• Asian-Australasian Journal of Animal Sciences
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• v.7 no.4
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• pp.547-554
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• 1994

This paper presents the effects of supplementary feeding and internal parasites on the growth rates of female weaner goats raised under improved management. A completely randomized $3{\times}3{\times}2$ factorial design was used. Factors were genotype (Thai native: TN, 75% TN $\times$ 25% Anglo-Nubian: An and 50% TN $\times$ 50% AN), feeding grazing only, low (1.0% BW/d) and high (1.5% BW/d) supplementation and parasite control (undrenched and drenched). It was shown that native goats had significantly (p<0.05) higher growth rates than did the cross-bred goats from 12-24 weeks of the trial. The growth rate of goats grazing improved pasture depended on the amount of concentrate offered as a supplement. There was no significant difference in growth rates between undrenched and drenched goats. There was no interaction effect on growth rates between the treatments. Drenched goats had significantly (p<0.01) lower egg counts per gram of gastro-intestinal nematode than did undrenched goats. There was no significant difference between the treatments for blood constituents (total protein, haemoglobin, packed cell volume, eosinophils, lymphocytes, monocytes and basophils).

• Journal of Microbiology and Biotechnology
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• v.30 no.1
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• pp.101-108
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• 2020

Infections by herpes simplex viruses have an immense impact on humans, ranging from self-limiting, benign illness to serious, life-threatening diseases. While nucleoside analog drugs are available, resistance has been increasing and currently no vaccine exists. Ginsenosides derived from Panax ginseng have been documented to inhibit several viruses and bolster immune defenses. This study evaluated 12 of the most relevant ginsenosides from P. ginseng for toxicities and inhibition of herpes simplex viruses types 1 and 2 in Vero cells. The effects of test compounds and virus infection were determined using a PrestoBlue cell viability assay. Time course studies were also conducted to better understand at what points the virus life cycle was affected. Non-toxic concentrations of the ginsenosides were determined and ranged from 12.5 μM to greater than 100 μM. Ginsenoside 20(S)-Rg3 demonstrated the greatest inhibitory effect and was active against both HSV-1 and HSV-2 with an IC₅₀ of approximately 35 μM. The most dramatic inhibition-over 100% compared to controls-occurred when the virus was exposed to 20(S)-Rg3 for 4 h prior to being added to cells. 20(S)-Rg3 holds promise as a potential chemotherapeutic agent against herpes simplex viruses and, when used together with valacyclovir, may prevent increased resistance to drugs.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.1
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• pp.37-42
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• 1981

Ichthyophonus disease broke out among rainbow trout (Salmon gairdneri) fry in November 1979, and after that a fish group containing diseased fish was kept for one year. The authors examined histopathologically the diseased fish 3 times, at 6 momths intervals. Diseased fish showed markedly stunted growth and darkish coloration, and anatomically the liver with small white nodules, the heart with red nodules, the spleen with granular nodules and the markedly tumefied kidney. Ichthyophonus invaded various tissues in the host and took the shape of multinucleate spherical or hyphal body. The multinucleate-spherical bodies contained many nuclei with a large nucleolus and granular cytoplasm. Histopathologically systemic dissemination and systemic proliferation by Ichthyophonus sp. wire indentified. In addition to a single multinucleate-spherical body appeared in the various tissue, large granulomas containing two to several generations of Ichthyophonus sp. were formed especially in the liver, kidey and spleen. The inflammatory response against Ichthyophonus was characterized by mononuclear-celluar proliferation with giant cell formation and fibrobasts proliferation.

• The Korean Journal of Pain
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• v.26 no.3
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• pp.242-248
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• 2013

Varicella (chickenpox) is a highly contagious airborne disease caused by primary infection with the varicella zoster virus (VZV). Following the resolution of chickenpox, the virus can remain dormant in the dorsal sensory and cranial ganglion for decades. Shingles (herpes zoster [HZ]) is a neurocutaneous disease caused by reactivation of latent VZV and may progress to postherpetic neuralgia (PHN), which is characterized by dermatomal pain persisting for more than 120 days after the onset of HZ rash, or "well-established PHN", which persist for more than 180 days. Vaccination with an attenuated form of VZV activates specific T-cell production, thereby avoiding viral reactivation and development of HZ. It has been demonstrated to reduce the occurrence by approximately 50-70%, the duration of pain of HZ, and the frequency of subsequent PHN in individuals aged ${\geq}50$ years in clinical studies. However, it has not proved efficacious in preventing repeat episodes of HZ and reducing the severity of PHN, nor has its long-term efficacy been demonstrated. The most frequent adverse reactions reported for HZ vaccination were injection site pain and/or swelling and headache. In addition, it should not be administrated to children, pregnant women, and immunocompromised persons or those allergic to neomycin or any component of the vaccine.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.78-83
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• 1999

Thirty-six environmental isolates of Vibrio vulnificus obtained from seawater, sediments, and raw seafoods, and 18 clinical isolates from Vibrio septicemia patients were typed by restriction endonuclease digestion profiles (REDP) of genomic DNA with SfiI. The results revealed a high-level of variation in REDPs, indicating a vast genomic diversity among V. vulnificus strains. Genetic relatedness of the strains showed similarities ranging from 10％ to 100％. Different REDPs for isolates from various raw seafoods were obtained, and clustering of strains according to type of seafoods was not observed. In contrast, clinical isolates of V. vulnificus showed higher similarity to one another, and could be subdivided into one separate group. The difference in REDPs of the V. vulnificus isolates from clinical origin and from raw seafoods substantiates the previous observation that only a single type of pathogenic strain was involved in each human infection, despite the numerous genetically polymorphic strains found from implicated oysters.

• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1908-1915
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• 2018

Upon viral infection, the host cell recognizes the invasion through a number of pattern recognition receptors. Melanoma differentiation associated gene 5 (MDA5) and retinoic acid-inducible gene-I (RIG-I) recognize RNA molecules derived from invading viruses, activating down-stream signaling cascades, culminating in the induction of the type I interferon. On the other hand, viruses have evolved to evade type I interferon-mediated inhibition. Hepatitis E virus has been shown to encode a few antagonists of type I interferon and it is not surprising that viruses encode multiple mechanisms of viral evasion. In the present study, we demonstrated that HEV PCP strongly down-regulates MDA5-mediated activation of interferon ${\beta}$ induction in a dose-dependent manner. Interestingly, MDA5 protein expression was almost completely abolished. In addition, polyinosinic polycytidylic acid (poly(I:C))- and Sendai virus-mediated activation of type I interferon responses were similarly abrogated in the presence of HEV PCP. Furthermore, HEV PCP down-regulates several molecules that play critical roles in the induction of type I IFN expression. Taken together, these data collectively suggest that HEV-encoded PCP is a strong antagonist of type I interferon.

• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1946-1954
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• 2018

The aim of this study was to isolate and characterize a lytic Yersinia enterocolitica-specific phage (KFS-YE) as a biocontrol agent. KFS-YE was isolated and purified with the final concentration of ($11.72{\pm}0.03$) log PFU/ml from poultry. As observed by transmission electron microscopy, KFS-YE consisted of an icosahedral head and a contractile tail, and was classified in the Myoviridae family. KFS-YE showed excellent narrow specificity against Y. enterocolitica only. Its lytic activity was stable at wide ranges of pH (4-11) and temperature ($4-50^{\circ}C$). The latent period and burst size of KFS-YE were determined to be 45 min and 38 PFU/cell, respectively. KFS-YE showed relatively robust storage stability at -20, 4, and $22^{\circ}C$ for 40 weeks. KFS-YE demonstrated a bactericidal effect in vitro against Y. enterocolitica and provided excellent efficiency with a multiplicity of infection as low as 0.01. This study demonstrated the excellent specificity, stability, and efficacy of KFS-YE as a novel biocontrol agent. KFS-YE may be employed as a practical and promising biocontrol agent against Y. enterocolitica in food.

• Journal of Microbiology and Biotechnology
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• v.12 no.1
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• pp.65-70
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• 2002

A TGF-${\beta}1$/GFP monomeric fusion protein was cloned from pPK9A and pGFP-Cl plasmid by PCR amplification. The fusion protein was expressed in a $Bac-To-Bac^{TM}$ baculovirus expression system. A 45 kDa fusion protein was purified using an Ni-NTA column with 300 mM imidazol from a cell lysate infected with recombinant viruses for 72 h post-infection. The fusion protein cross-reacted with the commercial $TGF-{\beta}1$ polyclonal Ab as well as Ab raised against a precursor, monomeric $TGF-{\beta}1$, and GFP. The binding activity of the fusion protein with a $TGF-{\beta}1$ receptor was examined. Fluorescence was observed in Mv1Lu cells, yet not in insect cells treated with the fusion protein. No fluorescence was detected in Mv1Lu cells incubated with the fusion protein treated with Ab prior to the binding reaction, or with GFP alone, thereby indicating that the binding of the fusion protein was specific to $TGF-{\beta}1$ with a receptor.

• Korean Journal of Veterinary Service
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• v.36 no.1
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• pp.37-44
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• 2013

The ecologically and economically important honeybee species are susceptible to infections by various pathogens. This study was investigated to detect infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province by PCR in 2010~2011. A total of 11 infectious pathogens, including 6 viruses, 2 bacteria, 2 fungi, and 1 parasite, were investigated in honeybee colonies suffering from symptoms of sudden collapse, depopulation, or paralysis. The infectious pathogens and infection rates among 24 honeybee colonies detected were as follows: sacbrood virus (66.7%), deformed wing virus (4.2%), black queen cell virus (12.5%), Kashmir bee virus (29.2%), American foulbrood (41.7%), European foulbrood (12.5%), stonebrood (45.8%), chalkbrood (4.2%), and Nosema (33.3%), respectively. Since the coinfection rates of multiple pathogens were detected high in honeybee colonies reared in eastern Gyeongbuk province, large-scale investigation and appropriate control programs need to be established in this region.

• The Journal of Korea Assosiation for Disability and Oral Health
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• v.3 no.1
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• pp.26-30
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• 2007

Leukocyte adhesion deficiency(LAD) is a rare autorecessive defect of phagocytic function resulting from a lack of leukocyte cell surface expression of ${\beta}_2$ integrin molecules(CD 18) that are essential for leukocyte adhesion to endothelial cells and chemotaxis. As a results, patients with LAD suffer from severe bacterial infections and impaired wound healing. A small number of patients with leukocyte adhesion deficiency-1 have a milder defect, with residual expression of CD18. These patients tend to survive beyond infancy; they manifest progressive severe periodontitis, alveolar bone loss, periodontal pocket formation, and partial or total premature loss of the primary and permanent dentitions. In this report, we report on a 7 year old girl with severe oral involvement. The most import focus should be to control infections to reduce the risk for future infection.