• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.5A
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• pp.554-565
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• 2004

In this paper, we implement single cycle and multi cycle adders. We can compare area and time by using the implemented adders. The size of adders is 64, 128, 256-bits. The architecture of hybrid adders is that the carry-out of small adder groups can be interconnected by utilizing n carry propagate unit. The size of small adder groups is selected in three formats - 4, 8, 16-bits. These adders were implemented with Verilog HDL with top-down methodology, and they were verified by behavioral model. The verified models were synthesized with a Samsung 0,35(um), 3.3(V) CMOS standard cell library while a using Synopsys Design Compiler. All adders were synthesized with group or ungroup. The optimized adder for a Crypto-processor included Smart Card IC is that a 64-bit RCA based on 16-bit CLA. All small adder groups in this optimized adder were synthesized with group. This adder can operate at a clock speed of 198 MHz and has about 961 gates. All adders can execute operations in this won case conditions of 2.7 V, 85 $^{\circ}C$.

• Proceedings of the Korean Society of Crop Science Conference
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• 1999.05a
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• pp.162-163
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• 1999

The goal of this research was (1) to describe the conditions and parameters required for the cell cycle synchronization and the accumulation of large number of metaphase cells in maize and other cereal root tips, (2) to isolate intact metaphase chromosomes from root tips suitable for characterization by flow cytometry, and (3) to construct chromosome-specific libraries from maize. Plant metaphase chromosomes have been successfully synchronized and isolated from many cereal root-tips. DNA synthesis inhibitor (hydroxyurea) was used to synchronize cell cycle, follwed by treatement with trifluralin to accumulate metaphase chromosomes. Maize flow karyotypes show substantial variation among inbred lines. thish variation should be sueful in isolating individual chromosome types. In addition, flow cytometry is a useful method to measure DNA content of individual chromosomes in a genotyps, and to detect chromosomal variations. Individual chromosome peaks have been sorted from the maize hybrid B73/Mol7. Libraries were generated form the DOP-PCR amplification product from each peak. To date, we have analyzed clones from a library constructed from the maize chromosome 1 peak. Hybridization of labeled genomic DNA to clone inserts indicated that $24\%,\;18\%,\;and\;58\%$ of the clones were highly repetitive, medium repetitive, and low copy, respectively. Fifty percent of putative low cpoy clones showed single bands on inbred screening, blots, and the remaining $50\%$ were low copy repeats. Single copy clones showing polymorphism will be mapped using recombinant inbred mapping populations. Repetitive clones are being characterized by Southern blot analysis, and will be screened by in situ hybridization for their potential utility as chromosome specific markers.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.8A
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• pp.820-826
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• 2007

This paper proposes an enhanced degree computationless modified Euclid's(E-DCME) algorithm and its architecture for Reed-Solomon decoders. The proposed E-DCME algorithm has shorter critical path delay that is $T_{mult}+T_{add}+T_{mux}$ compared with the existing modified Euclid's algorithm and the degree computationless modified Euclid's(DCME) algorithm since it uses new initial conditions. The proposed E-DCME architecture employing a systolic array requires only 2t-1 clock cycles to solve the key equation without initial latency. In addition, the E-DCME architecture consisting of 3t basic cells has regularity and scalability since it uses only one processing element. The E-DCME architecture using the $0.18{\mu}m$ Samsung standard cell library consists of 18,000 gates.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.4A
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• pp.447-457
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• 2004

This paper proposes a RSA crypto-processor for embedded systems. The architecture of the RSA crypto-processor should be used relying on Big Montgomery algorithm, and is supported by configurable bit size. The RSA crypto-processor includes a RSA control signal generator, an optimal Big Montgomery processor(adder, multiplier). We use diverse arithmetic unit (adder, multiplier) algorithm. After we compared the various results, we selected the optimal arithmetic unit which can be connected with ARM core-processor. The RSA crypto-processor was implemented with Verilog HDL with top-down methodology, and it was verified by C language and Cadence Verilog-XL. The verified models were synthesized with a Hynix 0.25${\mu}{\textrm}{m}$, CMOS standard cell library while using Synopsys Design Compiler. The RSA crypto-processor can operate at a clock speed of 51 MHz in this worst case conditions of 2.7V, 10$0^{\circ}C$ and has about 36,639 gates.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.9A
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• pp.1332-1339
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• 2000

This paper presents the architecture and design of a high speed asymmetric data transmission baseband MODEM ASIC chip for CATV networks. The implemented MODEM chip supports the physical layer of the DOCSIS(Data Over Cable Service Interface Specification) standard in MCNS(Multimedia Cable Network System) The chip consists of a QPSK/16-QAM transmitter and a 64/256-QAM receiver which contain a symbol timing recovery circuit, a carrier recovery circuit, a blind equalizer using MMA and LMS algorithms. The chip can support data rates of 64Mbps at 256 QAM and 48Mbps at 64-QAM and can provide symbol rates up to 8MBaud. This symbol rate is faster than existing QAM receivers. We have performed logic synthesis using the $0.35\mu\textrm{m}$ standard cell library. The total number of gates is about 290,000 and the implemented chip is being fabricated and will be delivered soon.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.7B
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• pp.647-659
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• 2003

LPM(Longest Prefix Matching)searching in If address lookup is a major bottleneck of IP packet processing in the high speed router. In the conventional lookup table for the LPM searching in CAM(Content Addressable Memory) the complexity of fast update take 0(1). In this paper, we designed pipeline architecture for fast update of 0(1) cycle of lookup table and high throughput and low area complexity on LPM searching. Lookup-table architecture was designed by CAM(Content Addressable Memory)away that uses 1bit RAM(Random Access Memory)cell. It has three pipeline stages. Its LPM searching rate is affected by both the number of key field blocks in stage 1 and stage 2, and distribution of matching Point. The RTL(Register Transistor Level) design is carried out using Verilog-HDL. The functional verification is thoroughly done at the gate level using 0.35${\mu}{\textrm}{m}$ CMOS SEC standard cell library.

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1430-1435
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• 2020

Bacterial cellulose (BC) has outstanding physical and chemical properties, including high crystallinity, moisture retention, and tensile strength. Currently, the major producer of BC is Komagataeibacter xylinus. However, due to limited tools of expression, this host is difficult to engineer metabolically to improve BC productivity. In this study, a regulated expression system for K. xylinus with synthetic ribosome binding site (RBS) was developed and used to engineer a BC biosynthesis pathway. A synthetic RBS library was constructed using green fluorescent protein (GFP) as a reporter, and three synthetic RBSs (R4, R15, and R6) with different strengths were successfully isolated by fluorescence-activated cell sorting (FACS). Using synthetic RBS, we optimized the expression of three homologous genes responsible for BC production, pgm, galU, and ndp, and thereby greatly increased it under both static and shaking culture conditions. The final titer of BC under static and shaking conditions was 5.28 and 3.67 g/l, respectively. Our findings demonstrate that reinforced metabolic flux towards BC through quantitative gene expression represents a practical strategy for the improvement of BC productivity.

• Nuclear Engineering and Technology
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• v.47 no.7
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• pp.791-803
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• 2015

The effective cross sections (XSs) in the direct whole core calculation code nTRACER are evaluated by the equivalence theory-based resonance-integral-table method using the WIMS-based library as an alternative to the subgroup method. The background XSs, as well as the Dancoff correction factors, were evaluated by the enhanced neutron-current method. A method, with pointwise microscopic XSs on a union-lethargy grid, was used for the generation of resonance-interference factors (RIFs) for mixed resonant absorbers. This method was modified by the intermediate-resonance approximation by replacing the potential XSs for the non-absorbing moderator nuclides with the background XSs and neglecting the resonance-elastic scattering. The resonance-escape probability was implemented to incorporate the energy self-shielding effect in the spectrum. The XSs were improved using the proposed method as compared to the narrow resonance infinite massbased method. The RIFs were improved by 1% in $^{235}U$, 7% in $^{239}Pu$, and >2% in $^{240}Pu$. To account for thermal feedback, a new feature was incorporated with the interpolation of pre-generated RIFs at the multigroup level and the results compared with the conventional resonance-interference model. This method provided adequate results in terms of XSs and k-eff. The results were verified first by the comparison of RIFs with the exact RIFs, and then comparing the XSs with the McCARD calculations for the homogeneous configurations, with burned fuel containing a mixture of resonant nuclides at different burnups and temperatures. The RIFs and XSs for the mixture showed good agreement, which verified the accuracy of the RIF evaluation using the proposed method. The method was then verified by comparing the XSs for the virtual environment for reactor applicationbenchmark pin-cell problem, as well as the heterogeneous pin cell containing burned fuel with McCARD. The method works well for homogeneous, as well as heterogeneous configurations.

• BMB Reports
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• v.52 no.7
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• pp.445-450
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• 2019

TTYH2 is a calcium-activated, inwardly rectifying anion channel that has been shown to be related to renal cancer and colon cancer. Based on the topological prediction, TTYH2 protein has five transmembrane domains with the extracellular N-terminus and the cytoplasmic C-terminus. In the present study, we identified a vesicle transport protein, ${\beta}$-COP, as a novel specific binding partner of TTYH2 by yeast two-hybrid screening using a human brain cDNA library with the C-terminal region of TTYH2 (TTYH2-C) as a bait. Using in vitro and in vivo binding assays, we confirmed the protein-protein interactions between TTYH2 and ${\beta}$-COP. We also found that the surface expression and activity of TTYH2 were decreased by co-expression with ${\beta}$-COP in the heterologous expression system. In addition, ${\beta}$-COP associated with TTYH2 in a native condition at a human colon cancer cell line, LoVo cells. The over-expression of ${\beta}$-COP in the LoVo cells led to a dramatic decrease in the surface expression and activity of endogenous TTYH2. Collectively, these data suggested that ${\beta}$-COP plays a critical role in the trafficking of the TTYH2 channel to the plasma membrane.

• Mycobiology
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• v.47 no.2
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• pp.242-249
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• 2019

Betaine derivatives are considered major ingredients of shampoos and are commonly used as antistatic and viscosity-increasing agents. Several studies have also suggested that betaine derivatives can be used as antimicrobial agents. However, the antifungal activity and mechanism of action of betaine derivatives have not yet been fully understood. In this study, we investigated the antifungal activity of six betaine derivatives against Malassezia restricta, which is the most frequently isolated fungus from the human skin and is implicated in the development of dandruff. We found that, among the six betaine derivatives, lauryl betaine showed the most potent antifungal activity. The mechanism of action of lauryl betaine was studied mainly using another phylogenetically close model fungal organism, Cryptococcus neoformans, because of a lack of available genetic manipulation and functional genomics tools for M. restricta. Our genome-wide reverse genetic screening method using the C. neoformans gene deletion mutant library showed that the mutants with mutations in genes for cell membrane synthesis and integrity, particularly ergosterol synthesis, are highly sensitive to lauryl betaine. Furthermore, transcriptome changes in both C. neoformans and M. restricta cells grown in the presence of lauryl betaine were analyzed and the results indicated that the compound mainly affected cell membrane synthesis, particularly ergosterol synthesis. Overall, our data demonstrated that lauryl betaine influences ergosterol synthesis in C. neoformans and that the compound exerts a similar mechanism of action on M. restricta.