• 한국정보통신학회논문지
• /
• 제17권2호
• /
• pp.355-362
• /
• 2013

TOF(Time-Of-Flight) 센서에 의해 획득된 정보로부터 3차원 깊이 영상(depth image)을 추출하기 위한 위상 연산기의 하드웨어 구현을 기술한다. 설계된 위상 연산기는 CORDIC(COordinate Rotation DIgital Computer) 알고리듬의 vectoring mode를 이용하여 arctangent 연산을 수행하며, 처리량을 증가시키기 위해 pipelined 구조를 적용하였다. 고정 소수점 MATLAB 모델링과 시뮬레이션을 통해 최적 비트 수와 반복 횟수를 결정하였다. 설계된 위상 연산기는 MATLAB/Simulink와 FPGA 연동을 통해 하드웨어 동작을 검증하였으며, TSMC 0.18-${\mu}m$ CMOS 셀 라이브러리로 합성하여 약 16,000 게이트로 구현되었고, 200MHz@1.8V로 동작하여 9.6 Gbps의 연산 성능을 갖는 것으로 평가되었다.

• Journal of Photoscience
• /
• 제7권2호
• /
• pp.53-58
• /
• 2000

We performed the cloning of a chalcone synthase (CHS) gene, the key enzyme in the anthocyanin biosynthesis, from the cDNA library constructed with grape suspension cells irradiated UV-B. The PCR fragment was used to cloning the CHS gene. One CHS cDNA clone containing an open reading frame and a partial stilbene synthase (STS)cDNA, the stilbene-type phytoalexin, were isolated. The CHS cDNA clone (VCHS) showed 87% sequence homology with VvCHS (V.vinifea) and 72.3% identity with VSTSY(V.vinifea). its amino acid sequences were longer than any other CHS genes as 454 residues. Two genes were weakly expressed in white light irradiated cells, but highly induced in UV-B irradiated condition during 32 hours. Interestingly, the STS was quickly and abundantly expressed from 2 hours when supplemented with jasmonic acid (JA) and the maximum expression was observed at 4 hours and then gradually decreased. But, the additional UV-B or white light quickly degraded the STS expression than only JA treated grape suspension cells. The CHS also was rapidly induced with JA and the synergistical effect was observed at the addigional light treatment of UV-B or white light. These results are indicated that CHS and STS have different response mechanisms against the environmental stresses.

• Journal of Microbiology and Biotechnology
• /
• 제15권6호
• /
• pp.1408-1413
• /
• 2005

Elevated expression of carcinoembryonic antigen (CEA) has been implicated in various biological aspects of neoplasia such as tumor cell adhesion, metastasis, blocking of cellular immune mechanisms, and antiapoptosis function. Thus, the CEA could be an important target for anticancer therapy. In this study, we developed Tetrahymena group 1 intron-based trans-splicing ribozymes that can specifically target and replace CEA RNA. To this end, we first determined which regions of the CEA RNA were accessible to ribozymes by employing an RNA mapping strategy that was based on a trans-splicing ribozyme library. Next, we assessed the ribozyme activities by comparing the trans-splicing activities of several ribozymes that targeted different regions of the CEA RNA, and then the ribozyme that could target the most accessible site was observed to be the most active with high fidelity in vitro. Moreover, the specific trans-splicing ribozyme was found to react with and altered the target CEA transcripts in mammalian cells with high fidelity. These results suggest that the Tetrahymena ribozyme can be utilized to replace CEA RNAs in tumors with a new RNA-harboring anticancer activity, thereby hopefully reverting the malignant phenotype.

• Food Science and Biotechnology
• /
• 제18권4호
• /
• pp.954-958
• /
• 2009

Effects of controlled- and uncontrolled-pH operations on phenylalanine ammonia lyase (PAL) production by a recombinant Escherichia coli strain were investigated at uncontrolled-pH ($pH_{UC}$) and controlled-pH ($pH_C$) of 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, and 8.5 in bioreactor systems. The results showed that the recombinant PAL activity was improved significantly by controlled pH strategy. Among the $pH_C$ operations, the highest PAL activities were obtained under $pH_C$ 7.5 strategy where cell mass ($OD_{600\;nm}$) and PAL activity was 1.3 and 1.8 fold higher than those of $pH_{UC}$, respectively. The maximum PAL activity reached 123 U/g. The $pH_C$ 7.5 strategy made recombinant plasmid more stable and therefore allowed easier expression of PAL recombinant plasmid, which increased PAL production. It was indicated that the new approach (controlled-pH strategy) obtained in this work possessed a high potential for the industrial production of PAL, especially in the biosynthesis of L-phenylalanine.

• Journal of Microbiology and Biotechnology
• /
• 제19권9호
• /
• pp.881-887
• /
• 2009

Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78% of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 168 rRNA sequence. The EPA content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. A cosmid DNA including the EPA biosynthesis gene cluster consisting of pfaA-E was isolated from a cosmid library of genomic DNA of Shewanella sp. BR-2, named pCosEPA-BR2. An E. coli clone harboring pCosEPA-BR2 produced EPA at a maximum level of 7.5% of total fatty acids, confirming the EPA biosynthesis activity of the cloned gene cluster.

• 한국정보통신학회논문지
• /
• 제16권12호
• /
• pp.2725-2730
• /
• 2012

본 논문에서는 H.264/AVC 인코더의 성능 향상을 위해 다중 참조 프레임 기법과 묵시적 가중 예측 기법을 이용하고 낮은 외부 메모리 접근율을 위해 이전 참조 프레임 데이터를 재사용하는 인터 예측기 하드웨어 구조를 제안한다. 참조 소프트웨어JM16.0과 비교하여 참조 프레임 접근율이 약 24%만큼 감소하고 참조 영역 메모리가 약 46%만큼 감소하였다. 통합 구조는 Verilog HDL로 설계되고 Magnachip 0.18um공정으로 합성한 결과 게이트 수는 약 2,061k 이고 91Mhz로 동작한다.

• 한국통신학회논문지
• /
• 제24권9A호
• /
• pp.1442-1450
• /
• 1999

본 논문에서는 MPEG-2 비디오 부호화기에서의 프레임 메모리 하드웨어 구현을 위한 DRAM의 효율적인 메모리 맵과 이에 따른 하드웨어 구조를 제안한다. 논문에서 제시된 메모리 맵은 DRAM 사용 시간과 대역폭을 줄임으로써 시스템의 성능을 개선할 수 있도록 설정되었고, 개발된 하드웨어는 MPEG-2 비디오 부호화기에 구성된 타모듈과 인터페이스를 위해 하드웨어는 VHDL을 이용하여 구현하였으며, $0.5\mu\textrm{m}$, VTI, ASIC 라이브러리인 camn5a3을 이용하여 합성하였다. 개발된 하드웨어에 대한 RT (register transfer) 수준 및 게이트 수준의 검증을 위해 VHDL 시뮬레이터와 로직 합성 툴을 사용하였고, 추후 성능 개선과 기능 검증을 위해서 하드웨어 에뮬레이터를 개발하였다. 구현된 하드웨어 MPEG-2의 MP@ML에서 요구하는 전송률로 데이터를 채널에 전송할 수 있도록 하였다.

• 상하수도학회지
• /
• 제26권3호
• /
• pp.399-408
• /
• 2012

The overall goal of this study was to characterize and quantify ammonia-oxidizing bacteria (AOB) in four different full-scale sequence batch reactor (SBR) wastewater treatment plants. Also, this study focused on assessing the occurrence of the alternative ammonia-oxidizing microbes such as anammox (anaerobic ammonia oxidation) bacteria (AMX) and ammonia-oxidizing archaea (AOA) in these systems. Based on total AOB numbers and the estimated cell density in the mixed liquor samples, AOB constituted 0.3 - 1.8% of the total bacterial population in the four WWTPs. Based on clone library, Nitrosomonas ureae-like AOB were dominant in plant A and B, while plant C and D had Nitrosomonas nitrosa-like AOB as major AOB group. The four different AMX primer sets targeting AMX 16S rRNA gene produced PCR amplicons distantly related to Chlamydia and Planctomycetales group bacteria. However, it was not clear these groups of bacteria perform anammox reaction in the SBR plants. Also, molecular evidence of AOA was found in one of the SBR plants, with a sequence located in the deep branch of the sediment creanarchaeota group.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 추계학술대회
• /
• pp.92-92
• /
• 2018

Silene seulensis Nakai was used as traditional medicines in Korea, we collected plant from demilitarized zone (DMZ). S. seulensis was extracted with 30, 50, and 70% ethanol and partitioned successively with n-hexane, EtOAc, dichloromethane and BuOH. These extracts (30, 50 and 70% ethanol) were evaluated the cytotoxicity on B16F10 and Hacat cell lines. The LC-MS/MS data of each fractions (n-hexane, EtOAc, dichloromethane, and BuOH) were compared with MS library, combined with ultraviolet/visual (UV/Vis) and MS data for faster determine structure by database search results. This led to the identification of four compounds (1-4) from S. seulensis. These compounds was isolated first time from S. seulensis. Their chemical structures are elucidated by combinations of NMR and mass spectrometry techniques.

• 전자공학회논문지A
• /
• 제33A권10호
• /
• pp.186-194
• /
• 1996

The SliM (sliding memory plane) array processor has been proposed to alleviate disadvantages of existing mesh-connected SIMD(single instruction stream- multiple data streams) array processors, such as the inter-PE(processing element) communication overhead, the data I/O overhead and complicated interconnections. This paper presents the deisgn and implementation of SliM image processor ASIC (application specific integrated circuit) chip consisting of mesh connected 5 X 5 PE. The PE architecture implemented here is quite different from the originally proposed PE. We have performed the front-end design, such as VHDL (VHSIC hardware description language)modeling, logic synthesis and simulation, and have doen the back-end design procedure. The SliM ASIC chip used the VTI 0.8$\mu$m standard cell library (v8r4.4) has 55,255 gates and twenty-five 128 X 9 bit SRAM modules. The chip has the 326.71 X 313.24mil$^{2}$ die size and is packed using the 144 pin MQFP. The chip operates perfectly at 25 MHz and gives 625 MIPS. For performance evaluation, we developed parallel algorithms and the performance results showed improvement compared with existing image processors.