• 산업공학
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• 제15권4호
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• pp.325-337
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• 2002

In this study we consider the problem of production planning of TFT-LCD(Thin Film Transistor - Liquid Crystal Display) production factory. Due to the complexities of the TFT-LCD production processes, it is difficult to schedule the production planning, and the study about automated scheduler is insufficient. In addition, the existing production method is a Push-System to raise the operation rate with expensive equipment, that has the problem to satisfy the due-date. This study presents an algorithm having a concept of Pull-System that satisfies the due-date and considers specialties of TFT-LCD production process. We make MPS(Master Production Schedule) according to the sales order, and present algorithms for scheduling about In/Out plan considering factory capacity, line balancing, material requirement, and inventory level of all Array, Cell, and Module processes. These algorithms are integrated as an automated production system, and we implement them in the actual TFT-LCD factory circumstance.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1154-1163
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• 2024

Glucosylation is a well-known approach to improve the solubility, pharmacological, and biological properties of flavonoids, making flavonoid glucosides a target for large-scale biosynthesis. However, the low yield of products coupled with the requirement of expensive UDP-sugars limits the application of enzymatic systems for large-scale. C. glutamicum is a Gram-positive and generally regarded as safe (GRAS) bacteria frequently employed for the large-scale production of amino acids and biofuels. Due to the versatility of its cell factory system and its non-endotoxin producing properties, it has become an attractive system for the industrial-scale biosynthesis of alternate products. Here, we explored the cell factory of C. glutamicum for efficient glucosylation of flavonoids using apigenin as a model flavonoid, with the heterologous expression of a promiscuous glycosyltransferase, YdhE from Bacillus licheniformis and the endogenous overexpression of C. glutamicum genes galU1 encoding UDP-glucose pyrophosphorylase and pgm encoding phosphoglucomutase involved in the synthesis of UDP-glucose to create a C. glutamicum cell factory system capable of efficiently glucosylation apigenin with a high yield of glucosides production. Consequently, the production of various apigenin glucosides was controlled under different temperatures yielding almost 4.2 mM of APG1(apigenin-4'-O-β-glucoside) at 25℃, and 0.6 mM of APG2 (apigenin-7-O-β-glucoside), 1.7 mM of APG3 (apigenin-4',7-O-β-diglucoside) and 2.1 mM of APG4 (apigenin- 4',5-O-β-diglucoside) after 40 h of incubation with the supplementation of 5 mM of apigenin and 37℃. The cost-effective developed system could be used to modify a wide range of plant secondary metabolites with increased pharmacokinetic activities on a large scale without the use of expensive UDP-sugars.

• 산업공학
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• 제12권1호
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• pp.10-18
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• 1999

Job shop manufacturing environments are using the concept of cellular manufacturing systems(CMS) which has several advantages in reducing production lead times, setup times, work-in-process, etc. Utilizing the similarities between cell-machine, part-machine, and the shape/size of parts, CMS can group machines and parts resulting in improved efficiency of this system. However, when grouping machines and parts in machine cells, there inevitably occurs exceptional elements(EEs), which can not operate in the same machine cell. Minimizing these EEs in CMS is a critical point that improving production efficiency. Constraints in machine duplication cost, machining process technology, machining capability, and factory space limitations are main problems that prevent achiving the goal of maintaining an ideal CMS environment. This paper presents an algorithm that minimizes EEs under the constraints of machine duplication cost and factory space limitation. Developing exceptional operation similarity(EOS) by cell-machine incidence matrix and part-machine incidence matrix, it brings the machine cells that operate the parts or not. A mathematical model to minimize machine duplication is developed by EOS, followed by a heuristic algorithm in order to reflect dynamic situation resulting from minimizing exceptional elements process and the mathematical model. A numerical example is provided to illustrate the algorithm.

• Journal of Microbiology and Biotechnology
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• 제28권3호
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• pp.367-374
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• 2018

RNA interference provides an effective tool for developing antitumor therapies. Cell-penetrating peptides (CPPs) are delivery vectors widely used to efficiently transport small-interfering RNA (siRNA) to intracellular targets. In this study, we investigated the efficacy of the cancer-specific CPP carrier BR2 to specifically transport siRNA to cancer-target cells. Our results showed that BR2 formed a complex with anti-vascular endothelial growth factor siRNA (siVEGF) that exhibited the appropriate size and surface charge for in vivo treatment. Additionally, the BR2-VEGF siRNA complex exhibited significant serum stability and high levels of gene-silencing effects in vitro. Moreover, the transfection efficiency of the complex into a cancer cell line was higher than that observed in non-cancer cell lines, resulting in downregulated intracellular VEGF levels in HeLa cells and comprehensively improved antitumor efficacy in the absence of significant toxicity. These results indicated that BR2 has significant potential for the safe, efficient, and specific delivery of siRNA for diverse applications.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1996년도 한국자동제어학술회의논문집(국내학술편); 포항공과대학교, 포항; 24-26 Oct. 1996
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• pp.1245-1248
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• 1996

This paper describes a cell programming environment that deals with problems associated with programming Flexible Manufacturing Cells(FMCs). The environment consists of the cell programming editor and the automatic generation module. In the cell programming editor, cell programmers can develop cell programs using task level description set which supports task-oriented specifications for manipulation cell activities. This approach to cell programming reduces the amount of details that cell programmers need to consider and allows them to concentrate on the most important aspects of the task at hand. The automatic generation module is used to transform task specifications into executable programs used by cell constituents. This module is based on efficient algorithm and expert systems which can be used for optimal path planning of robot operations and optimal machining parameters of machine tool operations. The development tool in designing the environment is an object-oriented approach which provides a simple to use and intuitive user interface, and allows for an easy development of object models associated with the environment.

• 한국정밀공학회지
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• 제14권7호
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• pp.126-136
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• 1997

This paper describes a task level cell programming environment that deals with difficulties in programming Flexible Manufacturing Cells (FMCs), and consists of the cell programming editor and the automatic generation module. In the cell programming editor, cell programs can be developed with task- oriented cell specifications that reduces the amount of details to be considered by cell programmers. The automatic generation module transforms task specifications into executable programs used by cell constituents. The development tool in designing the environment is an object-oriented approach which provides a simple to use and intuitive user interface, and allows for an easy development of object models associated with the environment. Test results are illustrated in order to demonstrate the applicability of the developed envifonment.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 추계학술대회
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• pp.27-31
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• 2002

Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The $paclitaxel-Genexol^{TM}$ is commercially available in Korea, and it will be launched to world market including USA after approval US FDA.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.27-31
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• 2002

Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The $paclitaxel-Cenexol^{TH}-is$ commercially available in Korea, and it will be launched to world market including USA after approval of US FDA.

• Journal of Plant Biotechnology
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• 제29권1호
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• pp.59-62
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• 2002

Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The paclitaxel-Genexol$^{TM}$-is commercially available in Korea, and it will be launched to world market including USA after approval of US FDA.

• 한국정밀공학회지
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• 제12권4호
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• pp.88-99
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• 1995

The demand for automatic manufacturing systems is increasing. Flexible Manufacturing System(FMS) is usually considered as a soluting for the shop floor automation. One of the difficulties in FMS is the communications problem. Since various machineries with different communications protocoles are included, applying a unified scheme is almost impossible. Therefore, a systematic approach is a key point to solve the communication problem. A cell is difined as an automation unit where closely related for a job reside together. A cell is a messenger between upper level computers and lower level machine equipment. In this research, the fonctions of the cell are defined to have more capabilities than conventional cells since a cell can be often a total manufacturing system is a small to medium sized factory. The cell conducts communications with different machines through the communications schemes established here. A set of software system has been developed according to the defined communication. The software has been tested for a simulation and real experiments for proof.