• Journal of Ginseng Research
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• v.47 no.1
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• pp.89-96
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• 2023

Background and aim: Panax ginseng, a key herbal medicine of replenishing Qi and tonifying Spleen, is widely used in the treatment of gastrointestinal diseases in East Asia. In this study, we aim to investigate the potential effects and mechanisms of polysaccharides from P. ginseng (PGP) on intestinal mucosal restitution which is one of the crucial repair modalities during the recovery of mucosal injury controlled by the Ca²⁺ signaling. Methods: Rat model of intestinal mucosal injury was induced by indomethacin. The fractional cell migration was carried out by immunohistochemistry staining with BrdU. The morphological observations on intestinal mucosal injury were also performed. Intestinal epithelial cell (IEC-6) migration in vitro was conducted by scratch method. Western-blot was adopted to determine the expressions of PLC-𝛾1, Rac1, TRPC1, RhoA and Cav-1. Immunoprecipitation was used to evaluate the levels of Rac1/PLC-𝛾1, RhoA/TRPC1 and Cav-1/TRPC1. Results: The results showed that PGP effectively reduced the assessment of intestinal mucosal injury, reversed the inhibition of epithelial cell migration induced by Indomethacin, and increased the level of Ca²⁺ in intestinal mucosa in vivo. Moreover, PGP dramatically promoted IEC-6 cell migration, the expression of Ca²⁺ regulators (PLC-𝛾1, Rac1, TRPC1, Cav-1 and RhoA) as well as protein complexes (Rac1/PLC-𝛾1, Cav-1/TRPC1 and RhoA/TRPC1) in vitro. Conclusion: PGP increases the Ca²⁺ content in intestinal mucosa partly through controlling the regulators of Ca²⁺ mobilization, subsequently promotes intestinal epithelial cell migration, and then prevents intestinal mucosal injury induced by indomethacin.

• Korean Journal of Animal Reproduction
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• v.24 no.3
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• pp.263-268
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• 2000

The development of single blastomeres isolated from 2-, 4- and 8-cell mouse embryos and the ability of such blastomeres to survive slow freezing were studied. Of 223, 60 and 188 single blastomeres isolated from 2-, 4- and 8-cell mouse embryos, respectively, 111 blastomeres (49.8%) from 2-cell embryos, 12 blastomeres (20.0%) from 4-cell embryos and blastomeres (16.5) from 8-cell embryos developed into blastocysts after culture for 96 hrs. The recovery rate was 54.2% (65/120), 46.4% (13/28) and 24.3% (17/70) of blastomeres derived from 2-, 4- and 8-cell embryos following freezing and thawing and the survival of frozen-thawed blastomeres was 27.1% (16/59), 36.4% (4/11) and 17.6% (3/17), and respectively. The apparently six normal fetuses were obtained from frozen-thawed blastomere from 2-cell embryos after transferring into the recipients. These results indicate that mouse btastomeres isolated from preimplatation stage embryos can survive storage in liquid nitrogen following slow freezing.

• Journal of Evidence-Based Herbal Medicine
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• v.2 no.1
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• pp.43-49
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• 2009

Sodium arsenate and Yeonlyeonggobon-dan (nianlinggubendan) extract (YGD), a herbal restorative were treated p.o. 20 mg/kg and 500 mg/kg, respectively, and concurrently to rats, and examined the biochemical parameters in blood. The values of white blood cell (WBC), red blood cell (RBC), hemoglobin (Hgb) and hematocrit (Hct) in each group did not show significant variance. The value of aspartate aminotrasferase (AST) of arsenic-treated group was increased for 2 weeks significantly while that of the group of concurrent administration with YGD became low significantly compared with arsenic-treated group and the value of alkaline phosphatase (ALP) of arsenic-treated group was decreased while that of the group of concurrent administration with YGD was increased significantly compared with arsenic-treated group. In arsenic-treated groups, the value of glucose (Glu), and those of lactic dehydrogenase (LDH), blood urea nitrogen (BUN) and triglyceride (TG) were decreased at first but increased later while the groups of concurrent administration with YGD showed significant recovery from the toxicity of arsenic.

• Laser Solutions
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• v.1 no.1
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• pp.18-23
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• 1998

A Study on microstructural changes of sensitized Alloy 600 which was rapidly solidified by a $CO_2$ laser beam was conducted using microscopic equipments such as SEM and TEM. Dissolution of Cr-rich carbides and resultant Cr recovery on the grain boundaries occurred in the heat affected zone (HZA). The microstructure of the laser melted zone (LMZ) having epitaxially solidified from the HAZ was mainly celluar-dendritic with the 〈100〉 crystallographic direction of growth. The Cr concentration was observed to increase along the cell bondaries, and tiny particles were distributed along the cell walls with tangled dislocations around them. Cr-rich carbides had been completely melted by the high density of a laser beam, and were not re-precipitated during the matrix solidification due to a fast cooling rate in the LMZ.

• Proceedings of the Korean Society Of Semiconductor Equipment Technology
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• 2007.06a
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• pp.244-249
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• 2007

In this paper, in order to get the characteristics of the lithium secondary cell, such as cycle life, charge and discharge characteristic, temperature characteristic, self-discharge characteristic and the capacity recovery rate etc, we build a mathematical model of battery. In this one-dimensional model, Seven governing equations are made to solve seven variables $c,\;c_s,\;{\Phi}_1,\;{\Phi}_2,\;i_2,\;j\;and\;T$. The mathematical model parameters used in this model have been adjusted according to the experimental data measured in our lab. The connecting research of this study is to get an accurate estimate of the capacity of battery through comparison of results from simulation and fuzzy logic system. So the result data from this study is reorganized to fit the fuzzy logic algorithm.

• Advances in Traditional Medicine
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• v.5 no.1
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• pp.48-56
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• 2005

We studied the protective effect of Echinacea purpurea against radiation by evaluating changes in the peripheral blood cell count and peripheral blood antioxidant activity. Echinacea purpurea administration had a suppressive effect on radiation-induced leukopenia, especially on lymphocytes and monocytes and resulted in a faster recovery of blood cell counts. Mouse peripheral blood antioxidant activity was increased by Echinacea purpurea, and a relationship between the suppressive effect on radiation-induced leukopenia and the antioxidant effect was suggested.

• KSBB Journal
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• v.29 no.3
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• pp.220-224
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• 2014

Flocculation is known one of the effective methods for harvesting microalgae. This study was aimed to optimize the flocculation condition for decreasing the amounts of flocculant and obtaining the highest yield of algal biomass. To achieve this goal, it was optimized the flocculant concentration, reaction pH and the concentration of cell density for harvest using response surface methodology (RSM). The flocculation of microalgae, Spirogyra varians, was carried out using inorganic flocculant polyaluminium chloride. By the RSM result, the optimal flocculation condition was calculated 5 ppm of polyaluminum chloride, pH 7.5 and 0.33 of optical cell density at $OD_{640}$. The obtained recovery yield of S. varians was 97.6% at the optimal condition.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.816-820
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• 2005

Polysaccharide-based dressings have increasingly become viable alternatives to somewhat less biocompatible and often problematic cotton or viscose gauzes traditionally used for wound dressings. Polysaccharide which is particularly abundunt in Agaricus blazei Murill is known as the bioactive materials. Polysaccharide extracted from Agaricus blazei Murill enhances the recovery of the damaged epidermal tissue and the production of epithelial cell growth factors which are necessary to heal the burn and wound on the epidermal tissue. In this study, the biopolymer delivery system of polysaccharide extracted from Agaricus blazei Murill into epidermal tissue was elucidated using the skin permeating enhancer in vitro. The enhancing effects of various penetration enhancers and some other enhancers on the permeation of ${\beta}-glucan$ were evaluated using Franz diffusion cell. Permeation of polysaccharide in nomal and burn skin was compared. Polysaccharide was studied for the burn and wound healing activity in the epidermal tissue on rats.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.3
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• pp.456-464
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• 2008

Avian and mammalian skeletal muscles exhibit a remarkable ability to adjust to physiological stressors induced by growth, exercise, injury and disease. The process of muscle recovery following injury and myonuclear accretion during growth is attributed to a small population of satellite cells located beneath the basal lamina of the myofiber. Several metabolic factors contribute to the activation of satellite cells in response to stress mediated by illness, injury or aging. This review will describe the regenerative properties of satellite cells, the processes of satellite cell activation and highlight the potential role of satellite cells in skeletal muscle growth, tissue engineering and meat production.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2871-2877
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• 2013

Objectives: To evaluate the safety, efficacy, and invasiveness of lobectomy by video-assisted thoracoscopic surgery (VATS) in the treatment of stage I/II non-small cell lung cancer (NSCLC). Methods: A total of 148 patients presenting with Stage I or II NSCLC were enrolled into our study, comprising 71 who underwent VATS and 77 patients undergoing conventional thoracotomic lobectomy, in combination with systematic lymph node resection. Results: It was found that VATS was superior to conventional thoracotomy in terms of the duration of surgery, intraoperative blood loss, frequency of the need to administer postoperative analgesia, thoracic intubation indwelling time, post-operative hospital stay, and survival rate (P<0.05). We saw no obvious difference in the number of resected lymph nodes with either approach. Conclusions: VATS lobectomy is a safe and reliable surgical approach for the treatment of Stage I/II NSCLC, characterized by significantly minimal invasiveness, rapid post-operative recovery, and markedly lower loss of blood.