• 제목/요약/키워드: cell density

검색결과 3,237건 처리시간 0.03초

피뿔고동 ( Rapana venosa Valenciennes )의 Osphardium 에 관한 형태학적 연구 (Morphological Study on the Osphradium of Rapana venosa (Gastropoda : Muricidae))

  • 이정재;김성훈
    • 한국패류학회지
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    • 제4권1호
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    • pp.1-16
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    • 1988
  • The authors observed histochemical and ultrastructural characters on the osphradium of Rapana venosa Valenciennes using light microscope, scanning and transmission electron microscpes. The results were as follows:1)The basic structure of osphradium was bipectinated shape, which consisted of a septum situating in the center of osphradium and numerous osphradial leaflets. On the other hand, Epidermis of ospradial leaflets formed the structure of pseudostratified ciliated columnar epithelium which was composed of an epithelial cell layer, a basal cel layer and a neuropile. 2) Ciliated dpithelial cells:A large number of these cells were observed on the lateral and ventral regions but a small number of them were observed on the dorsal region. These cells had cylindrical microvilli, slender mitochondria and serve fibers.3) Supporting cells: These cells had cylindrical microvilli, spongy layer, electron dense granules, mitochondria and nerve fibers4) Four types secretory epothelial cells: Four distinct types of secretory epithelial cells were recognized and were arbitrily designated as Type I, Type II, Type III and Type IV.cell type I: These cells contained electron denwe granules(diameter, 0.94-1.56${\mu}{\textrm}{m}$), well developed Golgi apparatus and rough endoplasmic reticula, cell type II: These cills contained two types of granules of the different electron density. One was high electron density granules which were 0.4-1.0${\mu}{\textrm}{m}$ in diameter, The other was low electron density granules which were 0.75-1.2${\mu}{\textrm}{m}$ in diameter.cell type III:These cells had fibrous secretory materials and exhibited strongly positive reaction with Toluidine blue.cell type IV:A large number of this type of cells were observed on the ventral region of ospgradial leaflets and positively reacted with periodic acid Schiff reagent. 5)Dark cells contained several electron dense cillaty rootlets and unmerous granules but cellular organelles were not observed.6) Four types basal cells: Four distinci types of basal cells were recognized and arbitrarily designated as Type I, Type II, Type III and Type IV.Cell type I(light cell): These cells exhibited low electuon density and contained short smooth endoplasmic reticula, several vacuoles and granules.

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셀 기반 유한 차분법을 이용한 효율적인 3차원 음향파 파동 전파 모델링 (Efficient 3D Acoustic Wave Propagation Modeling using a Cell-based Finite Difference Method)

  • 박병경;하완수
    • 지구물리와물리탐사
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    • 제22권2호
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    • pp.56-61
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    • 2019
  • 셀 기반 유한 차분법을 사용하여 P파 속도와 밀도 변화를 고려한 3차원 시간 영역 음향 파동 전파 모델링에서 성능을 향상시킬 수 있는 방법을 살펴보았다. 일반적인 유한 차분법에서는 격자점에 탄성파 속도 또는 밀도와 같은 물성을 할당하고 계산하지만 셀 기반 유한 차분법에서는 이러한 물성을 격자점 사이의 셀에 할당한다. 격자점에서의 차분식 계산을 위해서는 주변 셀의 물성 평균값을 이용하는데 이로 인해 일반적인 유한 차분법에 비해 계산량이 증가하게 된다. 이 연구에서는 이러한 계산량 문제를 개선하기 위해 메모리를 추가로 사용하여 모델링 시간을 30 % 이상 줄일 수 있었다. 또한 밀도가 제한적으로 변화하는 매질에서 셀 기반 유한 차분법과 일반 유한 차분법을 함께 사용하여 모델링 성능을 추가로 향상시킬 수 있었다.

Polymer Materials for Polymer Electrolyte Fuel Cells: Sulfonated Poly(ether sulfone)s for Fuel Cell Membranes

  • Kim, H.J.;Lee, S.Y.;Cho, E.;Ha, H.Y.;Oh, I.H.;Lim, T.H.
    • 한국고분자학회:학술대회논문집
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    • 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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    • pp.185-185
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    • 2006
  • The performances of proton exchange membrane fuel cell (PEMFC), direct formic acid fuel cell (DFAFC) and direct methanol fuel cell (DMFC) with sulfonated poly(ether sulfone) membrane are reported. Pt/C was coated on the membrane directly to fabricate a MEA for PEMFC operation. A single cell test was carried out using $H_2/air$ gases as fuel and oxidant. A current density of $730\;mA/cm^2$ at 0.60 V was obtained at $70^{\circ}C$. Pt-Ru (anode) and Pt (cathode) were coated on the membrane for DMFC operations. It produced $83\;mW/cm^2$ of maximum power density. The sulfonated poly(ether sulfone) membrane was also used for DFAFC operation under several different conditions. It showed good cell performances for several different kinds of polymer electrolyte fuel cell applications.

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Growth Rate and Biomass Productivity of Chlorella as Affected by Culture Depth and Cell Density in an Open Circular Photobioreactor

  • Liang, Fang;Wen, Xiaobin;Geng, Yahong;Ouyang, Zhengrong;Luo, Liming;Li, Yeguang
    • Journal of Microbiology and Biotechnology
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    • 제23권4호
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    • pp.539-544
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    • 2013
  • The effects of culture depth (2-10 cm) and cell density on the growth rate and biomass productivity of Chlorella sp. XQ-200419 were investigated through the use of a self-designed open circular pond photobioreactor-imitation system. With increases in culture depths from 2 to 10 cm, the growth rate decreased significantly from 1.08 /d to 0.39 /d. However, the biomass productivity only increased slightly from 8.41 to 11.22 $g/m^2/d$. The biomass productivity (11.08 $g/m^2/d$) achieved in 4 cm culture with an initial $OD_{540}$ of 0.95 was similar to that achieved in 10 cm culture with an initial $OD_{540}$ of 0.5. In addition, the duration of maximal areal productivity at a 4 cm depth was prolonged from 1 to 4 days, a finding that was also similar to that of the culture at a 10 cm depth. In both cases, the initial areal biomass densities were identical. Based on these results and previous studies, it can be concluded that the influence of culture depth and cell density on areal biomass productivity is actually due to different areal biomass densities. Under suitable conditions, there are a range of optimal biomass densities, and areal biomass productivity reaches its maximum when the biomass density is within these optimal ranges. Otherwise, biomass productivity will decrease. Therefore, a key factor for high biomass productivity is to maintain an optimal biomass density.

고준위폐기물 차폐용 압축벤토나이트의 응력-변형률 거동 분석 (A Study on Stress-Strain Characteristics of Compacted Bentonite for High-Level Radioactive Waste Repository)

  • 김도현;정상섬
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2009년도 춘계 학술발표회
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    • pp.792-797
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    • 2009
  • The stress-strain characteristics of compacted bentonite are investigated using experimental triaxial compression test by Hoek-cell. Special attention given to various dry density and water absorption ratio. Based on the test results, it is shown that the stress-strain relationship of compacted bentonite is highly influenced by dry density and water absorption ratio. Also, characteristics of Bentonite is similar to the clay rather than sand. Strength of compressed Bentonite increases with higher dry density. It shows maximum strength value, if in a same condition with dry density and constrain pressure. So we determine that value as the optimistic moisture contents for the maximun strength of compressed Bentonite.

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과불소탄소화물과 새로운 임펠러를 사용한 생물반응기의 특성 (Characteristics of a Bioreactor Using Perfluorocarbons and a New Impeller)

  • 조무환;김용락정재학김정목
    • KSBB Journal
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    • 제6권3호
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    • pp.231-240
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    • 1991
  • Recently, developments of large scale and high density cell culture methods have been the objects of many researches, because the demand of various pharmaceutical products produced by animal cell culture has been rapidly increasing. The cell culture equipment should have the requirements such as sufficient oxygen transfer and mixing, low shear stress and surface tension, and small foaming. In order to develop a proper bioreactor meeting these requirements simultaneously, a perfluorocarbon having high solubility of oxygen was sprayed into the medium as an oxygen carrier instead of air. Also, a new impeller was developed and combined together with the perfluorocarbon spraying system so as to design a new bioreartor for cell cultivation. The new impeller had better characteristics of mixing and oxygen transfer than the paddle and cell-lift impellers based on the same, shear rate. But, it was observed that the volumetric oxygen transfer coefficient of the new bioreactor decreased with increasing cell density during E. coli fermentation.

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Diode Equivalent Parameters of Solar Cell

  • Iftiquar, Sk Md;Dao, Vinh Ai;Yi, Junsin
    • Current Photovoltaic Research
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    • 제3권4호
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    • pp.107-111
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    • 2015
  • Current characteristic curve of an illuminated solar cell was used to determine its reverse saturation current density ($J_0$), ideality factor (n) and resistances, by using numerical diode simulation. High efficiency amorphous silicon, heterojunction crystalline Si (HIT), plastic and organic-inorganic halide perovskite solar cell shows n=3.27 for a-Si and n=2.14 for improved HIT cell as high and low n respectively, while the perovskite and plastic cells show n=2.56 and 2.57 respectively. The $J_0$ of these cells remain within $7.1{\times}10^{-7}$ and $1.79{\times}10^{-8}A/cm^2$ for poorer HIT and improved perovskite solar cell respectively.

회전식 여과기를 이용한 고농도 동물세포배양의 수학적 해석 (Mathematical Analysis of a High Density Animal Cell Culture with a Spin-Filter)

  • 박흥우
    • KSBB Journal
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    • 제9권2호
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    • pp.230-237
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    • 1994
  • 회전식 여과기는 일종의 분리기로서 동물세포의 연속배양에 이용되어 높은 세포농도와 그와 비례한 높은 생산성을 가져다 준다. 회전식 여과기를 이용한 세포배양은 여러 인자들에 의해 세포농도의 변화가 결정되는데 이를 수학적으로 modeling하고 수치 모사와 sensitivity analysis를 통하여 조사하였다. 고농도 배양시 암모니아의 축적은 세포 성장을 크게 둔화시키고 최대세포농도도 따라서 낮게 된다. 운전 인자 중 세포유치율은 세포 성장속도와 최대세포농도의 크기에 가장 큰 영향을 끼침이 밝혀졌다. 비배지공급속도는 세포농도의 변화에 거의 영향을 끼치지 않으며 배지의 연속식공급과 계단식공급은 세포성장에 큰 차이를 보이지 않는다.

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복소임피던스법에 의한 인산형 연료전지용 전해질 매트릭스 특성 (Characteristics of Matrix Retaining Electrolyte in a Phosphoric Acid Fuel Cell Analyzed by A.C. Impedance Spectroscopy)

  • 윤기현;장재혁;허재호;김창수;김태희
    • 한국세라믹학회지
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    • 제32권2호
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    • pp.189-196
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    • 1995
  • Materials retaining electrolyte of a phosphoric acid fuel cell (PAFC) have been prepared with SiC powder to SiC whisker mixing ratios of 1:1, 1:2, 1:3, 1:4, 0:1 by a tape casting method. When 3wt% dispersant (sorbitan monooleate) is added to a matrix, the porosity of the matrix decreases a little while the bubble pressure and area of the matrix increase remarkably in comparison with no dispersant content. Effect of the electrolyte resistance and the polarization resistance on perfomance of a PAFC has been investigated using A.C. impedance spectroscopy. With the increase of whisker content, the electrolyte resistance decreases due to the increase of porosity and acid absorbancy, and the polarization resistance increases due to the increase of surface roughness. The polarization resistance affects current density predominantly at the higher potential than 0.7V becuase the polarization resistance is considrably larger than the electrolyte resistance. Both the electrolyte resistance and the polarization resistance affect current density near 0.7V of the fuel cell operating potential because they have similar values. The electrolyte resistance affects current density predominantly at the lower potential than the fuel cell operating potential because the electrolyte resistance is larger than the polarization resistance.

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한국산 산민달팽이 (Incilaria fruhstorferi) 전립선의 미세구조 및 조직화학적 연구 (Ultrastructural and Histochemical Study on the Prostate Gland of a Korean Slug, Incilaria fruhstorferi)

  • 장남섭
    • Applied Microscopy
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    • 제27권2호
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    • pp.155-163
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    • 1997
  • Ultrastructural and histochemical studies were carried out on the prostate gland of a Korean slug (Incilaria fruhstorferi) with light and electron microscopes. The results are as follows. The endothelial tissue of the prostate gland is constituted with tall and narrow ciliated columnar cells, irregularly shaped cells and gland cells in which are included the elongated oval or ellipsoid nucleus those are surrounded by curved membrane. The endothelial tissue of the prostate gland is composed by type-A, B, C and type-D gland cells, and the glanules of type-A, B, and D gland cells are certified to neutral mucopolysaccharide because are related by PAS-alcian blue (pH 2.5) , but the other hand type-C gland cell is only weakly reacted to PAS, but strongly reacted by Million reaction. The glanules of type-A gland cell are small size (about $0.4{\mu}m$) and are seen high electron density, but the glanules of type-B gland cell are large size (about $0.7{\mu}m$) and glanule density is same type-A glanules. Long ellipsoid type-C gland cell contained round nucleus which is well developed beterochromatin in, and that small oval glanules (size, about $0.9{\mu}m$) of moderate high electron density which are formed a group of large glanule together with $4\sim5ea$, but type-D gland cell possessed round small nucleus are seen high electron dense glanules (size, $0.8{\mu}m$).

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