• The Journal of Korean Obstetrics and Gynecology
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• v.33 no.3
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• pp.60-79
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• 2020

Objectives: This review plans to assess the efficacy and effectiveness of oriental medicine for the treatment of Ovarian Hyperstimulation Syndrome (OHSS) through literature research and overview. Methods: Database searching was conducted to identify relevant randomized controlled trials (RCTs) on oriental medicine for the treatment of Ovarian Hyperstimulation Syndrome. Studies were searched from Journal of Korean Obstetrics and Gynecology, Korean Medical Database, Korean studies Information Service System, China National Knowledge Infrastructure, Cochrane library, PubMed and EmBase up to 21^st May, 2020. Results: Seventeen studies were finally selected. Fifteen studies intervened with oral Chinese herb medicine, two studies intervened with acupuncture and moxibustion. Nine studies concluded that intervention with oriental medicine significantly relieved OHSS symptoms. Three studies reporting ovary diameter, four studies reporting abdominal circumference and other four studies reporting pelvic effusion showed significant reduction compared to control groups. Six studies showed significantly shorter duration for hospitalization in intervention groups. Only one study showed significantly higher pregnancy rate. Factors related with vascular permeability and blood cell coagulation were significantly lowered in intervention groups in general. Conclusions: From seventeen studies, oriental medicine relieved OHSS symptoms and showed treatment effectiveness. Further strictly designed studies and long-term observed studies are needed to establish evidences.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.45 no.3
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• pp.142-148
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• 2008

Though a deblocking filter of H.264/AVC provides enhanced image quality by removing blocking artifact on block boundary, the complex filtering operation on this process is a dominant factor of the whole decoding time. In this paper, we designed an ASIP to accelerate deblocking filter operation with the proposed instruction set. We designed a processor based on a MIPS structure with LISA, simulated a deblocking later model, and compared the execution time on the proposed instruction set. In addition, we generated HDL model of the processor through CoWare's Processor Designer and synthesized with TSMC 0.25um CMOS cell library by Synopsys Design Compiler. As the result of the synthesis, the area and delay time increased 7.5% and 3.2%, respectively. However, due to the proposed instruction set, total execution performance is improved by 18.18% on average.

• Development and Reproduction
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• v.21 no.3
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• pp.237-247
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• 2017

Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicular expression pattern of the genes. From the subtracted cDNA library of rat testis prepared using representational difference analysis (RDA) method, a complimentary DNA clone encoding type III member of a DnaJ family protein, DnaJC18, was cloned (GenBank Accession No. DQ158861). The full-length DnaJC18 cDNA has the longest open reading frame of 357 amino acids. Tissue and developmental Northern blot analysis revealed that the DnaJC18 gene was expressed specifically in testis and began to express from postnatal week 4 testis, respectively. In situ hybridization studies showed that DnaJC18 mRNA was expressed only during the maturation stages of late pachytene, round and elongated spermatids of adult rat testis. Western blot analysis with DnaJC18 antibody revealed that 41.2 kDa DnaJC18 protein was detected only in adult testis. Immunohistochemistry study further confirmed that DnaJC18 protein, was expressed in developing germ cells and the result was in concert with the in situ hybridization result. Confocal microscopy with GFP tagged DnaJC18 protein revealed that it was localized in the cytoplasm of cells. Taken together, these results suggested that testis specific DnaJC18, a member of the type III DnaJ protein family, might play a role during germ cell maturation in adult rat testis.

• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.153-159
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• 2001

In order to clone the peptide synthetase gene form Lysobacter lactamgenus IFO 14,288, the gene fragments were amplified using primers for the adenylation domain and the thionylation domain of the peptide synthetase genes in other organisms by polymerase chain reaction (PCR). The resulting 0.5-kb fragment was cloned in a pGEM-T vector, and the nucleotide sequences were determined. Six different PCR products were obtained; three were identified to be a part of L-$\alpha$-aminoadipyl-L-cysteinyl-D-valine (ACV) synthetase and three to be other peptide synthetases. Using each of the two different classes of PCR products as mixed probes, a cosmid library of L. lactamgenus chromosomal DNA constructed in a pHC79 vector was screened by an in situ hybridization procedure, and one positive clone was selected which was bound by peptide synthetase gene fragments as well as ACV synthetase gene fragments. The partial sequence analysis formt he obtained pPTS-5 cosmid showed th presence of more than two open reading frames. These were for two putative membrane transporters, which were homologous with several integral membrane proteins including the ABC transporter ATP-binding protein of E. coli (YbjZ) and the metal ion uptake protein of Bacillus subtilis (YvrN). A 45% homology was also found between the two transporter proteins at the carboxy terminus. Through a hydropathy analysis and transmembrane analysis. 4-5 transmembrane domains were found in these two proteins. When the genes were expressed in Escherichia coli, the gene products inhibited the hose cell growth, probably due to the disturbance of the membrane transport system.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2005.09a
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• pp.139-143
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• 2005

Cytochrome P450 14${\alpha}$-sterol demethylase enzyme (CYP51) is the target a of azole type antifungals. The azole blocks the ergosterol synthesis and thereby inhibits fungal growth. A three-dimensional (3D) homology model of CYP51 from Candida albicans was constructed based on the X-ray crystal structure of CYP51 from Mycobacterium tuberculosis. Using this model, the binding modes for the substrate (24-methylene-24, 25-dihydrolanosterol) and the known inhibitors (fluconazole, voriconazole, oxiconazole, miconazole) were predicted from docking. Virtual screening was performed employing Structure Based Focusing (SBF). In this procedure, the pharmacophore models for database search were generated from the protein-ligands interactions each other. The initial structure-based virtual screening selected 15 compounds from a commercial available 3D database of approximately 50,000 molecule library, Being evaluated by a cell-based assay, 5 compounds were further identified as the potent inhibitors of Candida albicans CYP51 (CACYP51) with low minimal inhibitory concentration (MIC) range. BMD-09-01${\sim}$BMD-09-04 MIC range was 0.5 ${\mu}$g/ml and BMD-09-05 was 1 ${\mu}$g/ml. These new inhibitors provide a basis for some non-azole antifungal rational design of new, and more efficacious antifungal agents.

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.259-268
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• 2000

Senescence is a sequence of biochemical and physiological events that lead to death of a cell, organ, or whole organism. Senescence is now clearly regarded as a genetically determined and evolutionarilly acquired developmental process comprising the final stage of development. However, in spite of the biological and practical importance, genetic mechanism of senescence has been very limited. Through forward and reverse genetic approaches, we are trying to reveal the molecular and genetic mechanism of senescence in plants, employing leaf organs of Arabidopsis as a model system. Using forward genetic approach, we have initially isolated several delayed senescence mutants either from T-DNA insertional lines or chemical-mutagenized lines. In the case of ore 4 and ore 9 mutants, the mutated genes were identified. The recent progress on characterization of mutants and identification of the mutated genes will be reported. We are also screening mutations from other various sources of mutant pools, such as activation tagging lines and promoter trap lines. Two dominant senescence-delayed mutants were isolated from the activation tagging pool. Cloning of the genes responsible for this phenotype is in progress. For reverse genetic approach, the genes that induced during leaf senescence were first isolated by differential screening method. We are currently using PCR-based suppression subtractive hybridization, designed to enrich a cDNA library for rare differentially expressed transcripts. Using this method, we have identified over 35 new sequences that are upregulated at leaf senescence stage. We are investigating the function of these novel genes by systemically generating antisense lines.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.3
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• pp.427-433
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• 2002

This paper describes a design of cryptographic processor that implements the AES(Advanced Encryption Standard) block cipher algorithm "Rijndael". To achieve high throughput rate, a sub-pipeline stage is inserted into the round transformation block, resulting that the second half of current round function and the first half of next round function are being simultaneously operated. For area-efficient and low-power implementation, the round block is designed to share the hardware resources in encryption and decryption. An efficient scheme for on-the-fly key scheduling, which supports the three master-key lengths of 128-b/192-b/256-b, is devised to generate round keys in the first sub-pipeline stage of each round processing. The cryptoprocessor designed in Verilog-HDL was verified using Xilinx FPGA board and test system. The core synthesized using 0.35-${\mu}{\textrm}{m}$ CMOS cell library consists of about 25,000 gates. Simulation results show that it has a throughput of about 520-Mbits/sec with 220-MHz clock frequency at 2.5-V supply.-V supply.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.4
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• pp.765-770
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• 2012

In this paper, we proposed an effective hardware structure using DCT-based inra-prediction mode selection to reduce computational complexity caused by intra mode decision. In this hardware structure, the input block is transformed at first and then analyzed to determine its texture directional tendency. the complexity has solved by performing intra prediction in only predicted edge direction. $4{\times}4$ DCT is calculated in one cycle using Multitransform_PE and Inta_pred_PE calculates one prediction mode in two cycles. Experimental results show that the proposed Intra prediction encoding needs only 517 cycles for one macroblock encoding. This architecture improves the performance by about 17% than previous designs. For hardware implementation, the proposed intra prediction encoder is implemented using Verilog HDL and synthesized with Megnachip $0.18{\mu}m$ standard cell library. The synthesis results show that the proposed architecture can run at 125MHz.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.9
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• pp.1993-1999
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• 2011

This paper describes a design of area-efficient/low-power cryptographic processor for HIGHT block cipher algorithm, which was approved as standard of cryptographic algorithm by KATS(Korean Agency for Technology and Standards) and ISO/IEC. The HIGHT algorithm, which is suitable for ubiquitous computing devices such as a sensor in USN or a RFID tag, encrypts a 64-bit data block with a 128-bit cipher key to make a 64-bit cipher text, and vice versa. For area-efficient and low-power implementation, we optimize round transform block and key scheduler to share hardware resources for encryption and decryption. The HIGHT64 core synthesized using a 0.35-${\mu}m$ CMOS cell library consists of 3,226 gates, and the estimated throughput is 150-Mbps with 80-MHz@2.5-V clock.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.6
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• pp.1355-1362
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• 2011

This paper describes a multi-mode LDPC decoder which supports three block lengths(648, 1296, 1944) and four code rates(1/2, 2/3, 3/4, 5/6) of IEEE 802.11n WLAN standard. Our LDPC decoder adopts a block-serial architecture based on min-sum algorithm and layered decoding scheme. A novel way to store check-node values and parity check matrix reduces the sizes of check-node memory and H-ROM. An efficient scheme for check-node memory addressing is used to achieve stall-free read/write operations. The designed LDPC decoder is verified by FPGA implementation, and synthesized with a $0.18-{\mu}m$ CMOS cell library. It has 219,100 gates and 45,036 bits RAM, and the estimated throughput is about 164~212 Mbps at 50 MHz@2.5v.