dose of this compound was resulted in promising recovery of the ALA.

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과배란 처리된 체내 초기배 발생에 관한 연구 (Development of Early Embryos inIn Vivo Superovulated Rabbits)

  • 조현조;이홍준;심금섭
    • 한국수정란이식학회지
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    • 제9권2호
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    • pp.167-172
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    • 1994
  • This experiment was arried out to investigate the development of ea4y rabbit embryos in vivo. Twenty-six New Zealand White does were superovulated by treatment with PMSG(Intervet Co; I. M single injection, 150. U./rabbit) followed 3 day later by simultaneous I.V injection of 100 I.U HCG (Intervet Co, )and natural service with fertile male. All of does was killed at the specific times (24, 27, 30, 36, 42, 50 and 93 h post-hCG) to find out the early embryonic development in vivo respectively. Embryos at the specified stages of development were obtained at the following times after injection of hCG; one-ceH at 24 h, two-cell at 24~27h, four-cell at 27~36 h, morulae at 50 h and early blasto-cyst at 93 h and expanded or hatching blastocyst at 144 h. Number of embryos recovered per rabbit superovulated was 26.1 and average of recovery rate was 83.7%. The results suggest that superovulation was efficient for the increase of embryo number in rabbits, and as shown in results, asynchronous cleavage was prevalent among the recovered embryos.

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액체수소의 Exergy를 이용한 연료전지 시스템의 성능향상 (The Performance Improvement of Fuel Cell System by using LH2 Exergy)

  • 박동필;정귀성;오병수
    • 한국수소및신에너지학회논문집
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    • 제12권3호
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    • pp.211-217
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    • 2001
  • From wood to coal and petroleum, mankind has used various fuel. Since using such fuel, mankind has developed power source of mechanism. We obtain numerous power from caloric force. Present energy supply is based on the fossil fuel. Fossil fuel has high energy density and is convenient for transportation and storage. Human being prepared countermeasure of energy economy, high energy efficiency and substitution energy for limits of fossil fuel. High energy efficiency among them is very important. This research will improve total output by physical exergy recovery of $LH_2$-fuel cell system.

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PRESENT AND FUTURE OF SUPER HIGH-EFFICIENCY TANDEM SOLAR CELLS

  • Yamaguchi, Masafumi
    • E2M - 전기 전자와 첨단 소재
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    • 제11권11호
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    • pp.37-45
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    • 1998
  • In this paper, present status of super high-efficiency tandem solar cells has been reviewed and key issues for realizing super high-efficiency have also been discussed. In addition, the terretrial R&D activities of tandem cells, in the New Sunshine Program of MITI(Ministry of International Trade and Industry) and NEDO(New Energy and Industrial Technology Development Organization) in Japan are reviewed briefly. The mechanical stacked 3-junction cells of monolithically grown InGaP/GaAs 2-junction cells and InGaAs cells have reached the highest efficiency achieved in Japan of 33.3% at 1-sun AM1.5. This paper also reports high-efficiency InGaP/GaAs 2-junction solar cells with a world-record efficiency of 26.9% at AM0, 28$^{\circ}C$ and radiation damage recovery phenomena of the tandem cell performance due to minority-carrier injection under light illumination or forward bias, which causes defect annealing in InGaP top cells. Future prospects for realizing super-high efficiency and low-cost tandem solar cells are also described.

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Diethylhexyl Phthalate 처치후 건양단이 정자 생성능 및 운동성에 미치는 영향 연구 (Protective Effect of KunYangDan on Motility and Viability of Sperms Exposed by Diethylhexyl Phthalate)

  • 남창욱;박경수;마진열
    • 한국한의학연구원논문집
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    • 제12권1호
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    • pp.91-102
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    • 2006
  • We treated KunYangDan (KYK) in SD rats in order toexamine the protective effect against cell damage induced by diethylhexyl phthalate (DEHP). KYD reduced DEHP toxicity by increasing the function of immune cell numbers weight increase of spleen.red blood cells,HB,and HCT content,sperm number and mobility,resulting in improving reproductive function by judging from the recovery of testosterone content. Interestingly the hormone change of testosterone by KYD significantly recovered the decreased its leve. Taken together,these results suggest that KYD specifically affect the reproductive function induced by DEHP,an endocrine disruptor.

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식물-미생물전기화학 기반의 미활용 에너지 회수 기초 연구 (Basic Study for Harvesting Unused Energy based on Plant-Microbial Electrochemical Technology)

  • 유재철;신춘환
    • 한국환경과학회지
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    • 제28권2호
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    • pp.219-224
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    • 2019
  • In this study, we evaluated the energy production from plant-microbial fuel cells using representative indoor plants, such as Scindapsus aureus and Clatha minor. The maximum power density of microbial fuel cell (MFC) using S. aureus ($3.36mW/m^2$) was about 2 times higher than that of the MFC using C. minor ($1.43mW/m^2$). It was confirmed that energy recovery is possible using plant-MFCs without fuel. However, further research is needed to improve the performance of plant-MFCs. Nevertheless, plant-MFCs have proved their potential as a novel energy source to overcome the limitations of the conventional renewable energy sources such as wind power and solar cells, and could be employed to a power source for the sensor in charge of the fourth industrial revolution.

Evaluation of the Antidiabetic, Islet Protective and Beta-Cell Regenerative Effects of Tinospora crispa (L.)

  • Yusof, Norafiqah;Goh, May P. Y.;Ahmad, Norhayati
    • Natural Product Sciences
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    • 제28권3호
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    • pp.105-114
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    • 2022
  • Tinospora crispa (L.) is a medicinal plant traditionally used to treat various ailments including diabetes. The stem has been widely studied for its antidiabetic properties, however the antidiabetic potential of its leaves has not been explored. This study investigates the antidiabetic properties of methanolic T. crispa stem and leaves extracts on alloxan-induced diabetic rats. The stems and leaves were extracted using Soxhlet extraction with methanol as solvent. Crude extracts were administered at 500 mg/kg body weight (BW) (high dose) and 250 mg/kg BW (low dose) via oral route to alloxan induced diabetic rats. T. crispa stem and leaves extracts was found to significantly reduce blood glucose following a twelve-week treatment period. The highest mean difference in blood glucose level was exhibited by animals in the high dose treated stem and low dose leaf extracts. Both extracts showed approximately 75% percentage recovery from hyperglycaemia. The highest regenerative capacity was observed in animals treated with the low dose leaf extract.

Reverse Facial-submental Artery Island Flap with Reinnervation of the Anterior Belly of the Digastric Muscle

  • Sakuma, Hisashi;Takemaru, Masashi
    • Archives of Plastic Surgery
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    • 제49권3호
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    • pp.423-426
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    • 2022
  • Reconstruction of the upper lateral lip subunit is challenging, and use of several classical local flaps have been previously reported. However, these methods have drawbacks such as visible scarring, anatomic distortion, and functional disability. To obtain satisfactory results, preservation of perioral function is important. We report a case of functional upper lip reconstruction after tumor resection using a reverse facial-submental artery island flap with a reinnervated anterior belly of the digastric muscle (ABDM) without sacrificing the perioral structure. A 73-year-old man presented with basal cell carcinoma on the left upper lip which was widely excised, including the orbicularis oris muscle. The remaining 4 cm × 3.5 cm defect was reconstructed using a reverse facial-submental artery island flap with ipsilateral ABDM. The motor nerve of the ABDM was sutured with the stump of the buccal branch of the ipsilateral facial nerve. The postoperative course was uneventful, and good functional and esthetic recovery were observed at 12-month follow-up. This procedure may be an alternative option for reconstruction of lateral upper lip defects.

Primary astrocytic mitochondrial transplantation ameliorates ischemic stroke

  • Eun-Hye Lee;Minkyung Kim;Seung Hwan Ko;Chun-Hyung Kim;Minhyung Lee;Chang-Hwan Park
    • BMB Reports
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    • 제56권2호
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    • pp.90-95
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    • 2023
  • Mitochondria are important organelles that regulate adenosine triphosphate production, intracellular calcium buffering, cell survival, and apoptosis. They play therapeutic roles in injured cells via transcellular transfer through extracellular vesicles, gap junctions, and tunneling nanotubes. Astrocytes can secrete numerous factors known to promote neuronal survival, synaptic formation, and plasticity. Recent studies have demonstrated that astrocytes can transfer mitochondria to damaged neurons to enhance their viability and recovery. In this study, we observed that treatment with mitochondria isolated from rat primary astrocytes enhanced cell viability and ameliorated hydrogen peroxide-damaged neurons. Interestingly, isolated astrocytic mitochondria increased the number of cells under damaged neuronal conditions, but not under normal conditions, although the mitochondrial transfer efficiency did not differ between the two conditions. This effect was also observed after transplanting astrocytic mitochondria in a rat middle cerebral artery occlusion model. These findings suggest that mitochondria transfer therapy can be used to treat acute ischemic stroke and other diseases.

낸드 플래시 파일 시스템을 위한 결함 복구 시간 제한 기법의 설계 및 구현 (Design and Implementation of Crash Recovery Technique with Bounded Execution Time for NAND Flash File System)

  • 강승엽;박현찬;김기만;유혁
    • 한국정보과학회논문지:시스템및이론
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    • 제37권6호
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    • pp.330-338
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    • 2010
  • 현재 낸드 플래시 메모리는 다양한 이동형 기기에 활용되면서 급격하고 지속적인 성장을 하고 있다. 사용자들은 더욱 용량이 크고 빠른 기기들을 요구하지만, 현재의 낸드 플래시 파일 시스템은 결함 상황에서 복구 및 초기화 시간이 미디어의 용량에 비례하여 지연되는 문제가 있으며 이는 기기의 부팅 시간을 지연시켜 사람들의 불편을 초래한다. 우리는 이를 해결하기 위해 파일 시스템 초기화 시간이 미디어의 용량과 무관하도록 '작업 영역 기법'을 제안한다. 작업 영역 기법은 한 시점에서 낸드 플래시 메모리의 일부 영역만을 작업 공간으로 정의하고 유지하며 쓰기 명령을 작업 공간 안에서만 수행시킨다. 따라서 결합 상황에서 복구시 검색해야 할 크기를 최근 작업 영역으로 제한하므로써 결함 복구 수행 시간이 낸드 플래시 메모리 용량과 비례하지 않도록 한다. 우리는 이러한 작업 영역 기법을 YAFFS2를 기반으로 구현하였으며 기존 기법들과 초기화 성능을 비교해 보았다. 그 결과 1기가 낸드 플래시 메모리상에서 결함 복구 수행 시간이 기존의 로그 기반 기법에 비해 25배 가량 단축됨을 확인했다. 이러한 격차는 낸드 플래시 메모리 용량이 늘어날수록 커질 것이다.